Volume 23 (2023): Edition 2 (April 2023)

The intestinal structure and gut microbiota are essential for the animals’ health. Chemical components taken with food provide the right environment for a specific microbiome which, together with its metabolites and the products of digestion, create an environment, which in turn affects the population size of specific bacteria. Disturbances in the composition of the gut microbiota can be a reason for the malformation of guts, which has a decisive impact on the animal’s health. This review aimed to analyse scientific literature, published over the past 20 years, concerning the effect of nutritional factors on gut health, determined by the intestinal structure and microbiota of monogastric animals. Several topics have been investigated: bioactive compounds (probiotics, prebiotics, organic acids, and herbal active substances), antibiotics and heavy metals (essential minerals and toxic heavy metals).

Cancer is a challenging ailment and represents the main reason for death worldwide for humans and animals. Although great developments have hindered cancer progression, several adverse effects are associated with modern chemotherapy. Natural remedies, such as the usage of medicinal plant or their products in cancer treatment, may decrease prejudicial side properties. Recently, the modern research scheme and innovative screening practices for herbs or plants have enabled phytochemical discovery for the prevention and treatment of cancer. This criticism highlights herbs such as acacia, basil, black seeds, cedar, castus, ficus, garlic, ginger, indigo, onion, pomegranate, quince, and thyme, promising anticancer effects. The present review also revealed the mode of action of each herb as anticancer effects at level in vitro and in vivo studies. The item also totalizes the vital mechanisms and signaling molecules involved in preventing cancer diseases. This will fill the investigation gap in the exploration of using natural molecules and encourage researchers in clinical trials of anticancer agents from herbs for humans and animals.

Pineapple is a commodity and economic fruit with a high market potential worldwide. Almost 60% of the fresh pineapple, such as peels, pulp, crowns and leaves, are agricultural waste. It is noteworthy that the waste has a high concentration of crude fibre, proteins, ascorbic acid, sugars and moisture content. The pineapple waste utilisation in animal feed has recently drawn the attention of many investigators to enhance growth performance and concomitantly reduce environmental pollution. Its inclusion in animal feed varies according to the livestock, such as feed block, pelleted or directly used as a roughage source for ruminants. The pineapple waste is also fermented to enrich the nutrient content of poultry feed. To date, the inclusion of pineapple waste in animal feed is optimistic not only for livestock but also for farmed fish. Indeed, it is an ideal strategy to improve the feed supply to the farm. This paper aims to overview the source, nutritional composition, and application of pineapple waste in animal feed. The recent findings on its effect on animal growth performance, nutrition and disease control are discussed comprehensively and summarised. The review also covers its benefits, potential impacts on sustainable farming and future perspectives.

Exosomes (EXOs) are naturally occurring nano-sized vesicles released into the extracellular environment by exocytosis. EXOs contribute to intercellular communications by regulating DNA, mRNA, and protein levels. EXOs are considered good vehicles for delivering therapeutic agents in multiple diseases. Camel milk (CM) is a valuable food with a rich source of EXOs. Camel milk exosomes (CMEXOs) have unique ingredients compared to other animal milks. It is reported that CMEXOs reduce the growth of cancer cells by inducing apoptosis, and inhibiting oxidative stress and inflammation. CMEXOs can block stress signaling pathways including inflammation and apoptosis which could be resulted in adverse effects if medication levels exceed the therapeutic range. Moreover, CMEXOs improve the antioxidant capability in both in vitro and in vivo experiments. On the other hand, the anti-angiogenesis property of CMEXOs has been reported via decrease in expression of the angiogenesis-related gene of vascular endothelial growth factor (VEGF). It is predicted that exosomal lactoferrin (LF) and kappa casein (KC) mRNAs are crucial parts of CMEXOs mediating their anticancer effects. The immunomodulatory effect of CMEXOs may be attributed to their high contents of LF and KC. According to previous works, CMEXOs are promising alternatives in developing new therapeutic models for multipile diseases. This review aims to provide an overview of the isolation, characterization and biological activities of the exosomes derived from camel milk for addressing their possible use in therapeutics.

DNA barcoding and mini-barcoding involve Cytochrome Oxidase Subunit I (COI) gene in mitochondrial genome and are used for accurate identification of species and biodiversity. The basic goal of the current study is to develop a complete reference database of fishes. It also evaluates the applicability of COI gene to identify fish at the species level with other aspects i.e., as Kimura 2 parameter (K2P) distance. The mean observed length of the sequence was ranging from 500 to 700 base pairs for fish species in DNA barcoding and from 80 to 650 base pairs for DNA mini-barcoding. This method describes the status of known to unknown samples but it also facilitates the detection of previously un-sampled species at distinct level. So, mini-barcoding is a method which focuses on the analysis of short-length DNA markers and has been demonstrated to be effective for species identification of processed food containing degraded DNA. While DNA metabarcoding refers to the automated identification of multiple species from a single bulk sample. They may contain entire organisms or a single environmental sample containing degraded DNA. Despite DNA barcoding, mini-barcoding and metabarcoding are efficient methods for species identification which are helpful in conservation and proper management of biodiversity. It aids researchers to take an account of genetic as well as evolutionary relationships by collecting their morphological, distributional and molecular data. Overall, this paper discusses DNA barcoding technology and how it has been used to various fish species, as well as its universality, adaptability, and novel approach to DNA-based species identification.

The immune system of neonate piglets differs from adult pigs in structure and competence. Although piglets are born immunocompetent, they are genuinely immunologically defenseless. To survive in the environment, piglets need passive protection provided by sow’s colostrum and milk when constantly exposed to numerous pathogens. Early assessment of piglets’ immune status may enable rapid intervention in case of detection of any deficiencies or disorders. Moreover, awareness of the piglets’ immunocompetence and the level of maternally-derived antibodies (MDA) may allow the creation of a proper vaccine schedule. Hence, extending knowledge of prenatal ontogeny of the porcine immune system, the immune status of neonate piglets’ and the immunological components of porcine colostrum is crucial. Since animal welfare has become a more critical element of animal production, new, non-invasive sampling methodologies are highly desirable for the evaluation of piglets’ immune status.

Probiotics are live microorganisms that can improve the host’s health when given in sufficient doses. Due to growing consumer demand for poultry raised without antibiotics supplementation, the usage of probiotics has been rising gradually over time in poultry. This review aimed to summarize the impacts of probiotics on poultry’s nutrient utilization, growth and carcass parameters, gut morphology, laying performance, biochemical parameters, immunity, and gut microbiota. The results revealed that probiotics enhanced the growth performance via improving digestion of protein, lipids, and carbohydrates, improving feed conversion ratio (FCR), increasing carcass and organs weights, improved liver and kidney function parameters, antioxidant effects, decreased serum lipids and cholesterol, improved poultry immunity via improving gut health and synthesis of antimicrobial peptides and prevention of microbial colonization, improved laying performance, fertility, hatchability and eggshell thickness. Therefore, probiotics have various benefits in poultry production compared to banned antibiotics. However, they are not always guaranteed to be growth promoters due to poorly understood modes of action, including their interaction with the host. So, further studies concerning their impacts on meat quality and histopathological changes are still required.

The aim of this study was to evaluate the association between gene polymorphisms (SNPs) and mastitis indicators and their relationship with milk production profitability in dairy herd. A functional analysis was also performed of five genes containing the studied SNPs and those located close by. DNA was isolated from the hair bulb of 320 dairy cows kept in three herds and SNP-microarray analysis was performed. The data on 299 cows was subjected to final statistical analysis using AI-REML method with one-trait repeatability test-day animal model and pedigree information using the DMU4 package. Five from 35 SNPs significantly associated with mastitis indicators or production traits and located within a gene or no more than 500,000 nucleotides from the gene were selected for the functional and economic analysis. A questionnaire was also developed to collect associated economic data of 219 cows from three herds, such as the value of milk production and direct costs incurred over three years; this allowed the gross margin, direct profitability index and direct costs incurred to produce one liter of milk to be determined, among others. None of the five studied SNPs were related to protein content. The rs110785912(T/A), found near CXCR4, and rs136813430(T/C), located in the TLR4 gene exon, were associated with lnSCC, while rs110455063(C/G), located near IGF-I, was associated with milk yield, fat and total solid contents. rs109421300(T/C), associated with fat/protein content ratio, as well as fat and total solid content, is located in the DGAT1 gene intron. rs41587003(A/C), located in the DLG2 gene intron, was associated with lactose content. The economic analysis revealed differences between the variants of the three tested SNPs. The T/C variant of the rs136813430(T/C) SNP was characterized by the highest gross margin, the highest direct profitability index and the lowest costs incurred to produce 1 liter of milk. The T/A variant of rs110785912(T/A) was related to low lnSC and was characterized by the highest direct profitability index. In turn, the C/C variant of the rs41587003(A/C) was related to the lowest level of lactose and the highest costs of milk production. It appears that rs136813430(T/C) may be the most promising of the tested SNPs for increasing the profitability of milk production. To our knowledge, it is the first effort to assess directly a correlation between the DNA polymorphism and economic output of a dairy enterprise.

It has been hypothesized that mutations in the ghrelin gene in pigs may play a similar role as in humans and may be associated with obesity. The aim of study was to analyse the polymorphisms of ghrelin gene and to evaluate its effect on pigs’ carcass traits. The effect of c.-93A>G, 4428T>C and g.4486C>T polymorphisms at the ghrelin gene on slaughter performance was analysed in 346 gilts represented by three breeds (Polish Landrace, Duroc, Pietrain). Animals were fattened from 30 to 100 (±2.5) kg body weight. After slaughter, the carcasses were chilled for 24 hours (4ºC), weighed and the right half-carcasses were dissected and evaluated. A number of data were obtained including: meat weight in primary cuts, weight of ham, backfat thickness and carcass yield. From breeding and production point of view, the favourable results were obtained for pigs with the GG genotype at the c.-93A>G locus, characterized by better carcass results than those with the AA genotype, e.g. higher ham weight and lower average backfat thickness. In pigs with the TT genotype at the g.4428T>C locus, we found lower mean backfat thickness than in pigs with the CC genotype.

The present study aimed to examine the effect of impaired progesterone (P₄) synthesis, observed in gilts with gonadotropin-induced estrus, on the uterine expression of receptors important for pregnancy establishment. Twenty prepubertal gilts received 750 IU PMSG and 500 IU hCG 72 h later, while 18 prepubertal gilts in the control group were observed daily for estrus behavior. Gilts were inseminated in their first estrus and slaughtered on days 10, 12, and 15 of pregnancy to collect endometrial and myometrial tissues for mRNA analysis using real-time PCR. As we previously described, gilts with PMSG/hCG-induced estrus showed decreased luteal P4 synthesis on days 10 and 12 of pregnancy. PMSG/hCG treatment did not affect P4 receptor mRNA expression in either uterine tissue. In the endometrium, a greater mRNA transcript abundance of estrogen receptors (ESR1 and ESR2), androgen receptor (AR), prostaglandin (PG) E₂ receptors (PTGER2 and PTGER4), PGF_2α receptor (PTGFR), interleukin 6 receptor (IL6R), and tumor necrosis factor α receptors (TNFRSF1A and TNFRSF1B) was detected in gilts with natural than with PMSG/hCG-induced estrus (P<0.05). In the myometrium, the mRNA expression of AR, PTGER2, and PTGFR was lower, while PGI₂ receptor (PTGIR) transcript abundance was elevated in the gilts treated with PMSG/hCG as compared with the control animals (P<0.05). In summary, a decreased luteal P₄ level during the peri-implantation period in gonadotropin-stimulated pigs affects endometrial and myometrial receptor expression, with the endometrium being more sensitive to impaired P₄ synthesis. Whether the observed changes alter uterine receptivity to local and systemic factors remains to be elucidated.

The present study investigated the impacts of multiple thunderstorm-sound exposures on growth and respiratory parameters in Nile tilapia (Oreochromis niloticus) in order to evaluate the acoustic stress response. Thunderstorm-sound exposure for 3 hours triggered respiration speed with an alarm reflex and rapid elevation of opercula beat rate (OBR) and pectoral wing rate (PWR), which increased two-fold over the control with no sound treatment, and peaked (OBR, 71.33±5.86 beat/min; PWR, 75.00±3.61 beat/min) in 10 hours after initiation of sound. Thereafter, respiration rates declined over the following days and returned to near-initial levels (45.33±4.04 beat/min OBR and 43.00±1.00 beat/min PWR) by day 3, an indication that fish recovered from thunderstorm-sound stress after 3 days of exposure. However, the same reaction course was observed each time of multiple sound exposures, repeated 20 times in a row with 4-day intervals, underlining that fish could not attune to repeated thunderstorm sound. Reduced voluntary feed intake as a result of anxiety and appetite loss was recorded in fish exposed to multiple thunderstorm sound, resulting in 50% less growth compared to those without sound treatment by the end of the 80-day experimentation. Therefore, it is advisable to monitor fish behavior during the 3-day stress period after a thunderstorm event in order to prevent waste from excess feeding, that in turn may contribute environment-friendly aquaculture for the future and sustainability of the oceans.

The antibacterial activity of zinc oxide nanoparticles (ZnO NPs) has received significant attention worldwide due to the emergence of multidrug-resistant microorganisms. Shiga toxin-producing Escherichia coli is a major foodborne pathogen that causes gastroenteritis that may be complicated by hemorrhagic colitis or hemolytic uremic syndrome. Therefore, this study aimed to evaluate the antimicrobial effect of ZnO NPs against E. coli O26 and its Shiga toxin type 2 (Stx2). Multidrug resistance phenotype was observed in E. coli O26, with co-resistance to several unrelated families of antimicrobial agents. Different concentrations of ZnO NPs nanoparticles (20 nm) were tested against different cell densities of E. coli O26 (10⁸, 10⁶ and 10⁵ CFU/ml). The minimum inhibitory concentration (MIC) value was 1 mg/ml. Minimum bactericidal concentration (MBC) was 1.5 mg/ml, 2.5 mg/ml and 3 mg/ml, respectively, depending on ZnO NPs concentrations and bacterial cell density. Results showed a significant (P≤0.05) decrease in Stx2 level in a response to ZnO NPs treatment. As detected by quantitative real-time PCR, ZnO NPs down-regulated the expression of the Stx2 gene (P≤0.05). Moreover, various concentrations of ZnO NPs considerably reduced the total protein content in E. coli O26. There was a significant reduction in protein expression with increased ZnO NPs concentration compared to the non-treated control. Scanning electron micrographs (SEM) of the treated bacteria showed severe disruptive effects on E. coli O26 with increasing ZnO NPs concentration. The results revealed a strong correlation between the antibacterial effect and ZnO NPs concentrations. ZnO NPs exert their antibacterial activities through various mechanisms and could be used as a potent antibacterial agent against E. coli O26.

The current work aimed to find substitutes for antibiotics because of the side effects of antibacterial agents and the expansion of bacterial resistance to these agents. The scope of this study was to evaluate the antibacterial activity of gold and silver nanoparticles (AuNPs and AgNPs) against selected animal pathogens (Staphylococcus aureus, Klebsiella pneumonia, Streptococcus pneumoniae, Escherichia coli, Bacillus abortus and Mycobacterium bovis). The synthesized nanoparticles were distinguished by scanning electron microscopy (SEM) analysis and tested for antibacterial activity with the broth microdilution method, well diffusion assay, and minimum bactericidal concentration procedure. Results showed that both AuNPs and AgNPs displayed good antibacterial activity against all tested bacteria. The strongest antibacterial action of AgNPS (18 mm) was against E. coli. AuNPs displayed good antibacterial activity against S. aureus and B. bovis with a suppression area of 14 mm. Therefore, it is suggested that AgNPs and AuNPs could be effectively used against animal pathogens and may contribute to reducing antibiotic resistance. However, there is a need for further research on the in vivo toxicity and mechanisms of action of AuNPs and AgNPs.

As an abundant source of antioxidants and diet flavor enhancers, the plant essential oils can have positive effects on fish growth, and resistance against environmental stressors. In this study, garden thyme (Thymus vulgaris) essential oil (TEO) was used in the diet of rainbow trout, Oncorhynchus mykiss, to evaluate its protective effect against malathion pesticide exposure. Tested fish (19.99±0.01 g) were divided into six groups (three replicates), namely: T1: control diet; T2: control diet + 0.025 mg L⁻¹ malathion; T3: control diet + 0.075 mg L⁻¹ malathion; T4: control diet + 1% TEO; T5: control diet + 0.025 mg L⁻¹ malathion + 1% TEO and T6: control diet + 0.075 mg L⁻¹ malathion + 1% TEO. After 21 days, T4 fish had the highest final body weight (FW), weight gain (WG), specific growth rate (SGR), and the lowest feed conversion ratio (FCR) among experimental treatments (P<0.05). The blood parameters including the red blood cells (RBC), white blood cell count (WBC), hematocrit (Hct), and hemoglobin (Hb) values were the highest in T4 treatment, displaying a significant difference with T1 treatment (P<0.05). Fish in the T4 groups had the highest total protein (TP) and albumin (ALB), while fish of T3 showed the lowest levels of these parameters (P<0.05) and also had the highest level of triglycerides (TRG), cholesterol (CHOL), lactate dehydrogenase (LDH), and urea (Ur). Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) enzymes recorded the lowest levels in T4 treatment, which showed a significant difference with T1 group. The catalase (CAT) and superoxide dismutase (SOD) showed the highest activities in T4 treatment, while the lowest SOD and the highest malondialdehyde (MDA) levels occurred in T3 group (P<0.05). Total immunoglobulin (total Ig) level, alternative complement (ACH₅₀) and lysozyme in the serum and skin mucus of T4 treatment of rainbow trout showed the highest activities with a significant difference from groups (P<0.05). From the results of the present study, it can be concluded that 1% of T. vulgaris as a supplement to the diet of rainbow trout can stimulate and improve the immune system of the fish. TEO can have a protective effect against unfavorable effects of malathion and improves the growth of the fish.

Nile tilapia is recognized as a suitable candidate for intensive farming and sustainability of the aquaculture industry. However, one issue limiting Nile tilapia expansion in arid and semi-arid areas is the scarcity of freshwater resources. In this study, the supplementation of synbiotics was investigated to enhance the growth performance, growth-related genes, intestinal health, and immunity of Nile tilapia reared in inland brackish groundwater. Four diets were prepared where the basal diets were mixed with the dietary mixture of probiotics and prebiotics (Synbiotic Lactic Dry^®, a blend of Saccharomyces cerevisiae, Lactobacillus acidophilus, Streptococcus faecium, and Bacillus subtilis, mannan oligosaccharides and β-1.3/1.6-D-glucan) at 0, 0.5, 1, and 2 g/kg. After eight weeks, the final weight and weight gain are linearly increasing with increasing the supplementation level of synbiotic. Markedly fish fed 0.5, 1, and 2 g/kg of synbiotic had higher final weight, weight gain, and feed intake and lower feed conversion ratio (FCR) than fish fed synbiotic free diet. The specific growth rate (SGR) was significantly higher in fish fed 1 and 2 g/kg than in fish fed 0 and 0.5 g/kg. The intestine of fish fed on synbiotic shows an increase in intestinal villi density. Further, the intestine of fish fed on synbiotic showed an increase in the length and branching intestinal villi (anterior, middle, and posterior) in a dose-dependent manner. The lysozyme and phagocytic activities were significantly different from the control, while synbiotic supplementation did not affect the phagocytic index. Interestingly, the results showed marked upregulation of ghrelin, IGF-1, and GH genes in fish fed synbiotics at 0.5, 1, and 2 g/kg. In addition, fish fed 2 g/kg had the highest expression of ghrelin, IGF-1, and GH genes. In conclusion, growing Nile tilapia in inland brackish groundwater can be achieved without negative impacts on the growth performance and health status. Supplementing synbiotics (1–2 g/kg) in Nile tilapia feeds enhanced the growth and feed performances, intestinal histomorphological features, growth-related genes, and immune response.

This study was conducted to examine the effects of powder sodium propionate (P-SP) and SP- loaded molecular imprinted polymer (MIP) nanoparticles (MIP-SP NPs) on the growth, skin mucosal immune parameters, and digestive and liver enzymes activities of African cichlid (Labidochromis lividus) fingerlings. Fish with an average weight of 500±2 mg were stocked into 12 experimental units and fed on experimental diets prepared by supplementing the basal diet (control) with MIP NPs, P-SP (5 g SP kg⁻¹ of dry diet), and MIP-SP NPs for 8 weeks. The findings demonstrated that growth indices improved in the MIP-SP NPs followed by the P-SP dietary group compared to the control groups (P<0.05). The activity of digestive enzymes of lipase, trypsin, protease, and alkaline phosphatase was higher in the fish fed SP-supplemented diets than in the controls (P<0.05). The protease and lipase activities in the MIP-SP NPs dietary group increased by 29.33% and 48.81% compared to the control, respectively (P<0.05). In addition, the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels of liver tissue decreased in the SP dietary groups, while the catalase (CAT ), superoxide dismutase (SOD), and alkaline phosphatase (ALP) levels increased compared to the control groups (P<0.05). The highest SOD and ALP levels were observed in the fish fed on the MIP-SP NPs-supplemented diet (P<0.05). Furthermore, the skin mucosal immune indices, including alternative hemolytic complement activity (ACH50), lysozyme, and total immunoglobulin (Ig) levels increased in the MIP-SP NPs and P-SP dietary groups compared to the controls (P<0.05). The findings indicated that sodium propionate encapsulated in molecularly imprinted polymer nanoparticles could enhance the efficiency of dietary SP in African cichlid fish.

Reducing the use of fishmeal (FM) in aquafeed means a significant saving in the amount of FM at the global level and reducing environmental impacts. One of the potential protein sources to replace FM in shrimp diet is the use of insects’ meal. Therefore, the present study aimed to examine the effects of replacing FM with mealworm (MW; Tenebrio molitor) on the growth, digestive enzymes activity and hepatopancreatic biochemical indices of Pacific white shrimp (Litopenaeus vannamei). Shrimp were fed for 60 days with a control diet (T0) and 4 practical diets (T15, T30, T60 and T100) where 15, 30, 60 and 100% of the FM was substituted by MW, respectively. Results showed that there were significant differences in weight gain (WG) and hepatopancreatic index (HPI) among treatments and the lowest and highest values were observed in T0 and T30, respectively. Our findings indicated a significant increase (P<0.05) in activity of glutathione peroxidase (GPX), catalase (CAT), superoxide dismutase (SOD), total nitric oxide (TNO) and total antioxidant capacity (TAC) and a meaningful decrease (P<0.05) of malondialdehyde (MDA) in hepatopancreas of L. vannamei juveniles fed diets containing MW. The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the T0 did not show a significant difference (P>0.05) with other experimental treatments. Protease and lipase indicated an increasing trend with increasing the amount of MW up to 60%. The protease activity showed a significant difference (P<0.05) between the treatments containing MW and the control treatment. These findings indicated that MW could be a feasible candidate for replacing FM in diets of the Pacific white shrimp without any detrimental effects.

Modern hybrid varieties of rye can be successfully used for feeding pigs. The aim of the research was to determine the effects of different levels (20%, 40% and 60%) of hybrid rye in diet on the growth, slaughter value, chemical composition, physical and sensory traits and the fatty acids profile in pork meat. The experiment was performed with 100 Polish Landrace pigs that were randomly allocated into 4 groups: control, without rye, and experimental with hybrid rye addition. After slaughter lean meat content estimation, carcass traits, quality characteristics and the fatty acids profile of the longissimus lumborum muscle were determined. No significant influence on the growth performance, carcass traits and the majority of physical traits, basic composition, cholesterol content and sensory features of the longissimus lumborum muscle was found. The type and levels of cereals significantly change the FA profile. More favorable values for PUFA and the ratio of n-6/n-3 acids were obtained in pigs fed with hybrid rye than with a mix of wheat with barley. In conclusion, feeding pigs with hybrid rye at the level of 20–60% in the diet results in positive effects in the fatty acids profile, without compromising daily gain, feed intake, carcass traits, physicochemical and sensory attributes of pork meat.

This current study investigated the effect of probiotics and prebiotics on the control of Salmonella spp. isolated from chicken. One hundred and eleven bacterial isolates were recovered from different chicken farms, and nine Salmonella isolates were detected with 8%. The serogroup analysis of nine Salmonella isolates showed three different groups identified as (4) S. enteritidis, (3) S. typhimurium, and (2) un-typed group. The positive-identified Salmonella was using PCR and genus-specific primer OMPCF (outer membrane protein reverse) with a target size of 204 bp. The results of Salmonella enteritidis with target size are also 304 bp and target 401 bp for Salmonella typhimurium. In addition, one hundred chicks were grouped into five groups (1, 2, 3, 4 and 5) containing 20 broiler chicks. The broilers of groups 1, 2, 3, 4 and 5 were orally inoculated with a dose of 1×10⁸ CFU nalidixic acid-resistant Salmonella enteritidis. Experimental groups were as follows: Group 1: negative control, Group 2: positive control (infected chickens), Group 3: infected chickens and treated with B. subtilis probiotic, Group 4: infected chickens and treated with levoxyl prebiotic, Group 5: infected chickens and treated with panflor antibiotic. Results showed significantly increased survival percentage against those challenged with a virulent strain of nalidixic acid probiotics and prebiotics. The oral application significantly improved the survival percentage against challenges with a virulent strain of nalidixic acid-resistant Salmonella spp. The best result of B. subtilis was 1×10⁸ which reduced the growth of the microorganism under study (S. typhimurium – S. enteritidis).

This study aims to evaluate the effects of Bacillus licheniformis and 6-phytase added alone or in combination to broiler chicken diets on the growth performance, apparent ileal digestibility coefficient (AID) of nutrients, microbial activity, and cecal bacterial communities. In total, 400 one-day-old female Ross 308 chicks were randomly allocated to 4 dietary treatments (10 replicate pens, 10 birds each). The following groups were defined: NC (negative control), basal diet without any feed additive supplementation; NC+Pro, basal diet with addition of the B. licheniformis preparation (500 g/t of diet); NC+Phy, basal diet with addition of phytase (200 g/t of diet); and NC+Pro+Phy, basal diet combined with both studied additives. B. licheniformis positively affected (P<0.05) the feed intake (FI) and feed conversion ratio (FCR) in the first 10 d of bird rearing. Moreover, phytase supplementation elevated the FCR from 21 to 35 d. In the entire experiment, an interaction between phytase and probiotic was observed only in terms of decreasing the bird FI (P=0.005) without a negative effect on the FCR (P>0.05). Furthermore, the AID of ether extract was improved by phytase supplementation. In terms of the cecal microecology, both separately administered factors promoted Lactobacillaceae in the ceca. Interactions between probiotic preparation and phytase were noted that indicated a decreased Clostridiales population and favored Ruminococcaceae proliferation. It can be concluded that for the first time in the available literature, the favorable interactions between B. licheniformis and phytase resulted in improved performance and cecal microbiota changes in broilers.

The aim of the study was to compare the effect of adding different sources of hydrolysable tannins to the sheep diet on protozoa population and carbohydrate digestion in the rumen. The study was performed in 3 Polish Lowland ewes fistulated to the rumen in a 3 × 3 Latin square design. Control sheep (CON) received (g/d): meadow hay (600), barley meal (300), soybean meal (100) and vitamin-mineral premix (20). Sheep from the experimental groups were additionally administered 12.6 g/kg DM oak bark extract (OAK) and 3.91 g/kg DM tannic acid (TAN ). The net consumption of tannins was approx. 0.4% DM for both additives. Regarding the count of protozoa, a significant interaction between diet and sampling time was documented for all ciliates (P<0.01), with a significant effect of both factors when considered separately. Experimental diets reduced the number of total protozoa and Entodinium spp. (before feeding, 2 and 4 h after feeding; P<0.01), while increasing the abundance of Isotricha spp. population (4 h after feeding; P<0.01) in the rumen. Interestingly, the count of Ophryoscolex spp. after feeding the TAN diet increased before feeding and 2 h after feeding in comparison to the CON and OAK groups, respectively, and subsequently decreased compared to the CON diet (4 and 8 h after feeding, P<0.01). A significant interaction between the diet and sampling time was observed for xylanolytic activity (P<0.01) in the rumen, with a significant effect of sampling time, which decreased its activity in CON (after feeding) and OAK sheep (2 h after feeding; P<0.01). For amylolytic activity (P<0.10), there was a trend towards a significant interaction between experimental factors, with a significant effect on both diet and sampling time. Detailed analysis showed that the TAN diet significantly reduced amylolytic activity 2 h after feeding compared to the CON group (P<0.05). In conclusion, the TAN diet significantly reduced the number of total protozoa and Entodinium spp., which consequently reduced amylolytic activity in the rumen, without any significant effect on pH and carbohydrate fermentation in the rumen.

The aim of the conducted research was to assess the impact of the botanical composition of free-range areas on the production results and selected quality parameters of the meat of fattening chickens of the Polish Yellowleg Partridge (Ż-33) breed in organic farming. Three hundred mixed-sex Ż-33 chicks were assigned to three groups: RP1 – free-range area with natural meadow vegetation, RP2 – free-range area sown with a mixture of plants containing ingredients stimulating growth, RP3 – free-range area sown with a mixture of plants rich in carotene. During the experiment, the production results (the weight of the birds, feed consumption, feed conversion ratio per g of weight gain, and mortality) were monitored. Observations were also performed on the behaviour in the free-range areas. On the 140th day of the experiment, a simplified slaughter analysis was carried out, the meat pH was measured, the colour of the muscles was also determined, as well as the water holding capacity and drip loss of the meat. In the meat samples, the content of nutrients and fatty acids was determined, and the peroxidizability index (PI) was calculated. Birds feeding in the RP2 free-range areas were characterised by higher body weight, better feed conversion, and higher dressing percentage compared to birds having access to the RP1 and RP3 free-range areas. On the other hand, the RP3 group Ż-33 chickens were characterised by a higher share of leg muscles and a tendency towards lower fat content in the carcass. It was also found that the meat of birds from the RP2 and RP3 groups was characterised by a higher pH₂₄ compared to Ż-33 chickens from the RP1 group. Access to the RP2 free-range area modified the fatty acid profile, mainly in the leg muscles, reducing the palmitic acid and MUFA content and increasing the PUFA content. It can therefore be assumed that the plant species sown in the RP2 free-range area positively influenced the production results and the quality of the meat of fattening chickens feeding on them. It is therefore appropriate to carry out further studies on the type of vegetation sown in free-range areas in order to improve the efficiency and quality of the meat of organic fattening chickens.

The study was carried out on milk from cold-blooded Sokólski mares and warm-blooded Polish Halfbred mares. Milk samples were analysed for content of solids, protein, fat, lactose, and ash; density; energy value; percentage of α-lactalbumin (α-La), β-lactoglobulin (β-Lg), serum albumin (SA), immunoglobulin (Ig), lactoferrin (Lf) and lysozyme (Lz) in the total protein; and fatty acid profile. Milk from cold-blooded mares was found to be similar in protein and fat content to that of warm-blooded mares, but had higher content of solids, including lactose and ash. It also had significantly (P≤0.01) higher content of α-La, SA and Lz, and lower content of β-Lg, Ig and Lf in comparison to milk from warm-blooded mares. Milk from Sokólski horses had a significantly (P≤0.01) higher proportion of SFA, including C10:0, C12:0, C14:0, C16:0 (P≤0.01) and C18:0 (P≤0.05), while the milk of warm-blooded mares had a significantly (P≤0.01) higher proportion of MUFA, including C16:1n-9 and C18:n-9, and PUFA, including C18:2n-6 and C18:3n3. The share of n-6 and n-3 PUFA was significantly (P≤0.01) higher in milk from warm-blooded horses, but their ratio (n-6/n-3) was lower (more favourable) in milk from cold-blooded mares. In addition, milk from warm-blooded horses had a lower (P≤0.01) share of HSFA and a higher proportion of DFA compared to cold-blooded horses, as well as lower values for AI and TI. Despite these minor differences in comparison with warmblooded mares (Polish Halfbred), milk from cold-blooded Sokólski mares was shown to be a food product of high nutritional value, which is one argument in favour of the use of this horse breed for dairy purposes.

The aims of this study were to determine the prevalence of carcass lesions in slaughtered pigs and to quantify their relationships with different animal characteristics, pre-slaughter factors, blood measurements, performance indices, carcass composition and meat quality traits. Data was recorded for 30 journeys referring to 1080 market-weight pigs that originated from 15 commercial small-scale finishing farms. Carcass lesions were visually assessed on the slaughterline in different parts of the carcass, i.e., anterior, middle and posterior, using a three-point scale. Complete blood count was investigated. The following performance indices and carcass composition traits were measured: average lifetime daily weight gain, live, hot and cold carcass weights, cooler shrinkage, dressing percentage, backfat thickness and meatiness. Meat pH and temperature were measured 45 minutes postmortem. Of the 1080 pigs slaughtered in 30 batches, 70.28% displayed some degree of lesions on the carcass (moderate – 30.00%; severe – 40.28%). The carcass lesions were the most prevalent (50.20%) in the posterior part of the pig carcass. RYR 1 genotype, live weight, loading density, lairage time, lairage density and slaughter season affected the carcass lesion prevalence. The presence of carcass lesions, irrespective of severity, was associated with alterations in blood measurements in slaughtered pigs, indicating compromised animal welfare. The presence of severe carcass lesions in slaughtered pigs was significantly associated with increased meat pH_45min, which led to the highest occurrence of dark, firm and dry pork. In contrast, there was strong evidence of association between the presence of moderate carcass lesions in slaughtered pigs and both decreased meat pH_45min and increased meat T_45min, which led to the highest occurrence of pale, soft and exudative pork among the carcass lesion groups. In conclusion, this study showed a high prevalence of carcass lesions in slaughtered pigs, whereby the risk of their occurrence was affected by both animal characteristics and pre-slaughter conditions. Also, the presence of carcass lesions in slaughtered pigs, irrespective of severity, was significantly associated with alterations in the blood measurements and pork quality.