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Zeitschriftendaten
Format
Zeitschrift
eISSN
1875-855X
Erstveröffentlichung
01 Jun 2007
Erscheinungsweise
6 Hefte pro Jahr
Sprachen
Englisch

Suche

Volumen 14 (2020): Heft 5 (October 2020)

Zeitschriftendaten
Format
Zeitschrift
eISSN
1875-855X
Erstveröffentlichung
01 Jun 2007
Erscheinungsweise
6 Hefte pro Jahr
Sprachen
Englisch

Suche

6 Artikel
access type Uneingeschränkter Zugang

Measuring signal transduction and transcription molecules for clinical use

Online veröffentlicht: 31 Oct 2020
Seitenbereich: 175 - 176

Zusammenfassung

access type Uneingeschränkter Zugang

Advances in automated techniques to identify Acinetobacter calcoaceticus–Acinetobacter baumannii complex

Online veröffentlicht: 31 Oct 2020
Seitenbereich: 177 - 186

Zusammenfassung

Abstract

Acinetobacter species, particularly those within Acinetobacter calcoaceticus–A. baumannii complex (ACB complex), have emerged as clinically relevant pathogens in hospital environments worldwide. Early and quick detection and identification of Acinetobacter infections is challenging, and traditional culture and biochemical methods may not achieve adequate levels of speciation. Moreover, currently available techniques to identify and differentiate closely related Acinetobacter species are insufficient. The objective of this review is to recapitulate the current evolution in phenotypic and automated techniques used to identify the ACB complex. Compared with other automated or semiautomated systems of bacterial identification, matrix-assisted laser desorption–ionization time-of-flight mass spectrometry (MALDI-TOF MS) demonstrates a high level of Acinetobacter species identification and discrimination, including newly discovered species A. seifertii and A. dijkshoorniae.

Schlüsselwörter

  • automation, laboratory
  • bacterial typing techniques
  • bacteriological techniques
  • spectrometry, mass, matrix-assisted laser desorption-ionization
access type Uneingeschränkter Zugang

β-Catenin immunocytochemical reactivity in cervicovaginal smears during regular menstrual cycles

Online veröffentlicht: 31 Oct 2020
Seitenbereich: 187 - 194

Zusammenfassung

AbstractBackground

β-Catenin mediates cellular adhesion and the Wnt/β-catenin signaling mechanism, thereby controlling cell proliferation and differentiation. Studies of endometrial tissue suggest that there are differences in β-catenin expression during the course of regular menstrual cycles. However, differences in expression in squamous epithelial cells between the proliferative and secretory phases have hitherto remained unknown.

Objectives

To localize β-catenin in squamous epithelial cells in cervicovaginal smears during the course of regular menstrual cycles.

Methods

In this observational study, smears were taken from women (n = 102) with various gynecological complaints. Squamous epithelial cells were stained using a Papanicolaou method to evaluate their cytology and any infection. An anti-β-catenin antibody was used to localize immunoreactivity in the cell membrane, cytoplasm, and/or nucleus.

Results

Women with a regular menstrual cycle (n = 62) were divided into 2 groups: those in a proliferative phase (26/62, 42%) and those in a secretory phase (36/62, 58%). Cytoplasmic and nuclear β-catenin immunoreactivity was observed prominently in the proliferative phase (19/26, 73%), whereas low-level β-catenin immunoreactivity was seen in the secretory phase (9/36, 25%). Compared with the secretory phase, the mean H-scores for β-catenin immunoreactivity in the proliferative phase were significantly increased in the membrane (P = 0.039), the cytoplasm (P < 0.001), and the nucleus (P = 0.033). By contrast, β-catenin immunoreactivity was reduced from parabasal to superficial cells in both the proliferative and secretory phases.

Conclusions

Cytoplasmic and/or nuclear β-catenin immunoreactivity may indicate that the activity of the Wnt/β-catenin signaling pathway is cycle dependent.

Schlüsselwörter

  • β-catenin
  • menstrual cycle
  • Papanicolaou test
  • vaginal smears
  • Wnt signaling pathway
access type Uneingeschränkter Zugang

Diagnostic performance of renal ultrasonography in detecting chronic kidney disease of various severity

Online veröffentlicht: 31 Oct 2020
Seitenbereich: 195 - 202

Zusammenfassung

AbstractBackground

Association between early diagnosis of chronic kidney disease (CKD) and low morbidity and mortality rate has been proven. Thus, tools for early CKD diagnosis are vital. Ultrasonography has been widely used to diagnose and monitor the progression of CKD.

Objectives

To determine the performance of selected renal ultrasonographic parameters for the diagnosis of early CKD.

Methods

In a cohort of patients diagnosed with CKD (n = 100), diagnostic performance of ultrasonographically measured renal length (RL), renal cortical thickness (RCT), and parenchymal thickness (PT) was determined using receiver operating curve analysis; correlation of each parameter with the associated comorbidities and serum creatinine (Scr) levels was also determined. Severity of CKD was graded with estimated glomerular filtration rates (eGFR).

Results

Of all patient participants, 85 had severity grades 2 or 3. Mean (standard deviation) Scr was 1.88 (0.60) mg/dL; eGFR was 43.3 (11.85) mL/min/1.73 m2. RL was 9.01 (0.83) cm, PT was 1.32 (0.22) cm, and RCT was 6.0 (0.10) mm. PT and RCT were positively correlated with eGFR (P = 0.01 and 0.002, respectively). Early CKD was better predicted by PT (area under the curve (AUC) 0.735; 82% sensitivity; 30% specificity; 68% positive predictive value (PPV)) and RCT (AUC 0.741; 82% sensitivity; 48% specificity; 51% PPV); severe CKD was better predicted by RL (AUC 0.809; 67% sensitivity; 26% specificity, 45% PPV; 13% negative predictive value).

Conclusion

Index ultrasonic parameters show a diagnostic role in different stages of CKD. The index ultrasound and biochemical parameters showed a complementary role in predicting renal dysfunction.

Schlüsselwörter

  • creatinine
  • kidney cortex
  • renal insufficiency, chronic
  • renal parenchyma
  • ultrasonography
access type Uneingeschränkter Zugang

Nuclear protein in testis midline carcinoma in a Turkish boy: a case report

Online veröffentlicht: 31 Oct 2020
Seitenbereich: 203 - 208

Zusammenfassung

AbstractBackground

Nuclear protein in testis (NUT) midline carcinoma (NMC) is an undifferentiated carcinoma, usually localized to the midline and presenting a translocation in the gene for bromodomain containing protein 4. Here, we report a rare case of NMC in an 8-year-old Turkish boy.

Case report

There were masses in the lung, liver, and iliac wing representing metastases. Abdominal lymph node sampling revealed epithelial tumor infiltration with cellular pleomorphism. Immunohistochemistry was strongly positive for cytokeratin and epithelial membrane antigen protein. Because of undifferentiated carcinoma morphology, the tumor was considered to be a NMC. Immunoreactivity with antibodies to NUT and the presence of NUT clarified by fluorescence in situ hybridization (FISH) supported the diagnosis. Despite initial response to chemotherapy, the patient died 7 months after the diagnosis.

Conclusions

Immunoreactivity for NUT antibodies along with a dual-color FISH and karyotype analysis was suggestive for diagnosis of NMC. In differential diagnosis of undifferentiated carcinomas that occur particularly at midline localization, NMC should be considered.

Schlüsselwörter

  • carcinoma
  • immunohistochemistry
  • in situ hybridization, fluorescence
  • nuclear protein in testis, human
  • Turkey
access type Uneingeschränkter Zugang

False-positive dengue IgM test result in a patient with systemic lupus erythematosus: a case report

Online veröffentlicht: 31 Oct 2020
Seitenbereich: 209 - 213

Zusammenfassung

Abstract

Dengue virus infection most commonly has mild-to-moderate nonspecific clinical presentations that overlap with other diseases. Dengue-specific tests are commonly used for those patients with acute febrile illness in dengue-endemic areas. There is one study in vitro that showed a false-positive dengue-immunoglobulin M (dengue IgM) test for blood from a patient with systemic lupus erythematosus (SLE). Here, we demonstrated a false-positive dengue IgM test in a patient with SLE. The patient had fever, cytopenia, and a skin rash, but her clinical variables more closely matched with the criteria for SLE than the dengue infection. Vasculitis-like-lesions supported prednisolone administration and her clinical symptoms improved. This case highlights that some patients with SLE can be misdiagnosed as having a viral infection. These two diseases have similar clinical findings, such as acute febrile illness, but they are different in terms of their treatments and disease prognosis.

Schlüsselwörter

  • connective tissue diseases
  • dengue virus
  • false positive reactions
  • immunoglobulin M
  • lupus erythematosus, systemic
6 Artikel
access type Uneingeschränkter Zugang

Measuring signal transduction and transcription molecules for clinical use

Online veröffentlicht: 31 Oct 2020
Seitenbereich: 175 - 176

Zusammenfassung

access type Uneingeschränkter Zugang

Advances in automated techniques to identify Acinetobacter calcoaceticus–Acinetobacter baumannii complex

Online veröffentlicht: 31 Oct 2020
Seitenbereich: 177 - 186

Zusammenfassung

Abstract

Acinetobacter species, particularly those within Acinetobacter calcoaceticus–A. baumannii complex (ACB complex), have emerged as clinically relevant pathogens in hospital environments worldwide. Early and quick detection and identification of Acinetobacter infections is challenging, and traditional culture and biochemical methods may not achieve adequate levels of speciation. Moreover, currently available techniques to identify and differentiate closely related Acinetobacter species are insufficient. The objective of this review is to recapitulate the current evolution in phenotypic and automated techniques used to identify the ACB complex. Compared with other automated or semiautomated systems of bacterial identification, matrix-assisted laser desorption–ionization time-of-flight mass spectrometry (MALDI-TOF MS) demonstrates a high level of Acinetobacter species identification and discrimination, including newly discovered species A. seifertii and A. dijkshoorniae.

Schlüsselwörter

  • automation, laboratory
  • bacterial typing techniques
  • bacteriological techniques
  • spectrometry, mass, matrix-assisted laser desorption-ionization
access type Uneingeschränkter Zugang

β-Catenin immunocytochemical reactivity in cervicovaginal smears during regular menstrual cycles

Online veröffentlicht: 31 Oct 2020
Seitenbereich: 187 - 194

Zusammenfassung

AbstractBackground

β-Catenin mediates cellular adhesion and the Wnt/β-catenin signaling mechanism, thereby controlling cell proliferation and differentiation. Studies of endometrial tissue suggest that there are differences in β-catenin expression during the course of regular menstrual cycles. However, differences in expression in squamous epithelial cells between the proliferative and secretory phases have hitherto remained unknown.

Objectives

To localize β-catenin in squamous epithelial cells in cervicovaginal smears during the course of regular menstrual cycles.

Methods

In this observational study, smears were taken from women (n = 102) with various gynecological complaints. Squamous epithelial cells were stained using a Papanicolaou method to evaluate their cytology and any infection. An anti-β-catenin antibody was used to localize immunoreactivity in the cell membrane, cytoplasm, and/or nucleus.

Results

Women with a regular menstrual cycle (n = 62) were divided into 2 groups: those in a proliferative phase (26/62, 42%) and those in a secretory phase (36/62, 58%). Cytoplasmic and nuclear β-catenin immunoreactivity was observed prominently in the proliferative phase (19/26, 73%), whereas low-level β-catenin immunoreactivity was seen in the secretory phase (9/36, 25%). Compared with the secretory phase, the mean H-scores for β-catenin immunoreactivity in the proliferative phase were significantly increased in the membrane (P = 0.039), the cytoplasm (P < 0.001), and the nucleus (P = 0.033). By contrast, β-catenin immunoreactivity was reduced from parabasal to superficial cells in both the proliferative and secretory phases.

Conclusions

Cytoplasmic and/or nuclear β-catenin immunoreactivity may indicate that the activity of the Wnt/β-catenin signaling pathway is cycle dependent.

Schlüsselwörter

  • β-catenin
  • menstrual cycle
  • Papanicolaou test
  • vaginal smears
  • Wnt signaling pathway
access type Uneingeschränkter Zugang

Diagnostic performance of renal ultrasonography in detecting chronic kidney disease of various severity

Online veröffentlicht: 31 Oct 2020
Seitenbereich: 195 - 202

Zusammenfassung

AbstractBackground

Association between early diagnosis of chronic kidney disease (CKD) and low morbidity and mortality rate has been proven. Thus, tools for early CKD diagnosis are vital. Ultrasonography has been widely used to diagnose and monitor the progression of CKD.

Objectives

To determine the performance of selected renal ultrasonographic parameters for the diagnosis of early CKD.

Methods

In a cohort of patients diagnosed with CKD (n = 100), diagnostic performance of ultrasonographically measured renal length (RL), renal cortical thickness (RCT), and parenchymal thickness (PT) was determined using receiver operating curve analysis; correlation of each parameter with the associated comorbidities and serum creatinine (Scr) levels was also determined. Severity of CKD was graded with estimated glomerular filtration rates (eGFR).

Results

Of all patient participants, 85 had severity grades 2 or 3. Mean (standard deviation) Scr was 1.88 (0.60) mg/dL; eGFR was 43.3 (11.85) mL/min/1.73 m2. RL was 9.01 (0.83) cm, PT was 1.32 (0.22) cm, and RCT was 6.0 (0.10) mm. PT and RCT were positively correlated with eGFR (P = 0.01 and 0.002, respectively). Early CKD was better predicted by PT (area under the curve (AUC) 0.735; 82% sensitivity; 30% specificity; 68% positive predictive value (PPV)) and RCT (AUC 0.741; 82% sensitivity; 48% specificity; 51% PPV); severe CKD was better predicted by RL (AUC 0.809; 67% sensitivity; 26% specificity, 45% PPV; 13% negative predictive value).

Conclusion

Index ultrasonic parameters show a diagnostic role in different stages of CKD. The index ultrasound and biochemical parameters showed a complementary role in predicting renal dysfunction.

Schlüsselwörter

  • creatinine
  • kidney cortex
  • renal insufficiency, chronic
  • renal parenchyma
  • ultrasonography
access type Uneingeschränkter Zugang

Nuclear protein in testis midline carcinoma in a Turkish boy: a case report

Online veröffentlicht: 31 Oct 2020
Seitenbereich: 203 - 208

Zusammenfassung

AbstractBackground

Nuclear protein in testis (NUT) midline carcinoma (NMC) is an undifferentiated carcinoma, usually localized to the midline and presenting a translocation in the gene for bromodomain containing protein 4. Here, we report a rare case of NMC in an 8-year-old Turkish boy.

Case report

There were masses in the lung, liver, and iliac wing representing metastases. Abdominal lymph node sampling revealed epithelial tumor infiltration with cellular pleomorphism. Immunohistochemistry was strongly positive for cytokeratin and epithelial membrane antigen protein. Because of undifferentiated carcinoma morphology, the tumor was considered to be a NMC. Immunoreactivity with antibodies to NUT and the presence of NUT clarified by fluorescence in situ hybridization (FISH) supported the diagnosis. Despite initial response to chemotherapy, the patient died 7 months after the diagnosis.

Conclusions

Immunoreactivity for NUT antibodies along with a dual-color FISH and karyotype analysis was suggestive for diagnosis of NMC. In differential diagnosis of undifferentiated carcinomas that occur particularly at midline localization, NMC should be considered.

Schlüsselwörter

  • carcinoma
  • immunohistochemistry
  • in situ hybridization, fluorescence
  • nuclear protein in testis, human
  • Turkey
access type Uneingeschränkter Zugang

False-positive dengue IgM test result in a patient with systemic lupus erythematosus: a case report

Online veröffentlicht: 31 Oct 2020
Seitenbereich: 209 - 213

Zusammenfassung

Abstract

Dengue virus infection most commonly has mild-to-moderate nonspecific clinical presentations that overlap with other diseases. Dengue-specific tests are commonly used for those patients with acute febrile illness in dengue-endemic areas. There is one study in vitro that showed a false-positive dengue-immunoglobulin M (dengue IgM) test for blood from a patient with systemic lupus erythematosus (SLE). Here, we demonstrated a false-positive dengue IgM test in a patient with SLE. The patient had fever, cytopenia, and a skin rash, but her clinical variables more closely matched with the criteria for SLE than the dengue infection. Vasculitis-like-lesions supported prednisolone administration and her clinical symptoms improved. This case highlights that some patients with SLE can be misdiagnosed as having a viral infection. These two diseases have similar clinical findings, such as acute febrile illness, but they are different in terms of their treatments and disease prognosis.

Schlüsselwörter

  • connective tissue diseases
  • dengue virus
  • false positive reactions
  • immunoglobulin M
  • lupus erythematosus, systemic

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