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Zeitschriftendaten
Format
Zeitschrift
eISSN
1875-855X
Erstveröffentlichung
01 Jun 2007
Erscheinungsweise
6 Hefte pro Jahr
Sprachen
Englisch

Suche

Volumen 13 (2019): Heft 4 (August 2019)

Zeitschriftendaten
Format
Zeitschrift
eISSN
1875-855X
Erstveröffentlichung
01 Jun 2007
Erscheinungsweise
6 Hefte pro Jahr
Sprachen
Englisch

Suche

6 Artikel

Editorial

Uneingeschränkter Zugang

The role of nanoparticles for biomedical application

Online veröffentlicht: 31 Mar 2020
Seitenbereich: 121 - 122

Zusammenfassung

Original article

Uneingeschränkter Zugang

Differential migration-related gene expression and altered cytokine secretion in response to serum starvation in cultured MDA-MB-231 cells

Online veröffentlicht: 31 Mar 2020
Seitenbereich: 123 - 129

Zusammenfassung

AbstractBackground

Serum starvation is mostly considered as a standard preparatory method in many cellular and molecular experiments. However, recent studies give some evidence that serum starvation is a major event that triggers various cell responses and has therefore great potential to change and interfere with the experimental results. In this study, the behavior of breast cancer cells in serum-starved condition was examined.

Objective

To focus on the role of serum starvation on cell migration and also the possible changes in the expression and secretion of genes and cytokines mostly involved in migration and chemotaxis of breast cancer cells.

Methods

MDA-MB-231 cells were cultured under serum-starved condition. Transwell migration assay was performed to evaluate the effect of serum starvation on cell migration after 24, 48, and 72 h. The transcriptional expression of migration-related genes was evaluated using real-time polymerase chain reaction. The cytokine secretion was also analyzed using enzyme-linked immunosorbent assay.

Results

Serum starvation suppressed cell migration in breast cancer cells. Additionally, the gene expression of markers involved in migration including β-catenin, twist, zinc finger E-box binding homeobox 1, vimentin, fibronectin, intercellular adhesion molecule 1, and vascular endothelial growth factor were downregulated. Moreover, cytokines of transforming growth factor, beta 1, matrix metallopeptidase 9, interleukin 8, and nitric oxide were differentially secreted.

Conclusions

Serum deprivation causes significant changes in cancer cell migration and also the expression of migration-related genes and cytokines, special care needs to be taken when this practice is used as preparatory method especially in migration and chemotaxis experiments on cancer cells.

Schlüsselwörter

  • breast neoplasms
  • cell movement
  • epithelial mesenchymal transition
Uneingeschränkter Zugang

(2E)-2-Benzylidene-4,7-dimethyl-2,3-dihydro-1H-inden-1-one (MLT-401), a novel arylidene indanone derivative, scavenges free radicals and exhibits antiproliferative activity of Jurkat cells

Online veröffentlicht: 31 Mar 2020
Seitenbereich: 131 - 139

Zusammenfassung

AbstractBackground

The arylidene indanone scaffold has contributed many lead molecules in chemotherapeutic anticancer agent research.

Objectives

To determine the oxidant-scavenging activities and antiproliferative activity of (2E)-2-benzylidene-4,7-dimethyl-2,3-dihydro-1H-inden-1-one (MLT-401), an arylidene indanone derivative.

Methods

Jurkat cells, primary lymphocytes, and Vero cells were treated with MLT-401. Antioxidant properties of MLT-401 were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH)-based, 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS)-based, and ferric-reducing antioxidant potential (FRAP) assays. Inhibition of cell proliferation was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-based assay. Nuclear status was determined using a DNA fragmentation assay, and cell cycle stage was analyzed by flow cytometry. Mitochondrial membrane enzyme activities were measured using colorimetric methods.

Results

The antioxidant assays gave MLT-401 half maximal inhibitory concentration (IC50) values of 1611 nM (DPPH-based assay), 2115 nM (ABTS-based assay), and 1586 nM (FRAP assay). MLT-401 inhibited proliferation of Jurkat cells with a concentration for 50% of maximal inhibition of cell proliferation (GI50) of 341.5 nM, being 12- and 9-fold less than GI50 concentrations for normal lymphocytes and Vero cells, respectively. MLT-401 caused nuclear fragmentation and DNA laddering as seen by electrophoresis. Jurkat cells showed a time-dependent accumulation of sub G0/G1 cells after MLT-401 treatment. Mitochondrial membrane-bound Na+/K+ ATPase, Ca2+ ATPase, and Mg2+ ATPase activities were inhibited by MLT-401 in a dose-dependent manner.

Conclusion

MLT-401 possesses significant antiproliferative activity and scavenges free radicals released through mitochondrial membrane damage in a Jurkat cell line model of cancer cells. Further investigation of MLT-401 as a chemotherapeutic anticancer agent and development of other arylidene indanone analogues are warranted. A detailed elucidation of mechanistic pathways is required for further development.

Schlüsselwörter

  • antioxidants
  • antitumor drugs
  • antitumor drug screens
  • ATPases
  • indanones

Brief communication (original)

Uneingeschränkter Zugang

Residents as teachers: optimizing the benefit of a difficult airway management simulation session

Online veröffentlicht: 31 Mar 2020
Seitenbereich: 141 - 147

Zusammenfassung

AbstractBackground

Simulation is widely used in airway management training.

Objectives

To show that assigning anesthesia residents’ simulation educator roles improved cognitive learning outcomes.

Methods

Postgraduate second- and third-year (PGY-2 and PGY-3) anesthesia residents were randomly assigned to three groups: a teacher group (T), a hot-seat (active participant) group (H), and an observer group (O). After a train-the-trainer session, the T group prepared simulation scenarios for difficult airway management and then conducted the simulation sessions and post-session debriefing. The H group participated in the scenarios, and the O group observed the sessions. All participants attended the post-session debriefing. Evaluation was conducted at pretest, immediate posttest, and 3 months (retention test). Score differentiation and average normalized gain were calculated. Participants completed a post-simulation class survey.

Results

Participants were 49 residents (PGY-2 = 24, PGY-3 = 25). The T group had the highest posttest score (17.06 ± 1.23); this score significantly differed from the O group (14.75 ± 2.57, P = 0.003) but not the H group (15.64 ± 1.54, P = 0.103). The average normalized gain was significantly higher in the T group than in the H and O groups (0.51 ± 0.22, 0.18 ± 0.32, and 0.17 ± 0.47, respectively; P = 0.012). Participants retained knowledge at 3 months after the session, with no significant differences among the groups. Most participants (45%) preferred to be active scenario participants, and 20% preferred to teach. Overall satisfaction was high in all groups.

Conclusion

This study showed that a teaching role can be effectively applied for residents in simulation-based education on difficult airway management to support better learning outcomes.

Schlüsselwörter

  • anesthesia resident training
  • difficult airway
  • peer teaching
  • retention knowledge
  • role in simulation

Technical report

Uneingeschränkter Zugang

A new methodology for simultaneous comparison and optimization between nanoparticles and their drug conjugates against various multidrug-resistant bacterial strains

Online veröffentlicht: 31 Mar 2020
Seitenbereich: 149 - 162

Zusammenfassung

AbstractBackground

Multidrug-resistant bacteria are becoming more hazardous day by day for human health all over the world, and the scientific community is trying hard to resolve this issue by various approaches. One of the very common approaches is to bind drugs to nanoparticles and study enhanced antibacterial properties.

Objective

To compare simultaneously different types of nanoparticles, their concentration, bacterial strains and their incubation time intervals for each of the selected drug combination.

Methods

We have selected the most commonly used gold and silver nanoparticles and few examples from fluoroquinolone antibiotics to make their conjugates and study their efficacy against multidrug-resistant E. coli and S. aureus strains simultaneously, at different incubation time intervals and different concentration of nanoparticles.

Results

Gold nanoparticle hybrids do not show any significant effect. Silver nanoparticle hybrids show far better results, even at extremely low concentrations.

Conclusions

This unique and simple approach allows us to know the exact time intervals and concentration required for each nanoparticle combination to control the growth for any specific strain. This approach can be extended to any set of nanoparticles, drugs and bacterial strains for comparative purposes.

Schlüsselwörter

  • anti-bacterial agents
  • colloidal silver
  • drug resistance
  • fluoroquinolones
  • gold colloid
  • metal nanoparticles

Clinical report

Uneingeschränkter Zugang

Twin-to-twin transfusion syndrome as a cause of neonatal acute liver failure

Online veröffentlicht: 31 Mar 2020
Seitenbereich: 163 - 170

Zusammenfassung

AbstractBackground

Acute liver failure (ALF) is a rare condition during neonatal period.

Objective

To report a case of recipient twin with fulminant ALF secondary to hydrops fetalis caused by twin-to-twin transfusion syndrome (TTTS).

Method

The patient was admitted to the neonatal intensive care unit (NICU) for respiratory failure requiring mechanical ventilation and fulminant ALF with prolonged international normalized ratio (INR) and elevated liver enzymes with highest aspartate aminotransferase of 4,580 U/L.

Results

Laboratory investigation for secondary causes of liver failure was not revealing. Her liver enzymes and coagulation levels were dramatically normalized as the clinical symptoms of hypervolemia improved within 1 week.

Conclusion

TTTS can be a possible cause of neonatal ALF. Early detection with proper management of TTTS is important to avoid adverse outcomes. However, pathogenesis of hepatic dysfunction in TTTS is rarely described, and further studies are needed to help understanding the correlation between liver diseases and TTTS.

Schlüsselwörter

  • acute/therapy
  • fetofetal transfusion
  • hydrops fetalis
  • liver failure
  • newborn
6 Artikel

Editorial

Uneingeschränkter Zugang

The role of nanoparticles for biomedical application

Online veröffentlicht: 31 Mar 2020
Seitenbereich: 121 - 122

Zusammenfassung

Original article

Uneingeschränkter Zugang

Differential migration-related gene expression and altered cytokine secretion in response to serum starvation in cultured MDA-MB-231 cells

Online veröffentlicht: 31 Mar 2020
Seitenbereich: 123 - 129

Zusammenfassung

AbstractBackground

Serum starvation is mostly considered as a standard preparatory method in many cellular and molecular experiments. However, recent studies give some evidence that serum starvation is a major event that triggers various cell responses and has therefore great potential to change and interfere with the experimental results. In this study, the behavior of breast cancer cells in serum-starved condition was examined.

Objective

To focus on the role of serum starvation on cell migration and also the possible changes in the expression and secretion of genes and cytokines mostly involved in migration and chemotaxis of breast cancer cells.

Methods

MDA-MB-231 cells were cultured under serum-starved condition. Transwell migration assay was performed to evaluate the effect of serum starvation on cell migration after 24, 48, and 72 h. The transcriptional expression of migration-related genes was evaluated using real-time polymerase chain reaction. The cytokine secretion was also analyzed using enzyme-linked immunosorbent assay.

Results

Serum starvation suppressed cell migration in breast cancer cells. Additionally, the gene expression of markers involved in migration including β-catenin, twist, zinc finger E-box binding homeobox 1, vimentin, fibronectin, intercellular adhesion molecule 1, and vascular endothelial growth factor were downregulated. Moreover, cytokines of transforming growth factor, beta 1, matrix metallopeptidase 9, interleukin 8, and nitric oxide were differentially secreted.

Conclusions

Serum deprivation causes significant changes in cancer cell migration and also the expression of migration-related genes and cytokines, special care needs to be taken when this practice is used as preparatory method especially in migration and chemotaxis experiments on cancer cells.

Schlüsselwörter

  • breast neoplasms
  • cell movement
  • epithelial mesenchymal transition
Uneingeschränkter Zugang

(2E)-2-Benzylidene-4,7-dimethyl-2,3-dihydro-1H-inden-1-one (MLT-401), a novel arylidene indanone derivative, scavenges free radicals and exhibits antiproliferative activity of Jurkat cells

Online veröffentlicht: 31 Mar 2020
Seitenbereich: 131 - 139

Zusammenfassung

AbstractBackground

The arylidene indanone scaffold has contributed many lead molecules in chemotherapeutic anticancer agent research.

Objectives

To determine the oxidant-scavenging activities and antiproliferative activity of (2E)-2-benzylidene-4,7-dimethyl-2,3-dihydro-1H-inden-1-one (MLT-401), an arylidene indanone derivative.

Methods

Jurkat cells, primary lymphocytes, and Vero cells were treated with MLT-401. Antioxidant properties of MLT-401 were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH)-based, 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS)-based, and ferric-reducing antioxidant potential (FRAP) assays. Inhibition of cell proliferation was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-based assay. Nuclear status was determined using a DNA fragmentation assay, and cell cycle stage was analyzed by flow cytometry. Mitochondrial membrane enzyme activities were measured using colorimetric methods.

Results

The antioxidant assays gave MLT-401 half maximal inhibitory concentration (IC50) values of 1611 nM (DPPH-based assay), 2115 nM (ABTS-based assay), and 1586 nM (FRAP assay). MLT-401 inhibited proliferation of Jurkat cells with a concentration for 50% of maximal inhibition of cell proliferation (GI50) of 341.5 nM, being 12- and 9-fold less than GI50 concentrations for normal lymphocytes and Vero cells, respectively. MLT-401 caused nuclear fragmentation and DNA laddering as seen by electrophoresis. Jurkat cells showed a time-dependent accumulation of sub G0/G1 cells after MLT-401 treatment. Mitochondrial membrane-bound Na+/K+ ATPase, Ca2+ ATPase, and Mg2+ ATPase activities were inhibited by MLT-401 in a dose-dependent manner.

Conclusion

MLT-401 possesses significant antiproliferative activity and scavenges free radicals released through mitochondrial membrane damage in a Jurkat cell line model of cancer cells. Further investigation of MLT-401 as a chemotherapeutic anticancer agent and development of other arylidene indanone analogues are warranted. A detailed elucidation of mechanistic pathways is required for further development.

Schlüsselwörter

  • antioxidants
  • antitumor drugs
  • antitumor drug screens
  • ATPases
  • indanones

Brief communication (original)

Uneingeschränkter Zugang

Residents as teachers: optimizing the benefit of a difficult airway management simulation session

Online veröffentlicht: 31 Mar 2020
Seitenbereich: 141 - 147

Zusammenfassung

AbstractBackground

Simulation is widely used in airway management training.

Objectives

To show that assigning anesthesia residents’ simulation educator roles improved cognitive learning outcomes.

Methods

Postgraduate second- and third-year (PGY-2 and PGY-3) anesthesia residents were randomly assigned to three groups: a teacher group (T), a hot-seat (active participant) group (H), and an observer group (O). After a train-the-trainer session, the T group prepared simulation scenarios for difficult airway management and then conducted the simulation sessions and post-session debriefing. The H group participated in the scenarios, and the O group observed the sessions. All participants attended the post-session debriefing. Evaluation was conducted at pretest, immediate posttest, and 3 months (retention test). Score differentiation and average normalized gain were calculated. Participants completed a post-simulation class survey.

Results

Participants were 49 residents (PGY-2 = 24, PGY-3 = 25). The T group had the highest posttest score (17.06 ± 1.23); this score significantly differed from the O group (14.75 ± 2.57, P = 0.003) but not the H group (15.64 ± 1.54, P = 0.103). The average normalized gain was significantly higher in the T group than in the H and O groups (0.51 ± 0.22, 0.18 ± 0.32, and 0.17 ± 0.47, respectively; P = 0.012). Participants retained knowledge at 3 months after the session, with no significant differences among the groups. Most participants (45%) preferred to be active scenario participants, and 20% preferred to teach. Overall satisfaction was high in all groups.

Conclusion

This study showed that a teaching role can be effectively applied for residents in simulation-based education on difficult airway management to support better learning outcomes.

Schlüsselwörter

  • anesthesia resident training
  • difficult airway
  • peer teaching
  • retention knowledge
  • role in simulation

Technical report

Uneingeschränkter Zugang

A new methodology for simultaneous comparison and optimization between nanoparticles and their drug conjugates against various multidrug-resistant bacterial strains

Online veröffentlicht: 31 Mar 2020
Seitenbereich: 149 - 162

Zusammenfassung

AbstractBackground

Multidrug-resistant bacteria are becoming more hazardous day by day for human health all over the world, and the scientific community is trying hard to resolve this issue by various approaches. One of the very common approaches is to bind drugs to nanoparticles and study enhanced antibacterial properties.

Objective

To compare simultaneously different types of nanoparticles, their concentration, bacterial strains and their incubation time intervals for each of the selected drug combination.

Methods

We have selected the most commonly used gold and silver nanoparticles and few examples from fluoroquinolone antibiotics to make their conjugates and study their efficacy against multidrug-resistant E. coli and S. aureus strains simultaneously, at different incubation time intervals and different concentration of nanoparticles.

Results

Gold nanoparticle hybrids do not show any significant effect. Silver nanoparticle hybrids show far better results, even at extremely low concentrations.

Conclusions

This unique and simple approach allows us to know the exact time intervals and concentration required for each nanoparticle combination to control the growth for any specific strain. This approach can be extended to any set of nanoparticles, drugs and bacterial strains for comparative purposes.

Schlüsselwörter

  • anti-bacterial agents
  • colloidal silver
  • drug resistance
  • fluoroquinolones
  • gold colloid
  • metal nanoparticles

Clinical report

Uneingeschränkter Zugang

Twin-to-twin transfusion syndrome as a cause of neonatal acute liver failure

Online veröffentlicht: 31 Mar 2020
Seitenbereich: 163 - 170

Zusammenfassung

AbstractBackground

Acute liver failure (ALF) is a rare condition during neonatal period.

Objective

To report a case of recipient twin with fulminant ALF secondary to hydrops fetalis caused by twin-to-twin transfusion syndrome (TTTS).

Method

The patient was admitted to the neonatal intensive care unit (NICU) for respiratory failure requiring mechanical ventilation and fulminant ALF with prolonged international normalized ratio (INR) and elevated liver enzymes with highest aspartate aminotransferase of 4,580 U/L.

Results

Laboratory investigation for secondary causes of liver failure was not revealing. Her liver enzymes and coagulation levels were dramatically normalized as the clinical symptoms of hypervolemia improved within 1 week.

Conclusion

TTTS can be a possible cause of neonatal ALF. Early detection with proper management of TTTS is important to avoid adverse outcomes. However, pathogenesis of hepatic dysfunction in TTTS is rarely described, and further studies are needed to help understanding the correlation between liver diseases and TTTS.

Schlüsselwörter

  • acute/therapy
  • fetofetal transfusion
  • hydrops fetalis
  • liver failure
  • newborn

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