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Volume 54 (2017): Edizione 4 (December 2017)
Special Edizione: This issue is dedicated to the memory of Milan Ryboš, DVM, D.Sc.

Volume 54 (2017): Edizione 3 (September 2017)

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Volume 53 (2016): Edizione 4 (December 2016)

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Volume 52 (2015): Edizione 4 (December 2015)

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Volume 51 (2014): Edizione 4 (December 2014)

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Volume 51 (2014): Edizione 2 (June 2014)

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Volume 49 (2012): Edizione 4 (December 2012)

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Volume 48 (2011): Edizione 4 (December 2011)

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Volume 47 (2010): Edizione 4 (December 2010)

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Volume 46 (2009): Edizione 4 (December 2009)

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Volume 45 (2008): Edizione 4 (December 2008)

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Volume 43 (2006): Edizione 1 (March 2006)

Dettagli della rivista
Formato
Rivista
eISSN
1336-9083
ISSN
0440-6605
Pubblicato per la prima volta
22 Apr 2006
Periodo di pubblicazione
4 volte all'anno
Lingue
Inglese

Cerca

Volume 51 (2014): Edizione 2 (June 2014)

Dettagli della rivista
Formato
Rivista
eISSN
1336-9083
ISSN
0440-6605
Pubblicato per la prima volta
22 Apr 2006
Periodo di pubblicazione
4 volte all'anno
Lingue
Inglese

Cerca

12 Articoli
Accesso libero

Molecular identification of Nematodirus spathiger (Nematoda: Molineidae) in Lama guanicoe from Patagonia, Argentina

Pubblicato online: 26 Jun 2014
Pagine: 79 - 82

Astratto

Abstract

The guanaco (Lama guanicoe) is the major inhabitant and the largest wild artiodactyl in Patagonia. The introduction of invasive species into its ecological niche poses ecological risks, since invasive species may introduce harmful parasites to this native species. In this work, filariform larvae of the Nematodirus genus were found in feces of guanacos from the Perito Moreno National Park in Argentina. All species were characterized according to morphological features and molecular analyses using ribosomal DNA (rDNA). For the molecular analysis, rDNA fragments were amplified by PCR and then sequenced. The results of the BLASTN comparison threw a 99 % of identity with Nematodirus spathiger and 97 % with N. helvetianus, suggesting that N. spathiger is the infecting parasite. Nematodirus spathiger together with N. filicollis and N. battus causes diarrhea and deaths in sheep and, in some cases, in South American camelids. The availability of more accurate diagnostic methods such as PCR could improve the control measures for gastrointestinal helminthiasis.

Parole chiave

  • filariform larvae
  • coproparasitology
  • PCR
  • ribosomal DNA (rDNA)
Accesso libero

Prevalence and genetic diversity of Oesophagostomum stephanostomum in wild lowland gorillas at Moukalaba-Doudou National Park, Gabon

Pubblicato online: 26 Jun 2014
Pagine: 83 - 93

Astratto

Abstract

Using a sedimentation method, the prevalence of the nodular worm Oesophagostomum stephanostomum (Nematoda: Strongylida) in western lowland gorillas at Moukalaba-Doudou National Park (MDNP), Gabon, was determined in fecal samples collected between January 2007 and October 2011, along with their coprocultures. Concurrently, possible zoonotic Oesophagostomum infections in villagers living near MDNP were assessed from their fecal samples collected during October and November of 2011. In the gorillas, strongylid (Oesophagostomum and/or hookworm) eggs were found in 47 of 235 fecal samples (20.0 %) and Oesophagostomum larvae were detected in 101 of 229 coprocultures (44.1 %). In the villagers, strongylid eggs were found in 9 of 71 fecal samples (12.7 %), but no Oesophagostomum larvae were detected in coprocultures. The internal transcribed spacer (ITS) region of ribosomal RNA gene (rDNA) and cytochrome c oxidase subunit-1 (cox-1) region of mitochondrial DNA (mtDNA) of coprocultured Oesophagostomum larvae were amplified using parasite DNA extracted from 7–25 larvae/sample, cloned into Escherichia coli, and sequenced. Sequenced rDNA contained 353/354-bp long ITS1, 151-bp long 5.8S rDNA, and 227-bp long ITS2. Parts of clones showed variations at 1–3 bases in the ITS1 region at a frequency of 24/68 (35.3 %) and at 1–2 bases in the ITS2 region at a frequency of 7/68 (10.3 %), whereas the 5.8S rDNA was essentially identical. Sequenced cox-1 gene of the parasites, 849 bp in length, showed a higher number of nucleotide variations, mainly at the third nucleotide position of the codon. The majority of clones (27/41 (65.9 %)) had an identical amino acid sequence. These results suggest that at MDNP, Gabon, only a single population of O. stephanostomum with a degree of genetic diversity is prevalent in western lowland gorillas, without zoonotic complication in local inhabitants. The possible genetic variations in the ITS region of rDNA and cox-1gene of mtDNA presented here may be valuable when only a limited amount of material is available for the molecular species diagnosis of O. stephanostomum.

Parole chiave

  • Oesophagostomum stephanostomum
  • western lowland gorilla
  • Gabon
  • genetic diversity
  • internal transcribed spacer (ITS)
  • cox-1
Accesso libero

Seroprevalence of Fasciola hepatica infection in cattle and sheep in the province of Kars, Turkey, as determined by ELISA

Pubblicato online: 26 Jun 2014
Pagine: 94 - 97

Astratto

Abstract

This study aimed to determine the seroprevalence of Fasciola hepatica infection in cattle and sheep in the province of Kars, Turkey. Serum samples from 500 cattle and 540 sheep, collected from 15 randomly selected localities (villages) in the region, were tested for the presence of anti-F. hepatica antibodies using an in-house ELISA test with 98 % sensitivity and 96 % specificity. The seroprevalence of F. hepatica in the district was determined to be 66.6 % (333/500) in cattle and 93 % (502/540) in sheep. There was also a statistically significant difference in the rates of seropositivity between villages (each of which could be considered to be a large herd or flock), ranging from 0 % to 100 % in cattle and from 68 % to 100 % in sheep, P < 0.01). These findings show that F. hepatica infection is very common in the region; that, in contrast to results from abattoir which indicate a level of only 10 % prevalence, the exposure of farm animals to the infection in the region is in fact very high; and that the risk of acquiring the infection varies between the localities and the host species tested within the region.

Parole chiave

  • Fasciola
  • ELISA
  • cattle
  • sheep
  • Kars
  • Turkey
Accesso libero

Endoparasitoses in hospitalised paediatric patients with pulmonary disease

Pubblicato online: 26 Jun 2014
Pagine: 98 - 102

Astratto

Abstract

Faeces examination of hospitalised paediatric patients with respiratory diseases (recurrent and chronic bronchitis, bronchial asthma, contact with TBC, active TBC, rhinopharyngitis, bronchitis, pneumonia, cystic fibrosis, fluidothorax, pleuropneumonia) revealed the total prevalence of endoparasites of 19.85 %, out of which the prevalence of helminth was 7.35 %. Following genera, or species were represented Ascaris lumbricoides, Trichuris trichuria, Hymenolepis spp., Enterobius vermicularis. The total prevalence of protozoa was 12.50 % in the following representation: Cryptosporidium spp., Entamoeba spp., Isospora spp., Giardia spp. Out of helminthoses the greatest representation was in Ascaris lumbricoides 4.41 %, and out of protozoa Cryptosporidium spp. 6.62 %. In paediatric patients the increased levels of IgE antibodies were found for individual age groups as well as higher values of eosinophiles (Eo > 5 %), lymphocytes (Lym > 56 %), and Creactive protein (CRP > 8 mg.l−1). Statistically significantly (P < 0.05) higher level (above the reference values) of IgE, Eo, Lym were found in the patients with helminthoses.

Parole chiave

  • endoparasitosis
  • helminths
  • protozoa
  • bronchial and pulmonary infections of children
  • prevalence
  • epidemiology
Accesso libero

The impact of grazing management on seasonal activity of gastrointestinal parasites in goats

Pubblicato online: 26 Jun 2014
Pagine: 103 - 111

Astratto

Abstract

The aim of this study was to examine the impact of grazing management and other risk factors (age, treatment practices) on seasonal activity of gastrointestinal (GI) parasites in goats. Goat flocks naturally infected with GI parasites reared on four Lithuanian farms representing different management regimes were examined during the grazing season in 2011/2012. On three farms the adult goats were grazed in different ways on open pastures (with or without supplementary feeding) or tethered. On one farm all animals were kept indoor (zero-grazing). On each farm, samples were collected at monthly intervals from 13–15 adult and 10 kids. The results showed that grazing of adult goats with feed supplementation or kept indoor, shed the lowest number of strongyle eggs when compared to those kept on pasture (P < 0.05). Delayed turnout and zero-grazing significantly reduced excretion of strongyle eggs but increased the output of oocysts when compared to those grazed on set-stocked pasture together with adult goats. The most prevalent genus on all farms and in both age groups of goats were Teladorsagia spp. This study demonstrates that goats are infected with mixed species of parasites, but proportions of these parasites differed in different grazing management systems. The grazing management, age and season were all major factors that had an impact on GI parasite infection.

Parole chiave

  • goats
  • grazing management
  • risk factors
  • nematodes
  • Eimeria spp
Accesso libero

On gastrointestinal nematodes of Mongolian gazelle (Procapra gutturosa)

Pubblicato online: 26 Jun 2014
Pagine: 112 - 116

Astratto

Abstract

The species composition of nematodes found at autopsy of abomasa and small intestines of 24 Mongolian gazelles in Eastern Mongolia is studied. Orloffia bisonis, Marshallagia mongolica, Nematodirus archari, N. andreevi, Trichostrongylus colubriformis, T. probolurus were registered. N. archari and N. andreevi were detected in Mongolian gazelle for the first time. All species of gastrointestinal nematodes found in Mongolian gazelles have already been registered in domestic ruminants of Mongolia. The validity of Orloffia genus is confirmed based on our own observations and literature data. Orloffia is monotypic genus with the only species O. bisonis represented by two morphs where “O. bisonis” is major and “O. kasakhstanica” is minor.

Parole chiave

  • Eastern Mongolia
  • gastrointestinal nematodes
  • Procapra gutturosa
Accesso libero

Helminth community structure study on urban and forest blackbird (Turdus merula L.) populations in relation to seasonal bird migration on the south Baltic Sea coast (NW Poland)

Pubblicato online: 26 Jun 2014
Pagine: 117 - 129

Astratto

Abstract

The aim of the study was to compare helminth community structure of urban and forest blackbird populations. 24 helminth species in 98 blackbirds were found. Higher species richness was noted in the forest population of the blackbird (23 species) in comparison to the urban population (14 species). The response of the helminth fauna to a synanthropic habitat, contrary to a natural habitat, consists in a significant reduction in most parasitological parameters. Higher species richness has been noted in spring (17 species) than in autumn (14 species). Urban habitat, in contrast to the forest, may cause changes in the abundance of helminth communities in male and female blackbirds. The helminth fauna of nestlings, in spite of low species richness is characterized by a higher prevalence and intensity of infection in comparison to blackbirds feeding on their own. Helminth fauna of the blackbird seems to be a good indicator of environmental quality.

Parole chiave

  • Turdus merula
  • helminths
  • urban
  • forest
  • migrations
Accesso libero

Comparative morphological and molecular identification of Haemonchus species in sheep

Pubblicato online: 26 Jun 2014
Pagine: 130 - 140

Astratto

Abstract

A combined approach in the determination of Haemonchus nematodes from sheep was applied in this trial. Using selected morphological characters 90.2 % females and 84.2 % males of Haemonchus contortus and 9.8 % females and 15.8 % males of Haemonchus placei were identified. Although cluster analysis based on morphological identification clearly separated two Haemonchus species, H. contortus was exclusively detected in all specimens using restriction cleavage of the ITS-2 region with FspBI endonuclease as well as through the sequencing analysis. Because sheep from both farms have never had contact with other ruminants, and the farmers apply only closed flock turnover, we assume that only H. contortus mono-infection occurred on both farms. This opinion is also supported by molecular data. The most striking result of our study was the finding which indicates that the discriminant function is not able to accurately identify Haemonchus males at the species level.

Parole chiave

  • Barber’s pole worm
  • spicule
  • discriminant function
  • PCR-RFLP
  • sequencing
  • internal transcribed spacer
Accesso libero

Morphological and molecular characterization of Posthodiplostomum sp. (Digenea: Diplostomidae) metacercaria in the muscles of snakeheads (Channa punctata) from Manipur, India

Pubblicato online: 26 Jun 2014
Pagine: 141 - 152

Astratto

Abstract

The spotted snakehead, Channa punctata Bloch, 1793, is a locally important fish species commonly consumed by the natives in the state of Manipur, Northeast India. The fish host C. punctata from Lamphel area revealed a diplostomid metacercarial infection. Morphologically, the recovered metacercaria was identified as a species of Posthodiplostomum Dubois, 1936. Molecular characterization using the ribosomal RNA genes (rDNA 18S, ITS2 and 28S regions) and the mitochondrial CO1 region supplements the identification. Molecular analysis revealed the metacercaria to be closely related to Posthodiplostomum sp. Japan isolate, with sequence similarity variation from 97.5–99.7 % while considering for the three rDNA markers. The secondary structure of the ITS2 region further corroborated these results; the typical four-helix model, when compared to the taxon from Japan, showed differences only in twelve bases (with seven transitions and five transversions). In phylogenetic analysis also, the metacercaria claded with the genus Posthodiplostomum, coming closer to the Japanese isolate, thus supplementing the morphological identification of the metacercaria.

Parole chiave

  • fish
  • Channa punctata; metacercaria
  • Posthodiplostomum sp.
  • morphology
  • morphometry
  • rDNA
  • mtCO1
Accesso libero

Characterization of Longidorus caespiticola (Nematoda: Longidoridae) from the Czech Republic

Pubblicato online: 26 Jun 2014
Pagine: 153 - 157

Astratto

Abstract

Longidorus caespiticola was found for the first time from the rhizosphere of apple with two other Longidorus sp. at Žlunice, Czech Republic. Females and males were analyzed morphologically and morphometrically. Four regions (18S, ITS1, ITS2, D2-D3 expansion segments of 28S) of ribosomal DNA and one region (cox1) of mitochondrial DNA were sequenced and analyzed and compared with a population from the Slovak Republic. The partial mitochondrial cytochrome c oxidase subunit 1 (cox1) gene showed relatively high genetic variation compared with ribosomal DNA between the Czech and Slovakian populations.

Parole chiave

  • Longidorus caespiticola
  • Mitochondrial DNA
  • Nematode
  • Ribosomal DNA
  • Sequencing
Accesso libero

Serological survey for sparganum infection in people of central China

Pubblicato online: 26 Jun 2014
Pagine: 158 - 161

Astratto

Abstract

During 2006–2008, twenty cases with sparganosis caused by eating live tadpoles emerged in Henan province, central China. To determine seroprevalence of anti-sparganum antibodies and obtain information about habits of eating live tadpoles and risks for sparganum infection, a serological survey was carried out in one village of Henan. Antisparganum IgG in 298 serum samples were assayed by ELISA using excretory and secretory (ES) antigens of Spirometra mansoni spargana. The results showed 56.71 % (169/298) of inhabitants had the history of eating live tadpoles. The overall seroprevalence was 5.7 % (17/298). The seroprevalence of the inhabitants who had the habit of eating tadpoles (9.47 %) was obviously higher than those who did not (0.78 %) (P < 0.01). Eating live tadpoles had become the most common risk behavior for sparganum infection. Hence, the comprehensive public health education should be carried out in endemic areas, and the habit of eating live tadpoles must be discouraged.

Parole chiave

  • Sparganosis
  • prevalence
  • ELISA
  • tadpole
  • Central China
Accesso libero

Steinernema kraussei (Steiner, 1923) (Rhabditida: Steinernematidae) — the first record from Poland

Pubblicato online: 26 Jun 2014
Pagine: 162 - 166

Astratto

Abstract

This study reports the first record of Steinernema kraussei from Poland. The nematode was isolated from coniferous woodlands in 4 localities in central Poland. Preliminary identification of the species was done based on morphometric measurements. To confirm nematode species of the genus Steinernema the result was supported by the description of the ITS region.

Parole chiave

  • entomopathogenic nematodes
  • Steinernemakraussei
  • localities in Poland
12 Articoli
Accesso libero

Molecular identification of Nematodirus spathiger (Nematoda: Molineidae) in Lama guanicoe from Patagonia, Argentina

Pubblicato online: 26 Jun 2014
Pagine: 79 - 82

Astratto

Abstract

The guanaco (Lama guanicoe) is the major inhabitant and the largest wild artiodactyl in Patagonia. The introduction of invasive species into its ecological niche poses ecological risks, since invasive species may introduce harmful parasites to this native species. In this work, filariform larvae of the Nematodirus genus were found in feces of guanacos from the Perito Moreno National Park in Argentina. All species were characterized according to morphological features and molecular analyses using ribosomal DNA (rDNA). For the molecular analysis, rDNA fragments were amplified by PCR and then sequenced. The results of the BLASTN comparison threw a 99 % of identity with Nematodirus spathiger and 97 % with N. helvetianus, suggesting that N. spathiger is the infecting parasite. Nematodirus spathiger together with N. filicollis and N. battus causes diarrhea and deaths in sheep and, in some cases, in South American camelids. The availability of more accurate diagnostic methods such as PCR could improve the control measures for gastrointestinal helminthiasis.

Parole chiave

  • filariform larvae
  • coproparasitology
  • PCR
  • ribosomal DNA (rDNA)
Accesso libero

Prevalence and genetic diversity of Oesophagostomum stephanostomum in wild lowland gorillas at Moukalaba-Doudou National Park, Gabon

Pubblicato online: 26 Jun 2014
Pagine: 83 - 93

Astratto

Abstract

Using a sedimentation method, the prevalence of the nodular worm Oesophagostomum stephanostomum (Nematoda: Strongylida) in western lowland gorillas at Moukalaba-Doudou National Park (MDNP), Gabon, was determined in fecal samples collected between January 2007 and October 2011, along with their coprocultures. Concurrently, possible zoonotic Oesophagostomum infections in villagers living near MDNP were assessed from their fecal samples collected during October and November of 2011. In the gorillas, strongylid (Oesophagostomum and/or hookworm) eggs were found in 47 of 235 fecal samples (20.0 %) and Oesophagostomum larvae were detected in 101 of 229 coprocultures (44.1 %). In the villagers, strongylid eggs were found in 9 of 71 fecal samples (12.7 %), but no Oesophagostomum larvae were detected in coprocultures. The internal transcribed spacer (ITS) region of ribosomal RNA gene (rDNA) and cytochrome c oxidase subunit-1 (cox-1) region of mitochondrial DNA (mtDNA) of coprocultured Oesophagostomum larvae were amplified using parasite DNA extracted from 7–25 larvae/sample, cloned into Escherichia coli, and sequenced. Sequenced rDNA contained 353/354-bp long ITS1, 151-bp long 5.8S rDNA, and 227-bp long ITS2. Parts of clones showed variations at 1–3 bases in the ITS1 region at a frequency of 24/68 (35.3 %) and at 1–2 bases in the ITS2 region at a frequency of 7/68 (10.3 %), whereas the 5.8S rDNA was essentially identical. Sequenced cox-1 gene of the parasites, 849 bp in length, showed a higher number of nucleotide variations, mainly at the third nucleotide position of the codon. The majority of clones (27/41 (65.9 %)) had an identical amino acid sequence. These results suggest that at MDNP, Gabon, only a single population of O. stephanostomum with a degree of genetic diversity is prevalent in western lowland gorillas, without zoonotic complication in local inhabitants. The possible genetic variations in the ITS region of rDNA and cox-1gene of mtDNA presented here may be valuable when only a limited amount of material is available for the molecular species diagnosis of O. stephanostomum.

Parole chiave

  • Oesophagostomum stephanostomum
  • western lowland gorilla
  • Gabon
  • genetic diversity
  • internal transcribed spacer (ITS)
  • cox-1
Accesso libero

Seroprevalence of Fasciola hepatica infection in cattle and sheep in the province of Kars, Turkey, as determined by ELISA

Pubblicato online: 26 Jun 2014
Pagine: 94 - 97

Astratto

Abstract

This study aimed to determine the seroprevalence of Fasciola hepatica infection in cattle and sheep in the province of Kars, Turkey. Serum samples from 500 cattle and 540 sheep, collected from 15 randomly selected localities (villages) in the region, were tested for the presence of anti-F. hepatica antibodies using an in-house ELISA test with 98 % sensitivity and 96 % specificity. The seroprevalence of F. hepatica in the district was determined to be 66.6 % (333/500) in cattle and 93 % (502/540) in sheep. There was also a statistically significant difference in the rates of seropositivity between villages (each of which could be considered to be a large herd or flock), ranging from 0 % to 100 % in cattle and from 68 % to 100 % in sheep, P < 0.01). These findings show that F. hepatica infection is very common in the region; that, in contrast to results from abattoir which indicate a level of only 10 % prevalence, the exposure of farm animals to the infection in the region is in fact very high; and that the risk of acquiring the infection varies between the localities and the host species tested within the region.

Parole chiave

  • Fasciola
  • ELISA
  • cattle
  • sheep
  • Kars
  • Turkey
Accesso libero

Endoparasitoses in hospitalised paediatric patients with pulmonary disease

Pubblicato online: 26 Jun 2014
Pagine: 98 - 102

Astratto

Abstract

Faeces examination of hospitalised paediatric patients with respiratory diseases (recurrent and chronic bronchitis, bronchial asthma, contact with TBC, active TBC, rhinopharyngitis, bronchitis, pneumonia, cystic fibrosis, fluidothorax, pleuropneumonia) revealed the total prevalence of endoparasites of 19.85 %, out of which the prevalence of helminth was 7.35 %. Following genera, or species were represented Ascaris lumbricoides, Trichuris trichuria, Hymenolepis spp., Enterobius vermicularis. The total prevalence of protozoa was 12.50 % in the following representation: Cryptosporidium spp., Entamoeba spp., Isospora spp., Giardia spp. Out of helminthoses the greatest representation was in Ascaris lumbricoides 4.41 %, and out of protozoa Cryptosporidium spp. 6.62 %. In paediatric patients the increased levels of IgE antibodies were found for individual age groups as well as higher values of eosinophiles (Eo > 5 %), lymphocytes (Lym > 56 %), and Creactive protein (CRP > 8 mg.l−1). Statistically significantly (P < 0.05) higher level (above the reference values) of IgE, Eo, Lym were found in the patients with helminthoses.

Parole chiave

  • endoparasitosis
  • helminths
  • protozoa
  • bronchial and pulmonary infections of children
  • prevalence
  • epidemiology
Accesso libero

The impact of grazing management on seasonal activity of gastrointestinal parasites in goats

Pubblicato online: 26 Jun 2014
Pagine: 103 - 111

Astratto

Abstract

The aim of this study was to examine the impact of grazing management and other risk factors (age, treatment practices) on seasonal activity of gastrointestinal (GI) parasites in goats. Goat flocks naturally infected with GI parasites reared on four Lithuanian farms representing different management regimes were examined during the grazing season in 2011/2012. On three farms the adult goats were grazed in different ways on open pastures (with or without supplementary feeding) or tethered. On one farm all animals were kept indoor (zero-grazing). On each farm, samples were collected at monthly intervals from 13–15 adult and 10 kids. The results showed that grazing of adult goats with feed supplementation or kept indoor, shed the lowest number of strongyle eggs when compared to those kept on pasture (P < 0.05). Delayed turnout and zero-grazing significantly reduced excretion of strongyle eggs but increased the output of oocysts when compared to those grazed on set-stocked pasture together with adult goats. The most prevalent genus on all farms and in both age groups of goats were Teladorsagia spp. This study demonstrates that goats are infected with mixed species of parasites, but proportions of these parasites differed in different grazing management systems. The grazing management, age and season were all major factors that had an impact on GI parasite infection.

Parole chiave

  • goats
  • grazing management
  • risk factors
  • nematodes
  • Eimeria spp
Accesso libero

On gastrointestinal nematodes of Mongolian gazelle (Procapra gutturosa)

Pubblicato online: 26 Jun 2014
Pagine: 112 - 116

Astratto

Abstract

The species composition of nematodes found at autopsy of abomasa and small intestines of 24 Mongolian gazelles in Eastern Mongolia is studied. Orloffia bisonis, Marshallagia mongolica, Nematodirus archari, N. andreevi, Trichostrongylus colubriformis, T. probolurus were registered. N. archari and N. andreevi were detected in Mongolian gazelle for the first time. All species of gastrointestinal nematodes found in Mongolian gazelles have already been registered in domestic ruminants of Mongolia. The validity of Orloffia genus is confirmed based on our own observations and literature data. Orloffia is monotypic genus with the only species O. bisonis represented by two morphs where “O. bisonis” is major and “O. kasakhstanica” is minor.

Parole chiave

  • Eastern Mongolia
  • gastrointestinal nematodes
  • Procapra gutturosa
Accesso libero

Helminth community structure study on urban and forest blackbird (Turdus merula L.) populations in relation to seasonal bird migration on the south Baltic Sea coast (NW Poland)

Pubblicato online: 26 Jun 2014
Pagine: 117 - 129

Astratto

Abstract

The aim of the study was to compare helminth community structure of urban and forest blackbird populations. 24 helminth species in 98 blackbirds were found. Higher species richness was noted in the forest population of the blackbird (23 species) in comparison to the urban population (14 species). The response of the helminth fauna to a synanthropic habitat, contrary to a natural habitat, consists in a significant reduction in most parasitological parameters. Higher species richness has been noted in spring (17 species) than in autumn (14 species). Urban habitat, in contrast to the forest, may cause changes in the abundance of helminth communities in male and female blackbirds. The helminth fauna of nestlings, in spite of low species richness is characterized by a higher prevalence and intensity of infection in comparison to blackbirds feeding on their own. Helminth fauna of the blackbird seems to be a good indicator of environmental quality.

Parole chiave

  • Turdus merula
  • helminths
  • urban
  • forest
  • migrations
Accesso libero

Comparative morphological and molecular identification of Haemonchus species in sheep

Pubblicato online: 26 Jun 2014
Pagine: 130 - 140

Astratto

Abstract

A combined approach in the determination of Haemonchus nematodes from sheep was applied in this trial. Using selected morphological characters 90.2 % females and 84.2 % males of Haemonchus contortus and 9.8 % females and 15.8 % males of Haemonchus placei were identified. Although cluster analysis based on morphological identification clearly separated two Haemonchus species, H. contortus was exclusively detected in all specimens using restriction cleavage of the ITS-2 region with FspBI endonuclease as well as through the sequencing analysis. Because sheep from both farms have never had contact with other ruminants, and the farmers apply only closed flock turnover, we assume that only H. contortus mono-infection occurred on both farms. This opinion is also supported by molecular data. The most striking result of our study was the finding which indicates that the discriminant function is not able to accurately identify Haemonchus males at the species level.

Parole chiave

  • Barber’s pole worm
  • spicule
  • discriminant function
  • PCR-RFLP
  • sequencing
  • internal transcribed spacer
Accesso libero

Morphological and molecular characterization of Posthodiplostomum sp. (Digenea: Diplostomidae) metacercaria in the muscles of snakeheads (Channa punctata) from Manipur, India

Pubblicato online: 26 Jun 2014
Pagine: 141 - 152

Astratto

Abstract

The spotted snakehead, Channa punctata Bloch, 1793, is a locally important fish species commonly consumed by the natives in the state of Manipur, Northeast India. The fish host C. punctata from Lamphel area revealed a diplostomid metacercarial infection. Morphologically, the recovered metacercaria was identified as a species of Posthodiplostomum Dubois, 1936. Molecular characterization using the ribosomal RNA genes (rDNA 18S, ITS2 and 28S regions) and the mitochondrial CO1 region supplements the identification. Molecular analysis revealed the metacercaria to be closely related to Posthodiplostomum sp. Japan isolate, with sequence similarity variation from 97.5–99.7 % while considering for the three rDNA markers. The secondary structure of the ITS2 region further corroborated these results; the typical four-helix model, when compared to the taxon from Japan, showed differences only in twelve bases (with seven transitions and five transversions). In phylogenetic analysis also, the metacercaria claded with the genus Posthodiplostomum, coming closer to the Japanese isolate, thus supplementing the morphological identification of the metacercaria.

Parole chiave

  • fish
  • Channa punctata; metacercaria
  • Posthodiplostomum sp.
  • morphology
  • morphometry
  • rDNA
  • mtCO1
Accesso libero

Characterization of Longidorus caespiticola (Nematoda: Longidoridae) from the Czech Republic

Pubblicato online: 26 Jun 2014
Pagine: 153 - 157

Astratto

Abstract

Longidorus caespiticola was found for the first time from the rhizosphere of apple with two other Longidorus sp. at Žlunice, Czech Republic. Females and males were analyzed morphologically and morphometrically. Four regions (18S, ITS1, ITS2, D2-D3 expansion segments of 28S) of ribosomal DNA and one region (cox1) of mitochondrial DNA were sequenced and analyzed and compared with a population from the Slovak Republic. The partial mitochondrial cytochrome c oxidase subunit 1 (cox1) gene showed relatively high genetic variation compared with ribosomal DNA between the Czech and Slovakian populations.

Parole chiave

  • Longidorus caespiticola
  • Mitochondrial DNA
  • Nematode
  • Ribosomal DNA
  • Sequencing
Accesso libero

Serological survey for sparganum infection in people of central China

Pubblicato online: 26 Jun 2014
Pagine: 158 - 161

Astratto

Abstract

During 2006–2008, twenty cases with sparganosis caused by eating live tadpoles emerged in Henan province, central China. To determine seroprevalence of anti-sparganum antibodies and obtain information about habits of eating live tadpoles and risks for sparganum infection, a serological survey was carried out in one village of Henan. Antisparganum IgG in 298 serum samples were assayed by ELISA using excretory and secretory (ES) antigens of Spirometra mansoni spargana. The results showed 56.71 % (169/298) of inhabitants had the history of eating live tadpoles. The overall seroprevalence was 5.7 % (17/298). The seroprevalence of the inhabitants who had the habit of eating tadpoles (9.47 %) was obviously higher than those who did not (0.78 %) (P < 0.01). Eating live tadpoles had become the most common risk behavior for sparganum infection. Hence, the comprehensive public health education should be carried out in endemic areas, and the habit of eating live tadpoles must be discouraged.

Parole chiave

  • Sparganosis
  • prevalence
  • ELISA
  • tadpole
  • Central China
Accesso libero

Steinernema kraussei (Steiner, 1923) (Rhabditida: Steinernematidae) — the first record from Poland

Pubblicato online: 26 Jun 2014
Pagine: 162 - 166

Astratto

Abstract

This study reports the first record of Steinernema kraussei from Poland. The nematode was isolated from coniferous woodlands in 4 localities in central Poland. Preliminary identification of the species was done based on morphometric measurements. To confirm nematode species of the genus Steinernema the result was supported by the description of the ITS region.

Parole chiave

  • entomopathogenic nematodes
  • Steinernemakraussei
  • localities in Poland

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