Rivista e Edizione

Volume 59 (2022): Edizione 2 (June 2022)

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Volume 58 (2021): Edizione 2 (June 2021)

Volume 58 (2021): Edizione 1 (March 2021)

Volume 57 (2020): Edizione 4 (December 2020)

Volume 57 (2020): Edizione 3 (September 2020)

Volume 57 (2020): Edizione 2 (June 2020)

Volume 57 (2020): Edizione 1 (March 2020)

Volume 56 (2019): Edizione 4 (December 2019)

Volume 56 (2019): Edizione 3 (September 2019)

Volume 56 (2019): Edizione 2 (June 2019)

Volume 56 (2019): Edizione 1 (March 2019)

Volume 55 (2018): Edizione 4 (December 2018)

Volume 55 (2018): Edizione 3 (September 2018)

Volume 55 (2018): Edizione 2 (June 2018)

Volume 55 (2018): Edizione 1 (March 2018)

Volume 54 (2017): Edizione 4 (December 2017)
Special Edizione: This issue is dedicated to the memory of Milan Ryboš, DVM, D.Sc.

Volume 54 (2017): Edizione 3 (September 2017)

Volume 54 (2017): Edizione 2 (June 2017)

Volume 54 (2017): Edizione 1 (March 2017)

Volume 53 (2016): Edizione 4 (December 2016)

Volume 53 (2016): Edizione 3 (September 2016)

Volume 53 (2016): Edizione 2 (June 2016)

Volume 53 (2016): Edizione 1 (March 2016)

Volume 52 (2015): Edizione 4 (December 2015)

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Volume 52 (2015): Edizione 2 (June 2015)

Volume 52 (2015): Edizione 1 (March 2015)

Volume 51 (2014): Edizione 4 (December 2014)

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Volume 51 (2014): Edizione 2 (June 2014)

Volume 51 (2014): Edizione 1 (March 2014)

Volume 50 (2013): Edizione 4 (December 2013)

Volume 50 (2013): Edizione 3 (September 2013)

Volume 50 (2013): Edizione 2 (June 2013)

Volume 50 (2013): Edizione 1 (May 2013)

Volume 49 (2012): Edizione 4 (December 2012)

Volume 49 (2012): Edizione 3 (September 2012)

Volume 49 (2012): Edizione 2 (June 2012)

Volume 49 (2012): Edizione 1 (March 2012)

Volume 48 (2011): Edizione 4 (December 2011)

Volume 48 (2011): Edizione 3 (September 2011)

Volume 48 (2011): Edizione 2 (June 2011)

Volume 48 (2011): Edizione 1 (March 2011)

Volume 47 (2010): Edizione 4 (December 2010)

Volume 47 (2010): Edizione 3 (September 2010)

Volume 47 (2010): Edizione 2 (June 2010)

Volume 47 (2010): Edizione 1 (March 2010)

Volume 46 (2009): Edizione 4 (December 2009)

Volume 46 (2009): Edizione 3 (September 2009)

Volume 46 (2009): Edizione 2 (June 2009)

Volume 46 (2009): Edizione 1 (March 2009)

Volume 45 (2008): Edizione 4 (December 2008)

Volume 45 (2008): Edizione 3 (September 2008)

Volume 45 (2008): Edizione 2 (June 2008)

Volume 45 (2008): Edizione 1 (March 2008)

Volume 44 (2007): Edizione 4 (December 2007)

Volume 44 (2007): Edizione 3 (September 2007)

Volume 44 (2007): Edizione 2 (June 2007)

Volume 44 (2007): Edizione 1 (March 2007)

Volume 43 (2006): Edizione 4 (December 2006)

Volume 43 (2006): Edizione 3 (June 2006)

Volume 43 (2006): Edizione 2 (June 2006)

Volume 43 (2006): Edizione 1 (March 2006)

Dettagli della rivista
Formato
Rivista
eISSN
1336-9083
ISSN
0440-6605
Pubblicato per la prima volta
22 Apr 2006
Periodo di pubblicazione
4 volte all'anno
Lingue
Inglese

Cerca

Volume 45 (2008): Edizione 3 (September 2008)

Dettagli della rivista
Formato
Rivista
eISSN
1336-9083
ISSN
0440-6605
Pubblicato per la prima volta
22 Apr 2006
Periodo di pubblicazione
4 volte all'anno
Lingue
Inglese

Cerca

11 Articoli
access type Accesso libero

Note on morphology of two nematode species Ascaridia hermaphrodita and Ascaridia platyceri (Nematoda): scanning electron microscope study

Pubblicato online: 06 Nov 2008
Pagine: 109 - 113

Astratto

Abstract

Morphological features of adult specimens of Ascaridia hermaphrodita and A. platyceri (Nematoda: Ascaridida), parasitizing parrots (Psittaciformes), were studied for the first time using scanning electron microscopy. The morphology of A. hermaphrodita, the type species of Ascaridia, coincides fully with the generic diagnosis. The shape of the labial inner structure (two spoon-like projections), cervical alae and spicule with cuticular wings were typical for this species. A. hermaphrodita has been found in the Czech Republic for the first time in a new host, Amazona pretrei, from Brazil. The morphology of Ascaridia platyceri does not correspond with the generic diagnosis in all aspects, as some variation was found, mainly in the presence of interlabia, small teeth in the inner ribbon of the lips and long lateral alae. These morphological characteristics in other species of Ascaridia are discussed. The generic diagnosis is to include the statement “interlabia very rarely present”.

Parole chiave

  • Ascaridia hermaphrodita
  • A. platyceri
  • Psittaciformes
  • Czech Republic
  • morphology
  • differences
access type Accesso libero

Inhibition of Filarial Glutathione-S-transferase by various classes of compounds and their evaluation as novel antifilarial agents

Pubblicato online: 06 Nov 2008
Pagine: 114 - 120

Astratto

Abstract

Glutathione S-transferase(s); GST(s) (E.C. 2.5.1.18) are a large family of multifunctional dimeric enzymes that conjugate reduced glutathione to electrophilic centres in hydrophobic organic compounds. GST(s) represent the major class of detoxifying enzymes from parasitic helminths. The GST enzymatic activity has been described in the adult and larval stages of helminths. Several forms and isoforms of the enzyme have been purified and GST genes have also been isolated and expressed as recombinant proteins. The helminth GST(s) participate in detoxification of lipid hydroperoxides and cytotoxic carbonyl compounds produced by oxygen-reactive intermediates (ORIs). The ORIs can come from the endogenous parasite metabolism or from the host immune system. The helminth GST(s) are able to conjugate glutathione to xenobiotic compounds or to bind to the anthelminth drugs. GST is usually found to be localized near to host-parasite interface. This enzyme has been identified as a potentially vulnerable target in immunotherapy and chemotherapy of parasitic diseases. The most effective drug candidates are those based on inhibitors of GST. In the present study, purified GST from cytosolic fraction of bovine filarial worms Setaria cervi was inhibited in a concentration dependent fashion by various compounds such as hemin, ethacrynic acid, S-hexylglutathione, quercetin, cibacron blue, lithocholate sulfate and ellagic acid. Cytosolic GST was inhibited to varying degrees by each inhibitor. In this context, the possible physiological significance of the observed results has been discussed.

Parole chiave

  • Glutathione-S-transferase
  • enzyme inhibition
  • glutathione analog
  • Setaria cervi
  • helminth parasites
  • anti-parasitic agents
access type Accesso libero

Comparison of the histological methods in the diagnostic of deer cysticercosis

Pubblicato online: 06 Nov 2008
Pagine: 121 - 125

Astratto

Abstract

Histochemical methods for the detection and diagnosis of the developmental stages of the canine tapeworm, from the genus Taenia found in the heart and lungs of red deer (Cervus elaphus) and roe deer (Capreolus capreolus) hunted in Eastern Slovakia, is presented here. Detailed morphology of cysticerci (Cysticercus spp.), based on microscopic and histochemical analysis is described. For confirmation and demonstration of PAS-positive substances in the body of parasitic tissue (tegument and mesenchyme) the McManus — PAS method was used. The histochemical method according to Van Kossa was very effective for confirmation of calcareous corpuscles, which are one of the most important histological markers of cestode tissues (larva or adult).

Parole chiave

  • cysticercosis
  • deer
  • histochemistry
  • histology
access type Accesso libero

Helminth communities and host-parasite relationships in argentine brown rat (Rattus norvegicus)

Pubblicato online: 06 Nov 2008
Pagine: 126 - 129

Astratto

Abstract

The aims of this research were: 1) to determine the helminth parasite fauna of seventy two Rattus norvegicus (Berkenhout, 1769) individuals inhabiting poultry farms in the Pampean region of Argentina; 2) to examine the relationship between parasitism and individual characteristics of the hosts, and 3) to analyze the associations among helminths. The study was carried out in twenty-four poultry farms from autumn 2000 to winter 2001. Nematodes were found in the intestine or caecum rectum of the 74 % of rats and cestodes were found along all the intestine of the 28 % of rats. Heterakis spumosa (Travassos 1914) was a central species, whereas Syphacia muris, Yamaguti 1941, Nippostrongylus brasiliensis (Travassos 1914) and Hymenolepis diminuta (Rudolphi 1819) were satellite species. Helminths do not affect rat survival and growth of rats. Rats parasitized with H. spumosa and H. diminuta were larger and older than non-parasitized rats. The intensity of infection with H. spumosa significantly increased with rat age. Rats parasitized with Nippostrongylus brasiliensis and Syphacia muris did not differ in size and age with respect to non-parasitized ones. The mean intensity of infections per host was 33.74, 74.28, higher than 200, and 3.10 for H. spumosa, S. muris, N. brasiliensis and H. diminuta, respectively. The mean intensity of infection with H. spumosa was higher in summer than in the other seasons, while the higher mean intensity of infection with N. brasiliensis and S. muris occurred in autumn. Infections with Heterakis spumosa and Syphacia muris, and with Syphacia muris and Nippostrongylus brasiliensis were independent; while Heterakis spumosa and Nippostrongylus brasiliensis were negatively associated.

Parole chiave

  • helminths
  • Heterakis spumosa
  • Hymenolepis diminuta
  • Nippostrongylus brasiliensis
  • Rattus norvegicus
  • Syphacia muris
access type Accesso libero

Fuhrmanolepis dubinskyi n. sp. (Cestoda: Dilepididae) from woodcock Scolopax rusticola (L.) (Aves, Charadriiformes) in Slovakia

Pubblicato online: 06 Nov 2008
Pagine: 130 - 133

Astratto

Abstract

Dilepidid cestode Fuhrmanolepis dubinskyi n. sp. from woodcock, Scolopax rusticola L. in Slovakia is described on the basis of light microscope observations. The new taxon is characterised by medium sized strobila, single crown of ten, 47–53 μm long “diorchid” (wrench-shaped) rostellar hooks. Genital pores alternate irregularly. Number of testes is 20–30 and measurements of cirrus-sac and evaginated cirrus reach 176 − 217 × 18 − 31 and 38 − 56 × 16 − 21 μm, respectively. The uterus is reticular and eggs possess spiny embryophore. F. dubinskyi is differentiated from closely related congeneric species and some other morphologically similar dilepidids.

Parole chiave

  • Cestoda
  • Dilepididae
  • Fuhrmanolepis dubinskyi n. sp
  • Scolopax rusticola
  • Charadriiformes
  • Slovakia
access type Accesso libero

New records of mononchid nematodes from forests in the Slovak Republic

Pubblicato online: 06 Nov 2008
Pagine: 134 - 136

Astratto

Abstract

Three mononchid nematode species new for the Slovak Republic were recorded in forest ecosystems. In regularly-flooded forests with Fraxineto-Salicetum vegetation, the species Mylonchulus andrassyi and Tigronchoides ginglymodontus were recorded; in soil with the Querceto-Carpinetum forest type, the species Miconchus hopperi was observed. Comments on the morphometrical characteristics and ecology of the recorded species are presented here.

Parole chiave

  • Mononchida
  • Miconchus hopperi
  • Mylonchulus andrassyi
  • Tigronchoides ginglymodontus
  • forest
  • Slovakia
access type Accesso libero

Aphanocladium album by via sub-irrigation in the control of Pyrenochaeta lycopersici and Meloidogyne incognita on tomato in a plastic-house

Pubblicato online: 06 Nov 2008
Pagine: 137 - 142

Astratto

Abstract

Two experiments were carried out to assess the efficacy of different chemicals (azoxystrobin, fosthiazate, metham-sodium) and of the chitinolytic fungus Aphanocladium album (isolate MX-95), that could be alternatives to methyl bromide, against the soil borne pathogen Pyrenochaeta lycopersici and the root-knot nematode Meloidogyne incognita on tomato in a plastic house in southern Italy. In the first trial, the treatments were azoxystrobin (1.25 l a.i. /ha), fosthiazate (1.5 l a.i. /ha) and biological control agent Aphanocladium album isolate MX-95 (2.5 l/plot at 2×107 CFU/ml; plot surface 96 m2). In the second experiment, treatments were metham-sodium (1000 l c.p./ha) and A. album (5 l/plot at 1×107 CFU/ml). In both trials, chemicals and the fungus were applied by via sub-irrigation. Satisfactory control of the corky root and the root-knot nematode attack and a significant yield increase were obtained by application of azoxystrobin, fosthiazate and metham-sodium. A significant reduction of M. incognita soil population density occurred in plots treated with A. album. Also, high positive correlations were found between the symptoms caused on tomato roots by M. incognita and P. lycopersici.

Parole chiave

  • Biological control
  • corky root
  • root-knot nematodes
  • chemical treatments
  • sub-irrigation
access type Accesso libero

Occurrence and geographical distribution of cyst nematodes in cereals and grassland in the Slovak Republic

Pubblicato online: 06 Nov 2008
Pagine: 143 - 146

Astratto

Abstract

A study of occurrence and geographical distribution of cyst-forming nematodes in monocultural cereal fields and multispecies grassland vegetation in Slovak Republic revealed the presence of two species of Heterodera: H. avenae and H. schachtii. The frequency of occurrence of H. avenae was 37.8 % in cereal fields and 3.3 % in grassland. Cysts of H. schachtii were found only in cereal fields along with cysts of H. avenae, in the western part of country. The occurrence of other graminaceous cyst nematode species was not recorded.

Parole chiave

  • Heterodera
  • cyst nematodes
  • grassland
  • cereals
  • occurrence
  • Slovak Republic
access type Accesso libero

Detection of Toxocara canis larvae by PCR in the liver of experimentally infected Mongolian gerbils (Meriones unguiculatus)

Pubblicato online: 06 Nov 2008
Pagine: 147 - 149

Astratto

Abstract

Toxocariasis is a common human zoonosis, which induces a clinically unapparent course of infection. Diagnosis is difficult and relies upon serological testing (searching of specific IgG antibodies by ELISA), laboratory abnormalities and clinical manifestations. The polymerase chain reaction (PCR) technique was adapted for the detection of Toxocara canis larvae in a host tissue. Mongolian gerbils (Meriones unguiculatus) were used as an animal model for human toxocariasis. 8 animals were inoculated with 1000 T. canis eggs, four uninfected were used as control. At 3, 5, 7, and 14 days post-infection, 2 infected and 1 control gerbil were killed and their livers were used for molecular analysis. Specific primer in the PCR reaction allowed identification of T. canis larvae, with the parasite gDNA found in the liver of all infected gerbils. The results indicate that the PCR method has a potential as a supporting technique for the diagnosis of human toxocariasis.

Parole chiave

  • Toxocara canis
  • polymerase chain reaction (PCR)
  • Mongolian gerbils
  • toxocariasis
access type Accesso libero

Detection of Echinococcus granulosus coproantigens in dogs from Antakya Province, Turkey

Pubblicato online: 06 Nov 2008
Pagine: 150 - 153

Astratto

Abstract

Seventy-nine owned dogs (47 males and 32 females) from Antakya province were examined for E. granulosus by coproantigen ELISA (CHEKIT®-ECHINOTEST) between April to July in 2006. The estimated prevalence of E. granulosus infection was 8.86 % (7/79). Mean age of the dogs with E. granulosus was 2.3 years ranging from one to five years. There was no statistically significant difference regarding the prevalence between male and female dogs. A significant difference was detected between unrestrained (or free roaming) and indoor or chained dogs (p < 0.05). Although the prevalence of the E. granulosus infection in owned dogs in Antakya province is low, the incidence was quite high in the unrestrained and undewormed dogs. Therefore a preventive control programme in the dogs should be initiated in this region.

Parole chiave

  • Echinococcus granulosus
  • dog
  • coproantigen ELISA
  • Antakya
  • Turkey
access type Accesso libero

Occurence of Pomphorhynchus laevis (Acanthocephala) in the Marsh Frog (Rana ridibunda Pallas, 1771), from Turkey

Pubblicato online: 06 Nov 2008
Pagine: 154 - 156

Astratto

Abstract

Pomphorhynchus laevis (Müller, 1776) was recovered from Marsh frogs (Rana ridibunda), collected in three localities, Yesilirmak River (Amasya Province), Civril Isikli Lake and Bagbasi district (Denizli Province) from Turkey. This is the first report of Pomphorhynchus laevis from a frog in Turkey.

Parole chiave

  • Pomphorhynchus laevis
  • Rana ridibunda
  • Turkey
11 Articoli
access type Accesso libero

Note on morphology of two nematode species Ascaridia hermaphrodita and Ascaridia platyceri (Nematoda): scanning electron microscope study

Pubblicato online: 06 Nov 2008
Pagine: 109 - 113

Astratto

Abstract

Morphological features of adult specimens of Ascaridia hermaphrodita and A. platyceri (Nematoda: Ascaridida), parasitizing parrots (Psittaciformes), were studied for the first time using scanning electron microscopy. The morphology of A. hermaphrodita, the type species of Ascaridia, coincides fully with the generic diagnosis. The shape of the labial inner structure (two spoon-like projections), cervical alae and spicule with cuticular wings were typical for this species. A. hermaphrodita has been found in the Czech Republic for the first time in a new host, Amazona pretrei, from Brazil. The morphology of Ascaridia platyceri does not correspond with the generic diagnosis in all aspects, as some variation was found, mainly in the presence of interlabia, small teeth in the inner ribbon of the lips and long lateral alae. These morphological characteristics in other species of Ascaridia are discussed. The generic diagnosis is to include the statement “interlabia very rarely present”.

Parole chiave

  • Ascaridia hermaphrodita
  • A. platyceri
  • Psittaciformes
  • Czech Republic
  • morphology
  • differences
access type Accesso libero

Inhibition of Filarial Glutathione-S-transferase by various classes of compounds and their evaluation as novel antifilarial agents

Pubblicato online: 06 Nov 2008
Pagine: 114 - 120

Astratto

Abstract

Glutathione S-transferase(s); GST(s) (E.C. 2.5.1.18) are a large family of multifunctional dimeric enzymes that conjugate reduced glutathione to electrophilic centres in hydrophobic organic compounds. GST(s) represent the major class of detoxifying enzymes from parasitic helminths. The GST enzymatic activity has been described in the adult and larval stages of helminths. Several forms and isoforms of the enzyme have been purified and GST genes have also been isolated and expressed as recombinant proteins. The helminth GST(s) participate in detoxification of lipid hydroperoxides and cytotoxic carbonyl compounds produced by oxygen-reactive intermediates (ORIs). The ORIs can come from the endogenous parasite metabolism or from the host immune system. The helminth GST(s) are able to conjugate glutathione to xenobiotic compounds or to bind to the anthelminth drugs. GST is usually found to be localized near to host-parasite interface. This enzyme has been identified as a potentially vulnerable target in immunotherapy and chemotherapy of parasitic diseases. The most effective drug candidates are those based on inhibitors of GST. In the present study, purified GST from cytosolic fraction of bovine filarial worms Setaria cervi was inhibited in a concentration dependent fashion by various compounds such as hemin, ethacrynic acid, S-hexylglutathione, quercetin, cibacron blue, lithocholate sulfate and ellagic acid. Cytosolic GST was inhibited to varying degrees by each inhibitor. In this context, the possible physiological significance of the observed results has been discussed.

Parole chiave

  • Glutathione-S-transferase
  • enzyme inhibition
  • glutathione analog
  • Setaria cervi
  • helminth parasites
  • anti-parasitic agents
access type Accesso libero

Comparison of the histological methods in the diagnostic of deer cysticercosis

Pubblicato online: 06 Nov 2008
Pagine: 121 - 125

Astratto

Abstract

Histochemical methods for the detection and diagnosis of the developmental stages of the canine tapeworm, from the genus Taenia found in the heart and lungs of red deer (Cervus elaphus) and roe deer (Capreolus capreolus) hunted in Eastern Slovakia, is presented here. Detailed morphology of cysticerci (Cysticercus spp.), based on microscopic and histochemical analysis is described. For confirmation and demonstration of PAS-positive substances in the body of parasitic tissue (tegument and mesenchyme) the McManus — PAS method was used. The histochemical method according to Van Kossa was very effective for confirmation of calcareous corpuscles, which are one of the most important histological markers of cestode tissues (larva or adult).

Parole chiave

  • cysticercosis
  • deer
  • histochemistry
  • histology
access type Accesso libero

Helminth communities and host-parasite relationships in argentine brown rat (Rattus norvegicus)

Pubblicato online: 06 Nov 2008
Pagine: 126 - 129

Astratto

Abstract

The aims of this research were: 1) to determine the helminth parasite fauna of seventy two Rattus norvegicus (Berkenhout, 1769) individuals inhabiting poultry farms in the Pampean region of Argentina; 2) to examine the relationship between parasitism and individual characteristics of the hosts, and 3) to analyze the associations among helminths. The study was carried out in twenty-four poultry farms from autumn 2000 to winter 2001. Nematodes were found in the intestine or caecum rectum of the 74 % of rats and cestodes were found along all the intestine of the 28 % of rats. Heterakis spumosa (Travassos 1914) was a central species, whereas Syphacia muris, Yamaguti 1941, Nippostrongylus brasiliensis (Travassos 1914) and Hymenolepis diminuta (Rudolphi 1819) were satellite species. Helminths do not affect rat survival and growth of rats. Rats parasitized with H. spumosa and H. diminuta were larger and older than non-parasitized rats. The intensity of infection with H. spumosa significantly increased with rat age. Rats parasitized with Nippostrongylus brasiliensis and Syphacia muris did not differ in size and age with respect to non-parasitized ones. The mean intensity of infections per host was 33.74, 74.28, higher than 200, and 3.10 for H. spumosa, S. muris, N. brasiliensis and H. diminuta, respectively. The mean intensity of infection with H. spumosa was higher in summer than in the other seasons, while the higher mean intensity of infection with N. brasiliensis and S. muris occurred in autumn. Infections with Heterakis spumosa and Syphacia muris, and with Syphacia muris and Nippostrongylus brasiliensis were independent; while Heterakis spumosa and Nippostrongylus brasiliensis were negatively associated.

Parole chiave

  • helminths
  • Heterakis spumosa
  • Hymenolepis diminuta
  • Nippostrongylus brasiliensis
  • Rattus norvegicus
  • Syphacia muris
access type Accesso libero

Fuhrmanolepis dubinskyi n. sp. (Cestoda: Dilepididae) from woodcock Scolopax rusticola (L.) (Aves, Charadriiformes) in Slovakia

Pubblicato online: 06 Nov 2008
Pagine: 130 - 133

Astratto

Abstract

Dilepidid cestode Fuhrmanolepis dubinskyi n. sp. from woodcock, Scolopax rusticola L. in Slovakia is described on the basis of light microscope observations. The new taxon is characterised by medium sized strobila, single crown of ten, 47–53 μm long “diorchid” (wrench-shaped) rostellar hooks. Genital pores alternate irregularly. Number of testes is 20–30 and measurements of cirrus-sac and evaginated cirrus reach 176 − 217 × 18 − 31 and 38 − 56 × 16 − 21 μm, respectively. The uterus is reticular and eggs possess spiny embryophore. F. dubinskyi is differentiated from closely related congeneric species and some other morphologically similar dilepidids.

Parole chiave

  • Cestoda
  • Dilepididae
  • Fuhrmanolepis dubinskyi n. sp
  • Scolopax rusticola
  • Charadriiformes
  • Slovakia
access type Accesso libero

New records of mononchid nematodes from forests in the Slovak Republic

Pubblicato online: 06 Nov 2008
Pagine: 134 - 136

Astratto

Abstract

Three mononchid nematode species new for the Slovak Republic were recorded in forest ecosystems. In regularly-flooded forests with Fraxineto-Salicetum vegetation, the species Mylonchulus andrassyi and Tigronchoides ginglymodontus were recorded; in soil with the Querceto-Carpinetum forest type, the species Miconchus hopperi was observed. Comments on the morphometrical characteristics and ecology of the recorded species are presented here.

Parole chiave

  • Mononchida
  • Miconchus hopperi
  • Mylonchulus andrassyi
  • Tigronchoides ginglymodontus
  • forest
  • Slovakia
access type Accesso libero

Aphanocladium album by via sub-irrigation in the control of Pyrenochaeta lycopersici and Meloidogyne incognita on tomato in a plastic-house

Pubblicato online: 06 Nov 2008
Pagine: 137 - 142

Astratto

Abstract

Two experiments were carried out to assess the efficacy of different chemicals (azoxystrobin, fosthiazate, metham-sodium) and of the chitinolytic fungus Aphanocladium album (isolate MX-95), that could be alternatives to methyl bromide, against the soil borne pathogen Pyrenochaeta lycopersici and the root-knot nematode Meloidogyne incognita on tomato in a plastic house in southern Italy. In the first trial, the treatments were azoxystrobin (1.25 l a.i. /ha), fosthiazate (1.5 l a.i. /ha) and biological control agent Aphanocladium album isolate MX-95 (2.5 l/plot at 2×107 CFU/ml; plot surface 96 m2). In the second experiment, treatments were metham-sodium (1000 l c.p./ha) and A. album (5 l/plot at 1×107 CFU/ml). In both trials, chemicals and the fungus were applied by via sub-irrigation. Satisfactory control of the corky root and the root-knot nematode attack and a significant yield increase were obtained by application of azoxystrobin, fosthiazate and metham-sodium. A significant reduction of M. incognita soil population density occurred in plots treated with A. album. Also, high positive correlations were found between the symptoms caused on tomato roots by M. incognita and P. lycopersici.

Parole chiave

  • Biological control
  • corky root
  • root-knot nematodes
  • chemical treatments
  • sub-irrigation
access type Accesso libero

Occurrence and geographical distribution of cyst nematodes in cereals and grassland in the Slovak Republic

Pubblicato online: 06 Nov 2008
Pagine: 143 - 146

Astratto

Abstract

A study of occurrence and geographical distribution of cyst-forming nematodes in monocultural cereal fields and multispecies grassland vegetation in Slovak Republic revealed the presence of two species of Heterodera: H. avenae and H. schachtii. The frequency of occurrence of H. avenae was 37.8 % in cereal fields and 3.3 % in grassland. Cysts of H. schachtii were found only in cereal fields along with cysts of H. avenae, in the western part of country. The occurrence of other graminaceous cyst nematode species was not recorded.

Parole chiave

  • Heterodera
  • cyst nematodes
  • grassland
  • cereals
  • occurrence
  • Slovak Republic
access type Accesso libero

Detection of Toxocara canis larvae by PCR in the liver of experimentally infected Mongolian gerbils (Meriones unguiculatus)

Pubblicato online: 06 Nov 2008
Pagine: 147 - 149

Astratto

Abstract

Toxocariasis is a common human zoonosis, which induces a clinically unapparent course of infection. Diagnosis is difficult and relies upon serological testing (searching of specific IgG antibodies by ELISA), laboratory abnormalities and clinical manifestations. The polymerase chain reaction (PCR) technique was adapted for the detection of Toxocara canis larvae in a host tissue. Mongolian gerbils (Meriones unguiculatus) were used as an animal model for human toxocariasis. 8 animals were inoculated with 1000 T. canis eggs, four uninfected were used as control. At 3, 5, 7, and 14 days post-infection, 2 infected and 1 control gerbil were killed and their livers were used for molecular analysis. Specific primer in the PCR reaction allowed identification of T. canis larvae, with the parasite gDNA found in the liver of all infected gerbils. The results indicate that the PCR method has a potential as a supporting technique for the diagnosis of human toxocariasis.

Parole chiave

  • Toxocara canis
  • polymerase chain reaction (PCR)
  • Mongolian gerbils
  • toxocariasis
access type Accesso libero

Detection of Echinococcus granulosus coproantigens in dogs from Antakya Province, Turkey

Pubblicato online: 06 Nov 2008
Pagine: 150 - 153

Astratto

Abstract

Seventy-nine owned dogs (47 males and 32 females) from Antakya province were examined for E. granulosus by coproantigen ELISA (CHEKIT®-ECHINOTEST) between April to July in 2006. The estimated prevalence of E. granulosus infection was 8.86 % (7/79). Mean age of the dogs with E. granulosus was 2.3 years ranging from one to five years. There was no statistically significant difference regarding the prevalence between male and female dogs. A significant difference was detected between unrestrained (or free roaming) and indoor or chained dogs (p < 0.05). Although the prevalence of the E. granulosus infection in owned dogs in Antakya province is low, the incidence was quite high in the unrestrained and undewormed dogs. Therefore a preventive control programme in the dogs should be initiated in this region.

Parole chiave

  • Echinococcus granulosus
  • dog
  • coproantigen ELISA
  • Antakya
  • Turkey
access type Accesso libero

Occurence of Pomphorhynchus laevis (Acanthocephala) in the Marsh Frog (Rana ridibunda Pallas, 1771), from Turkey

Pubblicato online: 06 Nov 2008
Pagine: 154 - 156

Astratto

Abstract

Pomphorhynchus laevis (Müller, 1776) was recovered from Marsh frogs (Rana ridibunda), collected in three localities, Yesilirmak River (Amasya Province), Civril Isikli Lake and Bagbasi district (Denizli Province) from Turkey. This is the first report of Pomphorhynchus laevis from a frog in Turkey.

Parole chiave

  • Pomphorhynchus laevis
  • Rana ridibunda
  • Turkey

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