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Special Edizione: This issue is dedicated to the memory of Milan Ryboš, DVM, D.Sc.

Volume 54 (2017): Edizione 3 (September 2017)

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Volume 47 (2010): Edizione 4 (December 2010)

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Volume 47 (2010): Edizione 2 (June 2010)

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Volume 46 (2009): Edizione 4 (December 2009)

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Volume 45 (2008): Edizione 4 (December 2008)

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Volume 45 (2008): Edizione 2 (June 2008)

Volume 45 (2008): Edizione 1 (March 2008)

Volume 44 (2007): Edizione 4 (December 2007)

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Volume 44 (2007): Edizione 2 (June 2007)

Volume 44 (2007): Edizione 1 (March 2007)

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Volume 43 (2006): Edizione 2 (June 2006)

Volume 43 (2006): Edizione 1 (March 2006)

Dettagli della rivista
Formato
Rivista
eISSN
1336-9083
ISSN
0440-6605
Pubblicato per la prima volta
22 Apr 2006
Periodo di pubblicazione
4 volte all'anno
Lingue
Inglese

Cerca

Volume 45 (2008): Edizione 1 (March 2008)

Dettagli della rivista
Formato
Rivista
eISSN
1336-9083
ISSN
0440-6605
Pubblicato per la prima volta
22 Apr 2006
Periodo di pubblicazione
4 volte all'anno
Lingue
Inglese

Cerca

10 Articoli
Accesso libero

Molecular genotyping of Anisakis Dujardin, 1845 (Nematoda: Ascaridoidea: Anisakidae) larvae from marine fish of Balinese and Javanese waters, Indonesia

Pubblicato online: 02 Mar 2008
Pagine: 3 - 12

Astratto

Abstract

The genetic identification and distribution of Anisakis larvae in Indonesia is described. 110 Auxis rochei rochei and 45 Decapterus russellii were sampled from fish markets in North (Anturan) and South (Kedonganan) Bali. Nematode larvae from A. rochei rochei, Caesio cuning and Epinephelus areolatus from Kedonganan and from Coryphaena hippurus from Pelabuhan Ratu, South Java, were identified using sequence analysis of the internal transcribed spacers (ITS-1, ITS-2) and 5.8S region of rDNA. The larvae belonged to Anisakis typica with an identical sequence to this species from the spinner dolphin (Stenella longirostris) from Brazil, and to 2 further genotypes that differed from that sequence by 0.24–0.47 %. A. typica occurred in the migratory A. rochei rochei and C. hippurus, while Anisakis sp. 1 and 2 were isolated from the same fish species and the non-migratory C. cuning and E. areolatus. The latter genotype is distinguishable by 4 positions in the ITS-1 region (1.1 %), a genetic distance that indicates the presence of an Indonesian A. typica sibling species. The musculature infection in A. rochei rochei was low (2.5 %), indicating no major risk for the fish consumers. The much higher A. typica infection of fish intermediate hosts in the northern Bali coast is suggested to be dependent on the large dolphin population (nematode final hosts) in the waters off Lovina Beach (North Bali).

Parole chiave

  • Anisakis typica
  • Bali
  • Java
  • Indonesia
  • molecular genotyping
  • sibling species
  • zoogeography
Accesso libero

Wild carnivores as source of zoonotic helminths in north-eastern Italy

Pubblicato online: 02 Mar 2008
Pagine: 13 - 19

Astratto

Abstract

Two hundreds and sixty red foxes, eighteen badgers and eight stone martens from north-eastern Italy were examined for zoonotic parasites by SCT, with particular attention to Trichinella sp. and Echinococcus multilocularis. No adult worms of E. multilocularis were observed in the intestine of red foxes or mustelids. Out of 223 fox faecal samples analysed by a commercial CA-ELISA, 5.8 % was positive to Echinococcus coproantigens. Trichinella sp. was detected by digestion of muscle in 1.2 % of examined foxes (2/172), but not in mustelids (0/11). Toxocara canis, responsible for human toxocarosis, resulted to be common in fox population (48.5 %). This parasite was found both in young (53.3 % of cubs and 61.9 % of sub-adults) and in adult animals (42.7 %). Other zoonotic parasites as Uncinaria stenocephala (52.3 %) and Trichuris vulpis (0.4 %) were detected in fox populations.

Parole chiave

  • red fox
  • badger
  • stone marten
  • helminths
  • zoonoses
  • Italy
Accesso libero

Dirofilariosis in Slovakia - a new endemic area in Central Europe

Pubblicato online: 02 Mar 2008
Pagine: 20 - 23

Astratto

Abstract

The first systematic research on canine dirofilariosis in Slovakia started in February 2007. In total 287 dogs of various age, sex, breeds, and utilization from southern Slovakia were examined within the study until September 2007. Microfilariaemia was detected in 99 (34.5 %) blood samples. Histochemical staining and PCR approach were used for Dirofilaria species identification. Dirofilaria repens was confirmed in all infected dogs; it occurred in 92 (32.1 %) dogs as a single infection and in 6 (2.1 %) dogs in mixed infection with Dirofilaria immitis. For the first time both species were detected in Eastern Slovakia. Outdoor keeping and age higher 3 years become evident as important risk factors. Male individuals and dogs with long hair were significantly more frequently infected when compared with females and short hair breeds.

The present study revealed the occurrence of highly endemic area of dirofilariosis in Slovakia and certified that climatic conditions in the country are favourable for development of the parasite and for further spread of this zoonotic infection.

Parole chiave

  • microfilaria
  • Dirofilaria repens
  • Dirofilaria immitis
  • dogs
  • Slovakia
Accesso libero

Occurence of Echinococcus multilocularis in red foxes (Vulpes vulpes) in southern Poland

Pubblicato online: 02 Mar 2008
Pagine: 24 - 27

Astratto

Abstract

The prevalence of Echinococcus multilocularis in 214 red foxes (Vulpes vulpes) in the southern part of Poland (Małopolskie voivodship) was evaluated post mortem in 2005. Infected foxes were found in 8 districts within 17 examined, with the prevalence from 10.0 % to 63.9 % (a mean 20.1 % in the whole study area). The highest prevalence (41.2 % and 63.9 %) was revealed in two districts, in the south and the east of the province, respectively. The high prevalence of E. multilocularis in red foxes in the region previously recognised as that of low parasite endemicity provides evidence for the need of regular screening of the current epidemiological situation in foxes as well as the monitoring of humans from risk groups for early recognition of possible AE cases.

Parole chiave

  • Echinococcus multilocularis
  • red fox
  • prevalence
  • Poland
Accesso libero

A rare case of alveolar echinococcosis in a 14-year-old child

Pubblicato online: 02 Mar 2008
Pagine: 28 - 31

Astratto

Abstract

We report the case of a 14-year-old girl who suffered from headache, fatigue, caught and a fever with body temperature up to 40 °C for a week. The patient had abdominal enlargement and clinical examination showed the hepatomegaly +5 cm. In ELISA assays employing Em2+ antigen, high titres of specific IgG antibodies to Echinococcus multilocularis somatic antigens were found in the serum. Western blot analysis also indicated on presence of alveolar echinococcosis (AE). CT examination showed expansive foci in the right liver lobe and histological examinations of the biopsy after PAS staining confirmed the presence of alveolar echinococcosis. After 320 days of chemotherapy with albendazole, CT revealed progression of parasitic foci and new abscess lesions inside parasitic cysts were detected, with some parts being necrotic. The surgical removing of foci was performed as following — the laparotomic revision, the removal of the right liver lobe with dilatation, reconstruction of v. cavae cum prothesis PTFE in length 12 cm. Three months after the operation, the rest of the patient’s left liver lobe had grownup and compensated at least a part of the removed liver.

Parole chiave

  • alveolar echinococcosis
  • surgery
  • treatment
  • CT imaging
  • antibody response
Accesso libero

The influence of desiccation and UV radiation on the development and survival of free-living stages of cyathostomins under field and laboratory conditions

Pubblicato online: 02 Mar 2008
Pagine: 32 - 40

Astratto

Abstract

The present work describing both laboratory and field experiments was performed to assess the effects of desiccation and UV radiation on the development and survival of free-living stages of equine cyathostomins.

Cyathostomin larvae in horse faeces did not develop to the infective stage when faecal humidity levels dropped below 23 %, nonetheless solitary preinfective larvae were still recovered after 151 days (humidity 19.5 %). The development to infective stage after remoistening occurred for the last time after 54 days following desiccation.

Preinfective stages are susceptible to the effects of the direct desiccation stage. The preinfective larvae were rapidly killed within one minute, the cyathostomin eggs within 5 hours. The numerous normal mobile infective larvae were encountered after 35 days of the desiccated period. The preinfective stage of cyathostomins also showed very little tolerance to direct sun radiation: most eggs were killed by the exposure within 3 hours and the preinfective larvae within 1 hour. The survival of infective larvae was, on the other hand, unaffected by sun radiation after 7 days (P < 0.05). However, desiccated infective larvae were then found to be susceptible to UV radiation, resulting in total mortalities after 5 days.

Parole chiave

  • cyathostomins
  • equine strongyles
  • free-living stages
  • desiccation
  • UV radiation
Accesso libero

Occurrence of acanthocephalans in the Eurasian otter Lutra lutra (L.) (Carnivora, Mustelidae) in Bulgaria, with a survey of acanthocephalans recorded from this host species

Pubblicato online: 02 Mar 2008
Pagine: 41 - 47

Astratto

Abstract

Three Eurasian otters (Lutra lutra) originating from Pazardzhik Region (South Bulgaria) were examined for helminth parasites. Three species of acanthocephalans were recorded: Pomphorhynchus laevis, Acanthocephalus anguillae and A. ranae. These species are not specific parasites of otters, the former two occurring in various freshwater fishes and the latter being a parasite of anurans. A review of acanthocephalan species recorded from L. lutra is presented. The possible transmission routes by which otters become infested with acanthocephalans are considered to be either paratenic transfer of encysted extra-intestinal juveniles in paratenic hosts or postcyclic transmission of mature intestinal worms in definitive hosts.

Parole chiave

  • Acanthocephala
  • Eurasian otter
  • Bulgaria
Accesso libero

Helminth parasites of the pupfish Cyprinodon meeki (Pisces: Cyprinodontiformes), an endemic freshwater fish from North-Central Mexico

Pubblicato online: 02 Mar 2008
Pagine: 48 - 51

Astratto

Abstract

A total of 21 pupfish Cyprindon meeki was collected from the spring Abraham González, Durango, Mexico. Seven helminth taxa were recovered: Salsuginus angularis, Posthodiplostomum minimum, Clinostomum complanatum, Bothriocephalus acheilognathi, Cyclustera cf. ralli, Atactorhynchus duranguensis and Spiroxys sp. Among them, B. acheilognathi and A. duranguensis were the most frequent and abundant species. The remaining species were rare and found at low mean abundance. The observed species richness, individual parasite abundance, and diversity were low at component community and infracommunity levels. These features are in accordance with those recorded for other freshwater fish species in the Nearctic part of Mexico. Host feeding habits, along with its role as intermediate host, are suggested as the main factors in determining the helminth community structure in this fish species.

Parole chiave

  • Pupfish
  • Cyprinodon meeki
  • helminth community
  • Mexico
Accesso libero

Occurrence of the root-knot nematode Meloidogyne arenaria on balm and in a mixed population with M. javanica on grapevine in Greece

Pubblicato online: 02 Mar 2008
Pagine: 52 - 53

Astratto

Abstract

The root-knot nematode Meloidogyne arenaria was found in Greece infecting balm (Melissa officinalis L.) and grapevine (Vitis vinifera L.). The isolate from balm was M. arenaria while the one from grapevine was a mixture of M. arenaria and M. javanica (prevalent species). This is the first report of the M. arenaria species in the country in which identification was based on biochemical methods and its occurrence on balm is a new host record.

Parole chiave

  • esterase phenotypes
  • Melissa officinalis
  • Vitis vinifera
Accesso libero

Attachment of Pasteuria penetrans spores on populations of Meloidogyne javanica and M. incognita virulent and avirulent on the Mi gene of resistant tomato

Pubblicato online: 02 Mar 2008
Pagine: 54 - 56

Astratto

Abstract

Spore attachment of three Pasteuria penetrans isolates was assessed on juveniles of Meloidogyne incognita and Meloidogyne javanica populations from Greece. The nematode populations differed in their ability to reproduce on tomatoes with the Mi gene, conferring resistance to rootknot nematodes. The numbers of attached spores did not discriminate virulent from avirulent populations within each species. The differences in attachment rates probably reflect the specificity of Pasteuria penetrans to different Meloidogyne populations of the same species, within a country or even the same area.

Parole chiave

  • biocontrol
  • root-knot nematodes
10 Articoli
Accesso libero

Molecular genotyping of Anisakis Dujardin, 1845 (Nematoda: Ascaridoidea: Anisakidae) larvae from marine fish of Balinese and Javanese waters, Indonesia

Pubblicato online: 02 Mar 2008
Pagine: 3 - 12

Astratto

Abstract

The genetic identification and distribution of Anisakis larvae in Indonesia is described. 110 Auxis rochei rochei and 45 Decapterus russellii were sampled from fish markets in North (Anturan) and South (Kedonganan) Bali. Nematode larvae from A. rochei rochei, Caesio cuning and Epinephelus areolatus from Kedonganan and from Coryphaena hippurus from Pelabuhan Ratu, South Java, were identified using sequence analysis of the internal transcribed spacers (ITS-1, ITS-2) and 5.8S region of rDNA. The larvae belonged to Anisakis typica with an identical sequence to this species from the spinner dolphin (Stenella longirostris) from Brazil, and to 2 further genotypes that differed from that sequence by 0.24–0.47 %. A. typica occurred in the migratory A. rochei rochei and C. hippurus, while Anisakis sp. 1 and 2 were isolated from the same fish species and the non-migratory C. cuning and E. areolatus. The latter genotype is distinguishable by 4 positions in the ITS-1 region (1.1 %), a genetic distance that indicates the presence of an Indonesian A. typica sibling species. The musculature infection in A. rochei rochei was low (2.5 %), indicating no major risk for the fish consumers. The much higher A. typica infection of fish intermediate hosts in the northern Bali coast is suggested to be dependent on the large dolphin population (nematode final hosts) in the waters off Lovina Beach (North Bali).

Parole chiave

  • Anisakis typica
  • Bali
  • Java
  • Indonesia
  • molecular genotyping
  • sibling species
  • zoogeography
Accesso libero

Wild carnivores as source of zoonotic helminths in north-eastern Italy

Pubblicato online: 02 Mar 2008
Pagine: 13 - 19

Astratto

Abstract

Two hundreds and sixty red foxes, eighteen badgers and eight stone martens from north-eastern Italy were examined for zoonotic parasites by SCT, with particular attention to Trichinella sp. and Echinococcus multilocularis. No adult worms of E. multilocularis were observed in the intestine of red foxes or mustelids. Out of 223 fox faecal samples analysed by a commercial CA-ELISA, 5.8 % was positive to Echinococcus coproantigens. Trichinella sp. was detected by digestion of muscle in 1.2 % of examined foxes (2/172), but not in mustelids (0/11). Toxocara canis, responsible for human toxocarosis, resulted to be common in fox population (48.5 %). This parasite was found both in young (53.3 % of cubs and 61.9 % of sub-adults) and in adult animals (42.7 %). Other zoonotic parasites as Uncinaria stenocephala (52.3 %) and Trichuris vulpis (0.4 %) were detected in fox populations.

Parole chiave

  • red fox
  • badger
  • stone marten
  • helminths
  • zoonoses
  • Italy
Accesso libero

Dirofilariosis in Slovakia - a new endemic area in Central Europe

Pubblicato online: 02 Mar 2008
Pagine: 20 - 23

Astratto

Abstract

The first systematic research on canine dirofilariosis in Slovakia started in February 2007. In total 287 dogs of various age, sex, breeds, and utilization from southern Slovakia were examined within the study until September 2007. Microfilariaemia was detected in 99 (34.5 %) blood samples. Histochemical staining and PCR approach were used for Dirofilaria species identification. Dirofilaria repens was confirmed in all infected dogs; it occurred in 92 (32.1 %) dogs as a single infection and in 6 (2.1 %) dogs in mixed infection with Dirofilaria immitis. For the first time both species were detected in Eastern Slovakia. Outdoor keeping and age higher 3 years become evident as important risk factors. Male individuals and dogs with long hair were significantly more frequently infected when compared with females and short hair breeds.

The present study revealed the occurrence of highly endemic area of dirofilariosis in Slovakia and certified that climatic conditions in the country are favourable for development of the parasite and for further spread of this zoonotic infection.

Parole chiave

  • microfilaria
  • Dirofilaria repens
  • Dirofilaria immitis
  • dogs
  • Slovakia
Accesso libero

Occurence of Echinococcus multilocularis in red foxes (Vulpes vulpes) in southern Poland

Pubblicato online: 02 Mar 2008
Pagine: 24 - 27

Astratto

Abstract

The prevalence of Echinococcus multilocularis in 214 red foxes (Vulpes vulpes) in the southern part of Poland (Małopolskie voivodship) was evaluated post mortem in 2005. Infected foxes were found in 8 districts within 17 examined, with the prevalence from 10.0 % to 63.9 % (a mean 20.1 % in the whole study area). The highest prevalence (41.2 % and 63.9 %) was revealed in two districts, in the south and the east of the province, respectively. The high prevalence of E. multilocularis in red foxes in the region previously recognised as that of low parasite endemicity provides evidence for the need of regular screening of the current epidemiological situation in foxes as well as the monitoring of humans from risk groups for early recognition of possible AE cases.

Parole chiave

  • Echinococcus multilocularis
  • red fox
  • prevalence
  • Poland
Accesso libero

A rare case of alveolar echinococcosis in a 14-year-old child

Pubblicato online: 02 Mar 2008
Pagine: 28 - 31

Astratto

Abstract

We report the case of a 14-year-old girl who suffered from headache, fatigue, caught and a fever with body temperature up to 40 °C for a week. The patient had abdominal enlargement and clinical examination showed the hepatomegaly +5 cm. In ELISA assays employing Em2+ antigen, high titres of specific IgG antibodies to Echinococcus multilocularis somatic antigens were found in the serum. Western blot analysis also indicated on presence of alveolar echinococcosis (AE). CT examination showed expansive foci in the right liver lobe and histological examinations of the biopsy after PAS staining confirmed the presence of alveolar echinococcosis. After 320 days of chemotherapy with albendazole, CT revealed progression of parasitic foci and new abscess lesions inside parasitic cysts were detected, with some parts being necrotic. The surgical removing of foci was performed as following — the laparotomic revision, the removal of the right liver lobe with dilatation, reconstruction of v. cavae cum prothesis PTFE in length 12 cm. Three months after the operation, the rest of the patient’s left liver lobe had grownup and compensated at least a part of the removed liver.

Parole chiave

  • alveolar echinococcosis
  • surgery
  • treatment
  • CT imaging
  • antibody response
Accesso libero

The influence of desiccation and UV radiation on the development and survival of free-living stages of cyathostomins under field and laboratory conditions

Pubblicato online: 02 Mar 2008
Pagine: 32 - 40

Astratto

Abstract

The present work describing both laboratory and field experiments was performed to assess the effects of desiccation and UV radiation on the development and survival of free-living stages of equine cyathostomins.

Cyathostomin larvae in horse faeces did not develop to the infective stage when faecal humidity levels dropped below 23 %, nonetheless solitary preinfective larvae were still recovered after 151 days (humidity 19.5 %). The development to infective stage after remoistening occurred for the last time after 54 days following desiccation.

Preinfective stages are susceptible to the effects of the direct desiccation stage. The preinfective larvae were rapidly killed within one minute, the cyathostomin eggs within 5 hours. The numerous normal mobile infective larvae were encountered after 35 days of the desiccated period. The preinfective stage of cyathostomins also showed very little tolerance to direct sun radiation: most eggs were killed by the exposure within 3 hours and the preinfective larvae within 1 hour. The survival of infective larvae was, on the other hand, unaffected by sun radiation after 7 days (P < 0.05). However, desiccated infective larvae were then found to be susceptible to UV radiation, resulting in total mortalities after 5 days.

Parole chiave

  • cyathostomins
  • equine strongyles
  • free-living stages
  • desiccation
  • UV radiation
Accesso libero

Occurrence of acanthocephalans in the Eurasian otter Lutra lutra (L.) (Carnivora, Mustelidae) in Bulgaria, with a survey of acanthocephalans recorded from this host species

Pubblicato online: 02 Mar 2008
Pagine: 41 - 47

Astratto

Abstract

Three Eurasian otters (Lutra lutra) originating from Pazardzhik Region (South Bulgaria) were examined for helminth parasites. Three species of acanthocephalans were recorded: Pomphorhynchus laevis, Acanthocephalus anguillae and A. ranae. These species are not specific parasites of otters, the former two occurring in various freshwater fishes and the latter being a parasite of anurans. A review of acanthocephalan species recorded from L. lutra is presented. The possible transmission routes by which otters become infested with acanthocephalans are considered to be either paratenic transfer of encysted extra-intestinal juveniles in paratenic hosts or postcyclic transmission of mature intestinal worms in definitive hosts.

Parole chiave

  • Acanthocephala
  • Eurasian otter
  • Bulgaria
Accesso libero

Helminth parasites of the pupfish Cyprinodon meeki (Pisces: Cyprinodontiformes), an endemic freshwater fish from North-Central Mexico

Pubblicato online: 02 Mar 2008
Pagine: 48 - 51

Astratto

Abstract

A total of 21 pupfish Cyprindon meeki was collected from the spring Abraham González, Durango, Mexico. Seven helminth taxa were recovered: Salsuginus angularis, Posthodiplostomum minimum, Clinostomum complanatum, Bothriocephalus acheilognathi, Cyclustera cf. ralli, Atactorhynchus duranguensis and Spiroxys sp. Among them, B. acheilognathi and A. duranguensis were the most frequent and abundant species. The remaining species were rare and found at low mean abundance. The observed species richness, individual parasite abundance, and diversity were low at component community and infracommunity levels. These features are in accordance with those recorded for other freshwater fish species in the Nearctic part of Mexico. Host feeding habits, along with its role as intermediate host, are suggested as the main factors in determining the helminth community structure in this fish species.

Parole chiave

  • Pupfish
  • Cyprinodon meeki
  • helminth community
  • Mexico
Accesso libero

Occurrence of the root-knot nematode Meloidogyne arenaria on balm and in a mixed population with M. javanica on grapevine in Greece

Pubblicato online: 02 Mar 2008
Pagine: 52 - 53

Astratto

Abstract

The root-knot nematode Meloidogyne arenaria was found in Greece infecting balm (Melissa officinalis L.) and grapevine (Vitis vinifera L.). The isolate from balm was M. arenaria while the one from grapevine was a mixture of M. arenaria and M. javanica (prevalent species). This is the first report of the M. arenaria species in the country in which identification was based on biochemical methods and its occurrence on balm is a new host record.

Parole chiave

  • esterase phenotypes
  • Melissa officinalis
  • Vitis vinifera
Accesso libero

Attachment of Pasteuria penetrans spores on populations of Meloidogyne javanica and M. incognita virulent and avirulent on the Mi gene of resistant tomato

Pubblicato online: 02 Mar 2008
Pagine: 54 - 56

Astratto

Abstract

Spore attachment of three Pasteuria penetrans isolates was assessed on juveniles of Meloidogyne incognita and Meloidogyne javanica populations from Greece. The nematode populations differed in their ability to reproduce on tomatoes with the Mi gene, conferring resistance to rootknot nematodes. The numbers of attached spores did not discriminate virulent from avirulent populations within each species. The differences in attachment rates probably reflect the specificity of Pasteuria penetrans to different Meloidogyne populations of the same species, within a country or even the same area.

Parole chiave

  • biocontrol
  • root-knot nematodes

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