Rivista e Edizione

Volume 59 (2022): Edizione 2 (June 2022)

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Volume 58 (2021): Edizione 2 (June 2021)

Volume 58 (2021): Edizione 1 (March 2021)

Volume 57 (2020): Edizione 4 (December 2020)

Volume 57 (2020): Edizione 3 (September 2020)

Volume 57 (2020): Edizione 2 (June 2020)

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Volume 56 (2019): Edizione 4 (December 2019)

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Volume 56 (2019): Edizione 2 (June 2019)

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Volume 55 (2018): Edizione 4 (December 2018)

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Volume 55 (2018): Edizione 2 (June 2018)

Volume 55 (2018): Edizione 1 (March 2018)

Volume 54 (2017): Edizione 4 (December 2017)
Special Edizione: This issue is dedicated to the memory of Milan Ryboš, DVM, D.Sc.

Volume 54 (2017): Edizione 3 (September 2017)

Volume 54 (2017): Edizione 2 (June 2017)

Volume 54 (2017): Edizione 1 (March 2017)

Volume 53 (2016): Edizione 4 (December 2016)

Volume 53 (2016): Edizione 3 (September 2016)

Volume 53 (2016): Edizione 2 (June 2016)

Volume 53 (2016): Edizione 1 (March 2016)

Volume 52 (2015): Edizione 4 (December 2015)

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Volume 51 (2014): Edizione 4 (December 2014)

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Volume 50 (2013): Edizione 4 (December 2013)

Volume 50 (2013): Edizione 3 (September 2013)

Volume 50 (2013): Edizione 2 (June 2013)

Volume 50 (2013): Edizione 1 (May 2013)

Volume 49 (2012): Edizione 4 (December 2012)

Volume 49 (2012): Edizione 3 (September 2012)

Volume 49 (2012): Edizione 2 (June 2012)

Volume 49 (2012): Edizione 1 (March 2012)

Volume 48 (2011): Edizione 4 (December 2011)

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Volume 48 (2011): Edizione 2 (June 2011)

Volume 48 (2011): Edizione 1 (March 2011)

Volume 47 (2010): Edizione 4 (December 2010)

Volume 47 (2010): Edizione 3 (September 2010)

Volume 47 (2010): Edizione 2 (June 2010)

Volume 47 (2010): Edizione 1 (March 2010)

Volume 46 (2009): Edizione 4 (December 2009)

Volume 46 (2009): Edizione 3 (September 2009)

Volume 46 (2009): Edizione 2 (June 2009)

Volume 46 (2009): Edizione 1 (March 2009)

Volume 45 (2008): Edizione 4 (December 2008)

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Volume 45 (2008): Edizione 2 (June 2008)

Volume 45 (2008): Edizione 1 (March 2008)

Volume 44 (2007): Edizione 4 (December 2007)

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Volume 44 (2007): Edizione 2 (June 2007)

Volume 44 (2007): Edizione 1 (March 2007)

Volume 43 (2006): Edizione 4 (December 2006)

Volume 43 (2006): Edizione 3 (June 2006)

Volume 43 (2006): Edizione 2 (June 2006)

Volume 43 (2006): Edizione 1 (March 2006)

Dettagli della rivista
Formato
Rivista
eISSN
1336-9083
ISSN
0440-6605
Pubblicato per la prima volta
22 Apr 2006
Periodo di pubblicazione
4 volte all'anno
Lingue
Inglese

Cerca

Volume 50 (2013): Edizione 2 (June 2013)

Dettagli della rivista
Formato
Rivista
eISSN
1336-9083
ISSN
0440-6605
Pubblicato per la prima volta
22 Apr 2006
Periodo di pubblicazione
4 volte all'anno
Lingue
Inglese

Cerca

12 Articoli
access type Accesso libero

Effect of low temperatures on the infectivity of Toxocara cati larvae parasitized in mouse tissues

Pubblicato online: 31 Aug 2013
Pagine: 83 - 86

Astratto

Abstract

The infectivity of Toxocara cati larvae in mouse tissues, following storage at 4 °C and −25 °C, was measured using a bioassay in mice. Tissues of donor mice infected with 30-day-old T. cati larvae were stored at 4 °C for 7 and 14 days, and at −25 °C for 24 hours, then tissue larvae were inoculated into recipient mice (n = 6/group). After 15 days, larval burden in recipient mice was assessed by digestion. In the control group, a mean of 42.0 % of the inoculated larvae were established in mice. Storage of tissues at 4 °C for 7 and 14 days did not affect larval infectivity; means of 43.5 % and 37.4 % of inoculated larvae, respectively, were established in mice. Larvae exposed to −25 °C for 24 hours did not establish in mice, except for one larva recovered from a single mouse. These results may support the potential of tissue larvae in livestock animals as a causative agent of human toxocarosis.

Parole chiave

  • Toxocara cati
  • tissue larvae
  • cold tolerance
  • zoonosis
access type Accesso libero

Human hydatidosis: an under discussed occupational zoonosis in India

Pubblicato online: 31 Aug 2013
Pagine: 87 - 90

Astratto

Abstract

Human hydatidosis is an important public health issue in India and many other developing countries. There is lack of data related to occurrence of this disease in certain risk groups. The present study was therefore designed to assess the seroprevalence of human hydatidosis among occupational risk groups in Punjab (India). One hundred and forty nine human serum samples from dog handlers, veterinarians/para veterinarians, dairy farmers, patients with liver disorders, HIV positives and control group (visiting hospital for other problems) were collected and analyzed using a commercially available enzyme linked immunosorbent assay (ELISA). The results revealed that 23 (15.43 %) subjects were seropositive for human hydatidosis. High seropositivity were recorded in dog handlers (30 %), followed by the other risk group populations. Relative risk of being infected was found to be 4.80 times high in dog handlers when compared to control group population. Future studies must be planned to determine the significance of these findings and the status of human hydatidosis in India.

Parole chiave

  • seroprevalence
  • human hydatidosis
  • occupational risk groups
  • India
access type Accesso libero

Efficacy of monepantel against lower developmental stages of a multi-resistant and susceptible Haemonchus contortus isolates: an in vitro study

Pubblicato online: 31 Aug 2013
Pagine: 91 - 95

Astratto

Abstract

Monepantel (MOP) belongs to a new class of anthelmintic compounds, the amino-acetonitrile derivates, which have a different mode of action as the currently used anthelmintics. Many present studies confirmed the high efficacy of MOP against fourth larval and adult stages of Haemonchus contortus. The objective of this study was to determine in vitro efficacy of MOP against lower development stages (eggs, L1–L3 larvae) and to compare it between resistant and susceptible isolates of H. contortus. For this purpose, two in vitro tests - egg hatch test and micro-agar larval development test were used. Results were quantified as 50 % lethal concentration (LC50), 99 % lethal concentration (LC99) and resistance factor (RF). This study revealed the high efficacy against lower larval stages (L1–L3) of both resistant and susceptible strains of this parasite. Larval susceptibility was not dependent of the sensitivity status of the nematode isolate. On the other hand, ovicidal effect of MOP was very low.

Parole chiave

  • amino-acetonitrile derivatives
  • antihelmintic resistance
  • micro-agar larval development test
  • monepantel
  • nematode
access type Accesso libero

Cystic echinococcosis in Lombardy: epidemiological aspects and spatial analysis

Pubblicato online: 31 Aug 2013
Pagine: 96 - 103

Astratto

Abstract

A descriptive spatial analysis on the spreading of cattle cystic echinococcosis in non-endemic areas of Italy (Lombardy) was performed and the presence of clusters of hydatid disease in the studied area were evaluated. Positive cattle were from 285 out of 5708 farms (4.99 %) with significant differences among the provinces considered. Infection with Echinococcus granulosus was detected in 321 out of 112,521 dairy cattle (P = 0.29 %). CE-positive farms were distributed among 195 of the 812 municipalities included in the study (24.01 %) located in the central and south-eastern part of the region. By SaTScan, four significant clusters of infection were identified; they included 4–241 cattle farms and 4–24 positive animals. The most likely spatial cluster of 17 observed cases in 241 farms was located in the north-eastern part of Lombardy. SaTScan analysis suggest that a geographical surveillance in the area of the positive clusters occur is for and that’s could be very useful to public health service to monitoring this zoonotic disease.

Parole chiave

  • cattle
  • Echinococcus granulosus
  • Italy
  • spatial analysis
  • cluster
access type Accesso libero

Mesothelial metaplasia in European pond turtle, Emys orbicularis (Testudines: Emydidae) infected with Spiroxys contortus (Nematoda: Spirurida)

Pubblicato online: 31 Aug 2013
Pagine: 104 - 107

Astratto

Abstract

The mesothelium consists of a monolayer of specialized epithelial cells that line the surfaces of the peritoneal, pleural, and pericardial cavities. The present study reports mesothelial changes associated with larval stages of the spirurid nematode Spiroxys contortus in a naturally infected European pond turtle. Between 2002 and 2007, eight European pond turtle, Emys orbicularis were brought for necropsy. In one adult male turtle, necropsy revealed the presence of gastric and mesothelial changes associated with gastric infestation with the nematode Spiroxys contortus. Gastrointestinal samples with gross lesions were collected and processed by paraffin technique for further histological examination. Mesothelial cuboidal to columnar or pseudostratified focal progressive metaplasia was observed in visceral coelomic cavity. Intracytoplasmic acidophilic hyaline inclusions were also observed in metaplastic mesothelial cells, most of them being placed to the apical cell’s poles. The significance of our findings is discussed in the light of current knowledge regarding the mesothelial cells.

Parole chiave

  • coelomic cavity
  • Emys orbicularis
  • inclusions
  • mesothelial metaplasia
  • Spiroxys
access type Accesso libero

Helminth Parasites of the Three Racerunner Lizards: Eremias pleskei Nikolsky, 1905 (Pleske’s Racerunner-Transcaucasian Racerunner), Eremias strauchi Kessler, 1878 (Strauch’s Racerunner) and Eremias suphani Basoglu & Hellmich, 1968 (Suphan Racerunner) collected from Eastern Part of Turkey

Pubblicato online: 31 Aug 2013
Pagine: 108 - 111

Astratto

Abstract

In this investigation, a total of 44 racerunner lizard samples (Eremias pleskei, Eremias strauchi, and Eremias suphani) collected from eastern part of Turkey were examined for the first time for helminths. Eremias pleskei was harboured 1 species of acanthocepalan (in cystacanth stage), E. strauchi was harboured 2 species of nematodes, and E. suphani was harboured 3 species of nematodes and 1 species of cestode. E. strauchi represents a new host record for Spauligodon eremiasi, and Spauligodon saxicolae E. suphani represents a new host record for Spauligodon eremiasi, S. saxicolae, Physaloptera sp., and Oochoristica tuberculata, and also, E. pleskei represents a new host record for unidentified acanthocepalan (in cystacanth stage). Also, Turkey is a new locality record for Spauligodon eremiasi.

Parole chiave

  • Eremias pleskei
  • Eremias strauchi
  • Eremiassuphani
  • Racerunner lizards
  • Helminth
  • Turkey
access type Accesso libero

A polyphasic approach with molecular phylogeny for the characterization of Anisakis pegreffii (Anisakidae: Nematoda) in fishes from Adriatic Sea

Pubblicato online: 31 Aug 2013
Pagine: 112 - 118

Astratto

Abstract

In this study we investigated the morphometric and molecular characterization of a liver encapsulated third-stage larval population of Anisakis spp. infecting Merluccius merluccius and Lophius piscatorius caught in the Adriatic Sea waters (southern Italy). A polyphasic approach based on PCR-RFLP profiles of the ITS region, mitochondrial COI (cytochrome c oxidase subunit 1), sequencing and molecular phylogeny of ITS and mitochondrial COI was used to identify Anisakis larvae collected from fish samples. PCR-RFLP analysis showed three banding pattern corresponding to the peculiar pattern of A. pegreffii. Sequence data from ribosomal ITS and mitochondrial COI were analysed by Neighbour Joining, Minimum Evolution and Maximum Parsimony methods to evaluate the phylogenetic relationships among A. simplex sensu lato. The phylogenetic trees obtained for both ITS and COI revealed the existence of three distinct clades for A. simplex sensu sricto, A. simplex C and A. pegreffii and the sequences obtained in this study clearly clustered together with A. pegreffii sequences present in the database.

Histopathological observations of anisakid nematode specimens detected on the liver surface of M. merluccius are illustrated. Encapsulated specimens of the L3 stage of the nematode were similar in size and morphometry to those found into the peritoneal cavity. Anisakis larvae encapsulated on the liver surface within dense and pearl coloured envelops caused host hepatic tissue necrosis, large cavities and oedematous liver spots to the host.

Parole chiave

  • Anisakis pegreffii
  • Adriatic Sea
  • mitochondrial COI
  • ITS
  • PCR-RFLP
access type Accesso libero

Ultrastructural characteristics of the vitellarium of Brandesia turgida (Brandes, 1888) (Digenea: Pleurogenidae) and an examination of the potential usefulness of such vitelline traits in digenean systematics

Pubblicato online: 31 Aug 2013
Pagine: 119 - 126

Astratto

Abstract

Transmission electron microscopical observations were made on the vitelline structure of the digenean Brandesia turgida (Brandes, 1888) collected from crypts within the intestinal wall of the frog Pelophylax ridibundus (Pallas, 1771). Ultrastructural details of the vitelline follicles of B. turgida include: (a) the presence within the vitellarium of a single type of cell, i.e. vitellocytes at different stages of their development; (b) a narrow region between the vitellocytes filled with the processes of surrounding parenchymal cells; and (c) the occurrence of the junctional complexes between the vitellocytes and the surrounding parenchymal cells at the periphery of the vitelline follicles. It is shown that the vitelline globules and a few lipid droplets are the main inclusions resulting from vitellocyte synthetic activity. The limited amount of nutritive reserves in the vitellocytes can be explained by the nature of the parasite’s life-cycle, which is characterized by fully-embryonated intrauterine eggs containing a fully-formed miracidium. Despite the small number of digenean species studied in relation to their vitelline cytoarchitecture, two structural patterns of the vitellarium can be elucidated; these are examined in terms of digenean systematic relationships.

Parole chiave

  • Brandesia turgida
  • superfamily Microphalloidea
  • ultrastructure
  • vitellarium
  • digenean families
access type Accesso libero

First report about the trapping activity of Stropharia rugosoannulata acanthocytes for Northern Root Knot Nematode

Pubblicato online: 31 Aug 2013
Pagine: 127 - 131

Astratto

Abstract

This study summarises the results of in vitro screening of the nematophagous activity of Stropharia rugosoannulata and Arthrobotrys oligospora. The tests were conducted with Meloidogyne hapla plant parasitic nematode juveniles placed into Petri dishes containing cultures of the tested fungal species. Immobilisation of the nematodes was observed after 4 and 24 hours. Both species of fungi showed nematophagous activity, however it was much stronger and faster in the case of S. rugosoannulata.

Parole chiave

  • nematophagous fungus
  • Stropharia
  • Arthrobotrys
  • Meloidogyne hapla
  • biocontrol
  • plant parasitic nematodes
access type Accesso libero

In vitro investigations on the biological control of Xiphinema index with Trichoderma species

Pubblicato online: 31 Aug 2013
Pagine: 132 - 137

Astratto

Abstract

The application of Trichoderma spp. for the suppression of plant-parasitic nematode populations is a promising tool in biological control. Sixteen strains of six Trichoderma species (T. atroviride, T. harzianum, T. rossicum, T. tomentosum, T. virens and T. asperellum) were tested in vitro in order to identify the most appropriate strains to control the dagger nematode Xiphinema index. Mortality assays revealed that the strains of the widely investigated T. harzianum species have caused significant reduction of X. index populations, although T. harzianum strains were not the most efficient among all the tested fungi. Certain T. virens and T. atroviride strains and T. rossicum have triggered faster and higher mortality. Generally, our data indicate that Trichoderma species have innate ability to decrease X. index population. Furthermore, as we had difficulties with maintaining X. index in vitro, we successfully used a newly developed method to keep X. index specimens viable during the experiments.

Parole chiave

  • mortality
  • biological control agent
  • dagger nematodes
access type Accesso libero

Gnathostoma binucleatum antigens induce peripheral blood lymphocytic cells proliferation

Pubblicato online: 31 Aug 2013
Pagine: 138 - 141

Astratto

Abstract

Gnathostomiasis is an emerging infectious disease, in which the agent Gnathostoma binucleatum is the only species known to infect humans in Mexico. Although the humoral immune response to G. binucleatum has been analyzed previously, here we evaluated the lymphoproliferative response of peripheral blood lymphocytic cells from patients clinically diagnosed with gnathostomiasis against a crude extract of G. binucleatum using the MTT method, and compared the response to that of healthy subjects. No differences in the response to concanavalin A were found between the groups; however, the response to the crude extract was statistically higher in patients compared to healthy controls (1.188 ± 0.135, and 1.000 ± 0.045, p = 0.0004). Patients at > 6 months after diagnosis showed a higher lymphoproliferative response than the group of healthy subjects, and those at < 6 months after diagnosis (p = 0.0001, and 0.0164 respectively). These data suggest that the parasite induces non-protective immunological memory.

Parole chiave

  • Gnathostomiasis
  • Cellular immune response
  • G. binucleatum
  • non-protective immunity
access type Accesso libero

Unusual clinical course of trichinellosis with relapse

Pubblicato online: 31 Aug 2013
Pagine: 142 - 146

Astratto

Abstract

During an outbreak of trichinellosis in Eastern Slovakia in 2008, 16 people were hospitalized, including 10 patients having specific anti-Trichinella antibodies. The disease relapse was seen in a 68-year-old woman two weeks after her discharge from hospital, where she was treated with albendazole and prednisone for 10 days. Clinical symptoms of trichinellosis, eosinophilia, elevated CRP and LDH levels were observed again with recorded elevation of aminotransferase levels during the relapse. Herein we discuss a potential cause of unusual relapse of trichinellosis with liver damage in a female patient with type II diabetes mellitus.

Parole chiave

  • trichinellosis
  • outbreak
  • symptomatology
  • relapse
12 Articoli
access type Accesso libero

Effect of low temperatures on the infectivity of Toxocara cati larvae parasitized in mouse tissues

Pubblicato online: 31 Aug 2013
Pagine: 83 - 86

Astratto

Abstract

The infectivity of Toxocara cati larvae in mouse tissues, following storage at 4 °C and −25 °C, was measured using a bioassay in mice. Tissues of donor mice infected with 30-day-old T. cati larvae were stored at 4 °C for 7 and 14 days, and at −25 °C for 24 hours, then tissue larvae were inoculated into recipient mice (n = 6/group). After 15 days, larval burden in recipient mice was assessed by digestion. In the control group, a mean of 42.0 % of the inoculated larvae were established in mice. Storage of tissues at 4 °C for 7 and 14 days did not affect larval infectivity; means of 43.5 % and 37.4 % of inoculated larvae, respectively, were established in mice. Larvae exposed to −25 °C for 24 hours did not establish in mice, except for one larva recovered from a single mouse. These results may support the potential of tissue larvae in livestock animals as a causative agent of human toxocarosis.

Parole chiave

  • Toxocara cati
  • tissue larvae
  • cold tolerance
  • zoonosis
access type Accesso libero

Human hydatidosis: an under discussed occupational zoonosis in India

Pubblicato online: 31 Aug 2013
Pagine: 87 - 90

Astratto

Abstract

Human hydatidosis is an important public health issue in India and many other developing countries. There is lack of data related to occurrence of this disease in certain risk groups. The present study was therefore designed to assess the seroprevalence of human hydatidosis among occupational risk groups in Punjab (India). One hundred and forty nine human serum samples from dog handlers, veterinarians/para veterinarians, dairy farmers, patients with liver disorders, HIV positives and control group (visiting hospital for other problems) were collected and analyzed using a commercially available enzyme linked immunosorbent assay (ELISA). The results revealed that 23 (15.43 %) subjects were seropositive for human hydatidosis. High seropositivity were recorded in dog handlers (30 %), followed by the other risk group populations. Relative risk of being infected was found to be 4.80 times high in dog handlers when compared to control group population. Future studies must be planned to determine the significance of these findings and the status of human hydatidosis in India.

Parole chiave

  • seroprevalence
  • human hydatidosis
  • occupational risk groups
  • India
access type Accesso libero

Efficacy of monepantel against lower developmental stages of a multi-resistant and susceptible Haemonchus contortus isolates: an in vitro study

Pubblicato online: 31 Aug 2013
Pagine: 91 - 95

Astratto

Abstract

Monepantel (MOP) belongs to a new class of anthelmintic compounds, the amino-acetonitrile derivates, which have a different mode of action as the currently used anthelmintics. Many present studies confirmed the high efficacy of MOP against fourth larval and adult stages of Haemonchus contortus. The objective of this study was to determine in vitro efficacy of MOP against lower development stages (eggs, L1–L3 larvae) and to compare it between resistant and susceptible isolates of H. contortus. For this purpose, two in vitro tests - egg hatch test and micro-agar larval development test were used. Results were quantified as 50 % lethal concentration (LC50), 99 % lethal concentration (LC99) and resistance factor (RF). This study revealed the high efficacy against lower larval stages (L1–L3) of both resistant and susceptible strains of this parasite. Larval susceptibility was not dependent of the sensitivity status of the nematode isolate. On the other hand, ovicidal effect of MOP was very low.

Parole chiave

  • amino-acetonitrile derivatives
  • antihelmintic resistance
  • micro-agar larval development test
  • monepantel
  • nematode
access type Accesso libero

Cystic echinococcosis in Lombardy: epidemiological aspects and spatial analysis

Pubblicato online: 31 Aug 2013
Pagine: 96 - 103

Astratto

Abstract

A descriptive spatial analysis on the spreading of cattle cystic echinococcosis in non-endemic areas of Italy (Lombardy) was performed and the presence of clusters of hydatid disease in the studied area were evaluated. Positive cattle were from 285 out of 5708 farms (4.99 %) with significant differences among the provinces considered. Infection with Echinococcus granulosus was detected in 321 out of 112,521 dairy cattle (P = 0.29 %). CE-positive farms were distributed among 195 of the 812 municipalities included in the study (24.01 %) located in the central and south-eastern part of the region. By SaTScan, four significant clusters of infection were identified; they included 4–241 cattle farms and 4–24 positive animals. The most likely spatial cluster of 17 observed cases in 241 farms was located in the north-eastern part of Lombardy. SaTScan analysis suggest that a geographical surveillance in the area of the positive clusters occur is for and that’s could be very useful to public health service to monitoring this zoonotic disease.

Parole chiave

  • cattle
  • Echinococcus granulosus
  • Italy
  • spatial analysis
  • cluster
access type Accesso libero

Mesothelial metaplasia in European pond turtle, Emys orbicularis (Testudines: Emydidae) infected with Spiroxys contortus (Nematoda: Spirurida)

Pubblicato online: 31 Aug 2013
Pagine: 104 - 107

Astratto

Abstract

The mesothelium consists of a monolayer of specialized epithelial cells that line the surfaces of the peritoneal, pleural, and pericardial cavities. The present study reports mesothelial changes associated with larval stages of the spirurid nematode Spiroxys contortus in a naturally infected European pond turtle. Between 2002 and 2007, eight European pond turtle, Emys orbicularis were brought for necropsy. In one adult male turtle, necropsy revealed the presence of gastric and mesothelial changes associated with gastric infestation with the nematode Spiroxys contortus. Gastrointestinal samples with gross lesions were collected and processed by paraffin technique for further histological examination. Mesothelial cuboidal to columnar or pseudostratified focal progressive metaplasia was observed in visceral coelomic cavity. Intracytoplasmic acidophilic hyaline inclusions were also observed in metaplastic mesothelial cells, most of them being placed to the apical cell’s poles. The significance of our findings is discussed in the light of current knowledge regarding the mesothelial cells.

Parole chiave

  • coelomic cavity
  • Emys orbicularis
  • inclusions
  • mesothelial metaplasia
  • Spiroxys
access type Accesso libero

Helminth Parasites of the Three Racerunner Lizards: Eremias pleskei Nikolsky, 1905 (Pleske’s Racerunner-Transcaucasian Racerunner), Eremias strauchi Kessler, 1878 (Strauch’s Racerunner) and Eremias suphani Basoglu & Hellmich, 1968 (Suphan Racerunner) collected from Eastern Part of Turkey

Pubblicato online: 31 Aug 2013
Pagine: 108 - 111

Astratto

Abstract

In this investigation, a total of 44 racerunner lizard samples (Eremias pleskei, Eremias strauchi, and Eremias suphani) collected from eastern part of Turkey were examined for the first time for helminths. Eremias pleskei was harboured 1 species of acanthocepalan (in cystacanth stage), E. strauchi was harboured 2 species of nematodes, and E. suphani was harboured 3 species of nematodes and 1 species of cestode. E. strauchi represents a new host record for Spauligodon eremiasi, and Spauligodon saxicolae E. suphani represents a new host record for Spauligodon eremiasi, S. saxicolae, Physaloptera sp., and Oochoristica tuberculata, and also, E. pleskei represents a new host record for unidentified acanthocepalan (in cystacanth stage). Also, Turkey is a new locality record for Spauligodon eremiasi.

Parole chiave

  • Eremias pleskei
  • Eremias strauchi
  • Eremiassuphani
  • Racerunner lizards
  • Helminth
  • Turkey
access type Accesso libero

A polyphasic approach with molecular phylogeny for the characterization of Anisakis pegreffii (Anisakidae: Nematoda) in fishes from Adriatic Sea

Pubblicato online: 31 Aug 2013
Pagine: 112 - 118

Astratto

Abstract

In this study we investigated the morphometric and molecular characterization of a liver encapsulated third-stage larval population of Anisakis spp. infecting Merluccius merluccius and Lophius piscatorius caught in the Adriatic Sea waters (southern Italy). A polyphasic approach based on PCR-RFLP profiles of the ITS region, mitochondrial COI (cytochrome c oxidase subunit 1), sequencing and molecular phylogeny of ITS and mitochondrial COI was used to identify Anisakis larvae collected from fish samples. PCR-RFLP analysis showed three banding pattern corresponding to the peculiar pattern of A. pegreffii. Sequence data from ribosomal ITS and mitochondrial COI were analysed by Neighbour Joining, Minimum Evolution and Maximum Parsimony methods to evaluate the phylogenetic relationships among A. simplex sensu lato. The phylogenetic trees obtained for both ITS and COI revealed the existence of three distinct clades for A. simplex sensu sricto, A. simplex C and A. pegreffii and the sequences obtained in this study clearly clustered together with A. pegreffii sequences present in the database.

Histopathological observations of anisakid nematode specimens detected on the liver surface of M. merluccius are illustrated. Encapsulated specimens of the L3 stage of the nematode were similar in size and morphometry to those found into the peritoneal cavity. Anisakis larvae encapsulated on the liver surface within dense and pearl coloured envelops caused host hepatic tissue necrosis, large cavities and oedematous liver spots to the host.

Parole chiave

  • Anisakis pegreffii
  • Adriatic Sea
  • mitochondrial COI
  • ITS
  • PCR-RFLP
access type Accesso libero

Ultrastructural characteristics of the vitellarium of Brandesia turgida (Brandes, 1888) (Digenea: Pleurogenidae) and an examination of the potential usefulness of such vitelline traits in digenean systematics

Pubblicato online: 31 Aug 2013
Pagine: 119 - 126

Astratto

Abstract

Transmission electron microscopical observations were made on the vitelline structure of the digenean Brandesia turgida (Brandes, 1888) collected from crypts within the intestinal wall of the frog Pelophylax ridibundus (Pallas, 1771). Ultrastructural details of the vitelline follicles of B. turgida include: (a) the presence within the vitellarium of a single type of cell, i.e. vitellocytes at different stages of their development; (b) a narrow region between the vitellocytes filled with the processes of surrounding parenchymal cells; and (c) the occurrence of the junctional complexes between the vitellocytes and the surrounding parenchymal cells at the periphery of the vitelline follicles. It is shown that the vitelline globules and a few lipid droplets are the main inclusions resulting from vitellocyte synthetic activity. The limited amount of nutritive reserves in the vitellocytes can be explained by the nature of the parasite’s life-cycle, which is characterized by fully-embryonated intrauterine eggs containing a fully-formed miracidium. Despite the small number of digenean species studied in relation to their vitelline cytoarchitecture, two structural patterns of the vitellarium can be elucidated; these are examined in terms of digenean systematic relationships.

Parole chiave

  • Brandesia turgida
  • superfamily Microphalloidea
  • ultrastructure
  • vitellarium
  • digenean families
access type Accesso libero

First report about the trapping activity of Stropharia rugosoannulata acanthocytes for Northern Root Knot Nematode

Pubblicato online: 31 Aug 2013
Pagine: 127 - 131

Astratto

Abstract

This study summarises the results of in vitro screening of the nematophagous activity of Stropharia rugosoannulata and Arthrobotrys oligospora. The tests were conducted with Meloidogyne hapla plant parasitic nematode juveniles placed into Petri dishes containing cultures of the tested fungal species. Immobilisation of the nematodes was observed after 4 and 24 hours. Both species of fungi showed nematophagous activity, however it was much stronger and faster in the case of S. rugosoannulata.

Parole chiave

  • nematophagous fungus
  • Stropharia
  • Arthrobotrys
  • Meloidogyne hapla
  • biocontrol
  • plant parasitic nematodes
access type Accesso libero

In vitro investigations on the biological control of Xiphinema index with Trichoderma species

Pubblicato online: 31 Aug 2013
Pagine: 132 - 137

Astratto

Abstract

The application of Trichoderma spp. for the suppression of plant-parasitic nematode populations is a promising tool in biological control. Sixteen strains of six Trichoderma species (T. atroviride, T. harzianum, T. rossicum, T. tomentosum, T. virens and T. asperellum) were tested in vitro in order to identify the most appropriate strains to control the dagger nematode Xiphinema index. Mortality assays revealed that the strains of the widely investigated T. harzianum species have caused significant reduction of X. index populations, although T. harzianum strains were not the most efficient among all the tested fungi. Certain T. virens and T. atroviride strains and T. rossicum have triggered faster and higher mortality. Generally, our data indicate that Trichoderma species have innate ability to decrease X. index population. Furthermore, as we had difficulties with maintaining X. index in vitro, we successfully used a newly developed method to keep X. index specimens viable during the experiments.

Parole chiave

  • mortality
  • biological control agent
  • dagger nematodes
access type Accesso libero

Gnathostoma binucleatum antigens induce peripheral blood lymphocytic cells proliferation

Pubblicato online: 31 Aug 2013
Pagine: 138 - 141

Astratto

Abstract

Gnathostomiasis is an emerging infectious disease, in which the agent Gnathostoma binucleatum is the only species known to infect humans in Mexico. Although the humoral immune response to G. binucleatum has been analyzed previously, here we evaluated the lymphoproliferative response of peripheral blood lymphocytic cells from patients clinically diagnosed with gnathostomiasis against a crude extract of G. binucleatum using the MTT method, and compared the response to that of healthy subjects. No differences in the response to concanavalin A were found between the groups; however, the response to the crude extract was statistically higher in patients compared to healthy controls (1.188 ± 0.135, and 1.000 ± 0.045, p = 0.0004). Patients at > 6 months after diagnosis showed a higher lymphoproliferative response than the group of healthy subjects, and those at < 6 months after diagnosis (p = 0.0001, and 0.0164 respectively). These data suggest that the parasite induces non-protective immunological memory.

Parole chiave

  • Gnathostomiasis
  • Cellular immune response
  • G. binucleatum
  • non-protective immunity
access type Accesso libero

Unusual clinical course of trichinellosis with relapse

Pubblicato online: 31 Aug 2013
Pagine: 142 - 146

Astratto

Abstract

During an outbreak of trichinellosis in Eastern Slovakia in 2008, 16 people were hospitalized, including 10 patients having specific anti-Trichinella antibodies. The disease relapse was seen in a 68-year-old woman two weeks after her discharge from hospital, where she was treated with albendazole and prednisone for 10 days. Clinical symptoms of trichinellosis, eosinophilia, elevated CRP and LDH levels were observed again with recorded elevation of aminotransferase levels during the relapse. Herein we discuss a potential cause of unusual relapse of trichinellosis with liver damage in a female patient with type II diabetes mellitus.

Parole chiave

  • trichinellosis
  • outbreak
  • symptomatology
  • relapse

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