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Volume 54 (2017): Edizione 4 (December 2017)
Special Edizione: This issue is dedicated to the memory of Milan Ryboš, DVM, D.Sc.

Volume 54 (2017): Edizione 3 (September 2017)

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Volume 54 (2017): Edizione 1 (March 2017)

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Volume 47 (2010): Edizione 4 (December 2010)

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Volume 46 (2009): Edizione 4 (December 2009)

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Volume 43 (2006): Edizione 4 (December 2006)

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Volume 43 (2006): Edizione 2 (June 2006)

Volume 43 (2006): Edizione 1 (March 2006)

Dettagli della rivista
Formato
Rivista
eISSN
1336-9083
ISSN
0440-6605
Pubblicato per la prima volta
22 Apr 2006
Periodo di pubblicazione
4 volte all'anno
Lingue
Inglese

Cerca

Volume 43 (2006): Edizione 4 (December 2006)

Dettagli della rivista
Formato
Rivista
eISSN
1336-9083
ISSN
0440-6605
Pubblicato per la prima volta
22 Apr 2006
Periodo di pubblicazione
4 volte all'anno
Lingue
Inglese

Cerca

11 Articoli
Accesso libero

Mesocestoides litteratus (Batsch, 1786) (Cestoda: Cyclophyllidea: Mesocestoididae) from the red fox: Morphological and 18S rDNA characterization of European isolates

Pubblicato online: 01 Dec 2006
Pagine: 191 - 195

Astratto

Abstract

Mesocestoides litteratus (Batsch, 1786) (Cestoda: Cyclophyllidea: Mesocestoidae) is a common parasite of the red fox (Vulpes vulpes) and other carnivores across Europe. There has been considerable debate as to the validity of M. litteratus and other closely related, often sympatric species of Mesocestoides. We examine isolates of M. litteratus from red foxes in the Czech Republic, Slovakia and Spain both morphometrically and by characterization of 18S rDNA. Morphometric ranges of all isolates confirmed their identity as M. litteratus and were usually within the range published formerly. The sequences of 18S rDNA of one or two isolates from each country were analysed. The sequences were the same and distinct from all published Mesocestoides 18S sequences with the exception of tetrathyridia from a lizard in the Czech Republic, which was identical to those of M. litteratus.

Parole chiave

  • Mesocestoides
  • red fox
  • Vulpes vulpes
  • Cestoda
  • morphological analysis
  • DNA
Accesso libero

Genetic structuring and differentiation of Echinococcus multilocularis in Slovakia assessed by sequencing and isoenzyme studies

Pubblicato online: 01 Dec 2006
Pagine: 196 - 202

Astratto

Abstract

Nucleotide sequencing of the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene and isoenzyme analysis were used to survey the genetic variability in Echinococcus multilocularis populations from Slovakia. A sample of 12 isolates acquired from 10 different districts from red foxes exhibited identical sequences. Compared with the previously described E. multilocularis variants, one base substitution was consistently observed relative to the M1 variant (detected in China, Alaska, North America, Japan) and three base substitutions were recorded relative to the M2 variant (detected in Germany) in the CO1 fragment. These data, along with the recently gathered data from French isolates, are indicative of a genetically unique population occurring in Central and Western Europe. Electrophoretic examination of enzymes produced by 14 gene loci revealed intraspecific polymorphism only with the glucose-phosphate isomerase (two distinct patterns) and the mannosephosphate isomerase (four genotypes composed of three alleles) enzyme systems. To allow a fast species differentiation of E. multilocularis and E. granulosus (specifically, the G7 genotype occurring in Slovakia), discriminative electrophoretic characters between the species were obtained by isoenzyme analysis. Fixed genetic differences between the species were detected in the glucose-phosphate isomerase, esterase and aldolase systems, and partial differences were detected in four additional systems.

Parole chiave

  • Echinococcus multilocularis
  • polymorphism
  • DNA
  • isoenzymes
  • Slovakia
Accesso libero

The presence of Syphacia obvelata in laboratory mice (BALB/c) — parasite antigens in immune response

Pubblicato online: 01 Dec 2006
Pagine: 203 - 207

Astratto

Abstract

In conventional mice colonies, mouse pinworm, Syphacia obvelata is found very often. Several studies indicate that infection with this parasite can modulate the immune system of the host and can affect experimental final results. The aim of our study was to investigate the most immunogenic proteins of S. obvelata inducing both local and systemic immune response in naturally infected laboratory mice. Protein extracts of S. obvelata were analysed by Western blotting to examine their antigenic character. The antigens were probed with serum and mucosa of S. obvelata naturally infected mice. Surface and somatic antigens were recognized by serum and mucosal IgG, IgA and IgM antibodies. The most immunogenic and dominant proteins were observed. Proteins of Mw ∼ 70, 65 and 48 kDa showed the most evident reaction with serum and mucosa antibodies of infected animals. Surface and somatic antigens of nematode S. obvelata eliciting immune response in laboratory mice may be useful in development of a diagnostic test which could be applied for the infection control prior the experiments.

Parole chiave

  • Syphacia obvelata
  • pinworms
  • laboratory mice
  • immune response
Accesso libero

The fine structures of Cystocaulus ocreatus (Nematoda: Protostrongylidae) and the related lung pathology

Pubblicato online: 01 Dec 2006
Pagine: 208 - 212

Astratto

Abstract

In the present study, the fine surface structures of Cystocaulus ocreatus and its worm as well as brood nodules obtained from the lung of Akkaraman sheep are demonstrated by scanning electron and light microscopy. The mouth of the parasite obtained from the worm nodules is located at the anterior tip and encircled by a collar of tissue. The mouth opening appears as three slits conjoined in the shape of a capital case letter Y, creating three lips. The mouth is surrounded by six papillae just behind the collar. The anterior end was similar in both sexes and covered by numerous wrinkles. Female parasite has a bell-shaped pro-vagina. The posterior end of the female parasite was sharply pointed. The male parasite has a bursa. In the worm nodule, curled parasites were enclosed by a capsula and no eggs and larvae of the parasite were observed. In the brood nodule, heavily affected alveoli and bronchioles contained excessive amounts of larvae and eggs.

Parole chiave

  • Cystocaulus ocreatus
  • lungworm
  • worm nodule
  • brood nodule
  • scanning electron microscope
Accesso libero

Clinical and serological study of human alveolar echinococcosis in Slovakia in relation to the outcome of chemotherapy

Pubblicato online: 01 Dec 2006
Pagine: 213 - 219

Astratto

Abstract

Alveolar echinococcosis (AE), caused by proliferating metacestodes of the parasitic fox tapeworm Echinococcus multilocularis, is a life-threatening disease in humans. In this study we report four human cases of AE in Slovak Republic with regard to various clinical manifestations and susceptibility to chemotherapy with albendazole or mebendazole. Patients were monitored serologically by ELISA test and Western Blots within 2–5 years after initiation of chemotherapy/surgery. Using computerized tomography (CT) we compared morphological changes of the parasitic lesions in the liver during the course of treatment.

The parasitic lesions in the CT were manifested as nodular hyperplasia with hyper or hypodense zones and calcified foci within the lesion. In other cases the lesion was visualised as a hypodense poorly-delineated septated focus without calcifications. In the last case, the diagnosis of AE was confirmed only at surgery. Calcified foci found inside the main parasitic lesions were the only abnormality that was detected in two cases and were absent in other two cases. The levels of specific anti-parasitic antibodies followed the pathomorphological changes in the livers. Total IgG levels to Em2+ and EmP antigen declined gradually during the follow-up (1–5 years) and disappeared only in the patient receiving radical surgery (cured). In comparison with total IgG, concentration of the IgG4 antibody subclass seemed to correlate more adequately with the outcome of therapy as their levels decreased in improved/stabilised patients, but were elevated in “aggravated” patients. We showed that, in patients with AE in Slovakia, radical surgery of parasitic foci proved to be the most successful treatment and, in inoperable cases, albendazole and mebendazole were differently effective. Moreover. IgG4 levels rather than total IgG to protoscoleces antigens proved to be more sensitive serological marker of the progress of therapy.

Parole chiave

  • Echinococcus multilocularis
  • CT imaging
  • chemotherapy
  • serology
Accesso libero

Comparison of serological and clinical findings in Turkish patients with cystic echinococcosis

Pubblicato online: 01 Dec 2006
Pagine: 220 - 225

Astratto

Abstract

Cystic echinococcosis (CE), caused by the cestode Echinococcus granulosus, is potentially dangerous for humans. The aim of this study was to examine serological and clinical findings regarding cysts localisation and individual responses in 54 patients with CE. The majority of patients in this study were females (63 %) and the average age was 46.3 years. Most of the patients lived in rural areas or kept a dog (46 %) for a long time. The most frequent symptoms were hypochondrial pain (48.9 %), epigastrial discomfort (27.7 %), vomiting (21.3 %), minor cough (12.8 %), urticaria (6.3 %), weakness (4.3 %), fever (2.1 %), side-or back-ache (4.3 %). However, 17 % of the patients showed no symptoms. In every case, the ultrasound (USG) and/or computer tomography (CT) investigations were positive. In most cases (53.2 % of the patients) a single cyst was found but 46.8 % of the patients had multiple cyst formations (from 2 to 9 cysts) located in the liver. Sporadic lung, splenetic, mesenterial, tibial and cerebral localisations were also found. The patients were individually treated with albendazol (10–15 mg/kg) five days prior and six months after the surgical treatment. Serum samples were investigated by the serological techniques: IHAT, ELISA and Western blot using hydatid fluid antigen. In the patient sera, the specific antibody levels were mostly increased after surgery. Different results were obtained only in two patients. In the first case, seroconversion was delayed. In the other case all ELISA results were negative, however, the Western blot analysis and surgery proved the presence of CE. The results suggest that the different antibody response of patients depends on the individual immune response. Multiple localization and various stages of CE cysts demonstrate the necessity of a complex approach for the confirmation of a correct diagnosis.

Parole chiave

  • Echinococcus granulosus
  • cystic echinococcosis
  • clinical finding
  • serodiagnosis
  • Turkey
Accesso libero

Influence of anthelmintic baits on the occurrence of causative agents of helminthozoonoses in red foxes (Vulpes vulpes)

Pubblicato online: 01 Dec 2006
Pagine: 226 - 231

Astratto

Abstract

Red fox (Vulpes vulpes) presents major wildlife reservoir of parasitozoonoses, transmissible to humans and domestic animals. The study was aimed to find out the effect of anthelmintic baits on the occurrence of Echinococcus multilocularis and other intestinal helminths in red foxes. In two bait areas (B1 and B2) 20 baits per km2 were distributed monthly between August 2004 and April 2005. Fox fecal samples were collected in both bait areas and two control areas (C1 and C2) between August 2004 and August 2005. In bait area B1 the decrease of parasite species number, decrease of their prevalence and prevalence of E. multilocularis was observed. No significant decline was observed in bait area B2, probably due to consumption of baits by wild boars. Bait distribution represents the possibility of reduction of environmental contamination with parasites and their propagation stages. Especially on the periphery of towns and villages and in recreational areas it seems to be suitable way of human health protection.

Parole chiave

  • red fox
  • bait
  • praziquantel
  • fenbendazol
  • helminthozoonosis
  • Echinococcus multilocularis
Accesso libero

Long-term variation in an occurrence of Rhabditis orbitalis parasitic larvae (Nematoda, Rhabditidae) in the eyes of montane rodents

Pubblicato online: 01 Dec 2006
Pagine: 232 - 236

Astratto

Abstract

During a long-term survey (1999–2005) of parasitic larvae of nidicolous nematode Rhabditis orbitalis Sudhaus et Schulte, 1986 in the West Tatra Mts, the nematodes were detected in the eye orbits of snow voles Chionomys nivalis in 1999, 2004 and 2005. In the last year, R. orbitalis was detected also in another vole species Clethrionomys glareolus and Microtus tatricus both in previously studied site and in the Low Tatra Mts, however, it has never appeared at other rodent species. For the first time, an irregular occurrence of the R. orbitalis parasitic larvae has been ascertained in natural conditions of the Slovak mountains. The eye nematodes occurred only during autumn or cold and rather wet summer months, when density of the preferred host Ch. nivalis was relatively low. A hypothesis has been put forward that an occasional appearance of parasitic eye larvae in the life cycle of bacteriophagous R. orbitalis represents a strategy for surviving periods of a scarcity of bacterial food, which could be influenced by a combination of weather conditions, density of host rodents and seasonal abundance of R. orbitalis in rodent nests.

Parole chiave

  • Rhabditis orbitalis
  • Nematoda
  • Rodentia
  • Tatra Mountains
Accesso libero

First report of Syphacia vanderbrueli Bernard, 1961 (Oxyuridae) from Micromys minutus in Poland

Pubblicato online: 01 Dec 2006
Pagine: 237 - 238

Astratto

Abstract

During the parasitological examination of wild rodents from Wroclaw vicinity 11 female nematodes were isolated from the harvest mouse (Micromys minutus). The helminths were determined as Syphacia vanderbrueli. This is the first report of this parasite in Poland. Complete descriptions of the female individuals are given.

Parole chiave

  • Syphacia vanderbrueli
  • rodents
  • Poland
Accesso libero

First record of Diplotriaena henryi Blanc, 1919 from the coal tit, Parus ater with new report from the great tit, Parus major in the Middle East

Pubblicato online: 01 Dec 2006
Pagine: 239 - 241

Astratto

Abstract

Seven males and thirteen female Diplotriaena henryi Blanc 1919 were collected from body cavities (heart, sternum, and chest) of tits (one Parus major and three Parus ater) in plain woodland of Noor, in Northern Iran.

Parole chiave

  • Diplotriaena henryi
  • Parus major
  • Parus ater
  • Iran
Accesso libero

The first report of authochthonous dirofilariosis in dogs in the Czech Republic

Pubblicato online: 01 Dec 2006
Pagine: 242 - 245

Astratto

Abstract

In the Czech Republic, canine dirofilarial infection (Dirofilaria immitis and D. repens) is usually diagnosed in dogs coming from endemic areas and as such has been considered an imported infection. Here, 77 dogs that had never travelled abroad from the Břeclav area, close to Slovak border, were tested for Dirofilaria spp. infection. The presence of microfilaria in peripheral blood was detected by Knott test. Microfilariae were further examined by acid phosphatase staining and molecular methods (PCR). The presence of adult female D. immitis circulating antigens in blood was assessed by a commercial kit (PetChek, IDEXX Laboratories, Portland, USA). Microfilariae were detected in 7 (9 %) out of the 77 animals by the Knott test. The result of the acid phosphatase staining and PCR for all seven samples agreed with Dirofilaria repens species. Other five dogs of the 77 sera (6.5 %) sampled were serologically positive for circulating D. immitis antigens. No D. immitis microfilariae were found in these five dogs. D. repens positive dogs were negative on the ELISA for D. immitis. This is the first report of autochthonous cases of heart-worm disease and subcutaneous dirofilariosis in dogs in the Czech Republic.

Parole chiave

  • Dirofilaria repens
  • Dirofilaria immitis
  • dog
  • autochthonous infection
11 Articoli
Accesso libero

Mesocestoides litteratus (Batsch, 1786) (Cestoda: Cyclophyllidea: Mesocestoididae) from the red fox: Morphological and 18S rDNA characterization of European isolates

Pubblicato online: 01 Dec 2006
Pagine: 191 - 195

Astratto

Abstract

Mesocestoides litteratus (Batsch, 1786) (Cestoda: Cyclophyllidea: Mesocestoidae) is a common parasite of the red fox (Vulpes vulpes) and other carnivores across Europe. There has been considerable debate as to the validity of M. litteratus and other closely related, often sympatric species of Mesocestoides. We examine isolates of M. litteratus from red foxes in the Czech Republic, Slovakia and Spain both morphometrically and by characterization of 18S rDNA. Morphometric ranges of all isolates confirmed their identity as M. litteratus and were usually within the range published formerly. The sequences of 18S rDNA of one or two isolates from each country were analysed. The sequences were the same and distinct from all published Mesocestoides 18S sequences with the exception of tetrathyridia from a lizard in the Czech Republic, which was identical to those of M. litteratus.

Parole chiave

  • Mesocestoides
  • red fox
  • Vulpes vulpes
  • Cestoda
  • morphological analysis
  • DNA
Accesso libero

Genetic structuring and differentiation of Echinococcus multilocularis in Slovakia assessed by sequencing and isoenzyme studies

Pubblicato online: 01 Dec 2006
Pagine: 196 - 202

Astratto

Abstract

Nucleotide sequencing of the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene and isoenzyme analysis were used to survey the genetic variability in Echinococcus multilocularis populations from Slovakia. A sample of 12 isolates acquired from 10 different districts from red foxes exhibited identical sequences. Compared with the previously described E. multilocularis variants, one base substitution was consistently observed relative to the M1 variant (detected in China, Alaska, North America, Japan) and three base substitutions were recorded relative to the M2 variant (detected in Germany) in the CO1 fragment. These data, along with the recently gathered data from French isolates, are indicative of a genetically unique population occurring in Central and Western Europe. Electrophoretic examination of enzymes produced by 14 gene loci revealed intraspecific polymorphism only with the glucose-phosphate isomerase (two distinct patterns) and the mannosephosphate isomerase (four genotypes composed of three alleles) enzyme systems. To allow a fast species differentiation of E. multilocularis and E. granulosus (specifically, the G7 genotype occurring in Slovakia), discriminative electrophoretic characters between the species were obtained by isoenzyme analysis. Fixed genetic differences between the species were detected in the glucose-phosphate isomerase, esterase and aldolase systems, and partial differences were detected in four additional systems.

Parole chiave

  • Echinococcus multilocularis
  • polymorphism
  • DNA
  • isoenzymes
  • Slovakia
Accesso libero

The presence of Syphacia obvelata in laboratory mice (BALB/c) — parasite antigens in immune response

Pubblicato online: 01 Dec 2006
Pagine: 203 - 207

Astratto

Abstract

In conventional mice colonies, mouse pinworm, Syphacia obvelata is found very often. Several studies indicate that infection with this parasite can modulate the immune system of the host and can affect experimental final results. The aim of our study was to investigate the most immunogenic proteins of S. obvelata inducing both local and systemic immune response in naturally infected laboratory mice. Protein extracts of S. obvelata were analysed by Western blotting to examine their antigenic character. The antigens were probed with serum and mucosa of S. obvelata naturally infected mice. Surface and somatic antigens were recognized by serum and mucosal IgG, IgA and IgM antibodies. The most immunogenic and dominant proteins were observed. Proteins of Mw ∼ 70, 65 and 48 kDa showed the most evident reaction with serum and mucosa antibodies of infected animals. Surface and somatic antigens of nematode S. obvelata eliciting immune response in laboratory mice may be useful in development of a diagnostic test which could be applied for the infection control prior the experiments.

Parole chiave

  • Syphacia obvelata
  • pinworms
  • laboratory mice
  • immune response
Accesso libero

The fine structures of Cystocaulus ocreatus (Nematoda: Protostrongylidae) and the related lung pathology

Pubblicato online: 01 Dec 2006
Pagine: 208 - 212

Astratto

Abstract

In the present study, the fine surface structures of Cystocaulus ocreatus and its worm as well as brood nodules obtained from the lung of Akkaraman sheep are demonstrated by scanning electron and light microscopy. The mouth of the parasite obtained from the worm nodules is located at the anterior tip and encircled by a collar of tissue. The mouth opening appears as three slits conjoined in the shape of a capital case letter Y, creating three lips. The mouth is surrounded by six papillae just behind the collar. The anterior end was similar in both sexes and covered by numerous wrinkles. Female parasite has a bell-shaped pro-vagina. The posterior end of the female parasite was sharply pointed. The male parasite has a bursa. In the worm nodule, curled parasites were enclosed by a capsula and no eggs and larvae of the parasite were observed. In the brood nodule, heavily affected alveoli and bronchioles contained excessive amounts of larvae and eggs.

Parole chiave

  • Cystocaulus ocreatus
  • lungworm
  • worm nodule
  • brood nodule
  • scanning electron microscope
Accesso libero

Clinical and serological study of human alveolar echinococcosis in Slovakia in relation to the outcome of chemotherapy

Pubblicato online: 01 Dec 2006
Pagine: 213 - 219

Astratto

Abstract

Alveolar echinococcosis (AE), caused by proliferating metacestodes of the parasitic fox tapeworm Echinococcus multilocularis, is a life-threatening disease in humans. In this study we report four human cases of AE in Slovak Republic with regard to various clinical manifestations and susceptibility to chemotherapy with albendazole or mebendazole. Patients were monitored serologically by ELISA test and Western Blots within 2–5 years after initiation of chemotherapy/surgery. Using computerized tomography (CT) we compared morphological changes of the parasitic lesions in the liver during the course of treatment.

The parasitic lesions in the CT were manifested as nodular hyperplasia with hyper or hypodense zones and calcified foci within the lesion. In other cases the lesion was visualised as a hypodense poorly-delineated septated focus without calcifications. In the last case, the diagnosis of AE was confirmed only at surgery. Calcified foci found inside the main parasitic lesions were the only abnormality that was detected in two cases and were absent in other two cases. The levels of specific anti-parasitic antibodies followed the pathomorphological changes in the livers. Total IgG levels to Em2+ and EmP antigen declined gradually during the follow-up (1–5 years) and disappeared only in the patient receiving radical surgery (cured). In comparison with total IgG, concentration of the IgG4 antibody subclass seemed to correlate more adequately with the outcome of therapy as their levels decreased in improved/stabilised patients, but were elevated in “aggravated” patients. We showed that, in patients with AE in Slovakia, radical surgery of parasitic foci proved to be the most successful treatment and, in inoperable cases, albendazole and mebendazole were differently effective. Moreover. IgG4 levels rather than total IgG to protoscoleces antigens proved to be more sensitive serological marker of the progress of therapy.

Parole chiave

  • Echinococcus multilocularis
  • CT imaging
  • chemotherapy
  • serology
Accesso libero

Comparison of serological and clinical findings in Turkish patients with cystic echinococcosis

Pubblicato online: 01 Dec 2006
Pagine: 220 - 225

Astratto

Abstract

Cystic echinococcosis (CE), caused by the cestode Echinococcus granulosus, is potentially dangerous for humans. The aim of this study was to examine serological and clinical findings regarding cysts localisation and individual responses in 54 patients with CE. The majority of patients in this study were females (63 %) and the average age was 46.3 years. Most of the patients lived in rural areas or kept a dog (46 %) for a long time. The most frequent symptoms were hypochondrial pain (48.9 %), epigastrial discomfort (27.7 %), vomiting (21.3 %), minor cough (12.8 %), urticaria (6.3 %), weakness (4.3 %), fever (2.1 %), side-or back-ache (4.3 %). However, 17 % of the patients showed no symptoms. In every case, the ultrasound (USG) and/or computer tomography (CT) investigations were positive. In most cases (53.2 % of the patients) a single cyst was found but 46.8 % of the patients had multiple cyst formations (from 2 to 9 cysts) located in the liver. Sporadic lung, splenetic, mesenterial, tibial and cerebral localisations were also found. The patients were individually treated with albendazol (10–15 mg/kg) five days prior and six months after the surgical treatment. Serum samples were investigated by the serological techniques: IHAT, ELISA and Western blot using hydatid fluid antigen. In the patient sera, the specific antibody levels were mostly increased after surgery. Different results were obtained only in two patients. In the first case, seroconversion was delayed. In the other case all ELISA results were negative, however, the Western blot analysis and surgery proved the presence of CE. The results suggest that the different antibody response of patients depends on the individual immune response. Multiple localization and various stages of CE cysts demonstrate the necessity of a complex approach for the confirmation of a correct diagnosis.

Parole chiave

  • Echinococcus granulosus
  • cystic echinococcosis
  • clinical finding
  • serodiagnosis
  • Turkey
Accesso libero

Influence of anthelmintic baits on the occurrence of causative agents of helminthozoonoses in red foxes (Vulpes vulpes)

Pubblicato online: 01 Dec 2006
Pagine: 226 - 231

Astratto

Abstract

Red fox (Vulpes vulpes) presents major wildlife reservoir of parasitozoonoses, transmissible to humans and domestic animals. The study was aimed to find out the effect of anthelmintic baits on the occurrence of Echinococcus multilocularis and other intestinal helminths in red foxes. In two bait areas (B1 and B2) 20 baits per km2 were distributed monthly between August 2004 and April 2005. Fox fecal samples were collected in both bait areas and two control areas (C1 and C2) between August 2004 and August 2005. In bait area B1 the decrease of parasite species number, decrease of their prevalence and prevalence of E. multilocularis was observed. No significant decline was observed in bait area B2, probably due to consumption of baits by wild boars. Bait distribution represents the possibility of reduction of environmental contamination with parasites and their propagation stages. Especially on the periphery of towns and villages and in recreational areas it seems to be suitable way of human health protection.

Parole chiave

  • red fox
  • bait
  • praziquantel
  • fenbendazol
  • helminthozoonosis
  • Echinococcus multilocularis
Accesso libero

Long-term variation in an occurrence of Rhabditis orbitalis parasitic larvae (Nematoda, Rhabditidae) in the eyes of montane rodents

Pubblicato online: 01 Dec 2006
Pagine: 232 - 236

Astratto

Abstract

During a long-term survey (1999–2005) of parasitic larvae of nidicolous nematode Rhabditis orbitalis Sudhaus et Schulte, 1986 in the West Tatra Mts, the nematodes were detected in the eye orbits of snow voles Chionomys nivalis in 1999, 2004 and 2005. In the last year, R. orbitalis was detected also in another vole species Clethrionomys glareolus and Microtus tatricus both in previously studied site and in the Low Tatra Mts, however, it has never appeared at other rodent species. For the first time, an irregular occurrence of the R. orbitalis parasitic larvae has been ascertained in natural conditions of the Slovak mountains. The eye nematodes occurred only during autumn or cold and rather wet summer months, when density of the preferred host Ch. nivalis was relatively low. A hypothesis has been put forward that an occasional appearance of parasitic eye larvae in the life cycle of bacteriophagous R. orbitalis represents a strategy for surviving periods of a scarcity of bacterial food, which could be influenced by a combination of weather conditions, density of host rodents and seasonal abundance of R. orbitalis in rodent nests.

Parole chiave

  • Rhabditis orbitalis
  • Nematoda
  • Rodentia
  • Tatra Mountains
Accesso libero

First report of Syphacia vanderbrueli Bernard, 1961 (Oxyuridae) from Micromys minutus in Poland

Pubblicato online: 01 Dec 2006
Pagine: 237 - 238

Astratto

Abstract

During the parasitological examination of wild rodents from Wroclaw vicinity 11 female nematodes were isolated from the harvest mouse (Micromys minutus). The helminths were determined as Syphacia vanderbrueli. This is the first report of this parasite in Poland. Complete descriptions of the female individuals are given.

Parole chiave

  • Syphacia vanderbrueli
  • rodents
  • Poland
Accesso libero

First record of Diplotriaena henryi Blanc, 1919 from the coal tit, Parus ater with new report from the great tit, Parus major in the Middle East

Pubblicato online: 01 Dec 2006
Pagine: 239 - 241

Astratto

Abstract

Seven males and thirteen female Diplotriaena henryi Blanc 1919 were collected from body cavities (heart, sternum, and chest) of tits (one Parus major and three Parus ater) in plain woodland of Noor, in Northern Iran.

Parole chiave

  • Diplotriaena henryi
  • Parus major
  • Parus ater
  • Iran
Accesso libero

The first report of authochthonous dirofilariosis in dogs in the Czech Republic

Pubblicato online: 01 Dec 2006
Pagine: 242 - 245

Astratto

Abstract

In the Czech Republic, canine dirofilarial infection (Dirofilaria immitis and D. repens) is usually diagnosed in dogs coming from endemic areas and as such has been considered an imported infection. Here, 77 dogs that had never travelled abroad from the Břeclav area, close to Slovak border, were tested for Dirofilaria spp. infection. The presence of microfilaria in peripheral blood was detected by Knott test. Microfilariae were further examined by acid phosphatase staining and molecular methods (PCR). The presence of adult female D. immitis circulating antigens in blood was assessed by a commercial kit (PetChek, IDEXX Laboratories, Portland, USA). Microfilariae were detected in 7 (9 %) out of the 77 animals by the Knott test. The result of the acid phosphatase staining and PCR for all seven samples agreed with Dirofilaria repens species. Other five dogs of the 77 sera (6.5 %) sampled were serologically positive for circulating D. immitis antigens. No D. immitis microfilariae were found in these five dogs. D. repens positive dogs were negative on the ELISA for D. immitis. This is the first report of autochthonous cases of heart-worm disease and subcutaneous dirofilariosis in dogs in the Czech Republic.

Parole chiave

  • Dirofilaria repens
  • Dirofilaria immitis
  • dog
  • autochthonous infection

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