- Journal Details
- Format
- Journal
- eISSN
- 2719-9509
- First Published
- 01 Jan 1992
- Publication timeframe
- 4 times per year
- Languages
- English
Search
- Open Access
Synthesis, Purification, and Quantification of Fatty Acid Ethyl Esters After trans-Esterification of Large Batches of Tobacco Seed Oil
Page range: 205 - 213
Abstract
The goal of the study was to quantify fatty acid ethyl esters (FAEE’s) produced from two large batches of tobacco seed oil after trans-esterification by heating in ethanol with sulfuric acid catalyst. Purification of the combined ethyl ester reaction products by removing as much of the color and odor from the final product as possible was achieved via conventional column chromatography with amorphous silica and tandem elution of first hexane and then ethyl alcohol as the mobile phase. Gas chromatography was used to quantify specific FAEE’s in the purified material. Recovery of pure FAEE's in batch #1 was near 87%; while, recovery of FAEE’s in batch #2 was greater than 89% with mass yields greater than 400 g of ethyl esters per esterification trial. The FAEE’s possessed no detectable aroma and only a slight yellow color after this chromatographic treatment. Supercritical fluid chromatography with a mobile phase of methanol/acetonitrile modified carbon dioxide and an octadecyl bonded silica stationary phase were used to characterize the purity of each batch of fatty acid ethyl ester product. No free fatty acids nor glycerolrelated impurities were detected in the purified transesterified product.This is the first report describing the optimized trans-esterification of tobacco seed oil on a relatively large scale coupled with subsequent purification and isolation of the resultant ethyl esters. [Beitr. Tabakforsch. Int. 26 (2015) 205-213]
- Open Access
Measurement of Chlorinated Dioxins and Furans in Cigarette Mainstream Smoke
Page range: 214 - 218
Abstract
Chlorinated dioxins and furans (‘dioxins’) are not ordinarily detected in cigarette smoke at levels likely to exceed the recommended ‘safe’ limits recognised by health and environmental protection agencies. By convention they are measured in terms of their total toxic equivalence (TEQ). The method developed by this group utilises high sensitivity gas chromatography-tandem mass spectrometry (GCMS/ MS). The limit of detection (LOD) was determined to be 4.3 pg TEQ/cig; under ISO 3308 smoking conditions. Dioxins are not subject to any routine regulatory control in cigarette smoke. However, certain dioxin congeners are highly toxic and they have been cited by the United States Food and Drug Administration (FDA) as harmful and potentially harmful constituents (HPHC) of tobacco smoke. This raises the issue of how to monitor and measure uncertainty when the analyte(s) of interest are not normally present in the matrix of interest. In the absence of a reference cigarette containing levels of dioxins above the detection limit; we prepared test pieces containing known quantities of 17 dioxin congeners, regarded by the World Health Organisation (WHO) as significantly toxic. This paper provides data for the transfer rates performed under ISO 3308 (ISO) and Health Canada Intense (HCI) machinesmoking conditions; and proposes a means to estimate the uncertainty (U) based on TEQ. [Beitr. Tabakforsch. Int. 26 (2015) 214-218]
- Open Access
Development and Characterisation of a Smoking Behaviour Measurement System
Page range: 219 - 231
Abstract
Respiratory deposition from smoking articles is influenced by particle or droplet properties (such as diameter), puffing parameters (such as draw effort or pressure drop, flow rate, duration), mouth losses, and post-puff inhalation dynamics (determined by inhalation depth, volume and breath hold duration). The Smoking Behaviour System (SBS) described herein is a novel system with regard to its capability to measure flow and duration characteristics of typical smoking cycles, which may consist of puffing, mouth hold, post-puff inhalation, breath hold and exhalation. It combines two analysers: the first measures the puffing topography and optical obscuration from puffed aerosol, while the second measures the respiratory topography and optical obscuration from exhaled aerosol. The puffing and respiratory analysers were calibrated and operated between flow rates of 0-7 L.min-1 and 0-50 L.min-1, respectively, as typically encountered during the human smoking cycle. The volumes measured by the puffing and respiratory analysers met the design specification and tolerance limits of ± 2% and ± 5%, respectively. The optical obscuration measurements showed no statistical difference among the three units tested or in day-to-day variation, verifying the robustness of the SBS for use in optical measurements of smoke when combined with topography measurements. In conclusion, the SBS is a validated technology that provides a means to measure the real-time flow and duration profile of puffing, mouth hold, post-puff inhalation, breath hold
Keywords
- Smoking behaviour system
- puffing topography
- respiratory topography
- optical obscuration
- Open Access
An Improved Theoretical Model of Cigarette Smoke Filtration across Mono-Segment Cellulose Acetate Filters
Page range: 232 - 240
Abstract
An improved theoretical model was presented to predict the filtration efficiency of cigarette filters. Filtration equations of single fibers considering the interference of neighboring fibers were applied in the model. Cellulose acetate fibers in cigarette filters were approximated as cylinders. The fiber size was adjusted by its size projected on the flow field. The solid fraction of fibers in cigarette filters was recalculated using the size of the virtual cylinders. The varying flow velocity during smoking was taken into account when calculating the filtration efficiency. The effective hydrodynamic particle diameter of cigarette smoke was estimated to be 0.44 μm by the difference of filtration efficiencies under ISO and Health Canada Intense (HCI) smoking regimes. Filtration contributions due to diffusion, interception and inertial impaction were 62%, 32% and 6%, respectively, at a flow velocity of 0.38 m/s for particles of 0.44 μm diameter. The effect of inertial impaction was insignificant but not negligible under ISO smoking regime. The measured and predicted efficiencies of two cigarette samples were compared and satisfactory agreement was obtained. [Beitr. Tabakforsch. Int. 26 (2015) 232-240]