Published Online: 28 May 2014 Page range: 155 - 164
Abstract
Abstract
For the estimation of the different methods used for obtaining and fractioning tobacco tars, it is necessary to consider the reactivity of the For the estimation of the different methods used for obtaining and fractioning tobacco tars, it is necessary to consider the reactivity of the individual components and the characteristic action of the entire product. For analytical and biological tests a product is required which has not undergone any important chemical or biochemical changes. It is shown that this important condition has mostly been neglected. The changes likely to occur in the tar during the different smoking and preparation procedures are discussed. It is pointed out that a great number of compounds have been described, in particular in the more recent literature, which are eager to react with other substances and which, being constituents of tobacco smoke, might be responsible for such chemical changes.Several tars were submitted to a limited ageing under different physical conditions. Such simple tests demonstrate the important changes taking place in the original tar when submitted to temperature, light and air and if concentrations in the solvent are too high. On the basis of such general reflections it is concluded that the biological tests described until now require reexamination and that it is necessary to develop and apply such procedures, both for the production and the preparation of tobacco tars, which prevent the formation of artefacts
Published Online: 28 May 2014 Page range: 165 - 169
Abstract
SUMMARY
In our opinion mechanical smoke collection traps do not make sufficient allowance for the particular chemical and physico chemical properties of tobacco tar and its constituents, and, therefore, do not produce the tar in its original state. For this reason a new apparatus has been designed which compared to other processes makes it possible to collect the tobacco smoke in a solvent. Our experience has shown that this is the only practicable method of producing a tobacco tar which as far as possible remains unchanged by chemical reactions and is suitable for biological tests. The tobacco smoke is washed out by the solvent in a wash bottle and a suitably diluted tar solution is obtained which is drawn off intermittently. The specially designed smoking apparatus is separately mounted on top so that the smoke passes into the wash bottle by the shortest possible route. The entire machine is built into a framework of angle iron and is mounted on vibration dampers to eliminate oscillation. It enables a large number of cigarettes to be smoked in a very short time
Published Online: 28 May 2014 Page range: 177 - 179
Abstract
Abstract
Microscopic examinations suggest that the trichomes, owing to their dew condensing qualities, when moist, and to their stickiness, when dry, are responsible for the trapping and fixing of the conidia. They can therefore be regarded to be the basis of the epidemic appearance of Peronospora tabacina Adam
Published Online: 28 May 2014 Page range: 180 - 186
Abstract
Abstract
Following an introductory survey of the literature concerning the induction of bronchial cancer in laboratory animals after exposure to cigarette smoke or intrapulmonary application of smoke condensates, a method is described for a single pertracheal instillation of smoke condensate into peripheral areas of the bronchial system of rats. Histological examination of the lungs showed the appearance of intrapulmonary foreign body granulomas and squamous cell metaplasia of the bronchial epithelium. According to the experiences of oncology, the lack of neoplastic reactions in spite of the 10 months’ duration of the experiments might be explained by the small dosages which had to be limited due to the applied technique. As far as we know at present, the possible maximum carcinogenic potency of the smoke condensates applied is only about 1/100 of those dosages of pure carcinogens which, according to the literature, are sufficient to cause bronchial carcinoma in rats. For technical reasons it seems recommendable for further in vivo studies with lung tissue to use concentrated tobacco smoke condensate or its fractions.
Published Online: 28 May 2014 Page range: 187 - 191
Abstract
Abstract
A smoking machine has been built which, while being as simple as possible is designed to carry out almost every conceivable function that might be required of a laboratory smoking machine and which allows the operator complete freedom of choice on every aspect of his smoking conditions
Published Online: 28 May 2014 Page range: 193 - 194
Abstract
Abstract
An apparatus for automatic weight selection of cigarettes or filter rods has been constructed, which is specially suited for the preparation of working samples for chemical and physical tests
Published Online: 28 May 2014 Page range: 195 - 198
Abstract
Abstract
4. Vorschrift für die Bestimmung des Nikotins im Tabak, im Tabakrauchkondensat und in Filtern 4.1 Bestimmung des Nikotins im Tabak 4.2 Bestimmung des Nikotins im Tabakrauchkondensat 4.3 Bestimmung des Nikotins in Cigarettenfiltern
For the estimation of the different methods used for obtaining and fractioning tobacco tars, it is necessary to consider the reactivity of the For the estimation of the different methods used for obtaining and fractioning tobacco tars, it is necessary to consider the reactivity of the individual components and the characteristic action of the entire product. For analytical and biological tests a product is required which has not undergone any important chemical or biochemical changes. It is shown that this important condition has mostly been neglected. The changes likely to occur in the tar during the different smoking and preparation procedures are discussed. It is pointed out that a great number of compounds have been described, in particular in the more recent literature, which are eager to react with other substances and which, being constituents of tobacco smoke, might be responsible for such chemical changes.Several tars were submitted to a limited ageing under different physical conditions. Such simple tests demonstrate the important changes taking place in the original tar when submitted to temperature, light and air and if concentrations in the solvent are too high. On the basis of such general reflections it is concluded that the biological tests described until now require reexamination and that it is necessary to develop and apply such procedures, both for the production and the preparation of tobacco tars, which prevent the formation of artefacts
In our opinion mechanical smoke collection traps do not make sufficient allowance for the particular chemical and physico chemical properties of tobacco tar and its constituents, and, therefore, do not produce the tar in its original state. For this reason a new apparatus has been designed which compared to other processes makes it possible to collect the tobacco smoke in a solvent. Our experience has shown that this is the only practicable method of producing a tobacco tar which as far as possible remains unchanged by chemical reactions and is suitable for biological tests. The tobacco smoke is washed out by the solvent in a wash bottle and a suitably diluted tar solution is obtained which is drawn off intermittently. The specially designed smoking apparatus is separately mounted on top so that the smoke passes into the wash bottle by the shortest possible route. The entire machine is built into a framework of angle iron and is mounted on vibration dampers to eliminate oscillation. It enables a large number of cigarettes to be smoked in a very short time
Microscopic examinations suggest that the trichomes, owing to their dew condensing qualities, when moist, and to their stickiness, when dry, are responsible for the trapping and fixing of the conidia. They can therefore be regarded to be the basis of the epidemic appearance of Peronospora tabacina Adam
Following an introductory survey of the literature concerning the induction of bronchial cancer in laboratory animals after exposure to cigarette smoke or intrapulmonary application of smoke condensates, a method is described for a single pertracheal instillation of smoke condensate into peripheral areas of the bronchial system of rats. Histological examination of the lungs showed the appearance of intrapulmonary foreign body granulomas and squamous cell metaplasia of the bronchial epithelium. According to the experiences of oncology, the lack of neoplastic reactions in spite of the 10 months’ duration of the experiments might be explained by the small dosages which had to be limited due to the applied technique. As far as we know at present, the possible maximum carcinogenic potency of the smoke condensates applied is only about 1/100 of those dosages of pure carcinogens which, according to the literature, are sufficient to cause bronchial carcinoma in rats. For technical reasons it seems recommendable for further in vivo studies with lung tissue to use concentrated tobacco smoke condensate or its fractions.
A smoking machine has been built which, while being as simple as possible is designed to carry out almost every conceivable function that might be required of a laboratory smoking machine and which allows the operator complete freedom of choice on every aspect of his smoking conditions
An apparatus for automatic weight selection of cigarettes or filter rods has been constructed, which is specially suited for the preparation of working samples for chemical and physical tests
4. Vorschrift für die Bestimmung des Nikotins im Tabak, im Tabakrauchkondensat und in Filtern 4.1 Bestimmung des Nikotins im Tabak 4.2 Bestimmung des Nikotins im Tabakrauchkondensat 4.3 Bestimmung des Nikotins in Cigarettenfiltern