<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Lactose intolerance is an important factor restricting the consumption of dairy products. Lactase is used to hydrolyze lactose in milk while generating galactooligosaccharides (GOS), thereby reducing the incidence of lactose intolerance. We used cow milk as raw materials, and selected enzyme preparations with high lactose hydrolysis rate and strong GOS generation ability from 14 commercially available lactase enzymes. The lactose hydrolysis rate is 5.85%-81.38%, and the GOS content is 0.03 g/L- 13.10 g/L. The mixing experiment design determined the two lactase enzymes (E10 and E11) ratio and the optimal enzymatic hydrolysis process of low-lactose prebiotic milk: compound lactases (E10:E11=0.756:0.244) addition 0.11%, 55℃for 5h, lactose hydrolysis rate and GOS content were 98.02% and 19.69g/L, respectively, and the remaining lactose content was about 0.97 g/L.</p>
</abstract>

Lactose intolerance is an important factor restricting the consumption of dairy products. Lactase is used to hydrolyze lactose in milk while generating galactooligosaccharides (GOS), thereby reducing the incidence of lactose intolerance. We used cow milk as raw materials, and selected enzyme preparations with high lactose hydrolysis rate and strong GOS generation ability from 14 commercially available lactase enzymes. The lactose hydrolysis rate is 5.85%-81.38%, and the GOS content is 0.03 g/L- 13.10 g/L. The mixing experiment design determined the two lactase enzymes (E10 and E11) ratio and the optimal enzymatic hydrolysis process of low-lactose prebiotic milk: compound lactases (E10:E11=0.756:0.244) addition 0.11%, 55℃for 5h, lactose hydrolysis rate and GOS content were 98.02% and 19.69g/L, respectively, and the remaining lactose content was about 0.97 g/L.

Screening of lactases suitable for the preparation of low-lactose prebiotic liquid milk and optimisation of their combination

Shaanxi University of Science & Technology

Acta Universitatis Cibiniensis. Series E: Food Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Lactose intolerance is an important factor restricting the consumption of dairy products. Lactase is used to hydrolyze lactose in milk while generating...

Screening of lactases suitable for the preparation of low-lactose prebiotic liquid milk and optimisation of their combination

Published Online: Dec 30, 2021

Page range: 275 - 284

Received: Oct 03, 2021

Accepted: Dec 12, 2021

DOI: https://doi.org/10.2478/aucft-2021-0025

Keywords
Lactase, cow milk, enzymatic hydrolysis, galacto-oligosaccharide, prebiotics

© 2020 Yunli Xu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Screening of lactases suitable for the preparation of low-lactose prebiotic liquid milk and optimisation of their combination

Published Online: Dec 30, 2021

Page range: 275 - 284

Received: Oct 03, 2021

Accepted: Dec 12, 2021

DOI: https://doi.org/10.2478/aucft-2021-0025

KeywordsLactase, cow milk, enzymatic hydrolysis, galacto-oligosaccharide, prebiotics

© 2020 Yunli Xu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
Lactase, cow milk, enzymatic hydrolysis, galacto-oligosaccharide, prebiotics