<abstract xmlns="http://www.w3.org/1999/xhtml">Two glucose-limited realkalized fed-batch cultures of <italic>Lactococcus lactis</italic> CECT 539 were carried out in a diluted whey medium (DW) using two different feeding media. The cultures were fed a mixture of a 400 g/l concentrated lactose and a concentrated mussel processing waste (CMPW, 101.72 g glucose/l) medium (fermentation I) or a CMPW medium supplemented with glucose and KH2PO4 up to concentrations of 400 g glucose/l and 3.21 g total phosphorus/l, respectively (fermentation II). For an accurate description and a better understanding of the kinetics of both cultures, the growth and product formation by <italic>L. lactis</italic> CECT 539 were both modelled, for the first time, as a function of the amounts of glucose (<italic>G</italic>) added and the pH gradient (<italic>VpH</italic>) generated in every realkalization and feeding cycle, by using an empirical polynomial model. With this modeling procedure, the kinetics of biomass, viable cell counts, nisin, lactic acid, acetic acid and butane-2,3-diol production in both cultures were successfully described (<italic>R</italic>2 values > 0.970) and interpreted for the first time. In addition, the optimum <italic>VpH</italic> and <italic>G</italic> values for each product were accurately calculated in the two realkalized fed-batch cultures. This approach appears to be useful for designing feeding strategies to enhance the productions of biomass, bacteriocin, and metabolites by the nisin-producing strain in wastes from the food industry.</abstract>

Two glucose-limited realkalized fed-batch cultures of Lactococcus lactis CECT 539 were carried out in a diluted whey medium (DW) using two different feeding media. The cultures were fed a mixture of a 400 g/l concentrated lactose and a concentrated mussel processing waste (CMPW, 101.72 g glucose/l) medium (fermentation I) or a CMPW medium supplemented with glucose and KH2PO4 up to concentrations of 400 g glucose/l and 3.21 g total phosphorus/l, respectively (fermentation II). For an accurate description and a better understanding of the kinetics of both cultures, the growth and product formation by L. lactis CECT 539 were both modelled, for the first time, as a function of the amounts of glucose (G) added and the pH gradient (VpH) generated in every realkalization and feeding cycle, by using an empirical polynomial model. With this modeling procedure, the kinetics of biomass, viable cell counts, nisin, lactic acid, acetic acid and butane-2,3-diol production in both cultures were successfully described (R2 values > 0.970) and interpreted for the first time. In addition, the optimum VpH and G values for each product were accurately calculated in the two realkalized fed-batch cultures. This approach appears to be useful for designing feeding strategies to enhance the productions of biomass, bacteriocin, and metabolites by the nisin-producing strain in wastes from the food industry.

The Joint Effect of pH Gradient and Glucose Feeding on the Growth Kinetics of <italic>Lactococcus lactis</italic> CECT 539 in Glucose-Limited Fed-Batch Cultures

Polish Journal of Microbiology

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The Joint Effect of pH Gradient and Glucose Feeding on the Growth Kinetics of  CECT 539 in Glucose-Limited Fed-Batch Cultures

Two glucose-limited realkalized fed-batch cultures of Lactococcus lactis CECT 539 were carried out in a diluted whey medium (DW) using two different feeding...

The Joint Effect of pH Gradient and Glucose Feeding on the Growth Kinetics of Lactococcus lactis CECT 539 in Glucose-Limited Fed-Batch Cultures

Article Category: original-paper

Published Online: Jun 28, 2019

Page range: 269 - 280

Received: Feb 24, 2019

Accepted: Apr 25, 2019

DOI: https://doi.org/10.33073/pjm-2019-030

Keywords
fed-batch fermentation, empirical modeling, probiotic biomass, nisin, glucose-limited cultures

© 2019 Mónica Costas Malvido et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The Joint Effect of pH Gradient and Glucose Feeding on the Growth Kinetics of Lactococcus lactis CECT 539 in Glucose-Limited Fed-Batch Cultures

Article Category: original-paper

Published Online: Jun 28, 2019

Page range: 269 - 280

Received: Feb 24, 2019

Accepted: Apr 25, 2019

DOI: https://doi.org/10.33073/pjm-2019-030

Keywordsfed-batch fermentation, empirical modeling, probiotic biomass, nisin, glucose-limited cultures

© 2019 Mónica Costas Malvido et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
fed-batch fermentation, empirical modeling, probiotic biomass, nisin, glucose-limited cultures