Most of the phosphorus (P) in feed ingredients is present as phytate, which is poorly available for absorption in the gastrointestinal tract of different poultry species due to the lack of endogenous phytase. The supplementation of phytase increases the utilization of P by hydrolyzing phytate, which consequently may reduce the excretion of P in the environment. In addition, it has been suggested that phytase may improve the feed utilization, weight gain, egg production and egg traits, nutrient digestibility, energy availability, retention of important minerals in blood and bones. Thus, the effectiveness of phytase on performance and Ca and P absorption in layer chickens fed corn-soybean based diets has been well recognized. The current review briefly discusses the supplementation of phytase in the diet of poultry on performance and egg production and characteristics as well as amino acids and minerals availability.