Threonine (Thr) is the third limiting essential amino acid after methionine and lysine in cornsoybean based diets of broilers. Dietary imbalance of Thr, therefore, results in a poor growth performance in broilers. This review summarizes literature data on the known effects of dietary levels of Thr on growth performance, gut morphology, immunity and carcass characteristics in broilers. Due to continuous improvement in genetic potential and management practices for poultry production, dietary Thr requirements are changing. A number of studies have shown that supplementation of Thr in broiler diet at a higher level than the current NRC recommendation (0.74-0.81%), increases body weight gain, feed conversion ratio, and improves gut morphology, carcass quality and immune status, mainly by enhancing the functional capability of digestive system and immune organs (spleen, bursa, and thymus). According to the literature data discussed in this review, the minimal and maximal total dietary Thr levels for healthy birds reared in normal conditions were 0.67 and 0.90% for growth performance, 0.77 and 1.1% for a better gut health, 0.60 and 1.02% for immunity and 0.62 and 0.97% for better carcass characteristics. This background provides impetus to further investigate the exact level of Thr and its effects on growth performance, gut morphology, immunity and carcass characteristics in broilers.