First report of the isolation and molecular characterisation of Lactococcus garvieae in dairy cattle in Poland
Henryk Krukowski
Department of Animal Hygiene and Environmental HazardsLublin, Poland
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, Andrzej Lisowski
Department of Cattle Breeding and Genetic Resources Conservation, University of Life Sciences in LublinLublin, Poland
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, Elżbieta Puacz
Maria Sklodowska-Curie Warsaw Higher School of MedicineWarsaw, Poland
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, Witold Chabuz
Department of Cattle Breeding and Genetic Resources Conservation, University of Life Sciences in LublinLublin, Poland
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oraz Małgorzata Targońska-Karasek
Department of Animal Hygiene and Environmental HazardsLublin, Poland
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13 cze 2025
O artykule
Data publikacji: 13 cze 2025
Zakres stron: 213 - 218
Otrzymano: 29 sty 2025
Przyjęty: 05 cze 2025
DOI: https://doi.org/10.2478/jvetres-2025-0035
Słowa kluczowe
© 2025 Henryk Krukowski et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Antimicrobial agents used in the study and antimicrobial susceptibilities of Lactococcus garvieae
| Antimicrobial agent | MIC range (mg/L) | |||||
|---|---|---|---|---|---|---|
| Strain No. 5 | Strain No. 13 | Strain No. 64 | ||||
| MIC | Interpretation | MIC | Interpretation | MIC | Interpretation | |
| Benzylpenicillin | 0.5 | R | 1 | R | 0.5 | R |
| Clindamycin | ≤ 1 | R | ≤1 | R | ≤1 | R |
| Linezolid | ≤2 | S | ≤2 | S | ≤2 | S |
| Teicoplanin | ≤0.12 | S | ≤0.12 | S | ≤0.12 | S |
| Vancomycin | 0.5 | S | 0.5 | S | 0.5 | S |
| Tetracycline | 0.5 | S | ≤16 | R | 0.5 | S |
| Tigecycline | ≤0.06 | S | ≤0.06 | S | ≤0.06 | S |
| Moxifloxacin | 0.5 | S | 0.5 | S | 0.5 | S |
| Chloramphenicol | 4 | S | 2 | S | 4 | S |
| Rifampicin | ≤4 | R | ≤4 | R | ≤4 | R |
| Erythromycin | ≤0.12 | S | ≤0.12 | S | ≤0.12 | S |
| Trimethoprim-sulfamethoxazole | ≤0.12 | S | ≤10 | S | ≤10 | S |
Results of basic local alignment search tool analysis
| Bacterial strain No. | Query coverage (%) | Identity (%) | GenBank sequence length (base pairs) | Species | Accession No. |
|---|---|---|---|---|---|
| 5 | 95 | 98.74 | 1,471 | LC377166.1 | |
| 13 | 91 | 97.21 | 1,233 | PP422542.1 | |
| 64 | 88 | 85.16 | 895 | MT000064.1 |
Groups of microorganisms identified in microbiological analyses
| Isolates | Number (n) | % of all milk samples | % of all strains |
|---|---|---|---|
| 2 | 1.69 | 4.88 | |
| 1 | 0.85 | 2.44 | |
| 10 | 8.47 | 24.39 | |
| 3 | 2.54 | 7.32 | |
| 1 | 0.85 | 2.44 | |
| Coagulase-negative staphylococci | 18 | 15.25 | 43.90 |
| Coagulase-positive staphylococci | 5 | 4.24 | 12.20 |
| 1 | 0.85 | 2.44 | |
| Contamination | 1 | 0.85 | – |
| No growth | 76 | 64.41 | – |
| Total | 118 | 100 | 100 |