First report of the isolation and molecular characterisation of Lactococcus garvieae in dairy cattle in Poland
Henryk Krukowski
Department of Animal Hygiene and Environmental HazardsLublin, Poland
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, Andrzej Lisowski
Department of Cattle Breeding and Genetic Resources Conservation, University of Life Sciences in LublinLublin, Poland
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, Elżbieta Puacz
Maria Sklodowska-Curie Warsaw Higher School of MedicineWarsaw, Poland
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, Witold Chabuz
Department of Cattle Breeding and Genetic Resources Conservation, University of Life Sciences in LublinLublin, Poland
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y Małgorzata Targońska-Karasek
Department of Animal Hygiene and Environmental HazardsLublin, Poland
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13 jun 2025
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Publicado en línea: 13 jun 2025
Páginas: 213 - 218
Recibido: 29 ene 2025
Aceptado: 05 jun 2025
DOI: https://doi.org/10.2478/jvetres-2025-0035
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© 2025 Henryk Krukowski et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Antimicrobial agents used in the study and antimicrobial susceptibilities of Lactococcus garvieae
| Antimicrobial agent | MIC range (mg/L) | |||||
|---|---|---|---|---|---|---|
| Strain No. 5 | Strain No. 13 | Strain No. 64 | ||||
| MIC | Interpretation | MIC | Interpretation | MIC | Interpretation | |
| Benzylpenicillin | 0.5 | R | 1 | R | 0.5 | R |
| Clindamycin | ≤ 1 | R | ≤1 | R | ≤1 | R |
| Linezolid | ≤2 | S | ≤2 | S | ≤2 | S |
| Teicoplanin | ≤0.12 | S | ≤0.12 | S | ≤0.12 | S |
| Vancomycin | 0.5 | S | 0.5 | S | 0.5 | S |
| Tetracycline | 0.5 | S | ≤16 | R | 0.5 | S |
| Tigecycline | ≤0.06 | S | ≤0.06 | S | ≤0.06 | S |
| Moxifloxacin | 0.5 | S | 0.5 | S | 0.5 | S |
| Chloramphenicol | 4 | S | 2 | S | 4 | S |
| Rifampicin | ≤4 | R | ≤4 | R | ≤4 | R |
| Erythromycin | ≤0.12 | S | ≤0.12 | S | ≤0.12 | S |
| Trimethoprim-sulfamethoxazole | ≤0.12 | S | ≤10 | S | ≤10 | S |
Results of basic local alignment search tool analysis
| Bacterial strain No. | Query coverage (%) | Identity (%) | GenBank sequence length (base pairs) | Species | Accession No. |
|---|---|---|---|---|---|
| 5 | 95 | 98.74 | 1,471 | LC377166.1 | |
| 13 | 91 | 97.21 | 1,233 | PP422542.1 | |
| 64 | 88 | 85.16 | 895 | MT000064.1 |
Groups of microorganisms identified in microbiological analyses
| Isolates | Number (n) | % of all milk samples | % of all strains |
|---|---|---|---|
| 2 | 1.69 | 4.88 | |
| 1 | 0.85 | 2.44 | |
| 10 | 8.47 | 24.39 | |
| 3 | 2.54 | 7.32 | |
| 1 | 0.85 | 2.44 | |
| Coagulase-negative staphylococci | 18 | 15.25 | 43.90 |
| Coagulase-positive staphylococci | 5 | 4.24 | 12.20 |
| 1 | 0.85 | 2.44 | |
| Contamination | 1 | 0.85 | – |
| No growth | 76 | 64.41 | – |
| Total | 118 | 100 | 100 |