Rivista e Edizione

Volume 30 (2022): Edizione 3 (July 2022)

Volume 30 (2022): Edizione 2 (April 2022)

Volume 30 (2022): Edizione 1 (January 2022)

Volume 29 (2021): Edizione 4 (October 2021)

Volume 29 (2021): Edizione 3 (July 2021)

Volume 29 (2021): Edizione 2 (April 2021)

Volume 29 (2021): Edizione 1 (January 2021)

Volume 28 (2020): Edizione 4 (October 2020)

Volume 28 (2020): Edizione 3 (July 2020)

Volume 28 (2020): Edizione 2 (April 2020)

Volume 28 (2020): Edizione 1 (January 2020)

Volume 27 (2019): Edizione 4 (October 2019)

Volume 27 (2019): Edizione 3 (July 2019)

Volume 27 (2019): Edizione 2 (April 2019)

Volume 27 (2019): Edizione 1 (January 2019)

Volume 26 (2018): Edizione 4 (October 2018)

Volume 26 (2018): Edizione 3 (July 2018)

Volume 26 (2018): Edizione 2 (April 2018)

Volume 26 (2018): Edizione 1 (January 2018)

Volume 25 (2017): Edizione 4 (October 2017)

Volume 25 (2017): Edizione 3 (July 2017)

Volume 25 (2017): Edizione 2 (April 2017)

Volume 25 (2017): Edizione 1 (January 2017)

Volume 24 (2016): Edizione 4 (December 2016)

Volume 24 (2016): Edizione 3 (September 2016)

Volume 24 (2016): Edizione 2 (June 2016)

Volume 24 (2016): Edizione 1 (March 2016)

Volume 23 (2015): Edizione 4 (December 2015)

Volume 23 (2015): Edizione 3 (August 2015)

Volume 23 (2015): Edizione 2 (June 2015)

Volume 23 (2015): Edizione 1 (March 2015)

Volume 22 (2014): Edizione 4 (December 2014)

Volume 22 (2014): Edizione 3 (September 2014)

Volume 22 (2014): Edizione 2 (June 2014)

Volume 22 (2014): Edizione 1 (March 2014)

Volume 21 (2013): Edizione 4 (December 2013)

Volume 21 (2013): Edizione 3 (September 2013)

Volume 21 (2013): Edizione 2 (June 2013)

Volume 21 (2013): Edizione 1 (March 2013)

Dettagli della rivista
Formato
Rivista
eISSN
2284-5623
Pubblicato per la prima volta
08 Aug 2013
Periodo di pubblicazione
4 volte all'anno
Lingue
Inglese

Cerca

Volume 30 (2022): Edizione 1 (January 2022)

Dettagli della rivista
Formato
Rivista
eISSN
2284-5623
Pubblicato per la prima volta
08 Aug 2013
Periodo di pubblicazione
4 volte all'anno
Lingue
Inglese

Cerca

12 Articoli
Accesso libero

The Effect of Resveratrol on Sphingosine-1 and Oxidative/ Nitrosative Stress in an Experimental Heart Ischemia Reperfusion Model

Pubblicato online: 12 Feb 2022
Pagine: 9 - 18

Astratto

Abstract

Objectives: Resveratrol (RSV) is a natural polyphenolic compound showing significant antioxidant effects. In this study, we aimed to investigate the effects of resveratrol on the sphingosine-1-phosphate (S1P) and oxidative stress biomarkers in hearth ischemia-reperfusion (I/R).

Materials and Methods: The biochemical and histopathological effects of RSV on cardiac ischemia-reperfusion injury were investigated through ELISA- and light microscope.

Results: We observed statistically significant differences between the treatment group and the control group in terms of malondialdehyde (MDA) level, catalase (CAT) and superoxide dismutase (SOD) activities (p<0.05). Histopathologically, we also observed decreased Polymorphonuclear Leucocyte (PMNL) infiltration, myocardial edema, miyositolysis in the treatment group compared to the I/R and sham groups.

Conclusion: Resveratrol may play an important role in cardiac I/R injury through its anti-inflammatory and antioxidant effects which were biochemically and histopathologically confirmed in the present study.

Parole chiave

  • resveratrol
  • ischemia/reperfusion injury
  • antioxidant
  • oxidative stress
  • heart
Accesso libero

Effects of lactoferrin on osteogenic differentiation and related gene expressions of osteoblast precursor cells MC3T3-E1 under mechanical strain

Pubblicato online: 12 Feb 2022
Pagine: 19 - 30

Astratto

Abstract

We aimed to evaluate the effects of lactoferrin (LF) on the osteogenic differentiation and related gene expressions of mouse embryonic osteoblast precursor cells MC3T3-E1 under mechanical strain. MC3T3-E1 cells were randomly divided into control, strain loading, LF and strain loading + LF groups. Alkaline phosphatase (ALP) activity was measured. Cytoskeletal morphology was measured by rhodamine-phalloidin staining. Formation of mineralized nodules was observed by alizarin red staining. The expressions of differentiation-related genes type I collagen (COL-1), interleukin-6 (IL-6) and osteocalcin (OCN) were determined by RT-PCR, and those of p-Runx2 and p-ERK1/2 proteins were detected by Western blotting. The number of ALP positive cells and expressions of OCN, COL-1 and IL-6 were significantly elevated (P<0.05). The optical density of strain loading + LF group was higher than those in strain loading and LF groups after incubation for 4 and 7 days (P<0.05). The cell volume and extension range were elevated in strain loading + LF group compared with those in strain loading group. The amount of mineralized nodules in strain loading + LF group was significantly higher than those in strain loading and LF groups, while it was slightly higher in LF group than that in strain loading group. The expressions of p-ERK1/2 and p-Runx2 in strain loading + LF group exceeded those in strain loading and LF groups (P<0.05). The synergistic action of LF and mechanical strain can effectively promote the proliferation, differentiation and mineralization of osteoblasts, probably being associated with the ERK1/2 signaling pathway.

Parole chiave

  • lactoferrin
  • mechanical strain
  • MC3T3-E1
  • proliferation
  • differentiation
Accesso libero

Association of C5a/C5aR pathway to activate ERK1/2 and p38 MAPK in acute kidney injury – a mouse model

Pubblicato online: 12 Feb 2022
Pagine: 31 - 40

Astratto

Abstract

Acute inflammation is accompanied by complement system activation and inflammatory cell accumulation. Acute kidney injury (AKI) is one of the common clinical symptoms, it is not clear whether complement system-mediated signaling pathway is involved. This study demonstrated that the expressions of complement C5a and C5a receptor (C5aR) protein in a mouse model with glycerol induced AKI were significantly increased, and the expression of inflammatory cytokines, such as IL-1β, IL-6 and TNF-α, were significantly higher than those in the blank control group. While C5aR antagonist (C5aRa) was added, western analyses for C5a and C5aR were reduced, meanwhile, qPCR and ELISA data showed that inflammatory cytokines also decreased significantly. In addition, preliminarily explored, the Mitogen-activated protein kinases (MAPKs) can be activated by the C5a/C5aR pathway in an AKI mouse model which showed that the C5a/C5aR pathway in a mouse model group activated ERK1/2 and p38, and the protein expression decreased when C5aRa was added. In conclusion, these results indicate that the C5a/C5aR pathway promotes renal pathogenesis by activating ERK1/2 and p38 expression and then affects the disease process, which has certain guiding significance for the subsequent clinical trial.

Parole chiave

  • Acute kidney injury
  • C5a/C5aR
  • MAPK
Accesso libero

Expressions and clinical significance of interleukin-1β and cyclooxygenase-2 in serum and synovia of patients with gouty arthritis

Pubblicato online: 12 Feb 2022
Pagine: 41 - 48

Astratto

Abstract

Background: To explore the expressions and clinical significance of interleukin-1β (IL-1β) and cyclooxygenase-2 (COX-2) in serum and synovia of patients with gouty arthritis (GA).

Materials and Methods: A total of 110 GA patients hospitalized from January 2016 to September 2019 were selected as observation group, with 55 at remission stage (observation group I) and 55 at acute attack stage (observation group II). Another 55 healthy volunteers were selected as control group. The correlations of IL-1β and COX-2 with uric acid (UA) were explored by Spearman’s analysis. IL-1β and COX-2 expression levels were compared at different time points after treatment.

Results: IL-1β, COX-2 and UA levels of patients were significantly higher in observation group I and II than those in the control group, and higher in the observation group II than those in observation group I. IL-1β and COX-2 were positively correlated with UA (P<0.05). ROC curve analysis showed that the optimal cut-off values of IL-1β and COX-2 for predicting GA were 18.23 and 9.12 pg/mL, and AUC values were 0.702 and 0.714 (P<0.001), respectively. On admission and after treatment for 3, 7, and 10 days, IL-1β and COX-2 levels of observation group II significantly exceeded those of observation group I (P<0.05). Compared with the efficacy group, after treatment for 14 days, IL-1β and COX-2 levels in serum and synovia significantly increased in the non-efficacy group (P<0.05).

Conclusion: IL-1β and COX-2 levels in serum and synovia of GA patients are evidently higher than those in the control group, which rise with the aggravation of disease.

Parole chiave

  • Interleukin-1β
  • Cyclooxygenase-2
  • Gouty arthritis
Accesso libero

An insight into the fecal microbiota composition in Romanian patients with ankylosing spondylitis using high-throughput 16S rRNA gene amplicon sequencing

Pubblicato online: 12 Feb 2022
Pagine: 49 - 61

Astratto

Abstract

Introduction. Application of next-generation sequencing technology generated a massive amount of information on the gut microbiome composition used to understand its role in the healthy state and in various diseases. We aimed to provide information on the gut microbiota composition of Romanian subjects diagnosed with ankylosing spondylitis, an immune-mediated arthropathy linked to a genetic predisposition and gut dysbiosis.

Methods. Stool samples collected from 25 patients with ankylosing spondylitis and 16 healthy controls were investigated using high-throughput DNA sequencing of 16S rRNA amplicons from seven different hypervariable regions and Ion Torrent PGM instrument. Microbial composition of metagenomic data was analyzed with QIIME software and differential abundance analysis of taxa encompassed linear discriminant analysis effect size (LEfSe).

Results. Overall, 14 phyla, 114 families, 114 genera, and 275 species were identified across the 41 samples, the aggregated data revealing as most abundant the phyla Bacteroidetes, Firmicutes, and Proteobacteria, the families Bacteroidaceae, Prevotellaceae, and Ruminococcaceae, the genera Bacteroides, Prevotella, and Faecalibacterium, and Prevotella copri species. Using various cutoffs for abundance and prevalence, core taxonomic members were identified which in general were shared between the patients and controls. However, evidence was gained that the diversity in the microbiomes from the former cohort was lower than for controls and that certain taxa had significantly different abundance between the two groups.

Conclusion. This study allowed an informative high-throughput 16S rRNA profiling of the gut microbiota needed to identify microbiome signatures of risk in the autochthonous population with AS.

Parole chiave

  • ankylosing spondylitis
  • next generation sequencing
  • gut microbiome
  • 16S rRNA amplicon sequencing
Accesso libero

The role of neutrophil-to-lymphocyte ratio as an outcome predictor factor in acute appendicitis: a single medical center’s experience

Pubblicato online: 12 Feb 2022
Pagine: 63 - 70

Astratto

Abstract

Introduction: Acute abdominal pain persists in defying modern diagnostic procedures. Inflammatory response variables in urine and blood tests can help rule out other disorders and support a clinical diagnosis of acute appendicitis (AA), but there is no specific blood test that can diagnose AA. In order to address this, our study sought to establish the value of (NLR) as a prognostic and severity predictor in patients with AA.

Material and method: We conducted an observational retrospective study of 356 patients who had undergone surgery for AA, assessing NLR as an outcome metric. The NLR was estimated using the differential WBC (white blood count) reported on admission; the data obtained were then recorded in a database and statistically analyzed.

Results: The NLR medians correlated (p<0.001) in uncomplicated AA, AA with localized peritonitis, and AA with generalized peritonitis (Kruskal-Wallis test). The ROC curve identified 81.4 % sensitivity, 62.5 % specificity, and a 73 % AUC in localized peritonitis. The NLR value was associated with generalized peritonitis with a sensitivity of 63.7 %, specificity of 64.3 %, and AUC of 68.2%. The NLR-patient death association had 100% sensitivity, 80.3% specificity, and 93.5 AUC.

Conclusion: NLR determination in individuals with acute appendicitis may be useful in predicting complications. NLR values greater than 7.86 are usually related with generalized peritonitis, while values larger than 12.9 may predict an increased risk of patient death. Calculating NLR from a complete blood count is a straightforward and cost-effective method of analyzing complicated AA in resource-constrained settings.

Parole chiave

  • appendicitis
  • inflammation
  • neutrophil-to-lymphocyte ratio
Accesso libero

Values of PCT and STREM-1 combined with clinical pulmonary infection score for the prognostic evaluation of elderly patients with ventilator-associated pneumonia

Pubblicato online: 12 Feb 2022
Pagine: 71 - 79

Astratto

Abstract

Objective: To explore the values of PCT and STREM-1 combined with clinical pulmonary infection score (CPIS) for evaluating ventilator-associated pneumonia (VAP) in elderly patients.

Methods: A total of 120 eligible patients were selected as VAP group, and 60 other patients without VAP were selected as control group. According to APACHE II scores, VAP group was divided into low-, medium-, and high-risk groups. The correlations of PCT, STREM-1, and CPIS with APACHE II score were analyzed. The values of PCT and STREM-1 combined with CPIS for evaluating prognosis were analyzed using ROC curves.

Results: In the VAP group, serum PCT and STREM-1 levels and CPIS after mechanical ventilation significantly exceeded those of the control group and before mechanical ventilation (P<0.05). These values were higher in the high-risk group than in medium- and low-risk groups (P<0.05). They were positively correlated with APACHE II score (P<0.001). These values of the death group were significantly higher than those of the survival group 1, 3, 5, and 7 d after treatment (P<0.05). AUC of PCT and STREM-1 combined with CPIS for assessing prognosis was 0.892 (P<0.001).

Conclusions: Serum PCT and STREM-1 levels and CPIS significantly increase in elderly patients with VAP, which rise with aggravation. Their combination has higher values for prognostic evaluation.

Parole chiave

  • procalcitonin
  • soluble triggering receptor expressed on myeloid cells-1
  • clinical pulmonary infection score
  • ventilator-associated pneumonia
Accesso libero

Expression of tight junction transmembrane protein Claudin-1 in gastric carcinoma and effects on tumor cell proliferation, invasion and migration

Pubblicato online: 12 Feb 2022
Pagine: 81 - 91

Astratto

Abstract

Background: Claudin-1 is involved in various cancers, but its expression and role in gastric carcinoma remain unclear.

Materials and Methods: Gastric carcinoma and adjacent normal tissues were harvested from 60 patients. Claudin-1 expression was detected by RT-qPCR. The expressions in human gastric carcinoma MKN45, SGC7901 and MKN28 cells and immortalized human gastric epithelium GES-1 cells were determined by RT-qPCR and Western blotting. Claudin-1 was overexpressed in SGC7901 cells by lentiviral transfection, and they were divided into Control (untransfected), normal control (NC) (transfected with lentiviral vector) and Claudin-1 (transfected with Claudin-1 overexpression lentivirus) groups. The proliferation, invasion and migration of gastric carcinoma cells were detected through cell counting kit-8, Transwell and wound healing assays, respectively. The effects of Claudin-1 on the expressions of epithelial-mesenchymal transition (EMT) marker proteins E-cadherin and N-cadherin were detected by Western blotting. Ten 4-week-old male BALB/c nude mice were subcutaneously injected with lentivirus-treated SGC7901 cells to establish the transplanted tumor model, and the effect of overexpression of Claudin-1 was explored.

Results: The expression of Claudin-1 in gastric carcinoma tissues was significantly lower than that in adjacent tissues (P<0.05). Overexpression of Claudin-1 significantly inhibited the proliferation, invasion and migration of SGC7901 cells, increased the expression of E-cadherin, and decreased that of N-cadherin (P<0.05). Overexpression of Claudin-1 in the mouse model significantly inhibited the growth of subcutaneous transplanted tumors (P<0.05).

Conclusion: Claudin-1 has low expression in gastric carcinoma tissues. Overexpression of Claudin-1 inhibits the proliferation, invasion, migration, and EMT of gastric carcinoma cells, and subcutaneous tumorigenesis in nude mice.

Parole chiave

  • proliferation
  • gastric cancer
  • invasion
  • Claudin-1
  • epithelial-mesenchymal transition
Accesso libero

Letter to the Editor

Pubblicato online: 12 Feb 2022
Pagine: 93 - 95

Astratto

Accesso libero

Evaluation of iron replacement therapy efficiency using a complex panel of biomarkers

Pubblicato online: 12 Feb 2022
Pagine: 97 - 105

Astratto

Accesso libero

Correction

Pubblicato online: 12 Feb 2022
Pagine: 107 - 107

Astratto

Accesso libero

Correction

Pubblicato online: 12 Feb 2022
Pagine: 108 - 108

Astratto

12 Articoli
Accesso libero

The Effect of Resveratrol on Sphingosine-1 and Oxidative/ Nitrosative Stress in an Experimental Heart Ischemia Reperfusion Model

Pubblicato online: 12 Feb 2022
Pagine: 9 - 18

Astratto

Abstract

Objectives: Resveratrol (RSV) is a natural polyphenolic compound showing significant antioxidant effects. In this study, we aimed to investigate the effects of resveratrol on the sphingosine-1-phosphate (S1P) and oxidative stress biomarkers in hearth ischemia-reperfusion (I/R).

Materials and Methods: The biochemical and histopathological effects of RSV on cardiac ischemia-reperfusion injury were investigated through ELISA- and light microscope.

Results: We observed statistically significant differences between the treatment group and the control group in terms of malondialdehyde (MDA) level, catalase (CAT) and superoxide dismutase (SOD) activities (p<0.05). Histopathologically, we also observed decreased Polymorphonuclear Leucocyte (PMNL) infiltration, myocardial edema, miyositolysis in the treatment group compared to the I/R and sham groups.

Conclusion: Resveratrol may play an important role in cardiac I/R injury through its anti-inflammatory and antioxidant effects which were biochemically and histopathologically confirmed in the present study.

Parole chiave

  • resveratrol
  • ischemia/reperfusion injury
  • antioxidant
  • oxidative stress
  • heart
Accesso libero

Effects of lactoferrin on osteogenic differentiation and related gene expressions of osteoblast precursor cells MC3T3-E1 under mechanical strain

Pubblicato online: 12 Feb 2022
Pagine: 19 - 30

Astratto

Abstract

We aimed to evaluate the effects of lactoferrin (LF) on the osteogenic differentiation and related gene expressions of mouse embryonic osteoblast precursor cells MC3T3-E1 under mechanical strain. MC3T3-E1 cells were randomly divided into control, strain loading, LF and strain loading + LF groups. Alkaline phosphatase (ALP) activity was measured. Cytoskeletal morphology was measured by rhodamine-phalloidin staining. Formation of mineralized nodules was observed by alizarin red staining. The expressions of differentiation-related genes type I collagen (COL-1), interleukin-6 (IL-6) and osteocalcin (OCN) were determined by RT-PCR, and those of p-Runx2 and p-ERK1/2 proteins were detected by Western blotting. The number of ALP positive cells and expressions of OCN, COL-1 and IL-6 were significantly elevated (P<0.05). The optical density of strain loading + LF group was higher than those in strain loading and LF groups after incubation for 4 and 7 days (P<0.05). The cell volume and extension range were elevated in strain loading + LF group compared with those in strain loading group. The amount of mineralized nodules in strain loading + LF group was significantly higher than those in strain loading and LF groups, while it was slightly higher in LF group than that in strain loading group. The expressions of p-ERK1/2 and p-Runx2 in strain loading + LF group exceeded those in strain loading and LF groups (P<0.05). The synergistic action of LF and mechanical strain can effectively promote the proliferation, differentiation and mineralization of osteoblasts, probably being associated with the ERK1/2 signaling pathway.

Parole chiave

  • lactoferrin
  • mechanical strain
  • MC3T3-E1
  • proliferation
  • differentiation
Accesso libero

Association of C5a/C5aR pathway to activate ERK1/2 and p38 MAPK in acute kidney injury – a mouse model

Pubblicato online: 12 Feb 2022
Pagine: 31 - 40

Astratto

Abstract

Acute inflammation is accompanied by complement system activation and inflammatory cell accumulation. Acute kidney injury (AKI) is one of the common clinical symptoms, it is not clear whether complement system-mediated signaling pathway is involved. This study demonstrated that the expressions of complement C5a and C5a receptor (C5aR) protein in a mouse model with glycerol induced AKI were significantly increased, and the expression of inflammatory cytokines, such as IL-1β, IL-6 and TNF-α, were significantly higher than those in the blank control group. While C5aR antagonist (C5aRa) was added, western analyses for C5a and C5aR were reduced, meanwhile, qPCR and ELISA data showed that inflammatory cytokines also decreased significantly. In addition, preliminarily explored, the Mitogen-activated protein kinases (MAPKs) can be activated by the C5a/C5aR pathway in an AKI mouse model which showed that the C5a/C5aR pathway in a mouse model group activated ERK1/2 and p38, and the protein expression decreased when C5aRa was added. In conclusion, these results indicate that the C5a/C5aR pathway promotes renal pathogenesis by activating ERK1/2 and p38 expression and then affects the disease process, which has certain guiding significance for the subsequent clinical trial.

Parole chiave

  • Acute kidney injury
  • C5a/C5aR
  • MAPK
Accesso libero

Expressions and clinical significance of interleukin-1β and cyclooxygenase-2 in serum and synovia of patients with gouty arthritis

Pubblicato online: 12 Feb 2022
Pagine: 41 - 48

Astratto

Abstract

Background: To explore the expressions and clinical significance of interleukin-1β (IL-1β) and cyclooxygenase-2 (COX-2) in serum and synovia of patients with gouty arthritis (GA).

Materials and Methods: A total of 110 GA patients hospitalized from January 2016 to September 2019 were selected as observation group, with 55 at remission stage (observation group I) and 55 at acute attack stage (observation group II). Another 55 healthy volunteers were selected as control group. The correlations of IL-1β and COX-2 with uric acid (UA) were explored by Spearman’s analysis. IL-1β and COX-2 expression levels were compared at different time points after treatment.

Results: IL-1β, COX-2 and UA levels of patients were significantly higher in observation group I and II than those in the control group, and higher in the observation group II than those in observation group I. IL-1β and COX-2 were positively correlated with UA (P<0.05). ROC curve analysis showed that the optimal cut-off values of IL-1β and COX-2 for predicting GA were 18.23 and 9.12 pg/mL, and AUC values were 0.702 and 0.714 (P<0.001), respectively. On admission and after treatment for 3, 7, and 10 days, IL-1β and COX-2 levels of observation group II significantly exceeded those of observation group I (P<0.05). Compared with the efficacy group, after treatment for 14 days, IL-1β and COX-2 levels in serum and synovia significantly increased in the non-efficacy group (P<0.05).

Conclusion: IL-1β and COX-2 levels in serum and synovia of GA patients are evidently higher than those in the control group, which rise with the aggravation of disease.

Parole chiave

  • Interleukin-1β
  • Cyclooxygenase-2
  • Gouty arthritis
Accesso libero

An insight into the fecal microbiota composition in Romanian patients with ankylosing spondylitis using high-throughput 16S rRNA gene amplicon sequencing

Pubblicato online: 12 Feb 2022
Pagine: 49 - 61

Astratto

Abstract

Introduction. Application of next-generation sequencing technology generated a massive amount of information on the gut microbiome composition used to understand its role in the healthy state and in various diseases. We aimed to provide information on the gut microbiota composition of Romanian subjects diagnosed with ankylosing spondylitis, an immune-mediated arthropathy linked to a genetic predisposition and gut dysbiosis.

Methods. Stool samples collected from 25 patients with ankylosing spondylitis and 16 healthy controls were investigated using high-throughput DNA sequencing of 16S rRNA amplicons from seven different hypervariable regions and Ion Torrent PGM instrument. Microbial composition of metagenomic data was analyzed with QIIME software and differential abundance analysis of taxa encompassed linear discriminant analysis effect size (LEfSe).

Results. Overall, 14 phyla, 114 families, 114 genera, and 275 species were identified across the 41 samples, the aggregated data revealing as most abundant the phyla Bacteroidetes, Firmicutes, and Proteobacteria, the families Bacteroidaceae, Prevotellaceae, and Ruminococcaceae, the genera Bacteroides, Prevotella, and Faecalibacterium, and Prevotella copri species. Using various cutoffs for abundance and prevalence, core taxonomic members were identified which in general were shared between the patients and controls. However, evidence was gained that the diversity in the microbiomes from the former cohort was lower than for controls and that certain taxa had significantly different abundance between the two groups.

Conclusion. This study allowed an informative high-throughput 16S rRNA profiling of the gut microbiota needed to identify microbiome signatures of risk in the autochthonous population with AS.

Parole chiave

  • ankylosing spondylitis
  • next generation sequencing
  • gut microbiome
  • 16S rRNA amplicon sequencing
Accesso libero

The role of neutrophil-to-lymphocyte ratio as an outcome predictor factor in acute appendicitis: a single medical center’s experience

Pubblicato online: 12 Feb 2022
Pagine: 63 - 70

Astratto

Abstract

Introduction: Acute abdominal pain persists in defying modern diagnostic procedures. Inflammatory response variables in urine and blood tests can help rule out other disorders and support a clinical diagnosis of acute appendicitis (AA), but there is no specific blood test that can diagnose AA. In order to address this, our study sought to establish the value of (NLR) as a prognostic and severity predictor in patients with AA.

Material and method: We conducted an observational retrospective study of 356 patients who had undergone surgery for AA, assessing NLR as an outcome metric. The NLR was estimated using the differential WBC (white blood count) reported on admission; the data obtained were then recorded in a database and statistically analyzed.

Results: The NLR medians correlated (p<0.001) in uncomplicated AA, AA with localized peritonitis, and AA with generalized peritonitis (Kruskal-Wallis test). The ROC curve identified 81.4 % sensitivity, 62.5 % specificity, and a 73 % AUC in localized peritonitis. The NLR value was associated with generalized peritonitis with a sensitivity of 63.7 %, specificity of 64.3 %, and AUC of 68.2%. The NLR-patient death association had 100% sensitivity, 80.3% specificity, and 93.5 AUC.

Conclusion: NLR determination in individuals with acute appendicitis may be useful in predicting complications. NLR values greater than 7.86 are usually related with generalized peritonitis, while values larger than 12.9 may predict an increased risk of patient death. Calculating NLR from a complete blood count is a straightforward and cost-effective method of analyzing complicated AA in resource-constrained settings.

Parole chiave

  • appendicitis
  • inflammation
  • neutrophil-to-lymphocyte ratio
Accesso libero

Values of PCT and STREM-1 combined with clinical pulmonary infection score for the prognostic evaluation of elderly patients with ventilator-associated pneumonia

Pubblicato online: 12 Feb 2022
Pagine: 71 - 79

Astratto

Abstract

Objective: To explore the values of PCT and STREM-1 combined with clinical pulmonary infection score (CPIS) for evaluating ventilator-associated pneumonia (VAP) in elderly patients.

Methods: A total of 120 eligible patients were selected as VAP group, and 60 other patients without VAP were selected as control group. According to APACHE II scores, VAP group was divided into low-, medium-, and high-risk groups. The correlations of PCT, STREM-1, and CPIS with APACHE II score were analyzed. The values of PCT and STREM-1 combined with CPIS for evaluating prognosis were analyzed using ROC curves.

Results: In the VAP group, serum PCT and STREM-1 levels and CPIS after mechanical ventilation significantly exceeded those of the control group and before mechanical ventilation (P<0.05). These values were higher in the high-risk group than in medium- and low-risk groups (P<0.05). They were positively correlated with APACHE II score (P<0.001). These values of the death group were significantly higher than those of the survival group 1, 3, 5, and 7 d after treatment (P<0.05). AUC of PCT and STREM-1 combined with CPIS for assessing prognosis was 0.892 (P<0.001).

Conclusions: Serum PCT and STREM-1 levels and CPIS significantly increase in elderly patients with VAP, which rise with aggravation. Their combination has higher values for prognostic evaluation.

Parole chiave

  • procalcitonin
  • soluble triggering receptor expressed on myeloid cells-1
  • clinical pulmonary infection score
  • ventilator-associated pneumonia
Accesso libero

Expression of tight junction transmembrane protein Claudin-1 in gastric carcinoma and effects on tumor cell proliferation, invasion and migration

Pubblicato online: 12 Feb 2022
Pagine: 81 - 91

Astratto

Abstract

Background: Claudin-1 is involved in various cancers, but its expression and role in gastric carcinoma remain unclear.

Materials and Methods: Gastric carcinoma and adjacent normal tissues were harvested from 60 patients. Claudin-1 expression was detected by RT-qPCR. The expressions in human gastric carcinoma MKN45, SGC7901 and MKN28 cells and immortalized human gastric epithelium GES-1 cells were determined by RT-qPCR and Western blotting. Claudin-1 was overexpressed in SGC7901 cells by lentiviral transfection, and they were divided into Control (untransfected), normal control (NC) (transfected with lentiviral vector) and Claudin-1 (transfected with Claudin-1 overexpression lentivirus) groups. The proliferation, invasion and migration of gastric carcinoma cells were detected through cell counting kit-8, Transwell and wound healing assays, respectively. The effects of Claudin-1 on the expressions of epithelial-mesenchymal transition (EMT) marker proteins E-cadherin and N-cadherin were detected by Western blotting. Ten 4-week-old male BALB/c nude mice were subcutaneously injected with lentivirus-treated SGC7901 cells to establish the transplanted tumor model, and the effect of overexpression of Claudin-1 was explored.

Results: The expression of Claudin-1 in gastric carcinoma tissues was significantly lower than that in adjacent tissues (P<0.05). Overexpression of Claudin-1 significantly inhibited the proliferation, invasion and migration of SGC7901 cells, increased the expression of E-cadherin, and decreased that of N-cadherin (P<0.05). Overexpression of Claudin-1 in the mouse model significantly inhibited the growth of subcutaneous transplanted tumors (P<0.05).

Conclusion: Claudin-1 has low expression in gastric carcinoma tissues. Overexpression of Claudin-1 inhibits the proliferation, invasion, migration, and EMT of gastric carcinoma cells, and subcutaneous tumorigenesis in nude mice.

Parole chiave

  • proliferation
  • gastric cancer
  • invasion
  • Claudin-1
  • epithelial-mesenchymal transition
Accesso libero

Letter to the Editor

Pubblicato online: 12 Feb 2022
Pagine: 93 - 95

Astratto

Accesso libero

Evaluation of iron replacement therapy efficiency using a complex panel of biomarkers

Pubblicato online: 12 Feb 2022
Pagine: 97 - 105

Astratto

Accesso libero

Correction

Pubblicato online: 12 Feb 2022
Pagine: 107 - 107

Astratto

Accesso libero

Correction

Pubblicato online: 12 Feb 2022
Pagine: 108 - 108

Astratto

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