Rivista e Edizione

Volume 30 (2022): Edizione 3 (July 2022)

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Volume 29 (2021): Edizione 3 (July 2021)

Volume 29 (2021): Edizione 2 (April 2021)

Volume 29 (2021): Edizione 1 (January 2021)

Volume 28 (2020): Edizione 4 (October 2020)

Volume 28 (2020): Edizione 3 (July 2020)

Volume 28 (2020): Edizione 2 (April 2020)

Volume 28 (2020): Edizione 1 (January 2020)

Volume 27 (2019): Edizione 4 (October 2019)

Volume 27 (2019): Edizione 3 (July 2019)

Volume 27 (2019): Edizione 2 (April 2019)

Volume 27 (2019): Edizione 1 (January 2019)

Volume 26 (2018): Edizione 4 (October 2018)

Volume 26 (2018): Edizione 3 (July 2018)

Volume 26 (2018): Edizione 2 (April 2018)

Volume 26 (2018): Edizione 1 (January 2018)

Volume 25 (2017): Edizione 4 (October 2017)

Volume 25 (2017): Edizione 3 (July 2017)

Volume 25 (2017): Edizione 2 (April 2017)

Volume 25 (2017): Edizione 1 (January 2017)

Volume 24 (2016): Edizione 4 (December 2016)

Volume 24 (2016): Edizione 3 (September 2016)

Volume 24 (2016): Edizione 2 (June 2016)

Volume 24 (2016): Edizione 1 (March 2016)

Volume 23 (2015): Edizione 4 (December 2015)

Volume 23 (2015): Edizione 3 (August 2015)

Volume 23 (2015): Edizione 2 (June 2015)

Volume 23 (2015): Edizione 1 (March 2015)

Volume 22 (2014): Edizione 4 (December 2014)

Volume 22 (2014): Edizione 3 (September 2014)

Volume 22 (2014): Edizione 2 (June 2014)

Volume 22 (2014): Edizione 1 (March 2014)

Volume 21 (2013): Edizione 4 (December 2013)

Volume 21 (2013): Edizione 3 (September 2013)

Volume 21 (2013): Edizione 2 (June 2013)

Volume 21 (2013): Edizione 1 (March 2013)

Dettagli della rivista
Formato
Rivista
eISSN
2284-5623
Pubblicato per la prima volta
08 Aug 2013
Periodo di pubblicazione
4 volte all'anno
Lingue
Inglese

Cerca

Volume 25 (2017): Edizione 3 (July 2017)

Dettagli della rivista
Formato
Rivista
eISSN
2284-5623
Pubblicato per la prima volta
08 Aug 2013
Periodo di pubblicazione
4 volte all'anno
Lingue
Inglese

Cerca

8 Articoli

Review

Accesso libero

Involvement of inflammatory markers in pathogenesis of venous thromboembolism

Pubblicato online: 01 Aug 2017
Pagine: 227 - 236

Astratto

Abstract

Inflammation of the venous wall is involved in thrombogenesis, thrombus resolution, wall remodeling and the post-thrombotic syndrome. Different mechanisms are involved in both arterial and venous thrombosis and patients with atherothrombosis hold a higher risk of venous thrombosis. Although inflammation may represent the connection between arterial and venous thrombosis, it is not yet sure if it is the cause or consequence of venous thrombosis. Consequently, the relationships between inflammation markers as indicators of the inflammatory process and clinical venous thromboembolism need to be investigatd. For example, inflammation mediators such as the pro-inflammatory cytokines interleukin 8 (IL-8), IL-6, monocyte chemotactic protein 1 (MCP-1), C Reactive Protein (CRP), vascular cell adhesion molecule 1 (VCAM-1), intercellular adhesion molecule 1 (ICAM-1), matrix metalloproteinases and tumor necrosis factor alpha (TNF alpha) are all involved in thrombogenesis. Studies of venous thromboembolism on animal models proved that there are specific phases of the inflammatory process in venous thromboembolism and thrombus resolution. Knowing the molecular and immunologic mechanisms, identifying and understanding the inflammation markers which are relevant for venous thrombosis, can help to target specific pathways and to develop future therapies of this disease

Parole chiave

  • thrombembolism
  • markers
  • inflammation
  • thrombus
  • venous thrombosis

Original Research

Accesso libero

Association of ischemia-modified albumin with oxidative stress status and insulin resistance in obese patients

Pubblicato online: 01 Aug 2017
Pagine: 255 - 263

Astratto

Abstract

Objectives: Obesity is associated with oxidative stress due to the overproduction of free radicals in some accompanying states, such as hyperglycemia, elevated lipid levels and chronic inflammation. Free radical accumulation may modify the structure of human serum albumin, generating ischemia-modified albumin (IMA), and increased serum levels of IMA have been linked to obesity-related diseases and oxidative damage. The association of IMA levels with oxidative stress and insulin resistance (IR) has not been evaluated in the context of obesity. The aim of this study is to determine IMA levels in the context of obesity and their relationship with oxidative status and insulin resistance. Methods: Sixty-one adult obese cases with body mass index (BMI) ≥ 30 were evaluated, with 30 healthy adults with 18.5 ≤ BMI ≤ 24.9 included in the control group. IMA, total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), total cholesterol, triglycerides, HDL and LDL-cholesterols were determined. Results: IMA, TAS, TOS, OSI, total cholesterol and LDL-cholesterol levels were not different between the control and obese groups (P-value >0.05), while triglyceride levels were determined to be higher and HDL-cholesterol levels were determined to be lower in the obese group (P-value <0.05). When IMA, TAS, TOS, OSI levels were compared between the control/IR(-), obese/IR(+) and obese/IR(-) groups, no statistically significant differences were detected (P-value >0.05), but the fasting blood glucose level was determined to be higher in the obese/IR(+) group than in the control group. Conclusions: We concluded that obesity and insulin resistance had no effect on IMA levels in the obese group, who showed no impairment in their oxidative balance

Parole chiave

  • obesity
  • ischemia-modified albumin
  • oxidative stress
  • insulin resistance
Accesso libero

Stereological Evaluation of the Brains in Patients with Parkinson’s disease Compared to Controls

Pubblicato online: 01 Aug 2017
Pagine: 265 - 274

Astratto

Abstract

Parkinson’s disease (PD) is a chronic and progressive neurological disorder. A tetrad of bradykinesia, rigidity, tremor and postural instability are the core features of the disease. The aim of this study was to evaluate stereological changes in the brain of patients with PD and compare them with that of healthy controls. This case-control study was conducted on 29 patients with PD and 12 controls (C) in Zahedan, Iran. All subjects enrolled into the study through the convenience sampling method. MRI images of the brains of two groups in frontal and sagittal axis with consecutive 5mm distance slices were captured. Parameters including total volume (V) and volume density (Vv) of different parts of the brain were estimated based on Cavalries’ point counting stereological method. To analyze the data, descriptive statistics, Mann-Whitney U-Test applied for comparing the PD and C groups were used. Significance level was set at p<0.05. Our study showed that the volume of the brain and total volume and volume density (Vv) of cerebral hemispheres, cerebellum, ventricles, hippocampus, pons, mid brain and superior cerebellar peduncles in the PD group did not indicate significant difference from the control group. Total volume of brain stem in PD group wasn’t significantly different from the control group. The volume density of brain stem (p= 0.012) and total volume and volume density of middle cerebellar peduncle (p< 0.0001) in PD group were significantly larger than the control group. This study shows that PD stereological parameters related to volume and volume density of middle cerebellar peduncle and volume density of brain stem were significantly larger in patients compared to the controls. Therefore, stereological parameters can be used for early diagnosis and probably for follow-up in patients with PD.

Parole chiave

  • stereology
  • Parkinson’s disease
  • MRI
  • brain
  • quantitative analysis
Accesso libero

Laboratory diagnosis and follow-up of Romanian Gaucher disease patients

Pubblicato online: 01 Aug 2017
Pagine: 275 - 285

Astratto

Abstract

Background: Gaucher disease (GD) is caused by a recessively inherited deficiency of glucocerebrosidase which is encoded by the GBA gene in which nearly 450 mutations have been described. However, only a few genotype- phenotype correlations have been clearly established. The aim of this study was to investigate molecular features of GD in Romanian patients and to evaluate their impact on treatment response. Material and methods: 69 patients, diagnosed between 1997 and 2014 at our national referral laboratory, were included in this study. Frequent point mutations (N370S, L444P, 84GG, R463C) were detected by amplification and restriction enzyme digestion. Recombinant alleles (recTL, recNciI, recA456P) were screened by DNA sequencing. Plasma chitotriosidase served as a biomarker of disease severity throughout the follow-up period. Results: 66 patients had the non-neuronopathic (type 1) form of GD and 3 had the chronic neuronopathic (type 3) phenotype. We identified 79% of the mutant alleles, among which the most frequent mutations were N370S (54%) and L444P (18%). We found a statistically significant (p<0.001) and moderate to good correlation between the total therapeutic dose and the residual chitotriosidase activity (R = 0.621). After two years of treatment, we noticed statistically significant variations in chitotriosidase activity corresponding to the most frequent genotypes (N370S/ unknown allele, N370S/L444P, N370S/N370S and N370S/R463Q). Conclusions: Allele distribution displayed specific features in Romanian GD patients, such as the high prevalence of the N370S allele. Chitotriosidase activity measurement allowed the investigation of genotype influence on treatment outcome.

Parole chiave

  • Gaucher disease
  • genotype-phenotype correlations
  • chitotriosidase
  • enzyme replacement therapy
  • Romanian population
Accesso libero

Prediction of acute pancreatitis severity via the combined analysis of inflammatory biomarkers and coagulation parameters

Pubblicato online: 01 Aug 2017
Pagine: 237 - 244

Astratto

Abstract

Introduction. Timely assessment of severity of acute pancreatitis is needed to avoid severe systemic complications by making optimal therapeutic approach and correct prognosis of the disease. The aim of the study was to establish the role of several inflammatory biomarkers and coagulation parameters in prediction of AP severity, and also to propose a mathematical formula which allows their combined use for the same purpose. Material and Methods. The prospective study included 70 patients with AP. The patients were divided into groups: mild (group I), moderate (group II) and severe AP (group III). All patients were further classified into two groups: group A (mild AP) and group B (moderate and severe AP). Biochemical markers, inflammatory biomarkers and coagulation factors were tested in all patients. Results. Based on the results of Mann-Whitney,s test, it can be concluded that groups A and B are significant different from each other for CRP (p<0.05). Using the Wald’s stepwise forward method, a prediction model with CRP, PCT, D-dimer1, D-dimer3, fibrinogen1 and fibrinogen3 parameters as predictors of the severity of AP was obtained. The percentage of successful prediction of moderate or severe AP based on this model was 76.9%. The use of ROC analysis with the introduced linear combination from the logistic regression yielded equally good or even better results in the assessment of the severity of AP with the combined use of analyzed parameters. Conclusion. The combined analyses of biohumoral markers and coagulation parameters presented in the form a mathematical formula enabled a more accurate, rapid, rational and clinically available prediction of the severity of AP.

Parole chiave

  • acute pancreatitis
  • procalcitonin
  • C reactive protein
  • D dimer

Short Communication

Accesso libero

Investigation of biomarker variations post-return of spontaneous circulation following an out-of-hospital cardiac arrest

Pubblicato online: 01 Aug 2017
Pagine: 245 - 254

Astratto

Abstract

Objective: The objective of this research was to describe evolution of several biomarkers post-return of spontaneous circulation (ROSC) following an out-of-hospital cardiac arrest (OHCA). Methods: Thirteen adult patients were divided in 2 groups according to their survival status at 30 days, survivors (alive at 30 days or discharged alive) and non-survivors (not alive at 30 days). Glycemia, lactate, C-reactive protein (CRP), neurofilament heavy chain (NfH) and presepsin were assessed at pre-set time-points, during OHCA and the first 72 hours post-ROSC. Results: In survivors, lactate levels decreased steadily throughout the 72 hours from a maximum observed during OHCA; in non-survivors, it increased during ROSC, then decreased abruptly at 2 hours post-ROSC and remained lower than in survivors for up to 24 hours. Glycemia at all-time points within the first 24 hours and CRP levels at 2 hours post-ROSC were higher in non-survivors, but this observed difference was not statistically significant. The variation of NfH was bi-modal, with peaks at 12 and 48 hours. The interpretation of NfH was limited by the large number of samples outside the limit of detection. Conclusion: Glycemia, lactate and CRP showed different patterns of evolution in survivors and non-survivors and should be further investigated as potential predictors of survival after ROSC

Parole chiave

  • out of hospital cardiac arrest
  • return of spontaneous circulation
  • biomarkers

Case Report

Accesso libero

Genetic and Hormonal Determinations in a Pair of Identical Twins with Early Onset Psoriasis Vulgaris: Case Report and a Brief Review of the Literature

Pubblicato online: 01 Aug 2017
Pagine: 287 - 294

Astratto

Abstract

Psoriasis vulgaris is a chronic inflammatory dermatosis with major impact on patients’ life quality. The etiopathogenesis is multifactorial, depending on complex interactions between genetic and environmental factors. We present the case of two female patients, identical twins of 33 years old, suffering from psoriasis vulgaris since childhood. Patient A developed specific lesions of psoriasis at the age of 7 and patient B started to develop psoriasis lesions on the scalp two years later. At the age of 31, patient A was diagnosed with psoriatic arthritis. Laboratory test results were within the normal ranges for both patients. Hormonal and immunological determinations revealed the presence of a high level of antithyroidperoxidase antibody in patient A and increased level of prolactin in patient B. Ultrasonographic assessment of the thyroid detected the presence of bilateral micronodules in the first subject. Knowing that early onset psoriasis is associated with the presence of Human Leukocyte Antigen Cw6(HLA-Cw6), we aimed to confirm this hypothesis for our subjects. Although HLA-Cw6 is the most frequent mutation in psoriasis patients and it is present in about two-thirds of the tested subjects,the genetic results for both patients were negative, strengthening the fact that other factors, the environmental one and the hormonal disorders had an important role in their psoriasis pathogenesis. Under these conditions, we emphasize the importance of including a hormonal evaluation approach of psoriasis patients in order to diagnose and treat pathologies that may be related with disease exacerbations

Parole chiave

  • psoriasis
  • pathogenesis
  • genetic
  • hormones
  • twins
Accesso libero

Pitfalls in hemostasis exploration, a case report of a girl with Henoch-Schönlein type vasculitis

Pubblicato online: 01 Aug 2017
Pagine: 295 - 300

Astratto

Abstract

The adequate performance and correct interpretation of assays for coagulation factor inhibitors play a critical role for the hemostasis laboratory. Both, false positive and false negative inhibitor assays may be reported, leading to erroneous patient’s management. Therefore, we decided to present a case with a spurious image of an exceptionally rare acquired combined haemophilia A, B and C, with severe factor ( F) VIII, IX and XI deficiency, associated with high titre anti - F VIII, IX and XI inhibitors in a 4 years old girl with Henoch-Schönlein type vasculitis. Finally, performing, beside coagulometric methods also antigenic ELISA assays, we had to invalidate the diagnosis. The performance of antiphospholipd antibodies clarified the diagnosis , finally concluding as definite diagnosis Transient Lupus Anticoagulant Syndrome, with decisive impact on therapy and follow-up.

Parole chiave

  • acquired haemophilia
  • lupus anticoagulant
  • Henoch-Schönlein vasculitis
  • children
  • antiphospholipid antibody syndrome
8 Articoli

Review

Accesso libero

Involvement of inflammatory markers in pathogenesis of venous thromboembolism

Pubblicato online: 01 Aug 2017
Pagine: 227 - 236

Astratto

Abstract

Inflammation of the venous wall is involved in thrombogenesis, thrombus resolution, wall remodeling and the post-thrombotic syndrome. Different mechanisms are involved in both arterial and venous thrombosis and patients with atherothrombosis hold a higher risk of venous thrombosis. Although inflammation may represent the connection between arterial and venous thrombosis, it is not yet sure if it is the cause or consequence of venous thrombosis. Consequently, the relationships between inflammation markers as indicators of the inflammatory process and clinical venous thromboembolism need to be investigatd. For example, inflammation mediators such as the pro-inflammatory cytokines interleukin 8 (IL-8), IL-6, monocyte chemotactic protein 1 (MCP-1), C Reactive Protein (CRP), vascular cell adhesion molecule 1 (VCAM-1), intercellular adhesion molecule 1 (ICAM-1), matrix metalloproteinases and tumor necrosis factor alpha (TNF alpha) are all involved in thrombogenesis. Studies of venous thromboembolism on animal models proved that there are specific phases of the inflammatory process in venous thromboembolism and thrombus resolution. Knowing the molecular and immunologic mechanisms, identifying and understanding the inflammation markers which are relevant for venous thrombosis, can help to target specific pathways and to develop future therapies of this disease

Parole chiave

  • thrombembolism
  • markers
  • inflammation
  • thrombus
  • venous thrombosis

Original Research

Accesso libero

Association of ischemia-modified albumin with oxidative stress status and insulin resistance in obese patients

Pubblicato online: 01 Aug 2017
Pagine: 255 - 263

Astratto

Abstract

Objectives: Obesity is associated with oxidative stress due to the overproduction of free radicals in some accompanying states, such as hyperglycemia, elevated lipid levels and chronic inflammation. Free radical accumulation may modify the structure of human serum albumin, generating ischemia-modified albumin (IMA), and increased serum levels of IMA have been linked to obesity-related diseases and oxidative damage. The association of IMA levels with oxidative stress and insulin resistance (IR) has not been evaluated in the context of obesity. The aim of this study is to determine IMA levels in the context of obesity and their relationship with oxidative status and insulin resistance. Methods: Sixty-one adult obese cases with body mass index (BMI) ≥ 30 were evaluated, with 30 healthy adults with 18.5 ≤ BMI ≤ 24.9 included in the control group. IMA, total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), total cholesterol, triglycerides, HDL and LDL-cholesterols were determined. Results: IMA, TAS, TOS, OSI, total cholesterol and LDL-cholesterol levels were not different between the control and obese groups (P-value >0.05), while triglyceride levels were determined to be higher and HDL-cholesterol levels were determined to be lower in the obese group (P-value <0.05). When IMA, TAS, TOS, OSI levels were compared between the control/IR(-), obese/IR(+) and obese/IR(-) groups, no statistically significant differences were detected (P-value >0.05), but the fasting blood glucose level was determined to be higher in the obese/IR(+) group than in the control group. Conclusions: We concluded that obesity and insulin resistance had no effect on IMA levels in the obese group, who showed no impairment in their oxidative balance

Parole chiave

  • obesity
  • ischemia-modified albumin
  • oxidative stress
  • insulin resistance
Accesso libero

Stereological Evaluation of the Brains in Patients with Parkinson’s disease Compared to Controls

Pubblicato online: 01 Aug 2017
Pagine: 265 - 274

Astratto

Abstract

Parkinson’s disease (PD) is a chronic and progressive neurological disorder. A tetrad of bradykinesia, rigidity, tremor and postural instability are the core features of the disease. The aim of this study was to evaluate stereological changes in the brain of patients with PD and compare them with that of healthy controls. This case-control study was conducted on 29 patients with PD and 12 controls (C) in Zahedan, Iran. All subjects enrolled into the study through the convenience sampling method. MRI images of the brains of two groups in frontal and sagittal axis with consecutive 5mm distance slices were captured. Parameters including total volume (V) and volume density (Vv) of different parts of the brain were estimated based on Cavalries’ point counting stereological method. To analyze the data, descriptive statistics, Mann-Whitney U-Test applied for comparing the PD and C groups were used. Significance level was set at p<0.05. Our study showed that the volume of the brain and total volume and volume density (Vv) of cerebral hemispheres, cerebellum, ventricles, hippocampus, pons, mid brain and superior cerebellar peduncles in the PD group did not indicate significant difference from the control group. Total volume of brain stem in PD group wasn’t significantly different from the control group. The volume density of brain stem (p= 0.012) and total volume and volume density of middle cerebellar peduncle (p< 0.0001) in PD group were significantly larger than the control group. This study shows that PD stereological parameters related to volume and volume density of middle cerebellar peduncle and volume density of brain stem were significantly larger in patients compared to the controls. Therefore, stereological parameters can be used for early diagnosis and probably for follow-up in patients with PD.

Parole chiave

  • stereology
  • Parkinson’s disease
  • MRI
  • brain
  • quantitative analysis
Accesso libero

Laboratory diagnosis and follow-up of Romanian Gaucher disease patients

Pubblicato online: 01 Aug 2017
Pagine: 275 - 285

Astratto

Abstract

Background: Gaucher disease (GD) is caused by a recessively inherited deficiency of glucocerebrosidase which is encoded by the GBA gene in which nearly 450 mutations have been described. However, only a few genotype- phenotype correlations have been clearly established. The aim of this study was to investigate molecular features of GD in Romanian patients and to evaluate their impact on treatment response. Material and methods: 69 patients, diagnosed between 1997 and 2014 at our national referral laboratory, were included in this study. Frequent point mutations (N370S, L444P, 84GG, R463C) were detected by amplification and restriction enzyme digestion. Recombinant alleles (recTL, recNciI, recA456P) were screened by DNA sequencing. Plasma chitotriosidase served as a biomarker of disease severity throughout the follow-up period. Results: 66 patients had the non-neuronopathic (type 1) form of GD and 3 had the chronic neuronopathic (type 3) phenotype. We identified 79% of the mutant alleles, among which the most frequent mutations were N370S (54%) and L444P (18%). We found a statistically significant (p<0.001) and moderate to good correlation between the total therapeutic dose and the residual chitotriosidase activity (R = 0.621). After two years of treatment, we noticed statistically significant variations in chitotriosidase activity corresponding to the most frequent genotypes (N370S/ unknown allele, N370S/L444P, N370S/N370S and N370S/R463Q). Conclusions: Allele distribution displayed specific features in Romanian GD patients, such as the high prevalence of the N370S allele. Chitotriosidase activity measurement allowed the investigation of genotype influence on treatment outcome.

Parole chiave

  • Gaucher disease
  • genotype-phenotype correlations
  • chitotriosidase
  • enzyme replacement therapy
  • Romanian population
Accesso libero

Prediction of acute pancreatitis severity via the combined analysis of inflammatory biomarkers and coagulation parameters

Pubblicato online: 01 Aug 2017
Pagine: 237 - 244

Astratto

Abstract

Introduction. Timely assessment of severity of acute pancreatitis is needed to avoid severe systemic complications by making optimal therapeutic approach and correct prognosis of the disease. The aim of the study was to establish the role of several inflammatory biomarkers and coagulation parameters in prediction of AP severity, and also to propose a mathematical formula which allows their combined use for the same purpose. Material and Methods. The prospective study included 70 patients with AP. The patients were divided into groups: mild (group I), moderate (group II) and severe AP (group III). All patients were further classified into two groups: group A (mild AP) and group B (moderate and severe AP). Biochemical markers, inflammatory biomarkers and coagulation factors were tested in all patients. Results. Based on the results of Mann-Whitney,s test, it can be concluded that groups A and B are significant different from each other for CRP (p<0.05). Using the Wald’s stepwise forward method, a prediction model with CRP, PCT, D-dimer1, D-dimer3, fibrinogen1 and fibrinogen3 parameters as predictors of the severity of AP was obtained. The percentage of successful prediction of moderate or severe AP based on this model was 76.9%. The use of ROC analysis with the introduced linear combination from the logistic regression yielded equally good or even better results in the assessment of the severity of AP with the combined use of analyzed parameters. Conclusion. The combined analyses of biohumoral markers and coagulation parameters presented in the form a mathematical formula enabled a more accurate, rapid, rational and clinically available prediction of the severity of AP.

Parole chiave

  • acute pancreatitis
  • procalcitonin
  • C reactive protein
  • D dimer

Short Communication

Accesso libero

Investigation of biomarker variations post-return of spontaneous circulation following an out-of-hospital cardiac arrest

Pubblicato online: 01 Aug 2017
Pagine: 245 - 254

Astratto

Abstract

Objective: The objective of this research was to describe evolution of several biomarkers post-return of spontaneous circulation (ROSC) following an out-of-hospital cardiac arrest (OHCA). Methods: Thirteen adult patients were divided in 2 groups according to their survival status at 30 days, survivors (alive at 30 days or discharged alive) and non-survivors (not alive at 30 days). Glycemia, lactate, C-reactive protein (CRP), neurofilament heavy chain (NfH) and presepsin were assessed at pre-set time-points, during OHCA and the first 72 hours post-ROSC. Results: In survivors, lactate levels decreased steadily throughout the 72 hours from a maximum observed during OHCA; in non-survivors, it increased during ROSC, then decreased abruptly at 2 hours post-ROSC and remained lower than in survivors for up to 24 hours. Glycemia at all-time points within the first 24 hours and CRP levels at 2 hours post-ROSC were higher in non-survivors, but this observed difference was not statistically significant. The variation of NfH was bi-modal, with peaks at 12 and 48 hours. The interpretation of NfH was limited by the large number of samples outside the limit of detection. Conclusion: Glycemia, lactate and CRP showed different patterns of evolution in survivors and non-survivors and should be further investigated as potential predictors of survival after ROSC

Parole chiave

  • out of hospital cardiac arrest
  • return of spontaneous circulation
  • biomarkers

Case Report

Accesso libero

Genetic and Hormonal Determinations in a Pair of Identical Twins with Early Onset Psoriasis Vulgaris: Case Report and a Brief Review of the Literature

Pubblicato online: 01 Aug 2017
Pagine: 287 - 294

Astratto

Abstract

Psoriasis vulgaris is a chronic inflammatory dermatosis with major impact on patients’ life quality. The etiopathogenesis is multifactorial, depending on complex interactions between genetic and environmental factors. We present the case of two female patients, identical twins of 33 years old, suffering from psoriasis vulgaris since childhood. Patient A developed specific lesions of psoriasis at the age of 7 and patient B started to develop psoriasis lesions on the scalp two years later. At the age of 31, patient A was diagnosed with psoriatic arthritis. Laboratory test results were within the normal ranges for both patients. Hormonal and immunological determinations revealed the presence of a high level of antithyroidperoxidase antibody in patient A and increased level of prolactin in patient B. Ultrasonographic assessment of the thyroid detected the presence of bilateral micronodules in the first subject. Knowing that early onset psoriasis is associated with the presence of Human Leukocyte Antigen Cw6(HLA-Cw6), we aimed to confirm this hypothesis for our subjects. Although HLA-Cw6 is the most frequent mutation in psoriasis patients and it is present in about two-thirds of the tested subjects,the genetic results for both patients were negative, strengthening the fact that other factors, the environmental one and the hormonal disorders had an important role in their psoriasis pathogenesis. Under these conditions, we emphasize the importance of including a hormonal evaluation approach of psoriasis patients in order to diagnose and treat pathologies that may be related with disease exacerbations

Parole chiave

  • psoriasis
  • pathogenesis
  • genetic
  • hormones
  • twins
Accesso libero

Pitfalls in hemostasis exploration, a case report of a girl with Henoch-Schönlein type vasculitis

Pubblicato online: 01 Aug 2017
Pagine: 295 - 300

Astratto

Abstract

The adequate performance and correct interpretation of assays for coagulation factor inhibitors play a critical role for the hemostasis laboratory. Both, false positive and false negative inhibitor assays may be reported, leading to erroneous patient’s management. Therefore, we decided to present a case with a spurious image of an exceptionally rare acquired combined haemophilia A, B and C, with severe factor ( F) VIII, IX and XI deficiency, associated with high titre anti - F VIII, IX and XI inhibitors in a 4 years old girl with Henoch-Schönlein type vasculitis. Finally, performing, beside coagulometric methods also antigenic ELISA assays, we had to invalidate the diagnosis. The performance of antiphospholipd antibodies clarified the diagnosis , finally concluding as definite diagnosis Transient Lupus Anticoagulant Syndrome, with decisive impact on therapy and follow-up.

Parole chiave

  • acquired haemophilia
  • lupus anticoagulant
  • Henoch-Schönlein vasculitis
  • children
  • antiphospholipid antibody syndrome

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