<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The present study was aimed to obtain a close insight into the distribution and diversity of antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) among the aquatic products collected in Zhejiang, China. A total of 136 presumptive ARB picked up from six aquatic samples were classified into 22 genera and 49 species based on the 16S rDNA sequencing. <italic>Aeromonas</italic> spp., <italic>Shewanella</italic> spp., <italic>Acinetobacter</italic> spp., <italic>Myroides</italic> spp., <italic>Pseudomonas</italic> spp., and <italic>Citrobacter</italic> spp. accounted for 80% of the ARB. Among them, 109 isolates (80.15%) exhibited resistance to at least one antibiotic. Most isolates showed resistance to not only the originally selected drug but also to one to three other tested drugs. The diversity of ARB distributed in different aquatic products was significant. Furthermore, the resistance data obtained from genotypic tests were not entirely consistent with the results of the phenotypic evaluation. The genes <italic>qnr</italic>S, <italic>tet</italic>A, <italic>flo</italic>R, and <italic>cml</italic>A were frequently detected in their corresponding phenotypic resistant isolates. In contrast, the genes <italic>sul</italic>2, <italic>aac</italic>(6’)-Ib, and <italic>bla</italic><sub>PSE</sub> were less frequently found in the corresponding phenotypically resistant strains. The high diversity and detection rate of ARB and ARGs in aquaculture might be a significant threat to the food chains closely related to human health.<fig id="j_pjm-2023-010_ufig_001" position="float" fig-type="figure">
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</abstract>

The present study was aimed to obtain a close insight into the distribution and diversity of antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) among the aquatic products collected in Zhejiang, China. A total of 136 presumptive ARB picked up from six aquatic samples were classified into 22 genera and 49 species based on the 16S rDNA sequencing. Aeromonas spp., Shewanella spp., Acinetobacter spp., Myroides spp., Pseudomonas spp., and Citrobacter spp. accounted for 80% of the ARB. Among them, 109 isolates (80.15%) exhibited resistance to at least one antibiotic. Most isolates showed resistance to not only the originally selected drug but also to one to three other tested drugs. The diversity of ARB distributed in different aquatic products was significant. Furthermore, the resistance data obtained from genotypic tests were not entirely consistent with the results of the phenotypic evaluation. The genes qnrS, tetA, floR, and cmlA were frequently detected in their corresponding phenotypic resistant isolates. In contrast, the genes sul2, aac(6’)-Ib, and blaPSE were less frequently found in the corresponding phenotypically resistant strains. The high diversity and detection rate of ARB and ARGs in aquaculture might be a significant threat to the food chains closely related to human health.

Insight into the Antibiotic Resistance of Bacteria Isolated from Popular Aquatic Products Collected in Zhejiang, China

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The present study was aimed to obtain a close insight into the distribution and diversity of antibiotic-resistant bacteria (ARB) and antibiotic-resistance...

Insight into the Antibiotic Resistance of Bacteria Isolated from Popular Aquatic Products Collected in Zhejiang, China

Article Category: ORIGINAL PAPER

Published Online: Mar 24, 2023

Page range: 61 - 67

Received: Dec 02, 2022

Accepted: Feb 10, 2023

DOI: https://doi.org/10.33073/pjm-2023-010

Keywords
antibiotic resistance bacteria, antibiotic resistance gene, aquaculture, food safety

© 2023 JIAJIA WU et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Supplementary Material Details

Insight into the Antibiotic Resistance of Bacteria Isolated from Popular Aquatic Products Collected in Zhejiang, China

Article Category: ORIGINAL PAPER

Published Online: Mar 24, 2023

Page range: 61 - 67

Received: Dec 02, 2022

Accepted: Feb 10, 2023

DOI: https://doi.org/10.33073/pjm-2023-010

Keywordsantibiotic resistance bacteria, antibiotic resistance gene, aquaculture, food safety

© 2023 JIAJIA WU et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Supplementary Material Details

Keywords
antibiotic resistance bacteria, antibiotic resistance gene, aquaculture, food safety