Positive plasmid concentration | Intra-assay | Inter-assay | ||
---|---|---|---|---|
(copies/μL) | Ct (mean ± SD) | CV % | Ct (mean ± SD) | CV % |
2.20 × 105 | 18.15 ± 0.22 | 1.21 | 18.14 ± 0.25 | 1.38 |
2.20 × 104 | 21.48 ± 0.14 | 0.65 | 21.49 ± 0.18 | 0.84 |
2.20 × 103 | 24.98 ± 0.16 | 0.64 | 24.92 ± 0.28 | 1.12 |
2.20 × 102 | 28.56 ± 0.36 | 1.26 | 28.53 ± 0.48 | 1.68 |
Primer | Nucleotide sequence (5ʹ–3ʹ) | Nt position |
---|---|---|
PCV4-F | AATCTCACTGTCCACACCTG | 1105–1124 |
PCV4-R | CAAAACCCCAGGACCCATC | 1267–1249 |
PCV4-probe | FAM-ACCCACACCCTCCACTTCCAGC-BHQ1 |