<abstract xmlns="http://www.w3.org/1999/xhtml">

Cell envelope proteases (CEPs) can break down milk protein into peptides with different functions, which are of great benefit to human health. Therefore, the high-yield CEPs of <italic>Lactobacillus plantarum</italic> have the potential to produce functional dairy products. In previous experiments, we found that Na2HPO4, inulin, casein peptone and leucine have significant effects on CEP production by <italic>Lactobacillus plantarum</italic> LP69. So we proceeded to optimize the composition of the CEP-producing culture medium of <italic>L. plantarum</italic> through Box-Behnken design and response surface methodology. The protease activity, protein content and specific activity of CEPs produced by <italic>L. plantarum</italic> by inulin (0.2, 0.3, 0.4 %), casein peptone (0.4, 0.6, 0.8 %), Na2HPO4 (0.50, 0.52, 0.54 %) and leucine (14, 16, 18 mg/L) were evaluated. The optimal ratio of medium is 0.4 % inulin, 0.66 % casein peptone, 0.5 % Na2HPO4 and 14.04 mg/L Leucine. The final enzyme activity reached (24.46±0.81) U/mL, and the specific activity reached (1.41±0.46) U/mg.
</abstract>

Cell envelope proteases (CEPs) can break down milk protein into peptides with different functions, which are of great benefit to human health. Therefore, the high-yield CEPs of Lactobacillus plantarum have the potential to produce functional dairy products. In previous experiments, we found that Na2HPO4, inulin, casein peptone and leucine have significant effects on CEP production by Lactobacillus plantarum LP69. So we proceeded to optimize the composition of the CEP-producing culture medium of L. plantarum through Box-Behnken design and response surface methodology. The protease activity, protein content and specific activity of CEPs produced by L. plantarum by inulin (0.2, 0.3, 0.4 %), casein peptone (0.4, 0.6, 0.8 %), Na2HPO4 (0.50, 0.52, 0.54 %) and leucine (14, 16, 18 mg/L) were evaluated. The optimal ratio of medium is 0.4 % inulin, 0.66 % casein peptone, 0.5 % Na2HPO4 and 14.04 mg/L Leucine. The final enzyme activity reached (24.46±0.81) U/mL, and the specific activity reached (1.41±0.46) U/mg.

Effect of medium composition on cell envelope proteinase production by <italic>Lactobacillus plantarum</italic> LP69

Shaanxi University of Science & Technology

Acta Universitatis Cibiniensis. Series E: Food Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Effect of medium composition on cell envelope proteinase production by  LP69

Cell envelope proteases (CEPs) can break down milk protein into peptides with different functions, which are of great benefit to human health. Therefore, the...

Effect of medium composition on cell envelope proteinase production by Lactobacillus plantarum LP69

Published Online: Dec 30, 2021

Page range: 261 - 274

Received: Oct 02, 2021

Accepted: Dec 11, 2021

DOI: https://doi.org/10.2478/aucft-2021-0024

Keywords
LP69, cell-envelope proteinases, medium optimization, Box-Behnken design

© 2020 Chi Zhang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Effect of medium composition on cell envelope proteinase production by Lactobacillus plantarum LP69

Published Online: Dec 30, 2021

Page range: 261 - 274

Received: Oct 02, 2021

Accepted: Dec 11, 2021

DOI: https://doi.org/10.2478/aucft-2021-0024

KeywordsLP69, cell-envelope proteinases, medium optimization, Box-Behnken design

© 2020 Chi Zhang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
LP69, cell-envelope proteinases, medium optimization, Box-Behnken design