<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Cell-envelope proteinases (CEPs) can hydrolyze casein into functional peptides, which is beneficial to the health of the host. The single factor experiment screened out that the optimal conditions for CEP production by <italic>Lactobacillus plantarum LP69</italic> were 37°C, 20h, initial pH of 7, and optimal inoculation amount of 5%. The best conditions for this experiment were obtained by orthogonal experiment: time 22h, temperature 39°C, initial pH value of 6, and inoculation amount of 5%. Under this culture condition, the target protease activity of <italic>Lactobacillus plantarum</italic> LP69 reached 22.31±0.82U/mL, the protein content was 19.07±0.36mg/mL, and the specific activity was 1.17±0.06U/mg. The specific activity significantly increased by 15.8% compared with the control (<italic>p</italic>&lt;0.05).</p>
</abstract>

Cell-envelope proteinases (CEPs) can hydrolyze casein into functional peptides, which is beneficial to the health of the host. The single factor experiment screened out that the optimal conditions for CEP production by Lactobacillus plantarum LP69 were 37°C, 20h, initial pH of 7, and optimal inoculation amount of 5%. The best conditions for this experiment were obtained by orthogonal experiment: time 22h, temperature 39°C, initial pH value of 6, and inoculation amount of 5%. Under this culture condition, the target protease activity of Lactobacillus plantarum LP69 reached 22.31±0.82U/mL, the protein content was 19.07±0.36mg/mL, and the specific activity was 1.17±0.06U/mg. The specific activity significantly increased by 15.8% compared with the control (p<0.05).

Optimization of Fermentation Conditions for Cell Envelope Proteinase Produced by <italic>Lactobacillus Plantarum LP69</italic>

Shaanxi University of Science & Technology

Acta Universitatis Cibiniensis. Series E: Food Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Optimization of Fermentation Conditions for Cell Envelope Proteinase Produced by 

Cell-envelope proteinases (CEPs) can hydrolyze casein into functional peptides, which is beneficial to the health of the host. The single factor experiment...

Optimization of Fermentation Conditions for Cell Envelope Proteinase Produced by Lactobacillus Plantarum LP69

Published Online: Jun 28, 2021

Page range: 135 - 142

Received: Feb 25, 2021

Accepted: May 10, 2021

DOI: https://doi.org/10.2478/aucft-2021-0013

Keywords
cell-envelope proteinases, fermentation optimization, Orthogonal experiment

© 2021 Chi Zhang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Optimization of Fermentation Conditions for Cell Envelope Proteinase Produced by Lactobacillus Plantarum LP69

Published Online: Jun 28, 2021

Page range: 135 - 142

Received: Feb 25, 2021

Accepted: May 10, 2021

DOI: https://doi.org/10.2478/aucft-2021-0013

Keywordscell-envelope proteinases, fermentation optimization, Orthogonal experiment

© 2021 Chi Zhang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
cell-envelope proteinases, fermentation optimization, Orthogonal experiment