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qPCR analysis of mesenchymal stem cell marker expression during the long-term culture of canine adipocyte derived stem cells

Rut Bryl

Rut Bryl

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
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Bryl, Rut
, 
Claudia Dompe

Claudia Dompe

School of Medicine, Medical Sciences and Nutrition, University of AberdeenAberdeen, UK
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Dompe, Claudia
, 
Maurycy Jankowski

Maurycy Jankowski

Department of Anatomy, Poznań University of Medical SciencesPoznań, Poland
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Jankowski, Maurycy
, 
Katarzyna Stefańska

Katarzyna Stefańska

Department of Histology and Embryology, Poznań University of Medical SciencesPoznań, Poland
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Stefańska, Katarzyna
, 
Afsaneh Golkar Narenji

Afsaneh Golkar Narenji

Prestage Department of Poultry Science, North Carolina State UniversityRaleigh, USA
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Narenji, Afsaneh Golkar
, 
Jakub Kulus

Jakub Kulus

Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Kulus, Jakub
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Magdalena Kulus

Magdalena Kulus

Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Kulus, Magdalena
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Maria Wieczorkiewicz

Maria Wieczorkiewicz

Department of Basic and Preclinical Sciences, Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in ToruńToruń, Poland
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Wieczorkiewicz, Maria
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Grzegorz Wąsiatycz

Grzegorz Wąsiatycz

Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Wąsiatycz, Grzegorz
, 
Jędrzej M. Jaśkowski

Jędrzej M. Jaśkowski

Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Jaśkowski, Jędrzej M.
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Mariusz Kaczmarek

Mariusz Kaczmarek

Department of Cancer Immunology, Poznań University of Medical SciencesPoznań, Poland
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Kaczmarek, Mariusz
, 
James N. Petitte

James N. Petitte

Prestage Department of Poultry Science, North Carolina State UniversityRaleigh, USA
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Petitte, James N.
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Paul Mozdziak

Paul Mozdziak

  • Prestage Department of Poultry Science, North Carolina State UniversityRaleigh, USA
  • Physiology Graduate Program, North Carolina State UniversityRaleigh, USA
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Mozdziak, Paul
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Paweł Antosik

Paweł Antosik

Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Antosik, Paweł
 and 
Dorota Bukowska

Dorota Bukowska

Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in ToruńToruń, Poland
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Bukowska, Dorota
  
Dec 31, 2020
qPCR analysis of mesenchymal stem cell marker expression during the long-term culture of canine adipocyte derived stem cells's Cover Image
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Published Online: Dec 31, 2020
Page range: 139 - 145
Received: Oct 12, 2020
Accepted: Nov 15, 2020
DOI: https://doi.org/10.2478/acb-2020-0017
Keywords
© 2020 Rut Bryl, Claudia Dompe, Maurycy Jankowski, Katarzyna Stefańska, Afsaneh Golkar Narenji, Jakub Kulus, Magdalena Kulus, Maria Wieczorkiewicz, Grzegorz Wąsiatycz, Jędrzej M. Jaśkowski, Mariusz Kaczmarek, James N. Petitte, Paul Mozdziak, Paweł Antosik, Dorota Bukowska, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Photographs of morphological changes between the beginning and end-point of in vitro culture. Taken using an inverted microscope, using a relief contrast and 10x magnification.
Photographs of morphological changes between the beginning and end-point of in vitro culture. Taken using an inverted microscope, using a relief contrast and 10x magnification.

Figure 2

The results of the flow cytometry analysis of the available canine ASC markers. The coloured peaks represent the cells stained by the specific antibodies, while the transparent peaks correspond to isotype controls.
The results of the flow cytometry analysis of the available canine ASC markers. The coloured peaks represent the cells stained by the specific antibodies, while the transparent peaks correspond to isotype controls.

Figure 3

The results of the RT-qPCR analysis of the change in expression of ASC specific markers during a 14-day primary culture of adipocyte derived stem cells. All data was presented as a log10 of fold change.
The results of the RT-qPCR analysis of the change in expression of ASC specific markers during a 14-day primary culture of adipocyte derived stem cells. All data was presented as a log10 of fold change.

The sequences of primers used in the analysis

GENE NAMEFORWARD PRIMERREVERSE PRIMER
CD105CTCAGGTCCCCAATGCTACCGGTTGAAGGCCAGGTAGAGT
CD73CCCATTGACGAACGGAACAATATACCACGTGAATTCCGCC
CD14CACTAGAGCCCTGCGAAGTACGACGGCAATCATACACTGG
CD34ATGAGACCTCCAGCTGTGAGAGGTCAGACTGGTGCTTTCT
CD90CGAGAATGCTACCACCTTGCAGCCGGAGTTCACATGTGTA
CD45ACCTAGGCAAACATGTGAGGACTTCCAGATCAAAATTTCCACGA
HPRTCCATCACATCGTAGCCCTCACTTTTATATCGCCCGTTGAC
ACTBCCCTTGCCGCTCCGCCTTCGCAGCAATATCGGTCATCCAT
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Biochemistry
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