<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Three different fungal b-galactosidases, from three commercial sources, were studied for their capacity to produce galacto-oligosaccharides by trans-galactosylation from lactose. The enzymes were investigated in a soluble form for the GOS production at two concentrations of lactose (10 % and 30 % w/w). The maximum of GOS concentration (70.9 g · L<sup>-1</sup>) was obtained at 30 % of the initial lactose (pH 4.5, temperature 30 ℃) in the second hour of enzyme reaction. The GOS yield (24 % w/w) was not significantly affected by the process of immobilisation in comparison with the free enzyme preparation (22 % w/w), but time to obtain a maximal GOS concentration was prolonged. Galacto-oligosaccharides were effectively produced by immobilised β-galactosidase in the 15 repeated batch runs of 30 % (w/w) of lactose as the substrate.</p></abstract>

Three different fungal b-galactosidases, from three commercial sources, were studied for their capacity to produce galacto-oligosaccharides by trans-galactosylation from lactose. The enzymes were investigated in a soluble form for the GOS production at two concentrations of lactose (10 % and 30 % w/w). The maximum of GOS concentration (70.9 g · L-1) was obtained at 30 % of the initial lactose (pH 4.5, temperature 30 ℃) in the second hour of enzyme reaction. The GOS yield (24 % w/w) was not significantly affected by the process of immobilisation in comparison with the free enzyme preparation (22 % w/w), but time to obtain a maximal GOS concentration was prolonged. Galacto-oligosaccharides were effectively produced by immobilised β-galactosidase in the 15 repeated batch runs of 30 % (w/w) of lactose as the substrate.

Production of galacto-oligosaccharides by commercial preparates of fungal β-galactosidase

Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, 812 37 Bratislava, Slovak Republic

Acta Chimica Slovaca

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Three different fungal b-galactosidases, from three commercial sources, were studied for their capacity to produce galacto-oligosaccharides by...

Production of galacto-oligosaccharides by commercial preparates of fungal β-galactosidase

Published Online: Dec 05, 2015

Page range: 101 - 106

DOI: https://doi.org/10.1515/acs-2015-0018

Keywords
β-galactosidase, galacto-oligosaccharides, immobilisation

© Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Production of galacto-oligosaccharides by commercial preparates of fungal β-galactosidase

Published Online: Dec 05, 2015

Page range: 101 - 106

DOI: https://doi.org/10.1515/acs-2015-0018

Keywordsβ-galactosidase, galacto-oligosaccharides, immobilisation

© Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
β-galactosidase, galacto-oligosaccharides, immobilisation