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Special Zeszyt: This issue is dedicated to the memory of Milan Ryboš, DVM, D.Sc.

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Tom 47 (2010): Zeszyt 4 (December 2010)

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Tom 47 (2010): Zeszyt 1 (March 2010)

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Informacje o czasopiśmie
Format
Czasopismo
eISSN
1336-9083
ISSN
0440-6605
Pierwsze wydanie
22 Apr 2006
Częstotliwość wydawania
4 razy w roku
Języki
Angielski

Wyszukiwanie

Tom 47 (2010): Zeszyt 1 (March 2010)

Informacje o czasopiśmie
Format
Czasopismo
eISSN
1336-9083
ISSN
0440-6605
Pierwsze wydanie
22 Apr 2006
Częstotliwość wydawania
4 razy w roku
Języki
Angielski

Wyszukiwanie

10 Artykułów
Otwarty dostęp

An endemic area of Dirofilaria immitis seropositive dogs at the eastern foothills of Mt Olympus, Northern Greece

Data publikacji: 13 Feb 2010
Zakres stron: 3 - 7

Abstrakt

Abstract

Following an examination conducted on a population of three hundred and forty-one dogs living on the eastern foothills of Mt Olympus in Northern Greece, 17.9 % were found to be seropositive for the Dirofilaria immitis specific antigen. These results indicate that the region is an endemic area for the above-mentioned parasitosis in dogs. Serum samples were examined using the PetChek kit Snap (IDDEX Laboratories, ELISA, Portland USA) according to the manufacturer’s instructions.

With respect to gender, age and type of coat, the statistical analysis has shown that the prevalence of seropositive results is significantly higher (P < 0.05) among male dogs, older dogs and short-haired dogs. As regards breed and size, the same study has recorded a higher prevalence in mixed breed and small-sized dogs.

Otwarty dostęp

SSR data mined from expressed sequence tags of phytoparasitic nematodes

Data publikacji: 13 Feb 2010
Zakres stron: 8 - 19

Abstrakt

Abstract

SSR markers have become the most popular resource for studying population genetic variation in eukaryotes. However, few studies with SSR markers have been carried out with phytoparasitic nematodes. In this study a primary survey on EST-SSRs was made utilizing bioinformatics methods to derive SSRs from expressed sequence tags (ESTs) of 16 species of PPN, which belong to 7 genera and 5 families. The results showed that trinucleotide repeats were the most abundant SSRs in coding ESTs, while tetranucleotide SSRs were predominant in non-coding ESTs and genome sequences. AG/CT, AAC/GTT and (AAAN)n motifs were predominant, and CG/GC, ACT/AGT motifs were scanty in the ESTs of most genera and species. SSRs were more abundant in non-coding ESTs than in coding ESTs. The distribution frequencies of SSR motifs in coding ESTs, non-coding ESTs and genomes are different. Our results will provide useful information for screening SSRs from each genus and species for further genetic study of phytoparastic nematodes.

Słowa kluczowe

  • microsatellites
  • expressed sequence tags
  • plantparasitic nematodes
Otwarty dostęp

Prevalence of Paramphistomum infection in cattle and sheep in Van Province, Turkey

Data publikacji: 13 Feb 2010
Zakres stron: 20 - 24

Abstrakt

Abstract

This study was carried out in naturally infected cattle and sheep from May, 2006 to April 2007 in Van province, in the eastern of Turkey, to assess the prevalence of paramphistomosis. The rumen and reticulum of slaughtered cattle and sheep were collected monthly from abattoirs (Van Municipality of Slaughterhouse) to monitor the seasonal occurrence of paramphistomosis. 447 cattle and 948 sheep were examined, out of which 8.95 and 4.43 % were positive for Paramphistomum infection, with the mean intensity ± standard error of mean of 97.55 ± 12.50 and 89.17 ± 13.02, respectively. The highest infection in the cattle and sheep infected with Paramphistomum spp. was found during the autumn (September to November) (14.10 %, 8.33 %) and fallowed by the summer seasons (June to August) (9.02 %, 5.18 %).

Słowa kluczowe

  • Paramphistomosis
  • seasonal prevalence
  • cattle
  • sheep
  • pathology
  • Van
Otwarty dostęp

Infection of terrestial snails with larvae of Elaphostrongylus cervi (Nematoda, Protostrongylidae) in Białowieża National Park

Data publikacji: 13 Feb 2010
Zakres stron: 25 - 28

Abstrakt

Abstract

Species composition of intermediate hosts of Elaphostrongylus cervi — terrestrial snails, prevalence and intensity of their infection in natural invasions have been determined. The intermediate hosts of E. cervi are Succinea putris, Bradybena fruticum, Perforatella bidentata and Zonitoides nitidus. From June to August the percentage of snails S. putris infected with E. cervi larvae has been on constant level and in September and October decreased. Snails B. fruticum the highest prevalence of invasion showed on July and later it successively lowered. In the case of P. bidentata prevalence of E. cervi infection was the lowest in August, and than raised up to the highest value in October. For the first time the highest extensiveness of spontaneous infection has been found in P. bidentata. Till now as the most infected species of snail always has been noted S. putris. The highest intensity of infection — 242 larvae was found in B. fruticum, and little lower 73- 202 in S. putris. In two other species intensiveness ranged from 1 to 23 larvae. The most important role in spreading of E. cervi in Białowieża Forest fulfil S. putris and B. fruticum because of their high number in environment and P. bidens because of the highest intensiveness of infection.

Słowa kluczowe

  • Elaphostrongylus cervi
  • Cervides
  • Protostrongylidae
  • Nematodes
  • intermediate host
Otwarty dostęp

Paranaella luquei (Monogenea: Microcotylidae), an ectoparasite from the gills of Steindachnerina brevipinna, in the tributaries Corvo and Guairacá, Paranapanema River, Paraná, Brazil

Data publikacji: 13 Feb 2010
Zakres stron: 29 - 32

Abstrakt

Abstract

Paranaella luquei is a monogenean from family Microcotylidae, which was found parasitizing the gills of Steindachnerina brevipinna (Eigenmann & Eigenmann, 1889), collected in two different tributaries of Paranapanema River, Paraná, Brazil. This parasite was the first species of Microcotylidae described parasitizing freshwater fishes (Hypostomus sp., Hypostomus regani (Ihering) and Rhinelepis aspera Spix & Agassiz (Loricariidae) in the country.

Słowa kluczowe

  • Paranaella
  • Steindachnerina brevipinna
  • Microcotylidae
  • Corvo
  • Guairacá
Otwarty dostęp

A new cucullanid species (Nematoda) from the freshwater fish Thorichthys helleri (Cichlidae) in Mexico

Data publikacji: 13 Feb 2010
Zakres stron: 33 - 37

Abstrakt

Abstract

A new nematode species, Cucullanus oaxaquensis n. sp., is described from specimens recovered from the intestine of Thorichthys helleri Steindachner, 1864 from the Ró Modelo, Los Chimalapas, state of Oaxaca, Mexico. It is characterized largely by possesing unusual features among Cucullanus spp., i.e., net-like sculpture on the surface of eggs, a pair of small papillae near tail tip of females and a small terminal sclerotized structure at tail tip of both males and females. This findig represents the fouth species reported from freshwater fishes in Mexico and the second for cichlid fishes.

Słowa kluczowe

  • Nematoda
  • Cucullanus
  • Cichlidae
  • Thorichthys helleri
  • Oaxaca
  • Mexico
Otwarty dostęp

A new species of Centrorhynchus (Acanthocephala, Centrorhynchidae) endoparasite of Guira guira (Aves, Cuculidae) from Argentina

Data publikacji: 13 Feb 2010
Zakres stron: 38 - 47

Abstrakt

Abstract

Centrorhynchus guira n. sp. is described from the guira cuckoo, Guira guira (Gmelin) (Cuculiformes: Cuculidae), from Argentina. This new species is characterized by the proboscis armament with 32 longitudinal rows (29 with 18 hooks per row and 3 with 19 hooks per row) and by the following hook pattern per row: 8–9 true hooks, 4 transitional hooks with lateral alate processes, and 6 spiniform hooks. The presence of transitional hooks is shared with five other Neotropical species in the genus: C. polymorphus, C. albidus, C. microcephalus, C. kuntzi and C. crotophagicola. Among these species, C. guira n. sp. most closely resembles C. kuntzi in having transitional hooks with lateral alate processes, however differs in the number of hooks per row and spiniform hooks. This is the first record of the genus Centrorhynchus parasitizing Argentinean birds. A key to the species of the Neotropical Centrorhynchus Lühe 1911 is presented.

Słowa kluczowe

  • Centrorhynchidae
  • Centrorhynchus guira n. sp.
  • Guira guira
  • Cuculidae
  • Argentina
Otwarty dostęp

Effect of chestnut tannins on the root-knot nematode Meloidogyne javanica

Data publikacji: 13 Feb 2010
Zakres stron: 48 - 57

Abstrakt

Abstract

Among the natural products extracted from plants, tannins have been reported to possess antihelmintic properties especially for gastrointestinal nematodes in ruminants. Also, they are toxic to a wide range of fungi, bacteria and yeasts. Therefore, an in vitro and a glasshouse pot experiments were undertaken to evaluate the effect of chestnut tannins on the control of the root-knot nematode Meloidogyne javanica. In the in vitro experiment, different tannin concentrations in a geometric scale (from 0.32 to 20.48 g/l), were tested for their effect on hatching of the nematode, whereas in the pot experiment, 100, 250 and 450 g/m2 of tannins in aqueous solutions, were used in pots at transplant or at transplant and two weeks later for their effect on nematode control. In both experiments treatments were compared to untreated and fenamiphos-treated controls. In vitro a nematostatic effect of tannins was observed, whereas in the pot experiment a significant reduction of eggs and juveniles/g root, total population density and reproduction rates of the nematode were recorded. The anatomical changes induced by M. javanica in tomato roots treated with tannins did not differ from those produced by this and other Meloidogyne species on various hosts reported earlier.

Słowa kluczowe

  • Meloidogyne javanica
  • nematode control
  • chestnut tannins
Otwarty dostęp

Abundance and diversity of soil nematodes as influenced by different types of organic manure

Data publikacji: 13 Feb 2010
Zakres stron: 58 - 66

Abstrakt

Abstract

Nematode abundance and diversity from different types of organic manure soil treatments were investigated in a longterm field experiment carried out in Qu-Zhou experimental station, China Agricultural University. The composts used in the experiment were a traditional compost (C) (60 % straw, 30 % livestock dung, 5 % cottonseed-pressed trash and 5 % brans), traditional compost and chicken dung compost (60 % straw, 30 % chiken dung, 5 % cottonseedpressed trash and 5 % brans) added with effective microorganisms, EMC and EMCDC respectively. Six treatments were arranged according to a randomized block design with three replicates per treatment. Treatments were incorporation into the soil of compost EMC, EMCDC, and C each at the rates of 7.5 and 15 t/ha. Plots were sown with winter wheat (Triticum aestivum L.) and summer maize (Zea mays L.) every year from 1997 to 2004. Overall, 28 nematode genera were found. Seven genera were bacterivores, 3 genera were fungivores, 13 genera were plantparasites and 5 genera were omnivores-predators. The Rhabditis, Cephalobus, Helicotylenchus were dominant genera in the present study. The bacterivores and plantparasitic nematodes were the dominant trophic groups except in C treatment. Nematode abundance per 100 g dried soil ranged from 372 to 553. Addition of effective microorganism increased the number and proportion of bacterivorous nematodes and decreased the number and proportion of plant-parasitic nematodes compared to traditional compost C. Total number of nematode was significantly influenced by compost amount, but didn’t significantly influence by EM agent. Total abundance of nematode was positively correlated with the content of soil organic matter, total N, available P and K. The long-term addition of EM agent hasn’t adverse effect on soil nematode community.

Słowa kluczowe

  • effective microorganism
  • organic manure
  • soil nematodes
  • biodiversity
Otwarty dostęp

First record of Paratrophurus hungaricus Andrássy, 1973 (Tylenchida, Telotylenchidae) from Slovakia

Data publikacji: 13 Feb 2010
Zakres stron: 67 - 68

Abstrakt

Abstract

In soil of floodplain forests along the Danube and Moravia Rivers a rare nematode species Paratrophurus hungaricus Andrássy, 1973 was recorded for the first time from the territory of Slovakia. Presented here are some notes on morphology and ecology of the species observed.

Słowa kluczowe

  • Paratrophurus hungaricus
  • morphology
  • ecology
  • floodplain forest
  • Slovakia
10 Artykułów
Otwarty dostęp

An endemic area of Dirofilaria immitis seropositive dogs at the eastern foothills of Mt Olympus, Northern Greece

Data publikacji: 13 Feb 2010
Zakres stron: 3 - 7

Abstrakt

Abstract

Following an examination conducted on a population of three hundred and forty-one dogs living on the eastern foothills of Mt Olympus in Northern Greece, 17.9 % were found to be seropositive for the Dirofilaria immitis specific antigen. These results indicate that the region is an endemic area for the above-mentioned parasitosis in dogs. Serum samples were examined using the PetChek kit Snap (IDDEX Laboratories, ELISA, Portland USA) according to the manufacturer’s instructions.

With respect to gender, age and type of coat, the statistical analysis has shown that the prevalence of seropositive results is significantly higher (P < 0.05) among male dogs, older dogs and short-haired dogs. As regards breed and size, the same study has recorded a higher prevalence in mixed breed and small-sized dogs.

Otwarty dostęp

SSR data mined from expressed sequence tags of phytoparasitic nematodes

Data publikacji: 13 Feb 2010
Zakres stron: 8 - 19

Abstrakt

Abstract

SSR markers have become the most popular resource for studying population genetic variation in eukaryotes. However, few studies with SSR markers have been carried out with phytoparasitic nematodes. In this study a primary survey on EST-SSRs was made utilizing bioinformatics methods to derive SSRs from expressed sequence tags (ESTs) of 16 species of PPN, which belong to 7 genera and 5 families. The results showed that trinucleotide repeats were the most abundant SSRs in coding ESTs, while tetranucleotide SSRs were predominant in non-coding ESTs and genome sequences. AG/CT, AAC/GTT and (AAAN)n motifs were predominant, and CG/GC, ACT/AGT motifs were scanty in the ESTs of most genera and species. SSRs were more abundant in non-coding ESTs than in coding ESTs. The distribution frequencies of SSR motifs in coding ESTs, non-coding ESTs and genomes are different. Our results will provide useful information for screening SSRs from each genus and species for further genetic study of phytoparastic nematodes.

Słowa kluczowe

  • microsatellites
  • expressed sequence tags
  • plantparasitic nematodes
Otwarty dostęp

Prevalence of Paramphistomum infection in cattle and sheep in Van Province, Turkey

Data publikacji: 13 Feb 2010
Zakres stron: 20 - 24

Abstrakt

Abstract

This study was carried out in naturally infected cattle and sheep from May, 2006 to April 2007 in Van province, in the eastern of Turkey, to assess the prevalence of paramphistomosis. The rumen and reticulum of slaughtered cattle and sheep were collected monthly from abattoirs (Van Municipality of Slaughterhouse) to monitor the seasonal occurrence of paramphistomosis. 447 cattle and 948 sheep were examined, out of which 8.95 and 4.43 % were positive for Paramphistomum infection, with the mean intensity ± standard error of mean of 97.55 ± 12.50 and 89.17 ± 13.02, respectively. The highest infection in the cattle and sheep infected with Paramphistomum spp. was found during the autumn (September to November) (14.10 %, 8.33 %) and fallowed by the summer seasons (June to August) (9.02 %, 5.18 %).

Słowa kluczowe

  • Paramphistomosis
  • seasonal prevalence
  • cattle
  • sheep
  • pathology
  • Van
Otwarty dostęp

Infection of terrestial snails with larvae of Elaphostrongylus cervi (Nematoda, Protostrongylidae) in Białowieża National Park

Data publikacji: 13 Feb 2010
Zakres stron: 25 - 28

Abstrakt

Abstract

Species composition of intermediate hosts of Elaphostrongylus cervi — terrestrial snails, prevalence and intensity of their infection in natural invasions have been determined. The intermediate hosts of E. cervi are Succinea putris, Bradybena fruticum, Perforatella bidentata and Zonitoides nitidus. From June to August the percentage of snails S. putris infected with E. cervi larvae has been on constant level and in September and October decreased. Snails B. fruticum the highest prevalence of invasion showed on July and later it successively lowered. In the case of P. bidentata prevalence of E. cervi infection was the lowest in August, and than raised up to the highest value in October. For the first time the highest extensiveness of spontaneous infection has been found in P. bidentata. Till now as the most infected species of snail always has been noted S. putris. The highest intensity of infection — 242 larvae was found in B. fruticum, and little lower 73- 202 in S. putris. In two other species intensiveness ranged from 1 to 23 larvae. The most important role in spreading of E. cervi in Białowieża Forest fulfil S. putris and B. fruticum because of their high number in environment and P. bidens because of the highest intensiveness of infection.

Słowa kluczowe

  • Elaphostrongylus cervi
  • Cervides
  • Protostrongylidae
  • Nematodes
  • intermediate host
Otwarty dostęp

Paranaella luquei (Monogenea: Microcotylidae), an ectoparasite from the gills of Steindachnerina brevipinna, in the tributaries Corvo and Guairacá, Paranapanema River, Paraná, Brazil

Data publikacji: 13 Feb 2010
Zakres stron: 29 - 32

Abstrakt

Abstract

Paranaella luquei is a monogenean from family Microcotylidae, which was found parasitizing the gills of Steindachnerina brevipinna (Eigenmann & Eigenmann, 1889), collected in two different tributaries of Paranapanema River, Paraná, Brazil. This parasite was the first species of Microcotylidae described parasitizing freshwater fishes (Hypostomus sp., Hypostomus regani (Ihering) and Rhinelepis aspera Spix & Agassiz (Loricariidae) in the country.

Słowa kluczowe

  • Paranaella
  • Steindachnerina brevipinna
  • Microcotylidae
  • Corvo
  • Guairacá
Otwarty dostęp

A new cucullanid species (Nematoda) from the freshwater fish Thorichthys helleri (Cichlidae) in Mexico

Data publikacji: 13 Feb 2010
Zakres stron: 33 - 37

Abstrakt

Abstract

A new nematode species, Cucullanus oaxaquensis n. sp., is described from specimens recovered from the intestine of Thorichthys helleri Steindachner, 1864 from the Ró Modelo, Los Chimalapas, state of Oaxaca, Mexico. It is characterized largely by possesing unusual features among Cucullanus spp., i.e., net-like sculpture on the surface of eggs, a pair of small papillae near tail tip of females and a small terminal sclerotized structure at tail tip of both males and females. This findig represents the fouth species reported from freshwater fishes in Mexico and the second for cichlid fishes.

Słowa kluczowe

  • Nematoda
  • Cucullanus
  • Cichlidae
  • Thorichthys helleri
  • Oaxaca
  • Mexico
Otwarty dostęp

A new species of Centrorhynchus (Acanthocephala, Centrorhynchidae) endoparasite of Guira guira (Aves, Cuculidae) from Argentina

Data publikacji: 13 Feb 2010
Zakres stron: 38 - 47

Abstrakt

Abstract

Centrorhynchus guira n. sp. is described from the guira cuckoo, Guira guira (Gmelin) (Cuculiformes: Cuculidae), from Argentina. This new species is characterized by the proboscis armament with 32 longitudinal rows (29 with 18 hooks per row and 3 with 19 hooks per row) and by the following hook pattern per row: 8–9 true hooks, 4 transitional hooks with lateral alate processes, and 6 spiniform hooks. The presence of transitional hooks is shared with five other Neotropical species in the genus: C. polymorphus, C. albidus, C. microcephalus, C. kuntzi and C. crotophagicola. Among these species, C. guira n. sp. most closely resembles C. kuntzi in having transitional hooks with lateral alate processes, however differs in the number of hooks per row and spiniform hooks. This is the first record of the genus Centrorhynchus parasitizing Argentinean birds. A key to the species of the Neotropical Centrorhynchus Lühe 1911 is presented.

Słowa kluczowe

  • Centrorhynchidae
  • Centrorhynchus guira n. sp.
  • Guira guira
  • Cuculidae
  • Argentina
Otwarty dostęp

Effect of chestnut tannins on the root-knot nematode Meloidogyne javanica

Data publikacji: 13 Feb 2010
Zakres stron: 48 - 57

Abstrakt

Abstract

Among the natural products extracted from plants, tannins have been reported to possess antihelmintic properties especially for gastrointestinal nematodes in ruminants. Also, they are toxic to a wide range of fungi, bacteria and yeasts. Therefore, an in vitro and a glasshouse pot experiments were undertaken to evaluate the effect of chestnut tannins on the control of the root-knot nematode Meloidogyne javanica. In the in vitro experiment, different tannin concentrations in a geometric scale (from 0.32 to 20.48 g/l), were tested for their effect on hatching of the nematode, whereas in the pot experiment, 100, 250 and 450 g/m2 of tannins in aqueous solutions, were used in pots at transplant or at transplant and two weeks later for their effect on nematode control. In both experiments treatments were compared to untreated and fenamiphos-treated controls. In vitro a nematostatic effect of tannins was observed, whereas in the pot experiment a significant reduction of eggs and juveniles/g root, total population density and reproduction rates of the nematode were recorded. The anatomical changes induced by M. javanica in tomato roots treated with tannins did not differ from those produced by this and other Meloidogyne species on various hosts reported earlier.

Słowa kluczowe

  • Meloidogyne javanica
  • nematode control
  • chestnut tannins
Otwarty dostęp

Abundance and diversity of soil nematodes as influenced by different types of organic manure

Data publikacji: 13 Feb 2010
Zakres stron: 58 - 66

Abstrakt

Abstract

Nematode abundance and diversity from different types of organic manure soil treatments were investigated in a longterm field experiment carried out in Qu-Zhou experimental station, China Agricultural University. The composts used in the experiment were a traditional compost (C) (60 % straw, 30 % livestock dung, 5 % cottonseed-pressed trash and 5 % brans), traditional compost and chicken dung compost (60 % straw, 30 % chiken dung, 5 % cottonseedpressed trash and 5 % brans) added with effective microorganisms, EMC and EMCDC respectively. Six treatments were arranged according to a randomized block design with three replicates per treatment. Treatments were incorporation into the soil of compost EMC, EMCDC, and C each at the rates of 7.5 and 15 t/ha. Plots were sown with winter wheat (Triticum aestivum L.) and summer maize (Zea mays L.) every year from 1997 to 2004. Overall, 28 nematode genera were found. Seven genera were bacterivores, 3 genera were fungivores, 13 genera were plantparasites and 5 genera were omnivores-predators. The Rhabditis, Cephalobus, Helicotylenchus were dominant genera in the present study. The bacterivores and plantparasitic nematodes were the dominant trophic groups except in C treatment. Nematode abundance per 100 g dried soil ranged from 372 to 553. Addition of effective microorganism increased the number and proportion of bacterivorous nematodes and decreased the number and proportion of plant-parasitic nematodes compared to traditional compost C. Total number of nematode was significantly influenced by compost amount, but didn’t significantly influence by EM agent. Total abundance of nematode was positively correlated with the content of soil organic matter, total N, available P and K. The long-term addition of EM agent hasn’t adverse effect on soil nematode community.

Słowa kluczowe

  • effective microorganism
  • organic manure
  • soil nematodes
  • biodiversity
Otwarty dostęp

First record of Paratrophurus hungaricus Andrássy, 1973 (Tylenchida, Telotylenchidae) from Slovakia

Data publikacji: 13 Feb 2010
Zakres stron: 67 - 68

Abstrakt

Abstract

In soil of floodplain forests along the Danube and Moravia Rivers a rare nematode species Paratrophurus hungaricus Andrássy, 1973 was recorded for the first time from the territory of Slovakia. Presented here are some notes on morphology and ecology of the species observed.

Słowa kluczowe

  • Paratrophurus hungaricus
  • morphology
  • ecology
  • floodplain forest
  • Slovakia

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