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<p>The present study was aimed to obtain a close insight into the distribution and diversity of antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) among the aquatic products collected in Zhejiang, China. A total of 136 presumptive ARB picked up from six aquatic samples were classified into 22 genera and 49 species based on the 16S rDNA sequencing. <italic>Aeromonas</italic> spp., <italic>Shewanella</italic> spp., <italic>Acinetobacter</italic> spp., <italic>Myroides</italic> spp., <italic>Pseudomonas</italic> spp., and <italic>Citrobacter</italic> spp. accounted for 80% of the ARB. Among them, 109 isolates (80.15%) exhibited resistance to at least one antibiotic. Most isolates showed resistance to not only the originally selected drug but also to one to three other tested drugs. The diversity of ARB distributed in different aquatic products was significant. Furthermore, the resistance data obtained from genotypic tests were not entirely consistent with the results of the phenotypic evaluation. The genes <italic>qnr</italic>S, <italic>tet</italic>A, <italic>flo</italic>R, and <italic>cml</italic>A were frequently detected in their corresponding phenotypic resistant isolates. In contrast, the genes <italic>sul</italic>2, <italic>aac</italic>(6’)-Ib, and <italic>bla</italic><sub>PSE</sub> were less frequently found in the corresponding phenotypically resistant strains. The high diversity and detection rate of ARB and ARGs in aquaculture might be a significant threat to the food chains closely related to human health.<fig id="j_pjm-2023-010_ufig_001" position="float" fig-type="figure">
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</abstract>

The present study was aimed to obtain a close insight into the distribution and diversity of antibiotic-resistant bacteria (ARB) and antibiotic-resistance genes (ARGs) among the aquatic products collected in Zhejiang, China. A total of 136 presumptive ARB picked up from six aquatic samples were classified into 22 genera and 49 species based on the 16S rDNA sequencing. Aeromonas spp., Shewanella spp., Acinetobacter spp., Myroides spp., Pseudomonas spp., and Citrobacter spp. accounted for 80% of the ARB. Among them, 109 isolates (80.15%) exhibited resistance to at least one antibiotic. Most isolates showed resistance to not only the originally selected drug but also to one to three other tested drugs. The diversity of ARB distributed in different aquatic products was significant. Furthermore, the resistance data obtained from genotypic tests were not entirely consistent with the results of the phenotypic evaluation. The genes qnrS, tetA, floR, and cmlA were frequently detected in their corresponding phenotypic resistant isolates. In contrast, the genes sul2, aac(6’)-Ib, and blaPSE were less frequently found in the corresponding phenotypically resistant strains. The high diversity and detection rate of ARB and ARGs in aquaculture might be a significant threat to the food chains closely related to human health.

Insight into the Antibiotic Resistance of Bacteria Isolated from Popular Aquatic Products Collected in Zhejiang, China

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The present study was aimed to obtain a close insight into the distribution and diversity of antibiotic-resistant bacteria (ARB) and antibiotic-resistance...

Antimicrobial	No. of ARB isolates	ARGs	No. of the ARGs carrier	Coincidence rate (%)
Sulfadiazine	50	sul2	10	20%
Gentamicin	24	aac(6’)-Ib	10	42%
Enrofloxacin	35	qnrS	26	74%
Cephalexin	37	bla_PSE	12	32%
Tetracycline	52	tetA	35	67%
Chloramphenicol	38	floR	30	79%
Chloramphenicol	38	cmlA	30	79%

Insight into the Antibiotic Resistance of Bacteria Isolated from Popular Aquatic Products Collected in Zhejiang, China

Article Category: ORIGINAL PAPER

Data publikacji: 24 mar 2023

Zakres stron: 61 - 67

Otrzymano: 02 gru 2022

Przyjęty: 10 lut 2023

DOI: https://doi.org/10.33073/pjm-2023-010

Słowa kluczowe
antibiotic resistance bacteria, antibiotic resistance gene, aquaculture, food safety

© 2023 JIAJIA WU et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

The coincidence of the resistant phenotype and genotype among the isolates.

Insight into the Antibiotic Resistance of Bacteria Isolated from Popular Aquatic Products Collected in Zhejiang, China

Article Category: ORIGINAL PAPER

Data publikacji: 24 mar 2023

Zakres stron: 61 - 67

Otrzymano: 02 gru 2022

Przyjęty: 10 lut 2023

DOI: https://doi.org/10.33073/pjm-2023-010

Słowa kluczoweantibiotic resistance bacteria, antibiotic resistance gene, aquaculture, food safety

© 2023 JIAJIA WU et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

The coincidence of the resistant phenotype and genotype among the isolates.

Słowa kluczowe
antibiotic resistance bacteria, antibiotic resistance gene, aquaculture, food safety