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In vitro and in vivo activity of Lactobacillus sakei L14 strain against Campylobacter jejuni DC3 strain

John Roybert P. Catacutan

John Roybert P. Catacutan

Institute of Biology, National Science Complex, College of Science University of the Philippines DilimanQuezon City, Philippines
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Catacutan, John Roybert P.
, 
Ma. Socorro Edden P. Subejano

Ma. Socorro Edden P. Subejano

Institute of Biology, National Science Complex, College of Science University of the Philippines DilimanQuezon City, Philippines
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Subejano, Ma. Socorro Edden P.
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Gil M. Penuliar

Gil M. Penuliar

Institute of Biology, National Science Complex, College of Science University of the Philippines DilimanQuezon City, Philippines
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Penuliar, Gil M.
  
25 mar 2022
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Data publikacji: 25 mar 2022
Zakres stron: 85 - 94
Otrzymano: 12 wrz 2021
Przyjęty: 06 mar 2022
DOI: https://doi.org/10.2478/jvetres-2022-0015
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© 2022 J.R.P. Catacutan et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Domestic poultry is a natural reservoir of Campylobacter, the host–pathogen interaction being predominantly asymptomatic. This study investigated whether chickens remain asymptomatic partly because of lactic acid bacteria (LAB).

Material and Methods

Campylobacter spp. and LAB were isolated from the gut of poultry chickens using enrichment and screening assays and were identified via rDNA sequencing. The C. jejuni DC3 isolate was grown in different cell-free supernatants (CFS) generated from a priority LAB isolate. An in vivo challenge involving the C. jejuni and LAB isolates using a chicken model was performed to confirm the in vitro findings.

Results

Twelve presumptive LAB isolates had anti-C. jejuni activity based on cross-streak and agar plug assays, with Lactobacillus sakei L14 isolate exhibiting the highest activity. Inhibition by L. sakei L14 CFS of the growth of C. jejuni occurred in a dose-dependent manner. Campylobacter jejuni DC3 inhibition was most evident in CFS harvested at 72 h and produced by co-culture with the pathogen. Neutralisation of the CFS abrogated the observed inhibition. Co-infection with C. jejuni DC3 and L. sakei L14 in vivo, however, failed to inhibit C. jejuni colonisation in chickens.

Conclusion

The results suggest that the anti-C. jejuni effect of L. sakei L14 in chickens may be due to mechanisms other than direct inhibition of growth.
Język:
Angielski
Częstotliwość wydawania:
4 razy w roku
Dziedziny czasopisma:
Nauki biologiczne, Biologia molekularna, Mikrobiologia i wirusologia, Nauki biologiczne, inne, Medycyna, Weterynaria
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