Accès libre

In vitro and in vivo activity of Lactobacillus sakei L14 strain against Campylobacter jejuni DC3 strain

John Roybert P. Catacutan

John Roybert P. Catacutan

Institute of Biology, National Science Complex, College of Science University of the Philippines DilimanQuezon City, Philippines
Recherchez cet auteur sur
Sciendo|Google Scholar
Catacutan, John Roybert P.
, 
Ma. Socorro Edden P. Subejano

Ma. Socorro Edden P. Subejano

Institute of Biology, National Science Complex, College of Science University of the Philippines DilimanQuezon City, Philippines
Recherchez cet auteur sur
Sciendo|Google Scholar
Subejano, Ma. Socorro Edden P.
 et 
Gil M. Penuliar

Gil M. Penuliar

Institute of Biology, National Science Complex, College of Science University of the Philippines DilimanQuezon City, Philippines
Recherchez cet auteur sur
Sciendo|Google Scholar
Penuliar, Gil M.
  
25 mars 2022
In vitro and in vivo activity of Lactobacillus sakei L14 strain against Campylobacter jejuni DC3 strain's Cover Image
À propos de cet article
Article précédent
Article suivant
Citez
Télécharger la couverture
Publié en ligne: 25 mars 2022
Pages: 85 - 94
Reçu: 12 sept. 2021
Accepté: 06 mars 2022
DOI: https://doi.org/10.2478/jvetres-2022-0015
Mots clés
© 2022 J.R.P. Catacutan et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Domestic poultry is a natural reservoir of Campylobacter, the host–pathogen interaction being predominantly asymptomatic. This study investigated whether chickens remain asymptomatic partly because of lactic acid bacteria (LAB).

Material and Methods

Campylobacter spp. and LAB were isolated from the gut of poultry chickens using enrichment and screening assays and were identified via rDNA sequencing. The C. jejuni DC3 isolate was grown in different cell-free supernatants (CFS) generated from a priority LAB isolate. An in vivo challenge involving the C. jejuni and LAB isolates using a chicken model was performed to confirm the in vitro findings.

Results

Twelve presumptive LAB isolates had anti-C. jejuni activity based on cross-streak and agar plug assays, with Lactobacillus sakei L14 isolate exhibiting the highest activity. Inhibition by L. sakei L14 CFS of the growth of C. jejuni occurred in a dose-dependent manner. Campylobacter jejuni DC3 inhibition was most evident in CFS harvested at 72 h and produced by co-culture with the pathogen. Neutralisation of the CFS abrogated the observed inhibition. Co-infection with C. jejuni DC3 and L. sakei L14 in vivo, however, failed to inhibit C. jejuni colonisation in chickens.

Conclusion

The results suggest that the anti-C. jejuni effect of L. sakei L14 in chickens may be due to mechanisms other than direct inhibition of growth.
Langue:
Anglais
Périodicité:
4 fois par an
Sujets de la revue:
Sciences de la vie, Biologie moléculaire, Microbiologie et virologie, Sciences de la vie, autres, Médecine, Médecine vétérinaire
RSS Feed de la revue