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KDM3A, a Novel Blood-Based Biomarker in Colorectal Carcinogenesis

D. Polat

D. Polat

Department of Biology, Section of Molecular Biology, Faculty of Art and Science, Gaziantep UniversityGaziantep, Turkey
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Polat, D.
, 
E. Onur

E. Onur

Department of Medical Biology, Faculty of Medicine, SANKO UniversityGaziantep, Turkey
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Onur, E.
, 
N. Yılmaz

N. Yılmaz

Department of Internal Medicine, Division of Gastroenterology, Faculty of Medicine, SANKO UniversityGaziantep, Turkey
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Yılmaz, N.
, 
M. Sökücü

M. Sökücü

Department of Pathology, Faculty of Medicine, SANKO UniversityGaziantep, Turkey
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Sökücü, M.
 oraz 
O.F. Gerçeker

O.F. Gerçeker

Department of Biology, Section of Molecular Biology, Faculty of Art and Science, Gaziantep UniversityGaziantep, Turkey
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Gerçeker, O.F.
  
02 maj 2023
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Kategoria artykułu: ORIGINAL ARTICLE
Data publikacji: 02 maj 2023
Zakres stron: 23 - 27
DOI: https://doi.org/10.2478/bjmg-2022-0021
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© 2022 Polat D. et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1.

A-C. Box plot of RT-PCR results. The box border represents the interquartile range, the horizontal line in the box is the median, and circles represent outliers. Values are expressed as log2(2-∆∆CT). Significant differences between control and CRC of HIF-1α (A), KDM3A (B), Slug (C), ZEB-1 (D) genes were shown.
A-C. Box plot of RT-PCR results. The box border represents the interquartile range, the horizontal line in the box is the median, and circles represent outliers. Values are expressed as log2(2-∆∆CT). Significant differences between control and CRC of HIF-1α (A), KDM3A (B), Slug (C), ZEB-1 (D) genes were shown.

Figure 2.

ROC curve, comparing control and CRC cases using KDM3A expression levels
ROC curve, comparing control and CRC cases using KDM3A expression levels

The detailed PCR primers of each gene

Gene Forward Primer Reverse Primer
HIF-1α 5′-TCCATGTGACCATGAGGAAA-3′ 5′-CC AAGCAGGTCATAGGTGGT-3′
KDM3A 5′-GGCGGACTTTAGACGTTCCA-3′ 5′-AGATGAGCCTTCCACTTGGC-3′
E-cadherin 5′-CGGGAATGCAGTTGAGGATC-3′ 5′-AGGATGGTGTAAGCGATGGC-3′
Claudin-1 5′-GATAGCAATCTTTGTGGCCACCGT-3′ 5′-TTCGTACCTGGCATTGACTGGG-3′
Slug 5′-CATGCCTGTCATACCACAAC-3′ 5′-GGTGTCAGATGGAGGAGGG-3′
ZEB-1 5′- GCCAATAAGCAAACGATTCTG-3′ 5′- TTTGGCTGGATCACTTTCAAG-3′
ACTB 5′-GCCGGGACCTGACTGACTAC-3′ 5′-TTCTCCTTAATGTCACGCACGAT-3′

Comparing the control and CRC groups gene expression levels

  Control CRC  
Log2(2-ΔΔCT) Median [%25-%75] Median [%25-%75] p
HIF-1α -1.32 [-5.19- 4.59] 4.25 [0.72 -7.4] 0.0045
KDM3A 0.06 [-3.56-3.12] 4.29 [-0.74-5.97] 0.0049
E-cadherin -1.19 [-4.40-4.03] 0.46 [-2.89-4.01] 0.189
Slug 0.56 [-3.18-2.64] 2.74 [0.75-3.51] 0.008
ZEB-1 0.65 [-2.75-2.18] 2.49 [-1.09-3.58] 0.029
Log2(2-ΔΔCT) Mean±SD Mean±SD p
Claudin-1 0.00058±3.45 0.74±4.03 0.42
Język:
Angielski
Częstotliwość wydawania:
2 razy w roku
Dziedziny czasopisma:
Medycyna, Podstawowe nauki medyczne, Podstawowe nauki medyczne, inne
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