<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Colorectal cancer (CRC) is one of the leading causes of cancer-linked deaths globally. The determination of biomarkers is important in the prognosis and treatment of CRC. Previous studies emphasized the relationship between hypoxia and CRC in humans, and there is strong evidence that this process is strongly related to HIF-1. KDM3A is a histone demethylase that could directly bind to HIF-1α, a subunit of HIF-1. This study aimed to reveal whether the expression level of the <italic>KDM3A</italic> gene could be used as a predictor of CRC. The expression levels of <italic>HIF-1α, KDM3A</italic>, and Epithelial-Mesenchymal Transition (EMT) genes were evaluated by qRT-PCR in leukocyte samples of 50 CRC patients in different stages and 50 healthy controls. <italic>HIF-1α</italic> and <italic>KDM3A</italic> expression levels were significantly higher in the CRC group, compared to the controls. <italic>Slug</italic> and <italic>ZEB-1</italic> genes, the mesenchymal markers, showed the same significance pattern between groups. We acquired 0.664 AUC with 54% sensitivity and 85.4% specificity for separating controls from CRC patients by using the <italic>KDM3A</italic> expression levels in ROC analysis. This data support that <italic>KDM3A</italic> could be a novel supplementary biomarker in diagnosis of CRC, which could be noninvasively detected in circulation.</p>
</abstract>

Colorectal cancer (CRC) is one of the leading causes of cancer-linked deaths globally. The determination of biomarkers is important in the prognosis and treatment of CRC. Previous studies emphasized the relationship between hypoxia and CRC in humans, and there is strong evidence that this process is strongly related to HIF-1. KDM3A is a histone demethylase that could directly bind to HIF-1α, a subunit of HIF-1. This study aimed to reveal whether the expression level of the KDM3A gene could be used as a predictor of CRC. The expression levels of HIF-1α, KDM3A, and Epithelial-Mesenchymal Transition (EMT) genes were evaluated by qRT-PCR in leukocyte samples of 50 CRC patients in different stages and 50 healthy controls. HIF-1α and KDM3A expression levels were significantly higher in the CRC group, compared to the controls. Slug and ZEB-1 genes, the mesenchymal markers, showed the same significance pattern between groups. We acquired 0.664 AUC with 54% sensitivity and 85.4% specificity for separating controls from CRC patients by using the KDM3A expression levels in ROC analysis. This data support that KDM3A could be a novel supplementary biomarker in diagnosis of CRC, which could be noninvasively detected in circulation.

KDM3A, a Novel Blood-Based Biomarker in Colorectal Carcinogenesis

Balkan Journal of Medical Genetics

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"KDM3A, a Novel Blood-Based Biomarker in Colorectal Carcinogenesis"}

Colorectal cancer (CRC) is one of the leading causes of cancer-linked deaths globally. The determination of biomarkers is important in the prognosis and...

Gene	Forward Primer	Reverse Primer
HIF-1α	5′-TCCATGTGACCATGAGGAAA-3′	5′-CC AAGCAGGTCATAGGTGGT-3′
KDM3A	5′-GGCGGACTTTAGACGTTCCA-3′	5′-AGATGAGCCTTCCACTTGGC-3′
E-cadherin	5′-CGGGAATGCAGTTGAGGATC-3′	5′-AGGATGGTGTAAGCGATGGC-3′
Claudin-1	5′-GATAGCAATCTTTGTGGCCACCGT-3′	5′-TTCGTACCTGGCATTGACTGGG-3′
Slug	5′-CATGCCTGTCATACCACAAC-3′	5′-GGTGTCAGATGGAGGAGGG-3′
ZEB-1	5′- GCCAATAAGCAAACGATTCTG-3′	5′- TTTGGCTGGATCACTTTCAAG-3′
ACTB	5′-GCCGGGACCTGACTGACTAC-3′	5′-TTCTCCTTAATGTCACGCACGAT-3′

	Control	CRC
Log₂^(2-ΔΔCT)	Median [%25-%75]	Median [%25-%75]	p
HIF-1α	-1.32 [-5.19- 4.59]	4.25 [0.72 -7.4]	0.0045
KDM3A	0.06 [-3.56-3.12]	4.29 [-0.74-5.97]	0.0049
E-cadherin	-1.19 [-4.40-4.03]	0.46 [-2.89-4.01]	0.189
Slug	0.56 [-3.18-2.64]	2.74 [0.75-3.51]	0.008
ZEB-1	0.65 [-2.75-2.18]	2.49 [-1.09-3.58]	0.029
Log₂^(2-ΔΔCT)	Mean±SD	Mean±SD	p
Claudin-1	0.00058±3.45	0.74±4.03	0.42

KDM3A, a Novel Blood-Based Biomarker in Colorectal Carcinogenesis

Article Category: ORIGINAL ARTICLE

Publié en ligne: 02 mai 2023

Pages: 23 - 27

DOI: https://doi.org/10.2478/bjmg-2022-0021

Mots clés
biomarker, colorectal cancer, qRT-PCR

© 2022 Polat D. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1.

Figure 2.

The detailed PCR primers of each gene

Comparing the control and CRC groups gene expression levels

KDM3A, a Novel Blood-Based Biomarker in Colorectal Carcinogenesis

Article Category: ORIGINAL ARTICLE

Publié en ligne: 02 mai 2023

Pages: 23 - 27

DOI: https://doi.org/10.2478/bjmg-2022-0021

Mots clésbiomarker, colorectal cancer, qRT-PCR

© 2022 Polat D. et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1.

Figure 2.

The detailed PCR primers of each gene

Comparing the control and CRC groups gene expression levels

Mots clés
biomarker, colorectal cancer, qRT-PCR