Open Access

KDM3A, a Novel Blood-Based Biomarker in Colorectal Carcinogenesis

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May 02, 2023

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Figure 1.

A-C. Box plot of RT-PCR results. The box border represents the interquartile range, the horizontal line in the box is the median, and circles represent outliers. Values are expressed as log2(2-∆∆CT). Significant differences between control and CRC of HIF-1α (A), KDM3A (B), Slug (C), ZEB-1 (D) genes were shown.
A-C. Box plot of RT-PCR results. The box border represents the interquartile range, the horizontal line in the box is the median, and circles represent outliers. Values are expressed as log2(2-∆∆CT). Significant differences between control and CRC of HIF-1α (A), KDM3A (B), Slug (C), ZEB-1 (D) genes were shown.

Figure 2.

ROC curve, comparing control and CRC cases using KDM3A expression levels
ROC curve, comparing control and CRC cases using KDM3A expression levels

The detailed PCR primers of each gene

Gene Forward Primer Reverse Primer
HIF-1α 5′-TCCATGTGACCATGAGGAAA-3′ 5′-CC AAGCAGGTCATAGGTGGT-3′
KDM3A 5′-GGCGGACTTTAGACGTTCCA-3′ 5′-AGATGAGCCTTCCACTTGGC-3′
E-cadherin 5′-CGGGAATGCAGTTGAGGATC-3′ 5′-AGGATGGTGTAAGCGATGGC-3′
Claudin-1 5′-GATAGCAATCTTTGTGGCCACCGT-3′ 5′-TTCGTACCTGGCATTGACTGGG-3′
Slug 5′-CATGCCTGTCATACCACAAC-3′ 5′-GGTGTCAGATGGAGGAGGG-3′
ZEB-1 5′- GCCAATAAGCAAACGATTCTG-3′ 5′- TTTGGCTGGATCACTTTCAAG-3′
ACTB 5′-GCCGGGACCTGACTGACTAC-3′ 5′-TTCTCCTTAATGTCACGCACGAT-3′

Comparing the control and CRC groups gene expression levels

  Control CRC  
Log2(2-ΔΔCT) Median [%25-%75] Median [%25-%75] p
HIF-1α -1.32 [-5.19- 4.59] 4.25 [0.72 -7.4] 0.0045
KDM3A 0.06 [-3.56-3.12] 4.29 [-0.74-5.97] 0.0049
E-cadherin -1.19 [-4.40-4.03] 0.46 [-2.89-4.01] 0.189
Slug 0.56 [-3.18-2.64] 2.74 [0.75-3.51] 0.008
ZEB-1 0.65 [-2.75-2.18] 2.49 [-1.09-3.58] 0.029
Log2(2-ΔΔCT) Mean±SD Mean±SD p
Claudin-1 0.00058±3.45 0.74±4.03 0.42
Language:
English
Publication timeframe:
2 times per year
Journal Subjects:
Medicine, Basic Medical Science, Basic Medical Science, other