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In Vitro Effects of Silver Nanoparticles on Pathogenic Bacteria and on Metabolic Activity and Viability of Human Mesenchymal Stem Cells

Maja Ptasiewicz
Maja Ptasiewicz
, 
Renata Chałas
Renata Chałas
, 
Joanna Idaszek
Joanna Idaszek
, 
Paweł Maksymiuk
Paweł Maksymiuk
, 
Mateusz Kister
Mateusz Kister
, 
Karolina A. Kister
Karolina A. Kister
, 
Krzysztof J. Kurzydłowski
Krzysztof J. Kurzydłowski
 oraz 
Agnieszka Magryś
Agnieszka Magryś
   | 29 lut 2024
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Article Category: Original
Data publikacji: 29 lut 2024
Zakres stron: -
Otrzymano: 10 paź 2023
Przyjęty: 30 sty 2024
DOI: https://doi.org/10.2478/aite-2024-0007
Słowa kluczowe
© 2024 Maja Ptasiewicz et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig 1.

Nanoparticles size and shape analysis (A) Representative micro-graphs (TEM) of Ag-NPs provided by the manufacturer (https://www.sigmaaldrich.com/PL/pl/product/aldrich/730785) and (B) computer-processed image for size measurements. (C) Histogram of size distribution function with size defined as an equivalent diameter. (D) Values of a maximum chord plotted against the equivalent diameter for individual particles. (E) Values of a perimeter plotted against the equivalent diameter for individual particles. Ag-NPs, silver nanoparticles.
Nanoparticles size and shape analysis (A) Representative micro-graphs (TEM) of Ag-NPs provided by the manufacturer (https://www.sigmaaldrich.com/PL/pl/product/aldrich/730785) and (B) computer-processed image for size measurements. (C) Histogram of size distribution function with size defined as an equivalent diameter. (D) Values of a maximum chord plotted against the equivalent diameter for individual particles. (E) Values of a perimeter plotted against the equivalent diameter for individual particles. Ag-NPs, silver nanoparticles.

Fig 2.

Metabolic activity of hMSCs incubated with Ag-NPs at a concentration of 0 μg/mL, 3.5 μg/mL, 7 μg/mL, and 14 μg/mL for (A) 10 min, (B) 60 min, and (C) 24 h. *P < 0.05, **P < 0.01, ***P < 0.001. Ag-NPs, silver nanoparticles.
Metabolic activity of hMSCs incubated with Ag-NPs at a concentration of 0 μg/mL, 3.5 μg/mL, 7 μg/mL, and 14 μg/mL for (A) 10 min, (B) 60 min, and (C) 24 h. *P < 0.05, **P < 0.01, ***P < 0.001. Ag-NPs, silver nanoparticles.

Fig 3.

Representative images of hMSC cells stained with acridine orange and propidium iodide: all cells (A) (green); dead cells (red) (B) and overlap of both (C). hMSCs were fixed with MeOH, which caused cell death and membrane permeabilization.
Representative images of hMSC cells stained with acridine orange and propidium iodide: all cells (A) (green); dead cells (red) (B) and overlap of both (C). hMSCs were fixed with MeOH, which caused cell death and membrane permeabilization.

Fig 4.

hMSCs incubated with Ag-NPs at a concertation of 14 μg/mL (A,B); 7 μg/mL (C,D); and 3.5 μg/mL (E,F) for the duration of 24 h. Green color—all cells (a,c,e); Red color—dead cells (b,d,f). Ag-NPs, silver nanoparticles.
hMSCs incubated with Ag-NPs at a concertation of 14 μg/mL (A,B); 7 μg/mL (C,D); and 3.5 μg/mL (E,F) for the duration of 24 h. Green color—all cells (a,c,e); Red color—dead cells (b,d,f). Ag-NPs, silver nanoparticles.

Fig 5.

hMSCs incubated with Ag-NPs at a concertation of 14 μg/mL (A,B); 7 μg/mL (C,D); and 3.5 μg/mL (E,F) for the duration of 1 h. Green color—all cells (a,c,e); Red color—dead cells (b,d,f). Ag-NPs, silver nanoparticles.
hMSCs incubated with Ag-NPs at a concertation of 14 μg/mL (A,B); 7 μg/mL (C,D); and 3.5 μg/mL (E,F) for the duration of 1 h. Green color—all cells (a,c,e); Red color—dead cells (b,d,f). Ag-NPs, silver nanoparticles.

Fig 6.

hMSCs incubated with Ag-NPs at a concertation of 14 μg/mL (A,B); 7 μg/mL (C,D); and 3.5 μg/mL (E,F) for the duration of 24 h. Green color—all cells (a,c,e); Red color—dead cells (b,d,f). Ag-NPs, silver nanoparticles.
hMSCs incubated with Ag-NPs at a concertation of 14 μg/mL (A,B); 7 μg/mL (C,D); and 3.5 μg/mL (E,F) for the duration of 24 h. Green color—all cells (a,c,e); Red color—dead cells (b,d,f). Ag-NPs, silver nanoparticles.

Fig 7.

Antimicrobial activity of Ag-NPs against bacterial strains determined with the well-diffusion method. Bars represent the zones of inhibition of different concentrations of Ag-NPs for the tested bacterial strains. The data represent mean ± SD (n = 3). Ag-NPs, silver nanoparticles; MRSA, methicillin-resistant Staphylococcus aureus; MSSA, methicillin-susceptible Staphylococcus aureus.
Antimicrobial activity of Ag-NPs against bacterial strains determined with the well-diffusion method. Bars represent the zones of inhibition of different concentrations of Ag-NPs for the tested bacterial strains. The data represent mean ± SD (n = 3). Ag-NPs, silver nanoparticles; MRSA, methicillin-resistant Staphylococcus aureus; MSSA, methicillin-susceptible Staphylococcus aureus.

MIC and MBC values (μg/mL) of Ag-NPs for different bacterial strains

Drug-sensitive strains Multidrug-resistant strains
S. aureus MSSA S. mutans E. coli L. acidophilus S. aureus MRSA S. haemolyticus
MIC μg/mL 5 10 5 5 5 5
MBC μg/mL 20 10 20 5 20 5
MBC/MIC ratio 4 1 4 1 4 1
eISSN:
1661-4917
Język:
Angielski
Częstotliwość wydawania:
Volume Open
Dziedziny czasopisma:
Medicine, Basic Medical Science, Biochemistry, Immunology, Clinical Medicine, other, Clinical Chemistry
Kanał RSS czasopisma