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Archivum Immunologiae et Therapiae Experimentalis
Volume 72 (2024): Numero 1 (January 2024)
Accesso libero
In Vitro
Effects of Silver Nanoparticles on Pathogenic Bacteria and on Metabolic Activity and Viability of Human Mesenchymal Stem Cells
Maja Ptasiewicz
Maja Ptasiewicz
,
Renata Chałas
Renata Chałas
,
Joanna Idaszek
Joanna Idaszek
,
Paweł Maksymiuk
Paweł Maksymiuk
,
Mateusz Kister
Mateusz Kister
,
Karolina A. Kister
Karolina A. Kister
,
Krzysztof J. Kurzydłowski
Krzysztof J. Kurzydłowski
e
Agnieszka Magryś
Agnieszka Magryś
| 29 feb 2024
Archivum Immunologiae et Therapiae Experimentalis
Volume 72 (2024): Numero 1 (January 2024)
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Article Category:
Original
Pubblicato online:
29 feb 2024
Pagine:
-
Ricevuto:
10 ott 2023
Accettato:
30 gen 2024
DOI:
https://doi.org/10.2478/aite-2024-0007
Parole chiave
Ag-NPs
,
hMSCs
,
Cytotoxicity
,
Nanoparticles
,
Multi drug-resistant bacteria
© 2024 Maja Ptasiewicz et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Fig 1.
Nanoparticles size and shape analysis (A) Representative micro-graphs (TEM) of Ag-NPs provided by the manufacturer (https://www.sigmaaldrich.com/PL/pl/product/aldrich/730785) and (B) computer-processed image for size measurements. (C) Histogram of size distribution function with size defined as an equivalent diameter. (D) Values of a maximum chord plotted against the equivalent diameter for individual particles. (E) Values of a perimeter plotted against the equivalent diameter for individual particles. Ag-NPs, silver nanoparticles.
Fig 2.
Metabolic activity of hMSCs incubated with Ag-NPs at a concentration of 0 μg/mL, 3.5 μg/mL, 7 μg/mL, and 14 μg/mL for (A) 10 min, (B) 60 min, and (C) 24 h. *P < 0.05, **P < 0.01, ***P < 0.001. Ag-NPs, silver nanoparticles.
Fig 3.
Representative images of hMSC cells stained with acridine orange and propidium iodide: all cells (A) (green); dead cells (red) (B) and overlap of both (C). hMSCs were fixed with MeOH, which caused cell death and membrane permeabilization.
Fig 4.
hMSCs incubated with Ag-NPs at a concertation of 14 μg/mL (A,B); 7 μg/mL (C,D); and 3.5 μg/mL (E,F) for the duration of 24 h. Green color—all cells (a,c,e); Red color—dead cells (b,d,f). Ag-NPs, silver nanoparticles.
Fig 5.
hMSCs incubated with Ag-NPs at a concertation of 14 μg/mL (A,B); 7 μg/mL (C,D); and 3.5 μg/mL (E,F) for the duration of 1 h. Green color—all cells (a,c,e); Red color—dead cells (b,d,f). Ag-NPs, silver nanoparticles.
Fig 6.
hMSCs incubated with Ag-NPs at a concertation of 14 μg/mL (A,B); 7 μg/mL (C,D); and 3.5 μg/mL (E,F) for the duration of 24 h. Green color—all cells (a,c,e); Red color—dead cells (b,d,f). Ag-NPs, silver nanoparticles.
Fig 7.
Antimicrobial activity of Ag-NPs against bacterial strains determined with the well-diffusion method. Bars represent the zones of inhibition of different concentrations of Ag-NPs for the tested bacterial strains. The data represent mean ± SD (n = 3). Ag-NPs, silver nanoparticles; MRSA, methicillin-resistant Staphylococcus aureus; MSSA, methicillin-susceptible Staphylococcus aureus.
MIC and MBC values (μg/mL) of Ag-NPs for different bacterial strains
Drug-sensitive strains
Multidrug-resistant strains
S. aureus
MSSA
S. mutans
E. coli
L. acidophilus
S. aureus
MRSA
S. haemolyticus
MIC μg/mL
5
10
5
5
5
5
MBC μg/mL
20
10
20
5
20
5
MBC/MIC ratio
4
1
4
1
4
1
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