Logowanie
Zarejestruj się
Zresetuj hasło
Publikuj i Dystrybuuj
Rozwiązania Wydawnicze
Rozwiązania Dystrybucyjne
Dziedziny
Architektura i projektowanie
Bibliotekoznawstwo i bibliologia
Biznes i ekonomia
Chemia
Chemia przemysłowa
Filozofia
Fizyka
Historia
Informatyka
Inżynieria
Inżynieria materiałowa
Językoznawstwo i semiotyka
Kulturoznawstwo
Literatura
Matematyka
Medycyna
Muzyka
Nauki farmaceutyczne
Nauki klasyczne i starożytne studia bliskowschodnie
Nauki o Ziemi
Nauki o organizmach żywych
Nauki społeczne
Prawo
Sport i rekreacja
Studia judaistyczne
Sztuka
Teologia i religia
Zagadnienia ogólne
Publikacje
Czasopisma
Książki
Materiały konferencyjne
Wydawcy
Blog
Kontakt
Wyszukiwanie
EUR
USD
GBP
Polski
English
Deutsch
Polski
Español
Français
Italiano
Koszyk
Home
Czasopisma
Asian Biomedicine
Tom 12 (2018): Zeszyt 4 (August 2018)
Otwarty dostęp
Behavioral and histopathological studies of cervical spinal cord contusion injury in rats caused by an adapted weight-drop device
Kanyaratana Bamrungsuk
Kanyaratana Bamrungsuk
,
Anchalee Vattarakorn
Anchalee Vattarakorn
,
Namphung Thongta
Namphung Thongta
,
Kanokwan Tilokskulchai
Kanokwan Tilokskulchai
,
Sompol Tapechum
Sompol Tapechum
oraz
Supin Chompoopong
Supin Chompoopong
| 25 wrz 2019
Asian Biomedicine
Tom 12 (2018): Zeszyt 4 (August 2018)
O artykule
Poprzedni artykuł
Następny artykuł
Abstrakt
Artykuł
Ilustracje i tabele
Referencje
Autorzy
Artykuły w tym zeszycie
Podgląd
PDF
Zacytuj
Udostępnij
Article Category:
Technical report
Data publikacji:
25 wrz 2019
Zakres stron:
189 - 198
DOI:
https://doi.org/10.1515/abm-2019-0019
Słowa kluczowe
animal experimentation
,
contusions
,
motor activity
,
motor skills
,
spinal cord injuries
© 2018 Kanyaratana Bamrungsuk et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.
Figure 1
Adapted weight-drop device used to cause hemicontusion spinal cord injury (A, B). The metal rod was applied to the right side (Rt) of the C5 spinal cord (C, D). The spinal column was stabilized by rigidly clamping the vertebral body at C2 and the spinous process of the C7 vertebra with toothed forceps that were connected to supporting arms of an instrument stand (E)
Figure 2
Forepaw placements were categorized as follows: correct placement, touch, slip, or miss (A). Five types of forepaw placement were recorded when the rats attempted to place the palm of their paw directly onto the rung (B)
Figure 3
Normal forepaw on the contralateral side of the Sprague Dawley rats (A) and clubbed forepaw on the ipsilateral side (B) after right side hemicontusion of the spinal cord at the level of the C5 vertebra
Figure 4
Comparison of adhesive paper (sticker) removal time. For the rats with MSCI (open circles, dashed line) and SSCIS (inverted solid triangles, unbroken line), the time for removing the sticker was increased and there was a significant somatosensory deficit only on days 3 and 7, but not after day 14 when compared with rats in the sham (laminectomy only) group (solid circles, dashed and dotted line). *P < 0.05 when compared to day 1. #P < 0.05 when compared with the SH group. Error bars indicate standard error of the mean
Figure 5
Effect of injury severity on skilled locomotion. The forepaw placement (arrows) is presented as correct placement (A), slip (B), and miss (C). The error scored (D) and the percentage of total rungs used (E) increased significantly on day 3 in rats with MSCI (open circles, dashed lines) and SSCI (inverted solid triangles, dashed and dotted lines). Rats with SSCI showed a significantly higher error and number of rungs used on all days tested compared with rats in the SH group (solid circles, unbroken lines). The percentage of correct forelimb placement (F) by rats with MSCI and SSCI decreased significantly on day 3. Rats with SSCI showed significantly less correct placement than rats with MSCI or SH. *P < 0.05 when compared with day 1. #P < 0.05 compared with rats in the SH group. Error bars indicate standard error of the mean
Figure 6
Distributions of types of forepaw placement expressed as percentage on each day. Rats in the sham injury group (laminectomy only) placed their paws 100% normally or Type I (solid circles, unbroken line) on all days of the experiment (A). Rats with mild spinal cord injury (MSCI) showed recovered placement or Type II (open circles, dotted line) by day 7 and more frequent recovered placement than rats with severe spinal cord injury (SSCI) (B). Rats with SSCI showed forearm placement or Type V (solid square, long dashed line) on day 3 and more frequently than in rats with MSCI (C). Type III (inverted solid triangle, short dashed line), Type IV (open triangle, dotted and dashed line). Error bars indicate standard error of the mean
Figure 7
Comparison of histopathological changes demonstrated by Luxol Fast Blue counterstained with hematoxylin and eosin in the rats with sham (laminectomy only) injury (SH), mild spinal cord injury (MSCI), and severe SCI (SSCI). The transverse sections of the spinal cord at C5 showing the dorsal horn (A), white matter (B), and ventral horn (C). MSCI and SSCI showing an intact dura mater and external surface the spinal tissue. The cystic cavity (asterisks) in rats with SSCI was larger than that in rats with MSCI. Scale bars represent 400 μm
Podgląd