Login
Registrati
Reimposta password
Pubblica & Distribuisci
Soluzioni Editoriali
Soluzioni di Distribuzione
Temi
Architettura e design
Arti
Business e Economia
Chimica
Chimica industriale
Farmacia
Filosofia
Fisica
Geoscienze
Ingegneria
Interesse generale
Legge
Letteratura
Linguistica e semiotica
Matematica
Medicina
Musica
Scienze bibliotecarie e dell'informazione, studi library
Scienze dei materiali
Scienze della vita
Scienze informatiche
Scienze sociali
Sport e tempo libero
Storia
Studi classici e del Vicino Oriente antico
Studi culturali
Studi ebraici
Teologia e religione
Pubblicazioni
Riviste
Libri
Atti
Editori
Blog
Contatti
Cerca
EUR
USD
GBP
Italiano
English
Deutsch
Polski
Español
Français
Italiano
Carrello
Home
Riviste
Asian Biomedicine
Volume 12 (2018): Numero 4 (August 2018)
Accesso libero
Behavioral and histopathological studies of cervical spinal cord contusion injury in rats caused by an adapted weight-drop device
Kanyaratana Bamrungsuk
Kanyaratana Bamrungsuk
,
Anchalee Vattarakorn
Anchalee Vattarakorn
,
Namphung Thongta
Namphung Thongta
,
Kanokwan Tilokskulchai
Kanokwan Tilokskulchai
,
Sompol Tapechum
Sompol Tapechum
e
Supin Chompoopong
Supin Chompoopong
| 25 set 2019
Asian Biomedicine
Volume 12 (2018): Numero 4 (August 2018)
INFORMAZIONI SU QUESTO ARTICOLO
Articolo precedente
Articolo Successivo
Sommario
Articolo
Immagini e tabelle
Bibliografia
Autori
Articoli in questo Numero
Anteprima
PDF
Cita
CONDIVIDI
Article Category:
Technical report
Pubblicato online:
25 set 2019
Pagine:
189 - 198
DOI:
https://doi.org/10.1515/abm-2019-0019
Parole chiave
animal experimentation
,
contusions
,
motor activity
,
motor skills
,
spinal cord injuries
© 2018 Kanyaratana Bamrungsuk et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.
Figure 1
Adapted weight-drop device used to cause hemicontusion spinal cord injury (A, B). The metal rod was applied to the right side (Rt) of the C5 spinal cord (C, D). The spinal column was stabilized by rigidly clamping the vertebral body at C2 and the spinous process of the C7 vertebra with toothed forceps that were connected to supporting arms of an instrument stand (E)
Figure 2
Forepaw placements were categorized as follows: correct placement, touch, slip, or miss (A). Five types of forepaw placement were recorded when the rats attempted to place the palm of their paw directly onto the rung (B)
Figure 3
Normal forepaw on the contralateral side of the Sprague Dawley rats (A) and clubbed forepaw on the ipsilateral side (B) after right side hemicontusion of the spinal cord at the level of the C5 vertebra
Figure 4
Comparison of adhesive paper (sticker) removal time. For the rats with MSCI (open circles, dashed line) and SSCIS (inverted solid triangles, unbroken line), the time for removing the sticker was increased and there was a significant somatosensory deficit only on days 3 and 7, but not after day 14 when compared with rats in the sham (laminectomy only) group (solid circles, dashed and dotted line). *P < 0.05 when compared to day 1. #P < 0.05 when compared with the SH group. Error bars indicate standard error of the mean
Figure 5
Effect of injury severity on skilled locomotion. The forepaw placement (arrows) is presented as correct placement (A), slip (B), and miss (C). The error scored (D) and the percentage of total rungs used (E) increased significantly on day 3 in rats with MSCI (open circles, dashed lines) and SSCI (inverted solid triangles, dashed and dotted lines). Rats with SSCI showed a significantly higher error and number of rungs used on all days tested compared with rats in the SH group (solid circles, unbroken lines). The percentage of correct forelimb placement (F) by rats with MSCI and SSCI decreased significantly on day 3. Rats with SSCI showed significantly less correct placement than rats with MSCI or SH. *P < 0.05 when compared with day 1. #P < 0.05 compared with rats in the SH group. Error bars indicate standard error of the mean
Figure 6
Distributions of types of forepaw placement expressed as percentage on each day. Rats in the sham injury group (laminectomy only) placed their paws 100% normally or Type I (solid circles, unbroken line) on all days of the experiment (A). Rats with mild spinal cord injury (MSCI) showed recovered placement or Type II (open circles, dotted line) by day 7 and more frequent recovered placement than rats with severe spinal cord injury (SSCI) (B). Rats with SSCI showed forearm placement or Type V (solid square, long dashed line) on day 3 and more frequently than in rats with MSCI (C). Type III (inverted solid triangle, short dashed line), Type IV (open triangle, dotted and dashed line). Error bars indicate standard error of the mean
Figure 7
Comparison of histopathological changes demonstrated by Luxol Fast Blue counterstained with hematoxylin and eosin in the rats with sham (laminectomy only) injury (SH), mild spinal cord injury (MSCI), and severe SCI (SSCI). The transverse sections of the spinal cord at C5 showing the dorsal horn (A), white matter (B), and ventral horn (C). MSCI and SSCI showing an intact dura mater and external surface the spinal tissue. The cystic cavity (asterisks) in rats with SSCI was larger than that in rats with MSCI. Scale bars represent 400 μm
Anteprima