<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Plants are known to possess enormous profound benefits, which if well incorporated in daily living have the potential to influence our health in ways unexpected. Significant progress has been made concerning the anti-oxidative and anti-cancerous effect of bioactive components in food products, worthy of note in the curcuminoids derived from turmeric (<italic>Curcuma longa</italic>) rhizomes. Therefore, to this end, this research aims to describe succinctly the phytocomponents, antioxidant activity and anti-microbial activity of turmeric methanol extract. The zones of inhibition were measured after plating on a Mueller Hinton agar plate and compared to the standards penicillin G and chloramphenicol. Chloramphenicol used as a positive control was found to be most effective on <italic>Staphylococcus aureus</italic> while <italic>Curcuma longa</italic> methanol extract and penicillin G were found to have the same microbial inhibition due to the negative control. Compared to the IC50 values of Ascorbic acid which was 5 mg/ ml, Curcuma longa methanol extract had a lower IC50 value is 3.2 mg/ml which according to previous literature is a good one given that lower IC50 values indicate great antioxidant activity. It was gathered by the observation that the qualitative extraction of <italic>Curcuma longa</italic> using methanol shows the presence of varying chemical components such as AR-tumerone, arachinsaeure, alpha-tumerone amongst others. Research data suggest that ar-turmerone has the potential to promote the positive and negative proliferation of tumor cell lines. As a result, <italic>Curcuma longa</italic> has high potential to be developed into an antibiotic against Methicillin-resistant <italic>Staphylococcus aureus</italic> and other clinically important bacterial strains in future.</p></abstract>

Plants are known to possess enormous profound benefits, which if well incorporated in daily living have the potential to influence our health in ways unexpected. Significant progress has been made concerning the anti-oxidative and anti-cancerous effect of bioactive components in food products, worthy of note in the curcuminoids derived from turmeric (Curcuma longa) rhizomes. Therefore, to this end, this research aims to describe succinctly the phytocomponents, antioxidant activity and anti-microbial activity of turmeric methanol extract. The zones of inhibition were measured after plating on a Mueller Hinton agar plate and compared to the standards penicillin G and chloramphenicol. Chloramphenicol used as a positive control was found to be most effective on Staphylococcus aureus while Curcuma longa methanol extract and penicillin G were found to have the same microbial inhibition due to the negative control. Compared to the IC50 values of Ascorbic acid which was 5 mg/ ml, Curcuma longa methanol extract had a lower IC50 value is 3.2 mg/ml which according to previous literature is a good one given that lower IC50 values indicate great antioxidant activity. It was gathered by the observation that the qualitative extraction of Curcuma longa using methanol shows the presence of varying chemical components such as AR-tumerone, arachinsaeure, alpha-tumerone amongst others. Research data suggest that ar-turmerone has the potential to promote the positive and negative proliferation of tumor cell lines. As a result, Curcuma longa has high potential to be developed into an antibiotic against Methicillin-resistant Staphylococcus aureus and other clinically important bacterial strains in future.

Detection of Biological Activity of <italic>Curcuma longa</italic> Methanol Extract and Its Antibacterial effect on <italic>Staphylococcus aureus</italic>

The EuroBiotech Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Detection of Biological Activity of  Methanol Extract and Its Antibacterial effect on 

Plants are known to possess enormous profound benefits, which if well incorporated in daily living have the potential to influence our health in ways...

S/N	Chemical compound name	Retention times (Mins)	Molecular formula
1	4-hydroxy-3-(2-oxo-2h-1-oxa-3-phenanthryl)-2(1h)-quinolone	5.583	C₁₀H₇NO₄
2	1,2,2-Trimethylcyclopropylamine	7.717	C₆H₁₃N
3	Glycine, N-[[(4-methoxyphenyl)methoxy]carbonyl]-N-methyl-	8.717	C₂₅H₂₃NO₆
44	1,E-1,Z-13-Octadecatriene	15,517	C₁₈H₃₂
5	13-Heptadecyn-1-ol (CAS) 1-HYDROXY HEPTADEC-13-YNE	15.942	CHO
6	Oleoylcholesterol	16,267	C₄₅H₇₈O₂
7	1H-Purin-6-amine, [(2-fluorophenyl)methyl]-	16,775	C₁₂H₁₀FN₅
8	AR-TUMERONE	18092	C₁₅H₂₀O
9	ALPHA.-TUMERONE	18642	C₁₅H₂O
10	6,9,12-Octadecatrienoic acid, phenyl methyl ester	23933	C₁₉H₃₂O₂
11	ARACHINSAEURE	24383	C₂₀H₄₀O₂

Microorganisms	Staphylococcus aureus (B-4420)
Concentration (mg/ml)	50	25	12.5	6.25
Methanol turmeric extract	-	-	+	+

Duncan^a CONS	N	Subset for alpha = .05
Duncan^a CONS	N	1	2	3
N (Dimethyl Sulfoxide)	6	5,02
Curcuma longa methanol extract	6		8,07
PEN (Penicillin G)	6		8,21
CHL (Chloramphenicol)	6			12,83
Sig.		1,000	,791	1,000

Detection of Biological Activity of Curcuma longa Methanol Extract and Its Antibacterial effect on Staphylococcus aureus

Article Category: Research Article

Pubblicato online: 22 apr 2021

Pagine: 56 - 62

DOI: https://doi.org/10.2478/ebtj-2021-0009

Parole chiave
antimicrobial activity, antioxidant activity,

© 2021 Covenant Ogbonna, Erkay Özgör, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Figure 2

Curcuma longa extracts and standards showing IC50 values

Showing the GC-MS profiling of Curcuma longa methanol extract

Showing the minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) for Staphylococcus aureus

Showing the zone of inhibition for Staphylococcus aureus by Duncan test

Samples analyzed	IC50 values
Curcuma longa methanol extract	3.2 mg/ml
Ascorbic acid standard	5 mg/ml

Detection of Biological Activity of Curcuma longa Methanol Extract and Its Antibacterial effect on Staphylococcus aureus

Article Category: Research Article

Pubblicato online: 22 apr 2021

Pagine: 56 - 62

DOI: https://doi.org/10.2478/ebtj-2021-0009

Parole chiaveantimicrobial activity, antioxidant activity,

© 2021 Covenant Ogbonna, Erkay Özgör, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Figure 2

Curcuma longa extracts and standards showing IC50 values

Showing the GC-MS profiling of Curcuma longa methanol extract

Showing the minimum inhibitory concentrations (MIC) and minimum bactericidal concentration (MBC) for Staphylococcus aureus

Showing the zone of inhibition for Staphylococcus aureus by Duncan test

Parole chiave
antimicrobial activity, antioxidant activity,