Connexion
S'inscrire
Réinitialiser le mot de passe
Publier & Distribuer
Solutions d'édition
Solutions de distribution
Thèmes
Architecture et design
Arts
Business et économie
Chimie
Chimie industrielle
Droit
Géosciences
Histoire
Informatique
Ingénierie
Intérêt général
Linguistique et sémiotique
Littérature
Mathématiques
Musique
Médecine
Pharmacie
Philosophie
Physique
Sciences bibliothécaires et de l'information, études du livre
Sciences des matériaux
Sciences du vivant
Sciences sociales
Sport et loisirs
Théologie et religion
Études classiques et du Proche-Orient ancient
Études culturelles
Études juives
Publications
Journaux
Livres
Comptes-rendus
Éditeurs
Blog
Contact
Chercher
EUR
USD
GBP
Français
English
Deutsch
Polski
Español
Français
Italiano
Panier
Home
Journaux
Archives of Industrial Hygiene and Toxicology
Édition 71 (2020): Edition 2 (June 2020)
Accès libre
Superior protective effects of
in vitro
propagated green garlic against hydrogen peroxide-induced cytotoxicity in human hepatoma cells
Tomislav Vinković
Tomislav Vinković
,
Nada Parađiković
Nada Parađiković
,
Monika Tkalec Kojić
Monika Tkalec Kojić
,
Gordana Mendaš
Gordana Mendaš
,
Tanja Živković Semren
Tanja Živković Semren
,
Valentina Gluščić
Valentina Gluščić
,
Ivana Vinković Vrček
Ivana Vinković Vrček
et
Ivan Pavičić
Ivan Pavičić
| 29 juin 2020
Archives of Industrial Hygiene and Toxicology
Édition 71 (2020): Edition 2 (June 2020)
À propos de cet article
Article précédent
Article suivant
Résumé
Article
Figures et tableaux
Références
Auteurs
Articles dans cette édition
Aperçu
PDF
Citez
Partagez
Article Category:
Original article
Publié en ligne:
29 juin 2020
Pages:
130 - 137
Reçu:
01 févr. 2019
Accepté:
01 juin 2020
DOI:
https://doi.org/10.2478/aiht-2020-71-3399
Mots clés
L.
,
alliin
,
antioxidant activity
,
glutathione
,
total phenolic content
,
total flavonoid content
© 2020 Tomislav Vinković et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Figure 1
Total garlic extract phenolic content (expressed as mg of GAE per gram of garlic powder) and antioxidant activity (AOX, expressed as mm of TEAC per gram of garlic powder). Values represent means ± standard deviations. Different letters denote significant differences (P<0.05) between CG and PG. GAE – gallic acid equivalent; TEAC – Trolox equivalent antioxidant activity
Figure 2
Total flavonoid and alliin content (expressed as milligrams of quercetin and alliin per gram of garlic powder, respectively). Values represent means ± standard deviations. Different letters denote significant differences (P<0.05) between CG and PG
Figure 3
Hepatoma cell viability according to the WST-8 colorimetric assay. Data are expressed as a percentage of absorbance with respect to negative control as a starting point (100 %). Values represent means ± standard deviations. Values marked with the same letters are not significantly different, whereas values marked with different letters are significantly different. # indicates significant difference from negative control and * from positive (H2O2) control (P<0.05)
Figure 4
GSH levels determined with the MBCI fluorescent probe. Data are expressed as a percentage of fluorescence of negative control as a starting point (100 %). Values represent means ± standard deviations. Values marked with the same letters are not significantly different, whereas values marked with different letters are significantly different. # indicates significant difference from negative control and * from positive (H2O2) control (P<0.05)
Figure 5
Intracellular ROS levels determined with the DCFH-DA fluorescent probe. Data are expressed as a percentage of fluorescence of negative control as a starting point (100 %). Values represent means ± standard deviations. Values marked with the same letters are not significantly different, whereas values marked with different letters are significantly different. # indicates significant difference from negative control and * from positive (H2O2) control (P<0.05)
Differences in yield and overall alliin content between conventional growing and in vitro propagation
Garlic PT
Leaves number
Yield per plant (bulbs or leafy part) (g)
Yield per square meter (g)
Alliin yield per square meter (g)
CG
12.57±0.89
a
38.69±0.73
a
1102.77±20.89
b
9.02±0.15
b
PG
9.89±0.66
b
30.57±0.42
b
3056.98±42.14
a
74.79±0.94
a
Aperçu