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Journals
Archives of Industrial Hygiene and Toxicology
Volume 71 (2020): Issue 2 (June 2020)
Open Access
Superior protective effects of
in vitro
propagated green garlic against hydrogen peroxide-induced cytotoxicity in human hepatoma cells
Tomislav Vinković
Tomislav Vinković
,
Nada Parađiković
Nada Parađiković
,
Monika Tkalec Kojić
Monika Tkalec Kojić
,
Gordana Mendaš
Gordana Mendaš
,
Tanja Živković Semren
Tanja Živković Semren
,
Valentina Gluščić
Valentina Gluščić
,
Ivana Vinković Vrček
Ivana Vinković Vrček
and
Ivan Pavičić
Ivan Pavičić
| Jun 29, 2020
Archives of Industrial Hygiene and Toxicology
Volume 71 (2020): Issue 2 (June 2020)
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Article Category:
Original article
Published Online:
Jun 29, 2020
Page range:
130 - 137
Received:
Feb 01, 2019
Accepted:
Jun 01, 2020
DOI:
https://doi.org/10.2478/aiht-2020-71-3399
Keywords
L.
,
alliin
,
antioxidant activity
,
glutathione
,
total phenolic content
,
total flavonoid content
© 2020 Tomislav Vinković et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Figure 1
Total garlic extract phenolic content (expressed as mg of GAE per gram of garlic powder) and antioxidant activity (AOX, expressed as mm of TEAC per gram of garlic powder). Values represent means ± standard deviations. Different letters denote significant differences (P<0.05) between CG and PG. GAE – gallic acid equivalent; TEAC – Trolox equivalent antioxidant activity
Figure 2
Total flavonoid and alliin content (expressed as milligrams of quercetin and alliin per gram of garlic powder, respectively). Values represent means ± standard deviations. Different letters denote significant differences (P<0.05) between CG and PG
Figure 3
Hepatoma cell viability according to the WST-8 colorimetric assay. Data are expressed as a percentage of absorbance with respect to negative control as a starting point (100 %). Values represent means ± standard deviations. Values marked with the same letters are not significantly different, whereas values marked with different letters are significantly different. # indicates significant difference from negative control and * from positive (H2O2) control (P<0.05)
Figure 4
GSH levels determined with the MBCI fluorescent probe. Data are expressed as a percentage of fluorescence of negative control as a starting point (100 %). Values represent means ± standard deviations. Values marked with the same letters are not significantly different, whereas values marked with different letters are significantly different. # indicates significant difference from negative control and * from positive (H2O2) control (P<0.05)
Figure 5
Intracellular ROS levels determined with the DCFH-DA fluorescent probe. Data are expressed as a percentage of fluorescence of negative control as a starting point (100 %). Values represent means ± standard deviations. Values marked with the same letters are not significantly different, whereas values marked with different letters are significantly different. # indicates significant difference from negative control and * from positive (H2O2) control (P<0.05)
Differences in yield and overall alliin content between conventional growing and in vitro propagation
Garlic PT
Leaves number
Yield per plant (bulbs or leafy part) (g)
Yield per square meter (g)
Alliin yield per square meter (g)
CG
12.57±0.89
a
38.69±0.73
a
1102.77±20.89
b
9.02±0.15
b
PG
9.89±0.66
b
30.57±0.42
b
3056.98±42.14
a
74.79±0.94
a
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