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DNA Cleavage and Cytotoxic Activity of Copper(II) Complexes Based on Reduced Schiff Bases Derived From Salicylaldehyde and Amino Acids

Jana Korcová
Jana Korcová
, 
Mária Húserková
Mária Húserková
, 
Lucia Lintnerová
Lucia Lintnerová
 et 
Jindra Valentová
Jindra Valentová
   | 28 mars 2024
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Article Category: Short communication
Publié en ligne: 28 mars 2024
Pages: 48 - 51
Reçu: 09 oct. 2023
Accepté: 15 févr. 2024
DOI: https://doi.org/10.2478/afpuc-2024-0003
Mots clés
© 2024 Jana Korcová et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

DNA cleavage activity of LL2 complex after 5h/37°C incubationLine 1: C- negative control (pBR 322 in TRIS-HCl, pH 7.5)Line 2-4: LL2 in the concentration of 1×10−3, 3×10−3, 5×10−3 added to pBR 322.
DNA cleavage activity of LL2 complex after 5h/37°C incubationLine 1: C- negative control (pBR 322 in TRIS-HCl, pH 7.5)Line 2-4: LL2 in the concentration of 1×10−3, 3×10−3, 5×10−3 added to pBR 322.

Fig. 2.

DNA cleavage activity of LL3 complex after 5h/37°C incubationLine 1: C- negative control (pBR 322 in TRIS-HCl, pH 7.5)Line 2-4: LL3 in the concentration of 1×10−3, 3×10−3, 5×10−3 added to pBR 322.
DNA cleavage activity of LL3 complex after 5h/37°C incubationLine 1: C- negative control (pBR 322 in TRIS-HCl, pH 7.5)Line 2-4: LL3 in the concentration of 1×10−3, 3×10−3, 5×10−3 added to pBR 322.

Fig. 3.

DNA cleavage activity of LL4 complex after 5h/37°C incubationLine 1: C- negative control (pBR 322 in TRIS-HCl, pH 7.5)Line 2-4: LL4 in the concentration of 1×10−3, 3×10−3, 5×10−3 added to pBR 322.
DNA cleavage activity of LL4 complex after 5h/37°C incubationLine 1: C- negative control (pBR 322 in TRIS-HCl, pH 7.5)Line 2-4: LL4 in the concentration of 1×10−3, 3×10−3, 5×10−3 added to pBR 322.

Fig. 4.

DNA cleavage activity of LL5 complex after 5h/37°C incubationLine 1: C- negative control (pBR 322 in TRIS-HCl, pH 7.5)Line 2-4: LL5 in the concentration of 1×10−3, 3×10−3, 5×10−3 added to pBR 322.
DNA cleavage activity of LL5 complex after 5h/37°C incubationLine 1: C- negative control (pBR 322 in TRIS-HCl, pH 7.5)Line 2-4: LL5 in the concentration of 1×10−3, 3×10−3, 5×10−3 added to pBR 322.

Figure 5.

Cytotoxic activity of copper(II) complexes. Expressed as a percentage of the amount of untransformed resazurin to resorufin after 3 h of incubation with copper(II) complexes. Comparison with C+ control was performed by t-test Significance levels: ***0.000 < P < 0.001; **0.001 < P < 0.01; *0.01 < P < 0.05
Cytotoxic activity of copper(II) complexes. Expressed as a percentage of the amount of untransformed resazurin to resorufin after 3 h of incubation with copper(II) complexes. Comparison with C+ control was performed by t-test Significance levels: ***0.000 < P < 0.001; **0.001 < P < 0.01; *0.01 < P < 0.05

j.afpuc-2024-0003.tab.001

Wavelenght Resorufin ɛRED Resazurin ɛOX
570 nm 155,677 80,586
600 nm 14,652 117,216
eISSN:
2453-6725
Langue:
Anglais
Périodicité:
2 fois par an
Sujets de la revue:
Pharmacy, other
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