Acceso abierto

Comparative Toxicity of Lunar, Martian Dust Simulants, and Urban Dust in Human Skin Fibroblast Cells

James T.F. Wise

James T.F. Wise

Wise Laboratory of Environmental and Genetic Toxicology, University of Southern MainePortland, ME, United States
Buscar este autor en
Sciendo|Google Scholar
Wise, James T.F.
, 
Hong Xie

Hong Xie

Wise Laboratory of Environmental and Genetic Toxicology, University of Southern MainePortland, ME, United States
Buscar este autor en
Sciendo|Google Scholar
Xie, Hong
, 
John Pierce Wise

John Pierce Wise

Wise Laboratory of Environmental and Genetic Toxicology, University of Southern MainePortland, ME, United States
Buscar este autor en
Sciendo|Google Scholar
Wise, John Pierce
, 
Michael Mason

Michael Mason

Department of Chemical and Biological Engineering, University of MaineOrono, ME, United States
Buscar este autor en
Sciendo|Google Scholar
Mason, Michael
, 
Antony Jeevarajan

Antony Jeevarajan

NASA Johnson Space CenterHouston, TX, United States
Buscar este autor en
Sciendo|Google Scholar
Jeevarajan, Antony
, 
William Wallace

William Wallace

Wyle Science, Technology, and Engineering GroupHouston, TX, United States
Buscar este autor en
Sciendo|Google Scholar
Wallace, William
 y 
John Pierce Wise

John Pierce Wise

Wise Laboratory of Environmental and Genetic Toxicology, University of Southern MainePortland, ME, United States
Buscar este autor en
Sciendo|Google Scholar
Wise, John Pierce
  
01 jul 2015
Comparative Toxicity of Lunar, Martian Dust Simulants, and Urban Dust in Human Skin Fibroblast Cells's Cover Image
Acerca de este artículo
Artículo anterior
Artículo siguiente
Cite
Descargar portada
Categoría del artículo: Research Article
Publicado en línea: 01 jul 2015
Páginas: 51 - 58
DOI: https://doi.org/10.2478/gsr-2015-0005
Palabras clave
© 2015 James T.F. Wise et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1.

Cytotoxicity of the Four Dusts to Human Skin Fibroblasts after 24 h Exposure. This figure shows lunar dust simulants, Mars dust simulant, and urban dust are cytotoxic to BJhTERT cells after a 24 hour exposure. The very fine lunar dust simulant and the Mars dust simulant were the most cytotoxic. For JSC-1A-f, concentrations of 50, 100, 200, and 400 μg/cm2 are statistically different from control (p<0.05). For JSC-1A-vf, concentrations of 25, 100, 200, and 400 μg/cm2 are statistically different from control (p<0.05). For Mars-1A, concentrations of 25, 50, 100, 200, and 400 μg/cm2 are statistically different from control (p<0.05). For urban dust, concentrations of 50, 100, 200, and 400 μg/cm2 are statistically different from control (p<0.05). Data represent an average of three experiments ± standard error of mean.
Cytotoxicity of the Four Dusts to Human Skin Fibroblasts after 24 h Exposure. This figure shows lunar dust simulants, Mars dust simulant, and urban dust are cytotoxic to BJhTERT cells after a 24 hour exposure. The very fine lunar dust simulant and the Mars dust simulant were the most cytotoxic. For JSC-1A-f, concentrations of 50, 100, 200, and 400 μg/cm2 are statistically different from control (p<0.05). For JSC-1A-vf, concentrations of 25, 100, 200, and 400 μg/cm2 are statistically different from control (p<0.05). For Mars-1A, concentrations of 25, 50, 100, 200, and 400 μg/cm2 are statistically different from control (p<0.05). For urban dust, concentrations of 50, 100, 200, and 400 μg/cm2 are statistically different from control (p<0.05). Data represent an average of three experiments ± standard error of mean.

Figure 2.

Cytotoxicity of the Four Dusts to Human Skin Fibroblasts after 120 h Exposure. This figure shows lunar dust simulants, Mars dust simulant, and urban dust are cytotoxic to BJhTERT cells after a 120 hour exposure. The very fine lunar dust simulant and the Mars dust simulant are the most cytotoxic. For JSC-1A-f, concentrations of 100, 200, and 400 μg/cm2 are statistically different from control (p<0.05). For JSC-1A-vf, concentrations of 100, 200, and 400 μg/cm2 are statistically different from control (p<0.001). For Mars-1A, concentrations of 25, 50, and 100 μg/cm2 are statistically different from control (p< .05). For urban dust, concentrations of 50, 100, 200, and 400 μg/cm2 are statistically different from control (p<0.05). Data represent an average of three experiments ± standard error of mean.
Cytotoxicity of the Four Dusts to Human Skin Fibroblasts after 120 h Exposure. This figure shows lunar dust simulants, Mars dust simulant, and urban dust are cytotoxic to BJhTERT cells after a 120 hour exposure. The very fine lunar dust simulant and the Mars dust simulant are the most cytotoxic. For JSC-1A-f, concentrations of 100, 200, and 400 μg/cm2 are statistically different from control (p<0.05). For JSC-1A-vf, concentrations of 100, 200, and 400 μg/cm2 are statistically different from control (p<0.001). For Mars-1A, concentrations of 25, 50, and 100 μg/cm2 are statistically different from control (p< .05). For urban dust, concentrations of 50, 100, 200, and 400 μg/cm2 are statistically different from control (p<0.05). Data represent an average of three experiments ± standard error of mean.

Figure 3.

Chromosome Damage of the Four Dusts to Human Skin Fibroblasts after 120 h Exposure. This figure shows that lunar dust simulants, Mars dust simulant, and urban dust do not significantly increase chromosome damage after a 120 hour exposure. Data represent an average of 3 experiments ± standard error of mean. Panel (A) shows the percent of metaphases with damage. Panel (B) shows the total chromosome damage in 100 metaphases. * Indicates no metaphases (50 and 100 μg/cm2 for JSC-1A-vf and 100 μg/cm2 for Mars-1A).
Chromosome Damage of the Four Dusts to Human Skin Fibroblasts after 120 h Exposure. This figure shows that lunar dust simulants, Mars dust simulant, and urban dust do not significantly increase chromosome damage after a 120 hour exposure. Data represent an average of 3 experiments ± standard error of mean. Panel (A) shows the percent of metaphases with damage. Panel (B) shows the total chromosome damage in 100 metaphases. * Indicates no metaphases (50 and 100 μg/cm2 for JSC-1A-vf and 100 μg/cm2 for Mars-1A).

Mass median diameters (MMD) of the lunar and Martian dust simulants and urban dust as determined by dynamic light scattering_

Chemical MMD (µm)
JSC-1A-f 1.470
JSC-1A-vf 0.726
Urban dust 0.169
Mars-1A 0.133
Idioma:
Inglés
Calendario de la edición:
2 veces al año
Temas de la revista:
Ciencias de la vida, Ciencias de la vida, otros, Ciencia de los materiales, Ciencia de los materiales, otros, Física, Física, otros
RSS Feed de revista