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First report of the presence of enterotoxin gene in coagulase-negative staphylococci recovered from meat of snails (Achatina achatina)

Arthur C. Okafor

Arthur C. Okafor

Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food SafetyVienna, Austria
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Okafor, Arthur C.
, 
Frank C. Ogbo

Frank C. Ogbo

Department of Applied Microbiology & Brewing, Nnamdi Azikiwe UniversityAwka, Nigeria
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Ogbo, Frank C.
, 
Fred C. Akharaiyi

Fred C. Akharaiyi

Department of Microbiology, Edo State University UzairueAuchi, Nigeria
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Akharaiyi, Fred C.
 und 
Henry B. Oladeinde

Henry B. Oladeinde

Department of Medical Laboratory Science, Western Delta UniversityOghara, Nigeria
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Oladeinde, Henry B.
  
07. Juni 2022
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Online veröffentlicht: 07. Juni 2022
Seitenbereich: 161 - 165
Eingereicht: 11. Jan. 2022
Akzeptiert: 27. Mai 2022
DOI: https://doi.org/10.2478/jvetres-2022-0029
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© 2022 A.C. Okafor et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

It has been suggested that coagulase-negative staphylococci can serve as reservoirs of virulence genes for other bacteria. This study assessed the presence of such genes in selected isolates recovered from meat of the giant African snail (Achatina achatina).

Material and Methods

Virulence genes were detected using a polymerase chain reaction targeting specific primers. Two representative isolates were identified using 16S rRNA gene sequencing.

Results

The results showed that the staphylococcal enterotoxin A gene (sea) was present in five out of the eight isolates studied. The isolates expressed resistance mainly to three antibiotics: chloramphenicol, norfloxacin and cloxacillin in descending order of incidence. Most importantly, the Staphylococcus sciuri isolate NEDU 181, in addition to being resistant to the three aforementioned antibiotics, also harboured the sea gene.

Conclusion

Our findings demonstrate, for the first time, the presence of toxigenic and antibiotic-resistant coagulase-negative Staphylococcus spp. in commercially-available fresh snail meat. With staphylococcal enterotoxin A known to survive cooking temperature, this presents a food safety concern.
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Englisch
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Biologie, Molekularbiologie, Mikrobiologie und Virologie, Biologie, andere, Medizin, Veterinärmedizin
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