First report of the presence of enterotoxin gene in coagulase-negative staphylococci recovered from meat of snails ()

It has been suggested that coagulase-negative staphylococci can serve as reservoirs of virulence genes for other bacteria. This study assessed the presence of such genes in selected isolates recovered from meat of the giant African snail (Achatina achatina).

Material and Methods

Virulence genes were detected using a polymerase chain reaction targeting specific primers. Two representative isolates were identified using 16S rRNA gene sequencing.

Results

The results showed that the staphylococcal enterotoxin A gene (sea) was present in five out of the eight isolates studied. The isolates expressed resistance mainly to three antibiotics: chloramphenicol, norfloxacin and cloxacillin in descending order of incidence. Most importantly, the Staphylococcus sciuri isolate NEDU 181, in addition to being resistant to the three aforementioned antibiotics, also harboured the sea gene.

Conclusion

Our findings demonstrate, for the first time, the presence of toxigenic and antibiotic-resistant coagulase-negative Staphylococcus spp. in commercially-available fresh snail meat. With staphylococcal enterotoxin A known to survive cooking temperature, this presents a food safety concern.

eISSN:: 2450-8608
Idioma:: Inglés

Calendario de la edición:: 4 veces al año
Temas de la revista:: Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine

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First report of the presence of enterotoxin gene in coagulase-negative staphylococci recovered from meat of snails (Achatina achatina)

Publicado en línea: 07 jun 2022

Páginas: 161 - 165

Recibido: 11 ene 2022

Aceptado: 27 may 2022

DOI: https://doi.org/10.2478/jvetres-2022-0029

Palabras clavefresh snail meat, 16S rRNA gene, gene, antibiotic resistance

© 2022 A.C. Okafor et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Material and Methods

Results

Conclusion

Palabras clave
fresh snail meat, 16S rRNA gene, gene, antibiotic resistance