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Epidemiological Characteristics of Shiga Toxin-Producing Escherichia coli Responsible for Infections in the Polish Pediatric Population

Dominika Seliga-Gąsior
Dominika Seliga-Gąsior
, 
Beata Sokól-Leszczyñska
Beata Sokól-Leszczyñska
, 
Jolanta Krzysztoñ-Russjan
Jolanta Krzysztoñ-Russjan
, 
Diana Wierzbicka
Diana Wierzbicka
, 
Karolina Stępieñ-Hołubczat
Karolina Stępieñ-Hołubczat
, 
Paulina Lewandowska
Paulina Lewandowska
, 
Ewa Frankiewicz
Ewa Frankiewicz
, 
Andrzej Cacko
Andrzej Cacko
, 
Beata Leszczyñska
Beata Leszczyñska
, 
Urszula Demkow
Urszula Demkow
 and 
Edyta Podsiadły
Edyta Podsiadły
   | May 10, 2024
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Article Category: Original Paper
Published Online: May 10, 2024
Page range: 177 - 187
DOI: https://doi.org/10.33073/pjm-2024-016
Keywords
© 2024 Dominika Seliga-Gąsior et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Antibiotic susceptibility of STEC isolates (n = 33). AMC – amoxicillin/clavulanic acid, TZP – piperacillin/tazobactam, CTX – cefotaxime, CXM – cefuroxime, CAZ – ceftazidime, FEP – cefepime, IPM – imipenem, MEM – meropenem, AK – amikacin, CN – gentamicin, TOB – tobramycin, CIP – ciprofloxacin, SXT – trimethoprim/sulfamethoxazole, AZT – azithromycin.
Antibiotic susceptibility of STEC isolates (n = 33). AMC – amoxicillin/clavulanic acid, TZP – piperacillin/tazobactam, CTX – cefotaxime, CXM – cefuroxime, CAZ – ceftazidime, FEP – cefepime, IPM – imipenem, MEM – meropenem, AK – amikacin, CN – gentamicin, TOB – tobramycin, CIP – ciprofloxacin, SXT – trimethoprim/sulfamethoxazole, AZT – azithromycin.

Fig. 2.

Percentage of STEC isolates by month, 2018–2022 (n = 45).
Percentage of STEC isolates by month, 2018–2022 (n = 45).

Fig. 3.

PFGE patterns of selected Escherichia coli isolates. Original designations of the isolates and their PFGE types are shown above the corresponding lanes. Salmonella Branderup and γ ladder (New England Biolabs, USA) were used as PFGE molecular weight markers. Results of the analysis of genetic relatedness of STEC strains, 2018-2022.
PFGE patterns of selected Escherichia coli isolates. Original designations of the isolates and their PFGE types are shown above the corresponding lanes. Salmonella Branderup and γ ladder (New England Biolabs, USA) were used as PFGE molecular weight markers. Results of the analysis of genetic relatedness of STEC strains, 2018-2022.

Characteristics of primers and probes used in the study.

Gene name GenBank Access No. Nucleotide sequences (5’→3’)* Amplicon size (bp)
sxt1 M16625 Fw**: TTTGTYACTGTSACAGCWGAAGCYTTACG 132
Rv**: CCCCAGTTCARWGTRAGRTCMACRTC
Probe: CTGGATGATCTCAGTGGGCGTTCTTATGTAA
sxt2 X07865 Fw: TTTGTYACTGTSACAGCWGAAGCYTTACG 128
Rv: CCCCAGTTCARWGTRAGRTCMACRTC
Probe: TCGTCAGGCACTGTCTGAAACTGCTCC
uh Z11541 Fw: CATTGATCAGGATTTTTCTGGTGATA 102
Rv: CTCATGCGGAAATAGCCGTTA
Poll: ATAGTCTCGCCAGTATTCGCCACCAATACC

Characteristics of patients infected with STEC as diagnosed in the Medical Center of the Medical University of Warsaw, 2018–2022.

Characteristic of STEC patients Value (No.)
Boys 26
Girls 19
Median age (range) 2 years 8 months (1 month–16 years and 6 months)
Clinical manifestation Value (No.)
HUS 20
Only diarrhea 4
No data 21
Laboratory results No. cases/No. total
Direct positive PCR for stx1 and/or -2 from stool 45/45
Positive culture of STEC 44/45
PCR gene detection results No. cases/No. total
stx11 positive, stx22 negative 3/45
stx1 negative, stx2 positive 38/45
stx1 positive, stx2 positive 3/45
stx – unknown stx type 1/45
eaeA3 44/45
Serotypes No. total
O157 40
other than O157 1

Molecular characteristic of selected isolates derived from gastrointestinal infections.

PFGE type/subtype No. of isolates (Total No. 24) stx1, stx2 gene presence Voivodeship
B1, D, E, F, G, I, J, M, N, O, Q 11 stx2 Podlaskie
A1, A2, B2, C1, C2, H, K 8 stx2 Mazovian
Citrobacter freundii (no PFGE type) 1 stx1 Mazovian
C1, P, R 3 stx2 Warmian-Masurian
L 1 stx2 Greater Poland
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