RPA-Cas12a | Quantitative Real-Time PCR | Total | |
---|---|---|---|
+ | − | ||
+ | 24 | 0 | 24 |
− | 0 | 11 | 11 |
Total | 24 | 11 | 35 |
Name | Source of strains |
---|---|
CMCC(B)51572 | |
NCTC12900 | |
ATCC® 17802™ | |
Clinical isolates | |
ATCC® 33291™ | |
CMCC(B)50024 | |
Human astrovirus | Clinical isolates |
Rotavirus | ATCC® VR-2274™ |
Enteric adenovirus | ATCC® VR-930™ |
Oligonucleotide | Sequence (5’–3’) |
---|---|
RPA forward primers | CCTCTCTTCACGGACCCTCTTTCTACAGC |
RPA reverse primers | TTCATTCACAAAATTGGGAGCCAGATTGC |
crRNA | AGUGCCUGGGAGAAAGAUGUCGUUU |
ssDNA reporters | FAM-TTATTATT-BHQ1 |
FAM-TTATTATT-Biotin |