Susceptibility of Clostridium sporogenes Spores to Selected Reference Substances and Disinfectants
Agnieszka Chojecka
Department of Bacteriology and Biocontamination Control, National Institute of Public Health NIH – National Research InstituteWarsaw, Poland
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Sep 24, 2022
About this article
Article Category: Original Paper
Published Online: Sep 24, 2022
Page range: 353 - 358
Received: Mar 22, 2022
Accepted: Jul 06, 2022
DOI: https://doi.org/10.33073/pjm-2022-031
Keywords
© 2022 Agnieszka Chojecka, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Sporicidal activity of peracetic acid-based disinfectant B_ Composition: 1% product solution contains 0_15% peracetic acid (results from 1 experiment, the reproducibility of PN‑EN 13704: 2018-09 method was ± 0_09)_
| Disinfectant | Concentration [%] | Contact time [min] | Organic load conditions | The reduction required [lg] | The reduction obtained [lg] |
|---|---|---|---|---|---|
| B | 1 | 15 | clean* | ≥ 3 | > 3.31 |
| B | 1 | 15 | dirty** | ≥ 3 | > 3.31 |
The susceptibility of Clostridium sporogenes ATCC 3584 spores to glutardialdehyde and peracetic acid under the parameters defined for Clostridioides difficile R027 spores (results from two independent experiments, the reproducibility of PN-EN 13704: 2018-09 method was ± 0_09)_
| Reference substance | Concentration [%] | Contact time [min] | The reduction required [lg] | The reduction obtained [lg] |
|---|---|---|---|---|
| Glutardialdehyde | 1.0 | 15 | < 1.5 | < 1.82 |
| Glutardialdehyde | 6.0 | 15 | ≥ 1.5 | 2.11 |
| Peracetic acid | 0.01 | 15 | < 1.5 | 2.54 |
| Peracetic acid | 0.04 | 15 | ≥ 1.5 | > 3.38 |
Sporicidal activity of the glutaraldehyde-based disinfectant A_ Composition: 2_5% glutardialdehyde (results from three independent experiments, the reproducibility of PN‑EN 13704: 2018-09 method was ± 0_09)_
| Disinfectant | Concentration [%] | Contact time [min] | Organic load conditions | The reduction required [lg] | The reduction obtained [lg] |
|---|---|---|---|---|---|
| A | 80 | 15 | clean* | ≥ 3 | < 1.84 |
| A | 80 | 60 | clean | ≥ 3 | > 3.19 |
| A | 80 | 60 | dirty** | ≥ 3 | 3.24 |
The composition of neutralizers used in the tests_
| Disinfectants/reference substances | Composition |
|---|---|
| A) Glutardialdehyde | polysorbate 80 – 30 g/l |
| saponin – 30 g/l | |
| histidine – 1 g/l in diluent | |
| B) Peracetic acid | polysorbate 80 – 10 g/l |
| sodium thiosulfate – 5 g/l | |
| lecithin – 3 g/l in diluent | |