Susceptibility of Clostridium sporogenes Spores to Selected Reference Substances and Disinfectants

Sporicidal activity of peracetic acid-based disinfectant B_ Composition: 1% product solution contains 0_15% peracetic acid (results from 1 experiment, the reproducibility of PN‑EN 13704: 2018-09 method was ± 0_09)_

Disinfectant	Concentration [%]	Contact time [min]	Organic load conditions	The reduction required [lg]	The reduction obtained [lg]
B	1	15	clean*	≥ 3	> 3.31
B	1	15	dirty**	≥ 3	> 3.31

The susceptibility of Clostridium sporogenes ATCC 3584 spores to glutardialdehyde and peracetic acid under the parameters defined for Clostridioides difficile R027 spores (results from two independent experiments, the reproducibility of PN-EN 13704: 2018-09 method was ± 0_09)_

Reference substance	Concentration [%]	Contact time [min]	The reduction required [lg]	The reduction obtained [lg]
Glutardialdehyde	1.0	15	< 1.5	< 1.82
Glutardialdehyde	6.0	15	≥ 1.5	2.11
Peracetic acid	0.01	15	< 1.5	2.54
Peracetic acid	0.04	15	≥ 1.5	> 3.38

Sporicidal activity of the glutaraldehyde-based disinfectant A_ Composition: 2_5% glutardialdehyde (results from three independent experiments, the reproducibility of PN‑EN 13704: 2018-09 method was ± 0_09)_

Disinfectant	Concentration [%]	Contact time [min]	Organic load conditions	The reduction required [lg]	The reduction obtained [lg]
A	80	15	clean*	≥ 3	< 1.84
A	80	60	clean	≥ 3	> 3.19
A	80	60	dirty**	≥ 3	3.24

The composition of neutralizers used in the tests_

Disinfectants/reference substances	Composition
A) Glutardialdehyde	polysorbate 80 – 30 g/l
	saponin – 30 g/l
	histidine – 1 g/l in diluent
B) Peracetic acid	polysorbate 80 – 10 g/l
	sodium thiosulfate – 5 g/l
	lecithin – 3 g/l in diluent