<abstract xmlns="http://www.w3.org/1999/xhtml">Research on the susceptibility of the spores of anaerobic bacteria such as <italic>Clostridium sporogenes</italic> or <italic>Clostridioides difficile</italic> is vital for assessing the sporicidal activity of disinfectants. The diverse susceptibility of anaerobic bacteria spores may lead to different disinfection parameters being determined by laboratories that prepare spore suspensions to test sporicidal effectiveness. The tests were performed using the suspension method according to PN-EN 13704:2018-09. In order to assess the susceptibility of the <italic>C. sporogenes</italic> spores, the criterion established for the <italic>C. difficile</italic> ribotype 027 spores was used in accordance with PN‑EN 17126:2019-01. The susceptibility of the <italic>C. sporogenes</italic> spores to glutardialdehyde corresponded to the susceptibility ranges established for the <italic>C. difficile</italic> ribotype 027 spores. The <italic>C. sporogenes</italic> spore suspension was susceptible to low concentrations of peracetic acid (0.01%). A disinfectant containing peracetic acid as the active substance showed high sporicidal activity at a low concentration (1%), a short contact time (15 minutes), and a high organic load (3.0 g/l bovine albumin + 3.0 ml/l sheep erythrocytes), as compared to a disinfectant with glutardialdehyde, which was sporicidal at a higher concentration (2.5%), at a longer contact time
(60 minutes) and lower organic conditions (3.0 g/l bovine albumin). There is a need to define the minimum susceptibility criteria for the <italic>C. sporogenes</italic> spores to the reference substances most often found in disinfectants with sporicidal activity. Excessive susceptibility of the <italic>C. sporogenes</italic> spores to reference substances may result in low-performance parameters of disinfection products with sporicidal activity and lead to ineffective disinfection in practice.<fig id="j_pjm-2022-031_fig_001" position="float" fig-type="figure">
<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/j_pjm-2022-031_fig_001.jpg"></graphic>
</fig></abstract>

Research on the susceptibility of the spores of anaerobic bacteria such as Clostridium sporogenes or Clostridioides difficile is vital for assessing the sporicidal activity of disinfectants. The diverse susceptibility of anaerobic bacteria spores may lead to different disinfection parameters being determined by laboratories that prepare spore suspensions to test sporicidal effectiveness. The tests were performed using the suspension method according to PN-EN 13704:2018-09. In order to assess the susceptibility of the C. sporogenes spores, the criterion established for the C. difficile ribotype 027 spores was used in accordance with PN‑EN 17126:2019-01. The susceptibility of the C. sporogenes spores to glutardialdehyde corresponded to the susceptibility ranges established for the C. difficile ribotype 027 spores. The C. sporogenes spore suspension was susceptible to low concentrations of peracetic acid (0.01%). A disinfectant containing peracetic acid as the active substance showed high sporicidal activity at a low concentration (1%), a short contact time (15 minutes), and a high organic load (3.0 g/l bovine albumin + 3.0 ml/l sheep erythrocytes), as compared to a disinfectant with glutardialdehyde, which was sporicidal at a higher concentration (2.5%), at a longer contact time
(60 minutes) and lower organic conditions (3.0 g/l bovine albumin). There is a need to define the minimum susceptibility criteria for the C. sporogenes spores to the reference substances most often found in disinfectants with sporicidal activity. Excessive susceptibility of the C. sporogenes spores to reference substances may result in low-performance parameters of disinfection products with sporicidal activity and lead to ineffective disinfection in practice.

Susceptibility of <italic>Clostridium sporogenes</italic> Spores to Selected Reference Substances and Disinfectants

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Susceptibility of  Spores to Selected Reference Substances and Disinfectants

Research on the susceptibility of the spores of anaerobic bacteria such as Clostridium sporogenes or Clostridioides difficile is vital for assessing the...

Disinfectant	Concentration [%]	Contact time [min]	Organic load conditions	The reduction required [lg]	The reduction obtained [lg]
B	1	15	clean*	≥ 3	> 3.31
B	1	15	dirty**	≥ 3	> 3.31

Reference substance	Concentration [%]	Contact time [min]	The reduction required [lg]	The reduction obtained [lg]
Glutardialdehyde	1.0	15	< 1.5	< 1.82
Glutardialdehyde	6.0	15	≥ 1.5	2.11
Peracetic acid	0.01	15	< 1.5	2.54
Peracetic acid	0.04	15	≥ 1.5	> 3.38

Disinfectant	Concentration [%]	Contact time [min]	Organic load conditions	The reduction required [lg]	The reduction obtained [lg]
A	80	15	clean*	≥ 3	< 1.84
A	80	60	clean	≥ 3	> 3.19
A	80	60	dirty**	≥ 3	3.24

Disinfectants/reference substances	Composition
A) Glutardialdehyde	polysorbate 80 – 30 g/l
	saponin – 30 g/l
	histidine – 1 g/l in diluent

B) Peracetic acid	polysorbate 80 – 10 g/l
	sodium thiosulfate – 5 g/l
	lecithin – 3 g/l in diluent

Susceptibility of Clostridium sporogenes Spores to Selected Reference Substances and Disinfectants

Article Category: Original Paper

Publicado en línea: 24 sept 2022

Páginas: 353 - 358

Recibido: 22 mar 2022

Aceptado: 06 jul 2022

DOI: https://doi.org/10.33073/pjm-2022-031

Palabras clave
glutardialdehyde, peracetic acid, susceptibility of spores,

© 2022 Agnieszka Chojecka, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Sporicidal activity of peracetic acid-based disinfectant B. Composition: 1% product solution contains 0.15% peracetic acid (results from 1 experiment, the reproducibility of PN‑EN 13704: 2018-09 method was ± 0.09).

The susceptibility of Clostridium sporogenes ATCC 3584 spores to glutardialdehyde and peracetic acid under the parameters defined for Clostridioides difficile R027 spores (results from two independent experiments, the reproducibility of PN-EN 13704: 2018-09 method was ± 0.09).

Sporicidal activity of the glutaraldehyde-based disinfectant A. Composition: 2.5% glutardialdehyde (results from three independent experiments, the reproducibility of PN‑EN 13704: 2018-09 method was ± 0.09).

The composition of neutralizers used in the tests.

Susceptibility of Clostridium sporogenes Spores to Selected Reference Substances and Disinfectants

Article Category: Original Paper

Publicado en línea: 24 sept 2022

Páginas: 353 - 358

Recibido: 22 mar 2022

Aceptado: 06 jul 2022

DOI: https://doi.org/10.33073/pjm-2022-031

Palabras claveglutardialdehyde, peracetic acid, susceptibility of spores,

© 2022 Agnieszka Chojecka, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Sporicidal activity of peracetic acid-based disinfectant B. Composition: 1% product solution contains 0.15% peracetic acid (results from 1 experiment, the reproducibility of PN‑EN 13704: 2018-09 method was ± 0.09).

The susceptibility of Clostridium sporogenes ATCC 3584 spores to glutardialdehyde and peracetic acid under the parameters defined for Clostridioides difficile R027 spores (results from two independent experiments, the reproducibility of PN-EN 13704: 2018-09 method was ± 0.09).

Sporicidal activity of the glutaraldehyde-based disinfectant A. Composition: 2.5% glutardialdehyde (results from three independent experiments, the reproducibility of PN‑EN 13704: 2018-09 method was ± 0.09).

The composition of neutralizers used in the tests.

Palabras clave
glutardialdehyde, peracetic acid, susceptibility of spores,