About this article
Article Category: original-paper
Published Online: Aug 05, 2020
Page range: 251 - 261
Received: Mar 14, 2020
Accepted: May 28, 2020
DOI: https://doi.org/10.33073/pjm-2020-027
Keywords
© 2020 Carlos Vegas et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Fig. 1.
Molecular methods for the identification of yeasts isolated from Amazonian native fruits_
| Strain profile | Method of identification | Identification consensus | |
|---|---|---|---|
| Restriction profile | D1/D2 26S ribosomal RNA sequencing | ||
| P01 | Not determined | ||
| P04 | Not determined | ||
| P05 | Not determined | ||
| P06 | |||
| P07 | Not determined | ||
| P08 | |||
| P09 | |||
| P10 | |||
| P11 | Not determined | ||
| P12 | Not determined | ||
| P13 | |||
| P14 | |||
| P15 | |||
| P16 | |||
| P17 | |||
| P18 | |||
| P19 | Not determined | ||
| P20 | |||
| P21 | |||
| P22 | |||
| P23 | Not determined | ||
| P24 | |||
| P25 | |||
| P26 | – | ||
| P27 | |||
| P28 | |||
| P29 | |||
| P30 | |||
| P32 | |||
Biochemical tests performed on yeasts isolated from ANF_
| Species | Strain profile | Fermentation of carbohydrates | Temperature (°C) | Osm | Acid prod | Tol | Tri | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Glu | Suc | Mal | Sta | Cel | 4 | 15 | 30 | 37 | ||||||
| P11 | + | + | + | – | – | ✓ | ✓✓✓ | ✓✓✓ | – | – | + | – | + | |
| P07 | + | + | – | – | – | – | ✓✓✓ | ✓✓✓ | – | + | + | – | – | |
| P12 | + | + | – | – | – | – | ✓✓ | ✓✓✓ | – | + | – | – | – | |
| P04 | + | + | + | – | + | ✓ | ✓✓✓ | ✓✓✓ | – | – | + | – | – | |
| P05 | – | – | – | – | – | – | ✓✓ | ✓✓ | ✓ | + | + | – | – | |
| P19 | + | + | – | – | – | – | ✓✓✓ | ✓✓✓ | ✓✓✓ | + | – | – | – | |
| P01 | – | – | – | – | – | – | ✓✓ | ✓✓ | ✓✓ | + | – | – | – | |
| P06 | – | – | – | – | – | – | ✓ | ✓✓ | ✓✓ | + | – | – | – | |
| P10 | + | + | + | – | – | ✓ | ✓✓✓ | ✓✓✓ | ✓✓✓ | + | + | + | – | |
| P15 | + | + | + | – | – | ✓ | ✓✓✓ | ✓✓✓ | ✓✓✓ | – | + | – | – | |
| P08 | – | – | – | – | – | – | ✓✓✓ | ✓✓✓ | ✓ | + | – | + | – | |
| P09 | – | – | – | – | – | – | ✓ | ✓✓ | – | + | – | – | – | |
| P20 | + | + | – | – | – | – | ✓✓ | ✓✓ | ✓✓ | + | – | – | – | |
| P22 | – | – | – | – | – | – | ✓ | ✓✓ | – | + | – | – | – | |
| P13 | + | + | – | – | – | – | ✓✓✓ | ✓✓✓ | – | + | + | + | – | |
| P14 | + | + | – | – | – | – | ✓✓ | ✓✓✓ | ✓ | + | + | – | – | |
| P18 | + | + | – | – | + | – | ✓✓✓ | ✓✓✓ | – | – | + | – | – | |
| P24 | – | – | – | – | – | – | ✓✓ | ✓✓✓ | – | + | – | + | – | |
| P27 | + | – | – | – | – | – | ✓✓ | ✓✓✓ | – | + | + | – | – | |
| P30 | + | – | – | – | + | ✓ | ✓✓✓ | ✓✓✓ | ✓ | – | + | – | – | |
| P16 | + | – | – | – | + | – | ✓✓✓ | ✓✓✓ | – | – | + | + | – | |
| P17 | + | – | – | – | – | – | ✓✓ | ✓✓✓ | ✓✓ | – | + | – | – | |
| P26 | – | – | – | – | – | – | ✓✓✓ | ✓✓✓ | – | + | – | – | – | |
| P28 | + | – | – | – | – | – | ✓✓✓ | ✓✓✓ | ✓✓ | – | + | – | – | |
| P29 | + | – | – | – | – | – | ✓✓ | ✓✓✓ | – | – | + | – | – | |
| P25 | – | – | – | – | – | – | ✓✓ | ✓ | ✓ | + | – | – | – | |
| P21 | + | + | – | – | – | – | ✓ | ✓✓ | – | + | – | – | – | |
| P32 | – | – | + | + | – | – | ✓✓ | ✓✓✓ | – | – | – | + | – | |
| P23 | – | – | – | – | – | – | ✓✓ | ✓✓ | – | + | – | – | – | |
Source and incidence of yeast species and strains isolated from ANF_
| Species | Restriction profile | Strain profile | Number of isolates from each fruit | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Total (%) | |||||||||||||
| I | P11 | 4 | 4.9 | ||||||||||
| II | P07, P12 | 1 | 1 | 1 | 3.7 | ||||||||
| III | P04 | 1 | 1.2 | ||||||||||
| IV | P05 | 1 | 1.2 | ||||||||||
| V | P19 | 1 | 1.2 | ||||||||||
| VI | P01 | 6 | 7.4 | ||||||||||
| VII | P06, P10, P15 | 4 | 6 | 1 | 2 | 16.0 | |||||||
| VIII | P08, P09 | 9 | 11.1 | ||||||||||
| VIII | P20, P22 | 5 | 6.2 | ||||||||||
| IX | P13, P14, P18, P24, P27 | 1 | 7 | 4 | 4 | 4 | 24.7 | ||||||
| X | P30 | 3 | 3.7 | ||||||||||
| IX | P16, P17, P26, P28, P29 | 4 | 4 | 1 | 11.1 | ||||||||
| IX | P25 | 1 | 1.2 | ||||||||||
| XI | P21 | 1 | 1.2 | ||||||||||
| XII | P32 | 2 | 2.5 | ||||||||||
| II | P23 | 2 | 2.5 | ||||||||||