Yeasts Associated with Various Amazonian Native Fruits

Strain profile	Method of identification		Identification consensus
Strain profile	Restriction profile	D1/D2 26S ribosomal RNA sequencing	Identification consensus
P01	Not determined	C. quercitrusa	Candida quercitrusa
P04	Not determined	C. intermedia	Candida intermedia
P05	Not determined	C. jaroonii	Candida jaroonii
P06	C. tropicalis	C. tropicalis	Candida tropicalis
P07	Not determined	C. carpophila	Candida carpophila
P08	D. hansenii	D. hansenii	Debaryomyces hansenii
P09	D. hansenii	D. hansenii	Debaryomyces hansenii
P10	–	C. tropicalis	Candida tropicalis
P11	Not determined	C. akabanensis	Candida akabanensis
P12	Not determined	C. carpophila	Candida carpophila
P13	H. guilliermondii/H. uvarum	H. opuntiae	Hanseniaspora opuntiae
P14	H. guilliermondii/H. uvarum	H. opuntiae	Hanseniaspora opuntiae
P15	C. tropicalis	C. tropicalis	Candida tropicalis
P16	H. guilliermondii/H. uvarum	H. thailandica	Hanseniaspora thailandica
P17	H. guilliermondii/H. uvarum	H. thailandica	Hanseniaspora thailandica
P18	H. guilliermondii/H. uvarum	H. opuntiae	Hanseniaspora opuntiae
P19	Not determined	C. pseudohaemulonii	Candida pseudohaemulonii
P20	D. hansenii	D. nepalensis	Debaryomyces nepalensis
P21	C. incommunis	K. ohmeri	Kodamaea ohmeri
P22	D. hansenii	D. nepalensis	Debaryomyces nepalensis
P23	Not determined	Meyerozyma caribbica	Meyerozyma caribbica
P24	H. guilliermondii/H. uvarum	H. opuntiae	Hanseniaspora opuntiae
P25	H. guilliermondii/H. uvarum	H. uvarum	Hanseniaspora uvarum
P26	–	H. thailandica	Hanseniaspora thailandica
P27	H. guilliermondii/H. uvarum	H. opuntiae	Hanseniaspora opuntiae
P28	H. guilliermondii/H. uvarum	H. thailandica	Hanseniaspora thailandica
P29	H. guilliermondii/H. uvarum	H. thailandica	Hanseniaspora thailandica
P30	H. guilliermondii/H. uvarum	H. pseudoguillermondii	Hanseniaspora pseudoguillermondii
P32	C. sake	Martiniozyma asiatica	Martiniozyma asiatica

Biochemical tests performed on yeasts isolated from ANF.

Species	Strain profile	Fermentation of carbohydrates^a					Temperature (°C)^b				Osm^c 50%	Acid prod^d	Tol^e	Tri^f
Species	Strain profile	Glu	Suc	Mal	Sta	Cel	4	15	30	37	Osm^c 50%	Acid prod^d	Tol^e	Tri^f
C. akabanensis	P11	+	+	+	–	–	✓	✓✓✓	✓✓✓	–	–	+	–	+
C. carpophila	P07	+	+	–	–	–	–	✓✓✓	✓✓✓	–	+	+	–	–
C. carpophila	P12	+	+	–	–	–	–	✓✓	✓✓✓	–	+	–	–	–
C. intermedia	P04	+	+	+	–	+	✓	✓✓✓	✓✓✓	–	–	+	–	–
C. jaroonii	P05	–	–	–	–	–	–	✓✓	✓✓	✓	+	+	–	–
C. pseudohaemulonii	P19	+	+	–	–	–	–	✓✓✓	✓✓✓	✓✓✓	+	–	–	–
C. quercitrusa	P01	–	–	–	–	–	–	✓✓	✓✓	✓✓	+	–	–	–
C. tropicalis	P06	–	–	–	–	–	–	✓	✓✓	✓✓	+	–	–	–
	P10	+	+	+	–	–	✓	✓✓✓	✓✓✓	✓✓✓	+	+	+	–
	P15	+	+	+	–	–	✓	✓✓✓	✓✓✓	✓✓✓	–	+	–	–
D. hansenii	P08	–	–	–	–	–	–	✓✓✓	✓✓✓	✓	+	–	+	–
D. hansenii	P09	–	–	–	–	–	–	✓	✓✓	–	+	–	–	–
D. nepalensis	P20	+	+	–	–	–	–	✓✓	✓✓	✓✓	+	–	–	–
D. nepalensis	P22	–	–	–	–	–	–	✓	✓✓	–	+	–	–	–
H. opuntiae	P13	+	+	–	–	–	–	✓✓✓	✓✓✓	–	+	+	+	–
	P14	+	+	–	–	–	–	✓✓	✓✓✓	✓	+	+	–	–
	P18	+	+	–	–	+	–	✓✓✓	✓✓✓	–	–	+	–	–
	P24	–	–	–	–	–	–	✓✓	✓✓✓	–	+	–	+	–
	P27	+	–	–	–	–	–	✓✓	✓✓✓	–	+	+	–	–
H. pseudoguillermondii	P30	+	–	–	–	+	✓	✓✓✓	✓✓✓	✓	–	+	–	–
H. thailandica	P16	+	–	–	–	+	–	✓✓✓	✓✓✓	–	–	+	+	–
	P17	+	–	–	–	–	–	✓✓	✓✓✓	✓✓	–	+	–	–
	P26	–	–	–	–	–	–	✓✓✓	✓✓✓	–	+	–	–	–
	P28	+	–	–	–	–	–	✓✓✓	✓✓✓	✓✓	–	+	–	–
	P29	+	–	–	–	–	–	✓✓	✓✓✓	–	–	+	–	–
H. uvarum	P25	–	–	–	–	–	–	✓✓	✓	✓	+	–	–	–
K. ohmeri	P21	+	+	–	–	–	–	✓	✓✓	–	+	–	–	–
M. asiatica	P32	–	–	+	+	–	–	✓✓	✓✓✓	–	–	–	+	–
M. caribbica	P23	–	–	–	–	–	–	✓✓	✓✓	–	+	–	–	–

Source and incidence of yeast species and strains isolated from ANF.

Species	Restriction profile	Strain profile	Number of isolates from each fruit
Species	Restriction profile	Strain profile	C. medica/cidra	G. crophylla/charichuelo	M. flexuosa/aguaje	M. dubia/camu camu	O. bataua/ungurahui	P. sericea/umarí	S. sessiliflorum/cocona	S. dulcis/taperiba	S. mombin/ubos	S. jambos/pomarrosa	Total (%)
Candida akabanensis	I	P11			4								4.9
Candida carpophila	II	P07, P12	1		1		1						3.7
Candida intermedia	III	P04				1							1.2
Candida jaroonii	IV	P05				1							1.2
Candida pseudohaemulonii	V	P19	1										1.2
Candida quercitrusa	VI	P01				6							7.4
Candida tropicalis	VII	P06, P10, P15			4		6		1	2			16.0
Debaryomyces hansenii	VIII	P08, P09		9									11.1
Debaryomyces nepalensis	VIII	P20, P22	5										6.2
Hanseniaspora opuntiae	IX	P13, P14, P18, P24, P27						1	7	4	4	4	24.7
Hanseniaspora pseudoguillermondii	X	P30						3					3.7
Hanseniaspora thailandica	IX	P16, P17, P26, P28, P29								4	4	1	11.1
Hanseniaspora uvarum	IX	P25										1	1.2
Kodamaea ohmeri	XI	P21	1										1.2
Martiniozyma asiatica	XII	P32						2					2.5
Meyerozyma caribbica	II	P23	2										2.5

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Yeasts Associated with Various Amazonian Native Fruits

Article Category: original-paper

Pubblicato online: 05 ago 2020

Pagine: 251 - 261

Ricevuto: 14 mar 2020

Accettato: 28 mag 2020

DOI: https://doi.org/10.33073/pjm-2020-027

Parole chiave
yeast diversity, fruit, Amazonia, PCR-RFLP, 5.8S-ITS

© 2020 Carlos Vegas et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Molecular methods for the identification of yeasts isolated from Amazonian native fruits.

Biochemical tests performed on yeasts isolated from ANF.

Source and incidence of yeast species and strains isolated from ANF.

Yeasts Associated with Various Amazonian Native Fruits

Article Category: original-paper

Pubblicato online: 05 ago 2020

Pagine: 251 - 261

Ricevuto: 14 mar 2020

Accettato: 28 mag 2020

DOI: https://doi.org/10.33073/pjm-2020-027

Parole chiaveyeast diversity, fruit, Amazonia, PCR-RFLP, 5.8S-ITS

© 2020 Carlos Vegas et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Molecular methods for the identification of yeasts isolated from Amazonian native fruits.

Biochemical tests performed on yeasts isolated from ANF.

Source and incidence of yeast species and strains isolated from ANF.

Parole chiave
yeast diversity, fruit, Amazonia, PCR-RFLP, 5.8S-ITS