<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Cellulase is an important enzyme in present-day of industrial biotechnology. The current study is concerned with the production and partial characterization of cellulase enzyme from Bacillus sp. The effect of various fermentation conditions for cellulase production through shake-flask culture was investigated. Maximum enzyme production was obtained after 24 hours of incubation in fermentation medium with pH 3.5 at 35°C under having agitation at 150 rpm while inoculums volume 1% was applied. Enzyme production was 1.91 times higher after optimizing the production condition as compared to the basal media. Cellulase characterization revealed that optimum activity was at pH 5.5 and 50°C for 50 minutes. About 68% of the activity retained after heating the crude enzyme solution at 50°C for 30 minutes. This nature makes cellulase a suitable candidate for current mainstream biomass conversions for sustainable agriculture and industrial processes.</p></abstract>

Cellulase is an important enzyme in present-day of industrial biotechnology. The current study is concerned with the production and partial characterization of cellulase enzyme from Bacillus sp. The effect of various fermentation conditions for cellulase production through shake-flask culture was investigated. Maximum enzyme production was obtained after 24 hours of incubation in fermentation medium with pH 3.5 at 35°C under having agitation at 150 rpm while inoculums volume 1% was applied. Enzyme production was 1.91 times higher after optimizing the production condition as compared to the basal media. Cellulase characterization revealed that optimum activity was at pH 5.5 and 50°C for 50 minutes. About 68% of the activity retained after heating the crude enzyme solution at 50°C for 30 minutes. This nature makes cellulase a suitable candidate for current mainstream biomass conversions for sustainable agriculture and industrial processes.

Optimization of fermentation condition for cellulase enzyme production from Bacillus sp.

Department of Biotechnology and Genetic Engineering

Mawlana Bhashani Science and Technology University

Microbial Biotechnology Division, National Institute of Biotechnology

Biotechnology and Genetic Engineering Department

Mawlana Bhashani Science and Technology University (MBSTU)

Department of Nutrition and Food Engineering

Malaysian Journal of Halal Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Cellulase is an important enzyme in present-day of industrial biotechnology. The current study is concerned with the production and partial characterization of...

Optimization of fermentation condition for cellulase enzyme production from Bacillus sp.

Published Online: Jan 28, 2020

Page range: 19 - 24

DOI: https://doi.org/10.2478/mjhr-2019-0009

Keywords
Production of cellulase enzyme, Cellulase enzyme from Bacillus sp, Fermentation condition of enzyme production, Acidic cellulase

© 2019 Maidul Islam et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Optimization of fermentation condition for cellulase enzyme production from Bacillus sp.

Published Online: Jan 28, 2020

Page range: 19 - 24

DOI: https://doi.org/10.2478/mjhr-2019-0009

KeywordsProduction of cellulase enzyme, Cellulase enzyme from Bacillus sp, Fermentation condition of enzyme production, Acidic cellulase

© 2019 Maidul Islam et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
Production of cellulase enzyme, Cellulase enzyme from Bacillus sp, Fermentation condition of enzyme production, Acidic cellulase